FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25207316
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTAT8976473.561057432691366No Hit
GACTAA8591763.408439042062233No Hit
TGTGAT7324072.905533457032871No Hit
TGCGGA7183142.8496250850348366No Hit
CCACTT7167462.8434046687080845No Hit
GTGGTG6743252.675116224194595No Hit
GCTGTC6454482.5605582125443265No Hit
AACATA6377002.5298211043174925No Hit
CCGCTC6270892.487726182351187No Hit
TCCTCC5984412.3740766371159863No Hit
ATTGCT5824512.310642672151212No Hit
CTGAGG5812712.305961491497151No Hit
GACACG5632562.234494144477738No Hit
ATTCGA5575232.2117507472830504No Hit
GCGGCT5291462.0991762867573844No Hit
CATGAG5188252.0582318244433484No Hit
AATTGC4618431.8321784040791969No Hit
GCCTGG4602181.8257318629242398No Hit
GATTCA4595941.8232563911207367No Hit
TTCTAC4570041.8129815962952978No Hit
ACCAGG4534901.7990411989915942No Hit
TCATGT4422331.754383528972303No Hit
GTTGGC3514911.3944007366750193No Hit
CGCTTG3443251.3659724819572223No Hit
TACGGC3425471.3589189741581373No Hit
AACTAG3411761.3534800769744784No Hit
ACAGCC2837031.1254788094059676No Hit
CCTCGG2786351.105373535207001No Hit
TATGGT2608091.0346559705126877No Hit
GAGTCG2440680.9682427117587609No Hit
GTTACA2308370.9157539818995406No Hit
GGTACC2041850.8100227727537512No Hit
CAACGG1920190.7617590067899335No Hit
AATCGG1849970.7339020147960219No Hit
GCATAA1841530.7305537804976936No Hit
ACGTAA1806610.7167006594434727No Hit
CTATTA1511660.599690978603196No Hit
GAGTGA1510190.599107814572563No Hit
CTGTTC1506400.5976042828201146No Hit
AGTAGG1474980.5851396475531151No Hit
TGATAA1474370.5848976543159137No Hit
GAGATA1414030.5609601593442158No Hit
ACTAAT1401170.5558584658517393No Hit
GTAAGA1391570.5520500476925033No Hit
TATAAG1232440.48892154960091744No Hit
GAAGAT1131310.4488022445547158No Hit
CTGTAT1119660.44418057043439296No Hit
AGTCAA1097890.4355441888378755No Hit
GGACTA1084260.43013702847221025No Hit
CTGGAG1042250.41347123192330354No Hit
TAAGTC1020290.4047594753840512No Hit
GGATTC1000090.3967459288406588No Hit
CAGGAC995970.39511148271398666No Hit
TTCTGT976170.38725662026056246No Hit
ATACCT961830.3815677956352037No Hit
TATTCT950860.3772158844678267No Hit
CATACG950850.3772119173655775No Hit
ACACGA933790.37044404092843525No Hit
ATCCAC932170.36980137036406413No Hit
TATTGG930830.3692697786626708No Hit
AACCTT920850.36531061061796505No Hit
CACATT902470.3580190766839278No Hit
TGCATA877440.3480894197541698No Hit
ATCCGG872710.3462129803902962No Hit
CGAGCC870410.3453005468729793No Hit
ACGAAC864370.34290441711445996No Hit
ACCGCT863110.3424045622310602No Hit
ATTGAA859400.3409327672966055No Hit
TCAAGC855220.33927451855643814No Hit
AAGCAT838120.33249077371029906No Hit
CGAATT831300.3297852099763418No Hit
CGAGAG796760.31608283880759064No Hit
TAATGA790590.3136351367198316No Hit
GATCCT780920.3097989488448512No Hit
CTAGTT764100.30312628286168986No Hit
ATCAGC732870.2907370225374252No Hit
TCCACG720990.2860241050653707No Hit
GAGCCA710160.28172773332948264No Hit
GACCAT700150.27775666397802923No Hit
TGGCAT699160.27736392085535805No Hit
GTCTGC690820.27405535757952176No Hit
GGATCG685200.271825846115469No Hit
CTTAGC641390.2544459711617056No Hit
ATTCAT557880.22131670027860165No Hit
ATGGCC553910.21974176068566761No Hit
GGCTAC553400.21953943847095816No Hit
GCCAAT537250.21313256833849348No Hit
CTATGC532870.21139497755334205No Hit
AGCCTA512760.2034171349301925No Hit
CCTGCT511280.20283000379731028No Hit
CACCTA508230.201620037611303No Hit
CCGACT507580.20136217596510475No Hit
ACTTGA506660.20099720255817793No Hit
CCTTAT504290.20005699932511656No Hit
ATGCCG491110.1948283585606655No Hit
TTAGTA483730.1919006371007528No Hit
AGACGG476510.18903638927682742No Hit
TAGCTT473870.18798907428303752No Hit
CAGATC467780.18557310901327218No Hit
TCGTGG467430.18543426043455005No Hit
TCTCTG462400.18343880800320034No Hit
GAATAC458740.18198684857999162No Hit
CGATGT457940.1816694804000553No Hit
AAGTTG451200.1789956534840917No Hit
TTGAAT445650.17679391173578338No Hit
GCACAT440170.1746199397032195No Hit
TGACCA438330.17388999288936594No Hit
CATAAC437900.17371940749265014No Hit
TCTTCA432890.17173188926579885No Hit
CATTAA432750.17167634983431002No Hit
AGATGC431870.17132724483638004No Hit
CGTCAG409930.16262342250162612No Hit
GGTGGT399080.1583191165612396No Hit
TTACGT397870.1578390971890859No Hit
ACAGTG386120.15317775204627102No Hit
CAGTAG383380.15209076602998906No Hit
GGCGTT376090.14919874849031925No Hit
AAGCGC371580.14740958537592816No Hit
GACCGA370280.1468938620835316No Hit
GCGCAG366510.14539826453558163No Hit
GGCCAG362000.14360910142119057No Hit
ACCTGC361330.1433433055704939No Hit
AATGTA352430.1398125845687022No Hit
AGGTCA350410.13901122991436296No Hit
AACAAT340570.13510760130114607No Hit
ATGTGA331410.13147373564087506No Hit
TTAGGC325820.12925612548356993No Hit
GTCTCA324780.1288435468496527No Hit
GTACGC322040.12775656083337078No Hit
TTCGTT319360.12669337743058404No Hit
CAAGGA318560.1263760092506477No Hit
GTACTT313860.12451147119352174No Hit
ACGCTA312460.12395607687863316No Hit
TAGAGC311560.1235990376762048No Hit
AAGGCG308260.12228989393396743No Hit
TACTCG293390.11639081288940085No Hit
CCATGA293370.11638287868490244No Hit
TGTGTG292700.11611708283420574No Hit
AAGAGG288550.11447073540078602No Hit
CTCCGC287090.11389153847240223No Hit
GATCAG282700.11214998058500159No Hit
CGTCTC277860.11022990309638676No Hit
AGGCTT274780.1090080356036319No Hit
CAAGCT273610.10854388464047501No Hit
CCGGAA273360.1084447070842449No Hit
CAGCCT273000.10830189140327356No Hit
TCCAGA270140.10716730016000117No Hit
CGTACT268860.10665951107210303No Hit
AGGTGT267930.10629057056292705No Hit
GCTCAA267780.106231064029189No Hit
GGAGGC264290.10484654534421674No Hit
TGCGAC262600.10417610506410124No Hit
AGAACG261040.10355723711322538No Hit
CTTCAC260420.10331127677377472No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)