FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407447593
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGTG47410831.1636055977387993No Hit
AAGCGCAC47135491.1568479188438843No Hit
GGTGCGGA45832531.1248693276732649No Hit
GGTGAGAT45291461.1115898284371506No Hit
CTATGGAG41599231.0209713031732157No Hit
CATAATAA38959600.9561867751664446No Hit
TGCCTGAC38318460.9404512545494409No Hit
AGCTGTGG38060400.9341176792766082No Hit
TGCATCAA35205300.8640448638016619No Hit
ATCATGGA34140670.8379156138492638No Hit
ATGCAACT33276730.8167119053271717No Hit
GACGCTCA33122070.8129160797374007No Hit
TAATGATT32020700.785885118727404No Hit
ACTAAGTG31661360.7770658250029225No Hit
GTTGGTCG30945110.7594868771258148No Hit
GCCTGTGA29709070.7291507057694165No Hit
CCAATCTA29047680.7129181887202853No Hit
CTAACATA29039990.7127294527912452No Hit
CCGGTCTT28915100.709664273314286No Hit
CGCATATA28687200.7040709159374026No Hit
CGAACTGC28524770.7000843909758967No Hit
ACCAGCGG28041210.6882163616070252No Hit
CATTAAGT27963090.6862990598155283No Hit
ATATTACA27346970.6711776059013312No Hit
GAGACCAT27151090.6663701164630516No Hit
GTATGCCT27014750.6630239192503955No Hit
CAGATTCA27002110.662713695304613No Hit
GCTCACGT26820400.6582539806536543No Hit
TCGTCTGG26745630.6564188980250032No Hit
AGAGTATT26383200.647523766326434No Hit
TCTGCGCA26183860.6426313579916031No Hit
ATTATCGT25802450.6332703995136867No Hit
TTGTACTC25753100.6320592008013163No Hit
TTCGGAAG25694540.6306219607487041No Hit
TGGCTTCC25632750.6291054466972885No Hit
CGAAGCAA25550900.6270965993901454No Hit
CGTGCCAC25317310.6213635921515924No Hit
CCTTACTG24714350.6065651245606941No Hit
AACTACCT24678800.6056926197131811No Hit
AAGACACT24561350.6028100404068407No Hit
CCAACAAT24485590.6009506601748412RNA PCR Primer, Index 36 (100% over 8bp)
TGTACGCC24400240.5988559122497994No Hit
GAGAGCGG24379740.5983527800592504No Hit
TTGAGAGT24365540.598004268980919No Hit
CTGTTCGC23916180.5869756113640853No Hit
GTCAGATG23654540.5805541720306592No Hit
AGACAGAG23620830.5797268263651272No Hit
ACCGTTCA23215050.5697677541562995No Hit
ACTCGGAA22990380.5642536707782195No Hit
CCGACCGC22810130.5598297889564413No Hit
TGCTGCCG22552280.5535013677206826No Hit
CGTTCGCG22259400.5463132040149272No Hit
GGCGCCAT22101400.5424354046926472No Hit
AGCATAGT21996210.5398537229792888No Hit
AACGAGAT21648180.5313120109657882No Hit
GCCGTCCT21623850.5307148789562244No Hit
AGCACAAC21573420.5294771737674739No Hit
TCTACCGT21535720.5285519013975375No Hit
GTCATTAG21423750.5258038179157927No Hit
CTGGCCTC21181190.5198506596650824No Hit
GTCAACGT21141130.5188674657356486No Hit
CATCCTCA21059120.5168546915431158No Hit
TGGCCTAT20917660.513382833997991No Hit
CTGATCCT20830270.5112380182842312No Hit
GGATCTCT20799840.5104911737691871No Hit
CGCGTGAA20756420.5094255152465706No Hit
TATAACGG20450620.5019202555456009No Hit
ATAGGAGG20376120.5000917995360449No Hit
TCGGCCAG19902130.48845864699954183No Hit
AACCTTCT19884230.4880193266965747No Hit
CCAGATTC19808360.48615724673086974No Hit
GGCACGTT19574900.4804274300866958No Hit
TACGTCAG19473760.477945147659763No Hit
GCTCCAGA19189720.47097394437178575No Hit
AGCGCTTC19161710.47028649399826006No Hit
GGTCAACT19108490.46898031374552746No Hit
GCCTCATT19098160.4687267842075582No Hit
CAAGCACT19076430.46819346408557627No Hit
TAGCTGGT19045490.4674341026233526No Hit
CCGTGGCG18972550.46564393374634566No Hit
GGATACTA18932700.46466589385398577No Hit
GATATATG18903090.4639391746265636No Hit
CGCTGAAG18860330.4628897145061795No Hit
GAGTGGCA18844480.4625007074222672No Hit
GTTGTGTG18621780.4570349738205473No Hit
AAGTTACG18578870.45598183224511035No Hit
AGGTGTAA18543770.4551203717627557No Hit
TATCTACT18423770.45217520772051784No Hit
CATCGAGC18337700.45006278881122264No Hit
GTGGCGAA18315630.4495211240577877No Hit
CAGAGGAT18254040.4480095186131091No Hit
AGTTCCGG18177550.44613222196651925No Hit
TCGAGCAA18116760.4446402509487889No Hit
CTACGGCC18074210.44359594486547865No Hit
CCAGCCAA17966150.4409438246454434No Hit
ACTTCTAT17943830.44039602413358714No Hit
AGCCATTG17638540.432903281379797No Hit
TGCCAGGT17584630.4315801664338216No Hit
GAGCCGTC17535650.43037804864391477No Hit
GAAGCGAT17497800.4294490948189256No Hit
AGGCCGAC17407660.4272367857625312No Hit
CTCTCTGC17225160.42275768211496095No Hit
AACTCTAC17026330.4178777907273096No Hit
ATTGAGTA16995220.4171142569493594No Hit
CGACAGTT16897320.414711493951567No Hit
CAGCGTCT16768160.41154151572077147No Hit
ACGCGACC16659660.40887859656591463No Hit
AGGAATAC16452520.4037947525683383No Hit
GATTGGAT16324560.4006542259779652No Hit
GTTCGGAC16212040.39789264382769346No Hit
TAGAGTAG16052030.39396551300770594No Hit
CTCAGAAC15792410.3875936506023242No Hit
ATAACGCG15659550.3843328631468931No Hit
TGCCTAGA15617560.3833023011624467No Hit
AGGATTGG15511190.38069165866933957No Hit
ACGTGCGC15471580.3797195091050642No Hit
ATGCTAGG15458910.3794085488682713No Hit
TAGTCGTG15357070.37690908631775866No Hit
TTCACTTG15336050.3763931917496933No Hit
GGAAGGCG15253230.37436053769987543No Hit
TAATTGAT15124140.37119227748143796No Hit
CTTCCTGT14978100.36760801284203437No Hit
GATTATCT14920730.3661999789995078No Hit
TCGGTAAT14823440.3638121872522634No Hit
CTTAGATT14795340.363122528005706No Hit
CTCTTAAT14765110.36238059209739887No Hit
AACGCTGG14747850.36195697933599036No Hit
TGTAGACG14639360.3592943056114704No Hit
CTGTAGCA14544240.35695977224732306No Hit
GGAGAACC14459630.3548831861672085No Hit
CGGCCAAG14384730.3530449129441783No Hit
GGATCAGA14372140.35273591615008015No Hit
GGCCTGCT14237180.3494235883239099No Hit
ACAGCACG14202240.34856605472694496No Hit
TCTCTATA14154570.347396088311166No Hit
TAACATTA14113000.34637583440086733No Hit
TGTACCTA14099990.3460565295326214No Hit
GGTTGCAG14033000.34441239170604204No Hit
CCTTAGCC14031390.34437287742180867No Hit
CAATATGG14018780.3440633897670369No Hit
TGATAAGG13772850.338027521492807No Hit
CGAACGAG13721870.3367763176355296No Hit
AGTTCGTC13716820.3366523753154188No Hit
AATTGCTA13702920.33631122714719286No Hit
CCGTACAC13600900.3338073468506169No Hit
CTCTATAG13551890.3326044927696996No Hit
CGCGCGCT13411980.32917067692678703No Hit
GATGTCCA13375240.3282689658691885No Hit
ACATTCCT13304820.3265406454370685TruSeq Adapter, Index 27 (100% over 8bp)
GCCACGAC13210750.3242318822582908No Hit
ATCTGTCT13178460.32343938770059194No Hit
AACTAGTC13010570.3193188577751642No Hit
ATCCTAAC12883600.31620262878813965No Hit
CAACGTAA12874160.3159709425501503No Hit
TGAGGCGG12853970.31547541870004375No Hit
TCGCGGAT12739560.3126674502161067No Hit
TATATAGC12680940.31122873758147346No Hit
AGATGCGA12659350.3106988534842075No Hit
TTCGTTCG12453410.30564446112705346No Hit
GTCCTCAA12445790.3054574432103713No Hit
CTCCTTCA12435050.30519385102859103No Hit
CTGCTCAG12357690.303295201942695No Hit
GAGGTGCG12193870.29927456216436654No Hit
CACTCTCG11685340.28679369324437265No Hit
CTCACCTT11606460.28485773874727494No Hit
AGGCGTGC11605820.28484203120571633No Hit
TCAGGTAC11363230.2788881366639955No Hit
TATTAGAG11361900.278855494429194No Hit
CGTCCGAA11194210.2747398731105033No Hit
ACCACCAA11152500.2737161831754888No Hit
GGCCAATA11079890.27193411349959795No Hit
GCGCTGGC10758370.2640430373090951No Hit
CGCTACAA10589550.25989968236234007No Hit
GCAACAGC9861570.24203284470010353No Hit
TAAGCAAC9648600.23680591481614177No Hit
TTGGAACG8900660.2184491982015464No Hit
ACATCCAG8779660.21547949112562312No Hit
TATAATCC7495580.18396427243098232No Hit
GTCCAGAG6986390.17146720510875618No Hit
GTCTGGCG5334990.130936839280825No Hit
ATATGGTT4926250.12090511969228887No Hit
CAACCGGT4895680.12015483915252874No Hit
ACGAGTTC4538660.11139248526619717No Hit
CTCACGCC4452450.10927663033218607No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)