FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24548406
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCA10825064.4096793901811795No Hit
TAGAGC10589204.313599832103152No Hit
TTAGCG10462664.262052697026438No Hit
CTTCAC10271314.184104662437146No Hit
TGACAC9315873.794898129027197No Hit
GGCAGG9099753.7068598262551142No Hit
AGTTCC9072923.6959303997171955No Hit
CCTGAC9027193.6773018989501804No Hit
CAAGGA8951553.6464893076968012No Hit
GTACTT8882453.618340840541744No Hit
AACTCT6926462.821551835178219No Hit
TACAGT6652492.709947847530304No Hit
TGATAA2931971.194362680819276No Hit
ACTAAT2626601.0699676386320154No Hit
ATACCT2594811.0570177143069899No Hit
TTCTGT2548751.0382547852597843No Hit
GACCAT2389360.9733259259277365No Hit
TTAGTA2369390.9651909781840825No Hit
ATCAGC2362860.9625309276700084No Hit
CCATGA2272370.9256690638080535No Hit
AAGAGG2116040.8619867212559544No Hit
AACATA2052780.836217227301846No Hit
TACTCG2018020.8220574484551053No Hit
CTAGTT1922450.7831262037950651No Hit
CTTAGC1899610.7738221373721781No Hit
ATTGAA1895370.7720949376509416No Hit
ATTCAT1881730.7665385687363978No Hit
TATAAG1872000.7625749712629No Hit
CTGTAT1850540.7538330594662643No Hit
CTCCGC1796820.7319497648849379No Hit
GCTGTC1787430.7281246692758788No Hit
TCCAGA1732460.7057321766635275No Hit
AGGTGT1652480.67315164984643No Hit
GACACG1628710.6634687400884604No Hit
CCTGCT1570180.6396260514837501No Hit
GACTAA1540180.6274052987391523No Hit
CACCTA1478160.6021409292318206No Hit
TCGTGG1460110.5947881096638209No Hit
CGTCTC1384520.5639958863316828No Hit
GTCTGC1382680.5632463468300142No Hit
TCTCGC1327630.5408212655436773No Hit
GGTAAT1325700.5400350637837747No Hit
ACCAGG1252640.510273457266431No Hit
ACAGTG1217800.4960810897457048No Hit
GGCCGT1207080.4917142074316353No Hit
GCACAT1199180.4884960758755579No Hit
TACGGC1180050.4807033092087527No Hit
GTCGAG1157070.47134221260639086No Hit
TCCTCC1144440.4661972757009152No Hit
CCGCTC1114940.45418020216872734No Hit
AAGCGC1088940.4435888831234093No Hit
AGAGGT1077090.43876168578929314No Hit
CATGAG1060170.4318691812413401No Hit
TGCGGA1059870.4317469737138941No Hit
CCTCGG1048070.426940144301019No Hit
GCGTAT1041490.4242597258657038No Hit
GCCAAT1030710.41986840204614506No Hit
GTTGGC1020660.4157744498767048No Hit
AGTCAA1012240.41234449193972106No Hit
TATGGT983780.400751071169346No Hit
GCGGCT942960.3841227002681966No Hit
GAAGAT920020.37477789800282757No Hit
GAATAC915110.37277776813696173No Hit
CTGGAG911310.371229806122646No Hit
ATGGCC904060.36827645754270155No Hit
GGCTAC900980.3670217935942562No Hit
TGACCA888800.3620601679799495No Hit
GAGATA869790.3543162843241227No Hit
AACTAG840470.3423725353084025No Hit
CAGATC826990.33688134374182993No Hit
CCTTAT819840.3339687310043674No Hit
GGATTC817150.33287293684160185No Hit
AGGCTT815650.33226189920437194No Hit
CTATTA815210.3320826614974512No Hit
CGTACT804090.3275528358134536No Hit
CACATT776050.3161305055815029No Hit
CGATGT775440.31588201694236273No Hit
GGTACC768480.3130468023056161No Hit
GATCAG766710.3123257778936848No Hit
GGCCAG760970.3099875405352185No Hit
CCGACT745570.3037142207929916No Hit
AGTAGG739540.30125784949132745No Hit
GGCGTT736320.2999461553634073No Hit
ACAGCC721140.29376245447464083No Hit
TCTTCA720460.2934854507457633No Hit
CATAAC701920.28593302554960187No Hit
AGATGC684450.2788164738679978No Hit
CAGTAG679570.27682856475487655No Hit
AACAAT677160.2758468309510605No Hit
ATGCCG675900.2753335593357874No Hit
TGTGAT671900.2737041256365077No Hit
TAGCTT660220.268946179234611No Hit
ACTTGA638040.25991096937210506No Hit
GCATAA628560.2560492115048122No Hit
TTAGGC625470.25479047397211857No Hit
ACTAGC615430.2507005953869265No Hit
ACACGA586020.23872018411297255No Hit
AGCCTA585660.2385735350800374No Hit
ATGTGA561310.22865435743567222No Hit
AGACGG533140.2171790706084949No Hit
CCAGCG523010.21305252976506908No Hit
GTGGTG519730.2117163941316597No Hit
TTCTAC513690.20925594924574736No Hit
GGTGGT513340.20911337379706038No Hit
TTACGT503760.2052108800872855No Hit
GTTACA496910.202420474877269No Hit
CAGGAC474870.19344229519423786No Hit
TCCACG468330.19077817109591555No Hit
GCGCAG463360.18875359972456052No Hit
CGAGAG460140.18744190559664037No Hit
GATTCA459480.18717304903625923No Hit
TATTGG459380.1871323131937772No Hit
TGCGAC459000.18697751699234566No Hit
AGGTCA434070.1768220714615849No Hit
GATCCT429660.17502562080812906No Hit
AGAACG420230.17118423086207715No Hit
CTGAGG419150.17074428376327164No Hit
ACCTGC402530.16397398674276448No Hit
ACGTAA400950.163330360431549No Hit
TGCATA398960.16251971716615735No Hit
CATACG384020.15643378229934765No Hit
CGAGCC382630.15586755408884798No Hit
ATTGCT376440.15334600543921265No Hit
CTACCG372060.15156177553850136No Hit
CCACTT366880.14945165889793413No Hit
AATTGC363990.14827439305020457No Hit
TAAGTC356580.1452558671222889No Hit
GAGCCA354530.14442078235140807No Hit
GACCGA351930.14336165044687627No Hit
ACCGCT349310.14229437137384804No Hit
ATTCGA349080.14220067893613947No Hit
TCAAGC346320.1410763696836365No Hit
TATTCT341570.13914141716574185No Hit
TTCACA327490.1334058105442773No Hit
TCATGT296720.1208713918125682No Hit
AATGTA295470.1203621937815433No Hit
AGTGCA290810.11846390352188244No Hit
CCGGAA288500.11752290556054841No Hit
GAACCG270890.11034932369946952No Hit
ACGAAC268930.10955090118682248No Hit
AAGCAT266590.10859768247274385No Hit
AACCTT256700.1045689076512748No Hit
TTCCAT251260.1023528778202544No Hit
GCCTGG246290.10032830644889938No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)