FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23371227
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGC5301572.2684174861679276No Hit
TTACGT4164201.7817635334250959No Hit
GACCGA2744991.1745168535652835No Hit
TACGGC2398771.0263774340987746No Hit
TGCATA2339991.0012268504345108No Hit
TTAGTA2332130.997863740744121No Hit
AGTCAA2303230.9854981084219498No Hit
AATGTA2293710.9814247236569993No Hit
AACATA2265510.9693586049204861No Hit
CCATGA2252220.9636721255584911No Hit
CATACG2195050.9392104231412411No Hit
CCTCGG2159180.9238624912590169No Hit
ACAGTG2139740.9155445711087399No Hit
CTGGAG2094970.8963885379231479No Hit
AAGAGG2065670.8838517549806006No Hit
GAGATA2035220.8708229140044722No Hit
TATGGT2004410.8576400374700054No Hit
GAAGAT1995750.8539346265388634No Hit
CGTACT1995140.8536736218427898No Hit
GCTGTC1973970.8446154752593863No Hit
TAAGTC1921740.8222674830037807No Hit
TCAAGC1895990.8112496618170711No Hit
TGTGAT1887810.8077496316303804No Hit
GAGCAC1846520.7900826088420604No Hit
GGATTC1829790.7829242341448313No Hit
GCCAAT1809630.7742982428778772No Hit
CACATT1801880.7709821996080908No Hit
GACACG1786130.764243143930783No Hit
TCTTCA1753390.7502344656530014No Hit
AACTAG1725370.7382453646956576No Hit
GTACGC1699880.7273387914121924No Hit
TGATAA1694860.7251908511264727No Hit
GGTACC1676130.7171767233273632No Hit
CTATTA1662760.711456013841293No Hit
GAATAC1651680.7067151416568758No Hit
GACTAA1645690.7041521611167441No Hit
GGCTAC1644360.7035830853039936No Hit
ATGGCC1636360.7001600728964722No Hit
GGAGGC1615370.6911789440922378No Hit
TACTCG1593620.6818726291092889No Hit
ACGAAC1570770.6720956499203058No Hit
GTCTCA1570180.6718432027552511No Hit
CTTAGC1568910.6712997995355572No Hit
AGTGCA1567240.6705852456954869No Hit
CCTGCT1562510.66856138960954No Hit
TGACCA1561340.6680607740449399No Hit
TAGAGC1543060.6602391906937535No Hit
ACTAAT1522130.6512837344825755No Hit
ACAGCC1511950.6469279511940045No Hit
CAGATC1506930.6447800109082847No Hit
TTAGCG1504570.643770222248066No Hit
TTGAAT1498020.6409676308394078No Hit
CCTTAT1492710.6386956063539154No Hit
AGTAGG1485830.6357518156834471No Hit
AACCTT1477740.6322902943863409No Hit
GAACCG1475480.6313232933812162No Hit
ATACCT1472070.62986423434251No Hit
CTTCAC1452440.6214650176475544No Hit
AAGCAT1443050.6174472568342262No Hit
CAGCCT1442190.6170792830004175No Hit
TTCTGT1440520.6163647291603475No Hit
CACCTA1437870.615230856300356No Hit
TTCTAC1436570.6146746167841338No Hit
TCGTGG1433300.6132754604625593No Hit
GTGGTG1432360.6128732565046756No Hit
CTCCGC1422570.6086843450709712No Hit
TCCAGA1418290.6068530334329473No Hit
GATCAG1376110.5888052005142905No Hit
ACCAGG1374640.5881762219844084No Hit
GACCAT1368280.5854549271204289No Hit
CTCGTC1360160.5819805695267947No Hit
GGCCAG1357750.5809493870390289No Hit
CGATGT1346440.5761101032478954No Hit
ATCAGC1344380.5752286775529586No Hit
CCGACT1326150.5674284880293191No Hit
AGGTGT1318620.5642065776007396No Hit
TGACAC1317690.5638086524083652No Hit
TTCCAT1315460.5628544876997686No Hit
GGCGTT1302930.5574931945164882No Hit
CAAGGA1295050.5541215272950795No Hit
ATGTGA1288440.551293263293365No Hit
CCAGCG1284000.5493934914071905No Hit
GTACTT1282760.5488629244840247No Hit
GGCAGG1280220.5477761180446367No Hit
CCTGAC1278460.547023055314982No Hit
AGTTCC1276350.5461202357924982No Hit
GCATAA1256040.5374300630429031No Hit
ACACGA1251990.5356971630115954No Hit
GATTCA1241030.531007636013291No Hit
CAGTAG1215350.5200197661851472No Hit
ATGCCG1208420.5170545816871317No Hit
TCCTCC1206190.5161004169785351No Hit
AACAAT1204680.5154543233866155No Hit
GCACAT1198630.5128656702534274No Hit
TAGCTT1179680.504757409613111No Hit
CCGCTC1176650.5034609436637623No Hit
CATGAG1168240.4998625018703554No Hit
TACGAA1164450.4982408497422921No Hit
CTGAGG1155530.4944241909079057No Hit
ACTTGA1143140.4891228004417569No Hit
TCGGAC1142330.48877622043549535No Hit
GTTGGC1127730.4825292227917687No Hit
GCGCAG1126990.482212594144073No Hit
GACTTG1112040.47581583970751723No Hit
TGCGGA1109500.47472903326812926No Hit
GCGTAT1092960.46765195511557867No Hit
CGTCTC1090800.4667277417655479No Hit
GGTAAT1088590.46578213458797013No Hit
ATTGAA1082900.4633475170131205No Hit
CTAGTT1082530.4631892026892726No Hit
ATTCAT1081660.46281695008995466No Hit
CTGTAT1069450.45759257740297504No Hit
TTAGGC1055930.45180768643426383No Hit
CGACAA1055710.4517135535930569No Hit
TCTCGC1042970.4462624063340791No Hit
AGCCTA1042140.4459072687967987No Hit
TATAAG1038270.4442513865446603No Hit
TGCGAC1033130.4420521010728277No Hit
CAGGAC1026620.4392666247262072No Hit
CCACTT1026100.4390441289197183No Hit
AGGTCA1017460.43534727551959507No Hit
GTTACA1005310.4301485754256719No Hit
TCCACG1003060.4291858531860565No Hit
GCGGCT1002000.4287323040420599No Hit
ATTGCT992580.4247017069322035No Hit
AACTCT990240.42370047580300346No Hit
GGCCGT963740.4123617472030886No Hit
TACAGT955000.4086221061478715No Hit
AGAACG953180.40784337082516037No Hit
AATTGC939500.4019900196082987No Hit
GCCATA930930.3983231175667413No Hit
AGACGG929580.39774548422297207No Hit
GTCGAG926530.3964404607426046No Hit
ATTCGA910180.38944467913473263No Hit
CGAGAG901170.3855895114107616No Hit
GGTGGT896890.3837581997727377No Hit
AAGCGC893230.3821921715962966No Hit
TATTGG880270.37664689149611186No Hit
CAGTCC873120.3735875741568896No Hit
CGAAGC866240.37064378348642113No Hit
AGAGGT845640.3618295265370534No Hit
CTACCG834970.35726408373852175No Hit
CGAGCC820730.3511711216531336No Hit
TCATGT817110.3496222085387301No Hit
GATCCT813970.34827867616877795No Hit
ACGTAA805870.3448128761061625No Hit
GTCTGC792810.33922480835088376No Hit
ACCGCT792410.3390536577305077No Hit
AGTTAG788980.33758604116078283No Hit
AGGCTT787030.33675168188644955No Hit
CTGTTC775930.33200225217101353No Hit
TATTCT772080.33035492744989386No Hit
CCGTCG747210.3197136376280116No Hit
TTCACA715740.3062483625699241No Hit
CATAAC694720.29725439746916155No Hit
CCGGAA687220.2940453233371102No Hit
GAGCCA686550.2937586460479803No Hit
AGATGC667820.2857445182488707No Hit
TCAATG645460.2761771985698483No Hit
GCCTGG628430.26889046090733704No Hit
CGCTTG608680.2604398990262685No Hit
CGAATT567780.24293974809281518No Hit
GCTCAA559130.2392386159271826No Hit
ATCCAC536210.22943168537963368No Hit
ACGCTA473890.20276641872504167No Hit
ACCTGC388030.16602893806131785No Hit
GAGAAG343870.14713390957179956No Hit
GGATCG333800.14282519270383193No Hit
GAGTGA294220.12588983881761964No Hit
CGTGAA264620.11322469290979031No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)