FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24166591
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTAG5635602.331979715302005No Hit
GCATAA5107122.1132976512905772No Hit
TACGGC4381901.8132056772094995No Hit
GTTACA4145061.7152026117378327No Hit
AGTAGG4090301.6925432304457009No Hit
ACAGCC3908331.6172450636500615No Hit
GGTACC3861341.5978008648385698No Hit
TATGGT3697281.5299137557299662No Hit
GAGTGA3486791.4428141726733406No Hit
GGACTA3336601.3806663918795994No Hit
CGAATT3300671.365798759121632No Hit
ACTAGC3231581.3372097040910735No Hit
GTAAGA3022211.250573570761387No Hit
TATTCT2898651.1994451348144222No Hit
CTGGAG2818321.1662050307385101No Hit
GAGTCG2773081.147484972125361No Hit
CCTCGG2765031.144153927212986No Hit
GAGCAC2717141.1243373134423469No Hit
ACGTAA2677141.1077855374802346No Hit
CAACGG2672451.105844841748677No Hit
TATAAG2541011.0514557059371759No Hit
GACCGA2535511.0491798367423855No Hit
AGTCAA2522951.0439825790902821No Hit
AATGTA2506331.0371053161780246No Hit
AATCGG2449401.013548001039948No Hit
AAGTTG2428841.0050403881954224No Hit
ATCCAC2414810.9992348527767114No Hit
GAGATA2410320.9973769159249644No Hit
ACTAAT2406330.9957258762727437No Hit
ATTCAT2375770.9830803194376899No Hit
TGTGTG2352190.9733230475080247No Hit
CACATT2340530.9684982048150689No Hit
GGATTC2217530.9176014937315735No Hit
GTCTGC2214360.916289765486576No Hit
TGATAA2178930.9016290299281351No Hit
ACCGCT2158690.8932538312913062No Hit
GCTTCT2154470.8915076189273033No Hit
TTCGTT2151210.8901586491863912No Hit
CGTTGC2122650.8783406811494431No Hit
CTATTA2055700.8506371461328575No Hit
CATTAA2049390.8480261034748342No Hit
CAGGAC2035200.842154360952275No Hit
ATCAGC2001820.8283419039118922No Hit
CTGTAT1981700.8200163606029497No Hit
ATTGAA1930880.798987329243086No Hit
AGGACT1912190.7912535119247891No Hit
TTCTGT1873760.7753513931691897No Hit
ATACCT1838910.7609306583621992No Hit
AAGCGC1831170.7577278897135306No Hit
GAAGAT1829630.7570906463389893No Hit
TCAATG1828630.7566768519399364No Hit
ATGGCC1767480.7313733244378572No Hit
CGTACT1703020.7047001374749131No Hit
GCACAT1646830.6814490301921359No Hit
CTAGTT1588580.6573455064473098No Hit
GACCAT1519360.6287026581448745No Hit
GATCCT1501230.621200565690047No Hit
ACACGA1427130.590538400720234No Hit
CGAGAG1406480.5819935463797935No Hit
TCTTCA1382850.5722155847301756No Hit
AGGTGT1356580.5613452058670584No Hit
GAGCCA1342410.5554817392324801No Hit
CGAGCC1333190.5516665548732133No Hit
CCTGCT1324310.5479920606096242No Hit
GGATCG1320000.5462086067497066No Hit
TATTGG1275940.52797682552744No Hit
CTTAGC1262140.5222664628205113No Hit
TCCACG1229440.5087353859714844No Hit
ATGTGA1209940.5006663951899546No Hit
TACTCG1171750.4848635870901279No Hit
TGCGAC1129400.46733939429024146No Hit
CGTCTC1122420.46445110938485284No Hit
CATAAC1108400.45864971191013243No Hit
TTAGTA1100510.45538487410160583No Hit
GGTAAT1064820.44061655199941113No Hit
ACCTGC1041870.43111997054114914No Hit
TCCAGA1027420.4251406414748361No Hit
GCGCAG1023530.42353098126252064No Hit
CTCCGC1003160.4151019893538149No Hit
AGAGGT1003000.4150357822499665No Hit
CTACCG992130.41053783713226244No Hit
GCTCAA985840.40793507036222026No Hit
TCTCTG978650.40495988863303056No Hit
CACCTA954600.39500813333581064No Hit
AGATGC954210.39484675352018No Hit
TCTCGC951510.3937295086427374No Hit
CAAGCT923810.3822674037889746No Hit
GTCTCA923000.3819322303257418No Hit
AAGAGG917750.3797598097307146No Hit
CGTCAG916900.37940808449151975No Hit
GCCAAT900870.37277496027470325No Hit
TTCACA893910.36989495125729566No Hit
CCTTAT888380.3676066682305336No Hit
TAGAGC885320.36634045736943205No Hit
AGTTCC853090.35300386388796No Hit
CCGGAA852940.3529417947281021No Hit
TTGAAT852190.35263144892881254No Hit
TCGTGG846780.3503928212299368No Hit
CGTGAA843160.3488948855053656No Hit
CAAGGA834460.3452948742336062No Hit
ACGCTA827990.34261762447173455No Hit
AGGTCA825660.3416534835219415No Hit
CCATGA813800.3367458819491752No Hit
CCAGCG793130.3281927517207537No Hit
GAGAAG776200.3211872125447896No Hit
AGAACG773610.3201154850512429No Hit
AGGCTT765730.31685478518670673No Hit
AAGGCG755590.3126589099803112No Hit
GTCGAG754480.31219959819736265No Hit
TTACGT742700.30732510017652054No Hit
GAATAC740520.3064230283865854No Hit
CTTCAC725500.30020783651281224No Hit
ACAGTG724850.29993887015342796No Hit
CTCGTC704600.29155953357260855No Hit
TGACCA689400.2852698587070059No Hit
CAGCCT688610.28494296113175416No Hit
GGCCGT683290.2827415749287932No Hit
CATACG677380.28029605003039115No Hit
GATCAG669110.2768739703502244No Hit
GTACGC664080.27479258452298877No Hit
TTAGGC634420.2625194426470825No Hit
GGAGGC629180.2603511599960458No Hit
CGATGT615810.2548187288807098No Hit
ACTTGA596130.24667525510735044No Hit
GGCGTT583760.24155661839106723No Hit
GGCAGG578260.23928074919627682No Hit
CCTGAC573320.2372366048649559No Hit
ATGCCG563350.23311107470639944No Hit
CAGTAG557840.23083106756761843No Hit
AGCCTA557320.23061589448011097No Hit
TTAGCG524650.21709723146305573No Hit
AAGCAT520510.21538412265097712No Hit
TACAGT519590.21500343180384857No Hit
CCGACT516560.21374963477471853No Hit
TAAGTC516220.21360894467904057No Hit
GGCCAG512660.2121358366184126No Hit
TGACAC503260.20824616926731618No Hit
GGTGGT498100.2061109901682037No Hit
AACTCT497450.20584202380881939No Hit
AACAAT493570.20423650154049447No Hit
TCAAGC484260.20038407568531286No Hit
CAGATC480190.1986999324811679No Hit
GCTGTC478180.19786820573907174No Hit
TGGCAT475550.19677992646956288No Hit
GCCATA469610.19432198773918918No Hit
TAGCTT456290.18881024634380578No Hit
CGCTTG454180.18793714016180435No Hit
TAATGA447050.18498678609655783No Hit
AACCTT434100.17962814862882398No Hit
TGCATA432590.17900331908625425No Hit
GACTAA429930.17790262598477377No Hit
AGTTAG429450.17770400467322842No Hit
ATCCGG422710.17491503042361248No Hit
CTGTTC413400.17106260456843086No Hit
GAACCG403700.16704879889761862No Hit
ACGAAC393480.16281982013929894No Hit
CTATGC384750.1592073950355679No Hit
CATGAG380660.15751497594344194No Hit
GTACTT379990.15723773369607652No Hit
TGTGAT377720.15629842041022668No Hit
AACATA364740.1509273691105212No Hit
CGAAGC363350.15035219489583782No Hit
AGTGCA357790.1480514980371042No Hit
GCGTAT355540.1471204606392354No Hit
TTCCAT352340.1457963185622664No Hit
CCGCTC340310.1408183719416611No Hit
TCGGAC334620.13846388181105063No Hit
ATTGCT325110.13452869707605844No Hit
CGACAA307040.12705143228517418No Hit
TGCGGA299810.12405969878002239No Hit
TACGAA296420.12265693576723337No Hit
GACTTG293470.12143624229002759No Hit
TCCTCC290830.12034382507652816No Hit
ATTCGA290300.12012451404503019No Hit
GACACG278220.11512587770447227No Hit
ACCAGG277950.115014153216728No Hit
CAGTCC273760.11328035468469674No Hit
TTCTAC271160.11220448924715945No Hit
AGACGG251850.1042141194014497No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)