FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22895055
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGC5174762.2602085908944094No Hit
TTACGT4062631.774457410126335No Hit
GACCGA2682981.1718600370254624No Hit
TACGGC2401621.048968871225686No Hit
TTAGTA2313551.0105020494600252No Hit
AACATA2262940.9883968394048408No Hit
TGCATA2262380.9881522451027089No Hit
AGTCAA2242350.9794036310460927No Hit
AATGTA2238010.9775080252045694No Hit
CCATGA2233890.9757085099817406No Hit
CCTCGG2160300.9435661980283515No Hit
ACAGTG2132900.9315985482454616No Hit
AAGAGG2066310.9025136650687233No Hit
CTGGAG2056450.8982070582490411No Hit
CATACG2018810.8817668269414509No Hit
TATGGT1991660.8699083710434414No Hit
GAGATA1988480.868519424827763No Hit
GCTGTC1968620.8598450626128654No Hit
GAAGAT1942670.8485107373622819No Hit
CGTACT1933050.8443089566720848No Hit
TAAGTC1867970.815883604560024No Hit
TCAAGC1847810.80707820968327No Hit
TGTGAT1824940.7970891530944128No Hit
GCCAAT1797590.785143342088499No Hit
GAGCAC1795670.7843047330526176No Hit
GACACG1785140.779705486621456No Hit
GGATTC1780850.7778317195569087No Hit
CACATT1760980.7691529895866159No Hit
AACTAG1721320.7518304716891923No Hit
TCTTCA1707940.7459864149703943No Hit
TGATAA1686570.7366525216908193No Hit
GTACGC1651370.7212780226996616No Hit
GAATAC1641770.7170849775202549No Hit
GACTAA1640910.7167093505562664No Hit
GGTACC1639190.7159580966282894No Hit
GGCTAC1637470.7152068427003124No Hit
ATGGCC1631740.712704118858854No Hit
CTATTA1611860.7040210211331661No Hit
TACTCG1583310.6915510794798265No Hit
GGAGGC1565670.6838463589626668No Hit
GTCTCA1558660.6807845624306209No Hit
CTTAGC1556230.6797231978695836No Hit
TGACCA1547650.675975663740489No Hit
CCTGCT1546610.6755214171793865No Hit
ACGAAC1529460.6680307166765924No Hit
TAGAGC1529170.6679040517701311No Hit
AGTGCA1525900.6664757957558958No Hit
ACTAAT1510790.6598761173537254No Hit
ACAGCC1504720.6572248898288299No Hit
CAGATC1497740.6541761965629697No Hit
TTAGCG1487600.6497472925922214No Hit
AGTAGG1481370.6470261809810023No Hit
CCTTAT1478810.6459080355998271No Hit
ATACCT1467780.6410904013989047No Hit
TTGAAT1462660.6388541106365545No Hit
CACCTA1442460.6300312447382197No Hit
CTTCAC1442100.6298740055439919No Hit
AACCTT1438130.6281400066520915No Hit
GAACCG1437860.6280220772564207No Hit
TTCTGT1436500.6274280625226714No Hit
TCGTGG1426770.623178236523127No Hit
CTCCGC1413890.6175525675740897No Hit
TCCAGA1412950.6171419985669394No Hit
CAGCCT1406690.6144077836895346No Hit
GTGGTG1394970.6092887743663424No Hit
AAGCAT1394700.6091708449706715No Hit
TTCTAC1390300.6072490325967769No Hit
GATCAG1383340.604209074841707No Hit
ACCAGG1370380.5985484638495081No Hit
GACCAT1357280.5928267042817762No Hit
GGCCAG1351360.5902409930878088No Hit
CGATGT1334680.5829555770885897No Hit
ATCAGC1322360.5775745024416845No Hit
CCGACT1320460.5767446289165936No Hit
CTCGTC1317440.5754255667872386No Hit
AGGTGT1310910.572573422514163No Hit
TGACAC1308380.5714683803991735No Hit
GGCGTT1290430.5636282594647621No Hit
CAAGGA1280250.559181884472433No Hit
AGTTCC1276030.5573386916956522No Hit
TTCCAT1273300.5561462944727584No Hit
GTACTT1272480.5557881385303508No Hit
GGCAGG1268870.5542113788326781No Hit
CCTGAC1267220.5534906991924675No Hit
ATGTGA1260230.5504376381712122No Hit
GCATAA1249490.5457466688767509No Hit
CCAGCG1247140.5447202463588753No Hit
ACACGA1222230.5338401676693941No Hit
ATGCCG1211340.5290836820440047No Hit
GATTCA1208890.528013581972177No Hit
CAGTAG1202530.52523568954082No Hit
AACAAT1197920.5232221543036258No Hit
TCCTCC1193690.5213745937714498No Hit
GCACAT1185080.5176139563761695No Hit
TAGCTT1173940.5127482768658996No Hit
CCGCTC1170200.5111147363480891No Hit
CATGAG1156470.5051178081904586No Hit
ACTTGA1141650.4986447946947496No Hit
TACGAA1131560.4942377295009774No Hit
CTGAGG1127700.4925517759184243No Hit
GTTGGC1116750.4877690837606636No Hit
TCGGAC1100490.4806671134880436No Hit
TGCGGA1095840.4786361072292685No Hit
GCGCAG1094260.4779460018768245No Hit
CGTCTC1092720.4772733675459613No Hit
GACTTG1086490.47455225593474226No Hit
GCGTAT1085430.47408927386284944No Hit
ATTGAA1083050.4730497480787882No Hit
GGTAAT1081320.47229412639541596No Hit
CTAGTT1074200.4691842845540227No Hit
ATTCAT1066060.4656289316623175No Hit
CTGTAT1058970.4625321930871099No Hit
TTAGGC1046060.45689342087188695No Hit
TATAAG1040770.45458287826781807No Hit
TCTCGC1040140.45430770967791956No Hit
CGACAA1032700.45105809966387944No Hit
AGCCTA1030430.45006661918916546No Hit
TGCGAC1001190.43729530241355613No Hit
GCGGCT999240.4364435901114892No Hit
GTTACA995460.4347925785720978No Hit
CCACTT995060.4346178683562891No Hit
CAGGAC993340.43386661442831215No Hit
AACTCT990210.43249950698960976No Hit
AGGTCA983920.4297521888460194No Hit
GGCCGT979680.42790026055844804No Hit
TCCACG975030.425869254299673No Hit
ATTGCT963770.4209511617246606No Hit
TACAGT953000.4162470891640138No Hit
AGAACG931800.4069874477261574No Hit
GTCGAG926740.40477736349617854No Hit
AGACGG925100.4040610516113633No Hit
GCCATA917150.40058868607216713No Hit
AATTGC914540.39944870191401594No Hit
GGTGGT894510.3907000878573998No Hit
AAGCGC887160.38748978764191655No Hit
ATTCGA879790.3842707519156429No Hit
CGAGAG870880.3803790818585061No Hit
TATTGG857600.3745787026936603No Hit
CAGTCC852990.372565167456466No Hit
CGAAGC846740.3698353203344565No Hit
AGAGGT839200.3665420327664642No Hit
CTACCG814260.3556488508107974No Hit
ACGTAA802390.3504643251566768No Hit
TCATGT797050.3481319437756319No Hit
GATCCT794450.34699632737287595No Hit
GTCTGC792220.3460223179197429No Hit
CGAGCC790910.34545014196296975No Hit
AGGCTT783070.34202582173312096No Hit
AGTTAG769110.3359284352014005No Hit
ACCGCT767550.3352470653597469No Hit
CTGTTC756060.3302285144106446No Hit
TATTCT747050.3262931667995556No Hit
CCGTCG728770.31830890993710215No Hit
TTCACA694710.303432335060999No Hit
CATAAC693420.3028688946150162No Hit
CCGGAA669750.29253041759454174No Hit
AGATGC665910.29085319952277905No Hit
GAGCCA660770.2886081732496384No Hit
TCAATG625570.2732336742584807No Hit
GCCTGG616750.26938131399990084No Hit
CGCTTG591490.2583483638715871No Hit
GCTCAA549120.23984218426206008No Hit
CGAATT547850.23928747932686775No Hit
ATCCAC523720.22874808555821335No Hit
ACGCTA460860.20129237514389023No Hit
ACCTGC384660.16801007903235No Hit
GAGAAG335690.1466211808619809No Hit
GGATCG326730.14270767202786802No Hit
GAGTGA291210.12719340486406344No Hit
CGTGAA258800.1130375096281708No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)