FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28660012
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGC6462042.254723410443792No Hit
TTACGT5066061.767640571818323No Hit
GACCGA3379771.1792632885150223No Hit
TACGGC3088791.0777350686384919No Hit
TTAGTA2843580.9921768350969288No Hit
TGCATA2838110.9902682525045697No Hit
CATACG2835100.9892180087014618No Hit
AGTCAA2830370.9875676255822922No Hit
AATGTA2800110.9770093606380904No Hit
AACATA2757850.9622640772097374No Hit
CCATGA2740580.9562382597746295No Hit
CCTCGG2656930.9270512517580244No Hit
ACAGTG2597770.9064092506311582No Hit
CTGGAG2582300.901011485968673No Hit
AAGAGG2535730.8847623650680956No Hit
GAGATA2493910.870170605650828No Hit
TATGGT2445630.853324834616259No Hit
CGTACT2433080.8489459111182507No Hit
GAAGAT2422030.8450903649307614No Hit
GCTGTC2415400.8427770372182678No Hit
TAAGTC2339630.8163395046729219No Hit
TCAAGC2335080.8147519268310146No Hit
TGTGAT2298700.8020582824598956No Hit
GAGCAC2274000.793440002746684No Hit
GGATTC2257030.78751886077368No Hit
GCCAAT2205660.7695949324794421No Hit
GACACG2200910.7679375709961322No Hit
CACATT2193660.7654079139952907No Hit
TCTTCA2131100.7435795909645816No Hit
AACTAG2104650.7343507043890979No Hit
GTACGC2103330.7338901323558413No Hit
GGAGGC2070980.7226026283589833No Hit
TGATAA2064280.7202648763719988No Hit
GGTACC2064250.7202544088257884No Hit
CTATTA2020080.7048426916220412No Hit
GACTAA2019020.7044728383226079No Hit
GAATAC2017420.7039145691913876No Hit
GGCTAC2014870.7030248277635055No Hit
ATGGCC2013850.7026689311923526No Hit
TACTCG1948350.6798147886330264No Hit
GTCTCA1931490.6739320276627938No Hit
AGTGCA1930090.6734435421729761No Hit
ACGAAC1920650.670149754298777No Hit
CTTAGC1912770.6674002788275176No Hit
TGACCA1897860.6621979083609595No Hit
CCTGCT1897700.6621420814478375No Hit
TAGAGC1892560.6603486418637927No Hit
ACTAAT1850820.6457847959030861No Hit
TTAGCG1842820.6429934502469852No Hit
ACAGCC1830400.6386598861158885No Hit
AGTAGG1823570.6362767747619924No Hit
GAACCG1820190.6350974312222898No Hit
CAGATC1819990.6350276475808873No Hit
TTGAAT1817140.6340332306909013No Hit
AACCTT1801640.6286249984822058No Hit
CCTTAT1800230.628133023810318No Hit
ATACCT1797380.6271386069203321No Hit
GTGGTG1777020.6200346322255553No Hit
CTTCAC1764500.6156661762737573No Hit
TCGTGG1755110.6123898343099089No Hit
CAGCCT1754860.6123026047581557No Hit
TTCTGT1750660.6108371482887027No Hit
CACCTA1749450.6104149572582175No Hit
AAGCAT1744520.6086947904976453No Hit
TTCTAC1744010.6085168422120688No Hit
TCCAGA1727690.602822497073623No Hit
CTCCGC1723360.6013116812372584No Hit
GGCCAG1694450.5912244558725237No Hit
GATCAG1692330.590484749273657No Hit
ACCAGG1683740.5874875418754186No Hit
GACCAT1665550.5811407196898591No Hit
CTCGTC1655680.5776968969866447No Hit
ATCAGC1638230.5716082742742745No Hit
CGATGT1637950.5715105771763109No Hit
CCGACT1618450.564706672139565No Hit
GGCGTT1606640.560585948114746No Hit
AGGTGT1602370.5590960673708022No Hit
TGACAC1602080.5589948810907686No Hit
TTCCAT1588260.5541728314698543No Hit
ATGTGA1586350.5535063976944601No Hit
GGCAGG1580310.5513989317241039No Hit
CCAGCG1573200.5489181232722443No Hit
CAAGGA1571810.5484331269644968No Hit
GTACTT1571620.5483668325051644No Hit
AGTTCC1569080.5474805802593523No Hit
CCTGAC1556130.542962089478539No Hit
GCATAA1548060.5401463195479471No Hit
ACACGA1523780.5316745854816809No Hit
GATTCA1516490.529130971752559No Hit
ATGCCG1487320.518953027654001No Hit
CAGTAG1483130.5174910603666182No Hit
AACAAT1467570.5120618930655019No Hit
GCACAT1457470.5085378191746746No Hit
TCCTCC1453340.5070967869797123No Hit
CCGCTC1437930.5017199574096479No Hit
CATGAG1426260.49764808193381077No Hit
TACGAA1424890.49717006399020347No Hit
TAGCTT1419690.49535568931373786No Hit
CTGAGG1407140.4909767658157296No Hit
GTTGGC1405660.4904603668693509No Hit
TCGGAC1389050.4846648354508714No Hit
ACTTGA1388390.4844345494342431No Hit
GCGCAG1382750.4824666507466919No Hit
TGCGGA1379810.48144083121807485No Hit
GACTTG1371540.4785552776460806No Hit
CGTCTC1339220.4672782411954329No Hit
GCGTAT1335430.465955841190855No Hit
GGTAAT1330310.4641693799709505No Hit
TTAGGC1329790.4639879425033039No Hit
ATTGAA1327530.4631993873554554No Hit
CTAGTT1317730.4597799889267317No Hit
ATTCAT1313620.45834593509590993No Hit
CGACAA1301060.45396352241583154No Hit
CTGTAT1292220.45087908546584No Hit
TCTCGC1284310.44811914244837026No Hit
TATAAG1283810.44794468334486387No Hit
TGCGAC1277190.44563484481444043No Hit
AGCCTA1276600.44542898307230294No Hit
GCGGCT1266360.44185606063249383No Hit
CAGGAC1252970.4371840458405949No Hit
CCACTT1237670.4318455972733019No Hit
AGGTCA1235590.43111984740271564No Hit
GTTACA1228510.4286495064970664No Hit
TCCACG1218560.4251777703372909No Hit
ATTGCT1218380.42511496506002855No Hit
AACTCT1202730.41965439512028113No Hit
GGCCGT1190730.41546737663612987No Hit
AGAACG1165550.40668161618355214No Hit
TACAGT1156820.40363556023633207No Hit
GCCATA1151010.4016083454535888No Hit
AATTGC1151010.4016083454535888No Hit
AGACGG1150480.4014234188038721No Hit
GTCGAG1145830.39980094914126346No Hit
ATTCGA1119970.39077792430791725No Hit
GGTGGT1112900.38831107258433806No Hit
AAGCGC1104240.3852894409116088No Hit
CGAGAG1095210.3821387095022849No Hit
TATTGG1081840.3774736730745263No Hit
CAGTCC1067240.3723794672521421No Hit
CGAAGC1065480.3717653712077999No Hit
AGAGGT1036660.3617095484816964No Hit
CTACCG1023110.35698170677667546No Hit
CGAGCC1001820.34955323814937694No Hit
TCATGT997780.34814360859304594No Hit
GATCCT994400.3469642650533433No Hit
ACGTAA989740.3453383062086645No Hit
AGGCTT983880.3432936455155706No Hit
GTCTGC977520.3410745257189704No Hit
AGTTAG975140.3402441003862804No Hit
ACCGCT972550.3393404022301177No Hit
CTGTTC948650.3310012570825162No Hit
TATTCT937460.32709686234604507No Hit
CCGTCG926780.32337041589515036No Hit
TTCACA866350.30228528864537807No Hit
CATAAC848700.2961268822916055No Hit
CCGGAA846240.29526854350235443No Hit
GAGCCA839350.2928644970560375No Hit
AGATGC828170.2889635915016365No Hit
TCAATG784900.2738659006842007No Hit
GCCTGG776420.27090707428873373No Hit
CGCTTG751400.26217714074927817No Hit
GCTCAA702170.24499989741804712No Hit
CGAATT693940.24212830057433332No Hit
NNNNNN662480.23115133378171648No Hit
ATCCAC661840.23092802612922841No Hit
ACGCTA581290.20282266455436235No Hit
ACCTGC478260.16687362168585276No Hit
GAGAAG429730.14994062109953057No Hit
GGATCG418300.14595248599337643No Hit
GAGTGA361300.12606414819365744No Hit
CGTGAA330110.11518138931693399No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)