FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25470413
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC2913721.1439626047681284No Hit
AAGTTG2692351.0570499975795444No Hit
CTTAGC2615121.0267285418575662No Hit
CATTAA2563681.0065325599549564No Hit
CGAAGC2366990.9293096268207351No Hit
CTGTTC2355710.9248809589385143No Hit
AATCGG2279180.8948343318971702No Hit
TGATAA2243490.8808219953088314No Hit
CTATTA2172130.852805174380172No Hit
GACCGA2149140.8437790152833408No Hit
ACTAAT2136110.8386632756995341No Hit
GCGTAT2101310.8250003641480019No Hit
AATGTA2077990.8158446429588715No Hit
GACTAA2026470.7956172520641892No Hit
AACTAG1983390.7787035098331543No Hit
TGTGAT1976340.7759355924067662No Hit
CCACTT1929120.7573964348359801No Hit
TACGGC1891490.7426224301898834No Hit
TTCGTT1883270.7393951562544353No Hit
TCTTCA1866180.73268541032295No Hit
GGCTAC1862770.7313466020358602No Hit
TATAAG1854730.7281899983325751No Hit
GTGGTG1799650.706564907290667No Hit
ATGGCC1757450.6899966639724295No Hit
GAGATA1750780.6873779392583858No Hit
GTAAGA1718640.6747593766932637No Hit
ACTTGA1717880.6744609912685751No Hit
GCCAAT1706720.6700794368744629No Hit
GCCATA1704540.6692235418404876No Hit
GTCTCA1703730.6689055257957537No Hit
CTGTAT1698840.6669856511553228No Hit
TGTGTG1693470.664877322562457No Hit
CAAGGA1684820.6614812252946193No Hit
TGCGGA1676340.6581518721349355No Hit
GTACTT1672680.6567149107476192No Hit
TAGAGC1654310.6495026209429741No Hit
ACAGCC1649780.6477240867668694No Hit
TCTCTG1649610.6476573426587153No Hit
GAAGAT1629980.6399503612289286No Hit
AGCCTA1627100.638819637514319No Hit
CCTCGG1620500.6362283956683388No Hit
CCGACT1618710.6355256194707168No Hit
TAAGTC1606470.6307200436836261No Hit
CCTTAT1604870.6300918638421764No Hit
TTGAAT1603730.6296442857051434No Hit
TAGCTT1601760.6288708392753585No Hit
CAGATC1589140.6239160707759235No Hit
GCGCAG1577970.6195305902578022No Hit
ATTGCT1564000.614045795017144No Hit
ATGCCG1562740.6135511033920023No Hit
CTGAGG1562520.613464728663803No Hit
AACATA1545060.6066097161439824No Hit
GCTGTC1535520.6028641938393382No Hit
CCTGCT1533390.6020279294254082No Hit
AAGCGC1513560.5942424255154402No Hit
TATGGT1509170.5925188570754625No Hit
AGACGG1502720.589986507089618No Hit
ATTCGA1494510.586763159278179No Hit
CACCTA1494460.5867435286581336No Hit
AGGTCA1483960.5826210984486195No Hit
TTCTGT1477990.5802772024152102No Hit
CCGCTC1471470.5777173695613024No Hit
GAATAC1456210.5717261043234753No Hit
ATACCT1453660.5707249427011647No Hit
TTAGTA1453180.5705364887487298No Hit
CGATGT1451670.5699436440233616No Hit
AACCTT1444000.5669323069084118No Hit
TTACGT1435860.563736441965036No Hit
CGTCAG1433060.5626371272424989No Hit
CAGGAC1424460.5592606605947065No Hit
GAGTCG1422820.5586167762572205No Hit
TCCTCC1422690.5585657366451027No Hit
ATGTGA1404340.5513612990884758No Hit
TGACCA1400490.5498497413449872No Hit
TCGTGG1398810.549190152511465No Hit
CATACG1374750.5397438981456641No Hit
ATCCGG1374470.5396339666734105No Hit
TGCATA1365580.5361436424293552No Hit
ACGAAC1363000.5351307024350174No Hit
AGTCAA1356380.532531608341019No Hit
TCAAGC1356250.5324805687289013No Hit
GACACG1348010.529245442545435No Hit
GGACTA1339710.5259867596179143No Hit
GTTACA1335730.524424162262308No Hit
CTTCAC1335560.5243574181541539No Hit
TATTGG1332100.5229989792470189No Hit
ACACGA1326600.5208396110420354No Hit
AAGCAT1320700.5185231978766893No Hit
ATTGAA1309270.5140356381343325No Hit
GCACAT1308390.5136901392215353No Hit
CATAAC1290650.5067251952294609No Hit
CTGGAG1285210.5045893837685318No Hit
AGATGC1268970.4982133583778166No Hit
GGTGGT1267300.4975576956683035No Hit
TTCTAC1252550.4917666627549384No Hit
CATGAG1242710.4879033567300224No Hit
TAATGA1242190.48769919828155117No Hit
CGAGCC1237010.4856654660448576No Hit
GATTCA1236190.48534352387611457No Hit
AATTGC1236010.4852728536439515No Hit
GCGGCT1229940.4828896963704515No Hit
GGATTC1227860.4820730625765668No Hit
GCCTGG1224950.48093056048993005No Hit
CAGTAG1224240.4806518056852867No Hit
ACAGTG1212290.47596008749445873No Hit
TTCCAT1209630.4749157385080485No Hit
TCATGT1201260.4716295727124645No Hit
GGCGTT1186590.46586994879117194No Hit
TATTCT1180440.4634553825255994No Hit
TACTCG1171800.46006321138177064No Hit
CTAGTT1162360.45635695031721707No Hit
CCGGAA1159490.45523015272661654No Hit
CTCCGC1151210.45197932204711405No Hit
CGTACT1150440.45167701049841635No Hit
CGAGAG1147810.4506444398840333No Hit
ATCCAC1146830.45025967973114533No Hit
GCTCAA1146280.45004374291064697No Hit
GGCCAG1134740.44551299580419057No Hit
AACTCT1131730.44433123247746314No Hit
CAACGG1126770.4423838749689689No Hit
GTACGC1125800.44200304094008996No Hit
GATCCT1120400.43988293397519707No Hit
CACATT1119680.43960025304654465No Hit
AGTGCA1110250.4358979181060001No Hit
TCCAGA1106910.4345865926869737No Hit
CGTCTC1101000.43226625339761865No Hit
ATCAGC1100830.43219950928946466No Hit
ACCAGG1098340.4312219044112084No Hit
AAGGCG1097980.43108056394688227No Hit
AGAACG1097880.43104130270679164No Hit
ACGCTA1091580.4285678445810831No Hit
TACGAA1088500.4273585983862923No Hit
AACAAT1081680.4246809818121127No Hit
TGCGAC1067310.41903914161109207No Hit
ACCTGC1067060.41894098851086553No Hit
AGTTCC1066910.4188820966507296No Hit
TGGCAT1066760.4188232047905937No Hit
GCATAA1059950.41614951434042313No Hit
ACCGCT1058890.4157333451954627No Hit
CCTGAC1058670.4156469704672633No Hit
GACTTG1058160.4154467381428012No Hit
AGGTGT1057180.4150619779899133No Hit
GACCAT1051790.4129457971490293No Hit
ACGTAA1043180.4095654043772278No Hit
GTCTGC1041320.4088351453115424No Hit
TCCACG1036880.4070919462515194No Hit
GAACCG1034280.40607115400916355No Hit
TGACAC1033980.40595337028889167No Hit
CGAATT1029990.40438684680927633No Hit
GGTAAT1028190.4036801444876453No Hit
GAGCCA1017170.3993535558296601No Hit
AGTTAG1014900.3984623256796032No Hit
TCGGAC990110.38872946426114097No Hit
CAGCCT985750.38701767419319033No Hit
TTAGGC972400.38177629864109386No Hit
GGATCG968850.3803825246178772No Hit
GATCAG966030.379275357647322No Hit
TTCACA951950.37374737504256406No Hit
GGCAGG929720.36501960137042144No Hit
CGCTTG925650.3634216688987336No Hit
TACAGT908220.35657843475094025No Hit
CAGTCC894860.3513331330748347No Hit
GGAGGC888450.3488164875850266No Hit
GAGTGA876840.3442582576105067No Hit
CCATGA865200.3396882492639597No Hit
CGTGAA861120.33808639066826285No Hit
AAGAGG858250.33695959307766227No Hit
ATTCAT857580.3366965427690552No Hit
AGTAGG855740.335974135951388No Hit
AGAGGT854690.3355618929304366No Hit
GGCCGT844890.3317142914015568No Hit
CTATGC838020.32901704420733185No Hit
GTTGGC827140.32474542128547346No Hit
CCGTCG823750.32341446524640177No Hit
TTAGCG808660.3174899441167287No Hit
CAAGCT800570.31431370979339834No Hit
AGGCTT799690.31396821088060095No Hit
GAGAAG771970.30308499512748377No Hit
CGACAA753170.29570388199044906No Hit
CTACCG749690.2943375908352958No Hit
GTCGAG743820.29203295604197704No Hit
CCAGCG665190.2611618429587302No Hit
TCTCGC633110.24856683713766242No Hit
CTCGTC598730.23506882279451063No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)