FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20860045
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACCA2558541.226526596658828No Hit
CTATCCTG2503311.2000501437077438No Hit
CAGGCGCA2412431.1564836029835985No Hit
ATGTTGTG2202731.0559564948206008No Hit
TCACGCCG2162061.0364598925841244No Hit
TCTGACCG2107081.0101032859708596No Hit
ATCCTCCG2087831.000875117958758No Hit
GAGGTATA1685170.8078458124131562No Hit
CGCCGGTA1593760.7640251974528339No Hit
CGCAAGTG1548040.7421076991924035No Hit
CTTGCCGA1532950.7348737742416184No Hit
GTTACCAA1483890.7113551289079194No Hit
ACTAAGTG1437530.689130824022671No Hit
TTAAGGAC1393470.6680091054453622No Hit
CATCTCCG1371710.6575776802015528No Hit
GAGCTTAG1294490.6205595433758652No Hit
AAGGCCTG1258470.6032920830228314No Hit
AGAGTATT1183840.5675155542569539No Hit
ACACTGTG1127040.5402864663043632No Hit
AGCTCACG1110320.5322711432309949No Hit
TAGCGCTG1099340.5270074920739625No Hit
AGGATCTT1070370.5131196984474385No Hit
CATACGTT1059540.5079279550930978No Hit
AACGCGCC1018310.4881628970599057No Hit
GGATCTCT986800.47305746464113574No Hit
AAGCTCCA961340.4608523135975977No Hit
TCGTCGAC877680.42074693510967975No Hit
CTTGCTTG866160.41522441586295716No Hit
GCCTCATT861270.4128802214952077No Hit
TCAACTTA796670.38191192780264854No Hit
TATACTAT786590.37707972346176627No Hit
CTCTCTGC778770.37333093001477224No Hit
CGGACATC765340.3668927847471087No Hit
CTGCGCTT753220.36108263428961923No Hit
ACTGAGGC737280.3534412317902478No Hit
TTAGAGAT719780.3450519881428827No Hit
GTATCTAG719270.34480750161373097No Hit
GATCTTCC717900.34415074368248005No Hit
CGAACGAG705680.33829265468986286No Hit
TGTAGACG704010.3374920811532286No Hit
CTATGGAG700950.3360251619783179No Hit
CCTGGCCA693730.33256399974209067ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGTGAGAT688330.32997531884518944No Hit
GGTTGCAG677990.32501847431297487No Hit
TTACAATT656250.31459663677619104No Hit
TGTACCTA649560.31138954877614117No Hit
TATGCCGC647680.31048830431573854No Hit
GTCCATCC639440.3065381690212078No Hit
CTCGGCCT635320.3045631013739424No Hit
ATGCAACT617400.2959725158790405No Hit
TTCCTTAT615620.29511920995376567No Hit
TAACTATG610910.2928613049492463No Hit
GCACTCTC603950.28952478290435135No Hit
ATCATGGA595350.2854020688833605No Hit
CAACGTAA595110.2852870163990538No Hit
CTTATGTA594090.2847980433407502No Hit
GACTTATC579600.27785174960073195No Hit
TCCAGATC574410.2753637396275991No Hit
GTTATCCG567710.27215185777403644No Hit
CCACCAGG567440.2720224237291914No Hit
CCATTGAC558180.26758331537635704No Hit
AGCTGTGG549570.26345580750185343No Hit
GACGCTCA546420.2619457436453277No Hit
AATATTCG546340.26190739281722547No Hit
GCCATGCG543100.2603541842790847No Hit
ACACGAAT539680.25871468637771394No Hit
CGCGGTCG538120.25796684522972024No Hit
CGGTTGTT537780.25780385421028573No Hit
TTGATGAG522120.25029667960927215No Hit
TGCTCAGC517620.24813944552852116No Hit
ACAGCGGA512270.24557473389918383No Hit
GCGTACGA506870.24298605300228257No Hit
TAATGATT505150.2421615101980844No Hit
TACTGCAC502000.2406514463415587No Hit
CACTTGCT495340.23745873990204722No Hit
TGCATCAA494960.23727657346856154No Hit
CATTAAGT493400.23652873232056787No Hit
GGTGCGGA493050.23636094744762057No Hit
CATGACTA492040.23587676824282977No Hit
CCGGCTGA490170.2349803176359399No Hit
CTCTGAGA488800.23432355970468902No Hit
CTGCCTTA486780.23335520129510748No Hit
AAGCGCAC483590.2318259620245306No Hit
TAGGTCTC477850.22907429010819488No Hit
CAGAGTGC475940.2281586640872539No Hit
ACCGACAC472530.2265239600393959No Hit
ATTCGATC469310.2249803392082807No Hit
CAAGACGC467110.22392569143546912No Hit
GCAGTTAA464830.22283269283455526No Hit
GCCTGTGA464260.22255944318432677No Hit
TGGTTAGT463770.22232454436220053No Hit
CCATAAGA457030.21909348709458681No Hit
GTAATCTT456160.21867642183897493No Hit
CTGACTGG454980.2181107471244669No Hit
CGGTCGGA454340.21780394049964896No Hit
TTGTGTAC452770.2170513054981425No Hit
CATAATAA451260.2163274336177127No Hit
CCAAGACG446060.21383462979106707No Hit
ATTATCGT444320.21300049927984338No Hit
TCTATGAA443930.21281353899284494No Hit
CCAACAAT443090.2124108552977714RNA PCR Primer, Index 36 (100% over 8bp)
TGGCTTCC442890.21231497822751583No Hit
ACGATAGA442410.21208487325890235No Hit
TAGGTGAA440780.21130347513631922No Hit
ACTCGGAA440150.21100146236501405No Hit
TTATGACG439540.21070903730073448No Hit
CTCGTATC436150.20908392095990205No Hit
TTAGGATC433080.20761220793147858No Hit
TTGACCGA432060.20712323487317502No Hit
TACAGCCT428740.20553167550693202No Hit
GTTGCCTT427390.2048845052827067No Hit
GGTTAATG427160.2047742466519128No Hit
AGATGGAC425160.20381547594935678RNA PCR Primer, Index 16 (100% over 8bp)
AATGTAGG424100.2033073274770021No Hit
CTCATAGG419330.201020659351406No Hit
TCCTCCTA418040.20040225224825736No Hit
CCGAAGGT417370.20008106406290113No Hit
GAACGCGC416040.19944348154570138No Hit
CAGCTAAC413300.1981299656831996No Hit
GTGCCACG412440.19771769428110053No Hit
ACTGTCAT411220.1971328441525414No Hit
TGCCTGAC409540.19632747676239432No Hit
ATCGCTAA409170.19615010418242146No Hit
AACCGTTC407240.19522489045445493No Hit
CGCACCGA406890.19505710558150763No Hit
CGAGGAGT406090.1946735973004852No Hit
TCGGCAGC405870.19456813252320404No Hit
ATTGACCT404320.19382508522872313No Hit
TGAGCTCC399600.19156238637069095No Hit
AACAGTGA398380.1909775362421318No Hit
CTCCAATG397800.19069949273839054No Hit
ACCTAGGT395660.18967360808665562No Hit
CAATACAT395650.18966881423314286No Hit
CTCAATTC393050.18842241231982002No Hit
CCGGTCTT392950.18837447378469221No Hit
TGCTTCTT386220.18514821037059123No Hit
ACAGGCTG385840.18496604393710558No Hit
GTCACAAT383490.1838394883616023No Hit
GGCTGAGT382300.18326901979358146No Hit
TGGCCGCA381910.18308205950658302No Hit
GATTGAGG380890.18259308644827948No Hit
CATTCTTC378480.18143776775169948No Hit
ACGCATTA374100.17933805991310184No Hit
CGCATATA373730.17916068733312895No Hit
ATGGAGAG373520.17906001640936056No Hit
GGCTACGG372910.178767591345081No Hit
GCTCCAGA370170.17745407548257927No Hit
CCTGTGCG367060.17596318704010466No Hit
GCGCGAGG366220.17556050334503115No Hit
GTTCACTG363980.1744866801581684No Hit
AGCGTTAT362790.17391621159014758No Hit
TAATAGGC362100.17358543569776574No Hit
CCTGGTGC358130.17168227585319207No Hit
TGTCGGCT357230.17125082903704186No Hit
CGATGCTC356600.17094881626573674No Hit
ACCTCGCA356490.17089608387709615No Hit
CAGGCTAG355920.1706228342268677No Hit
CTAACATA350040.16780404836135301No Hit
GCACACCA349680.16763146963489292No Hit
CGACTTAC348760.16719043511171716No Hit
CGTTCGCG342200.16404566720733343No Hit
CTTCTGCT341870.16388747004141171Illumina Single End Adapter 1 (100% over 8bp)
AGATGACT341100.16351834332092763No Hit
GCTGACAA340530.16324509367069917No Hit
GGATATGC340200.16308689650477742No Hit
GGTCAACT337980.16202266102494026No Hit
GAGTGGCA337920.16199389790386357No Hit
AACGGCCG330930.15864299429843032No Hit
CAAGCACT330710.15853752952114916No Hit
TGATCCAT329990.158192372068229No Hit
CGTAGTTC329910.15815402124012676No Hit
AATCAGCT329730.1580677318768967No Hit
TTGCAGAA328670.15755958340454204No Hit
CTCTCGAA328440.1574493247737481No Hit
GCCGTCCT328300.15738221082456916No Hit
ACCAGGCC327340.1569220008873423No Hit
CTTCGACG327030.1567733914284461No Hit
CAGATTCA325310.15594884862424793No Hit
GAGACCAT321950.15433811384395385No Hit
CGAACTGC321610.1541751228245193No Hit
CGTGCCAC319540.15318279514737385No Hit
TCGCAACA319020.1529335147647093No Hit
GGTTGGCC318450.1526602651144808No Hit
CATCAGTG318050.1524685109739696No Hit
ACTGGTTA317950.15242057243884183No Hit
CGCTGAAG315580.15128442915631296No Hit
ACGCTATT315050.1510303549201356No Hit
AAGTGGAG314260.15065164049262597No Hit
GTATGCCT313640.1503544215748336No Hit
CGACTCTG312630.14987024237004282No Hit
TCGAGCAA309810.14851837567943887No Hit
TTCGGAAG309790.1485087879724133No Hit
ATCGCGTG308280.1477849160919835No Hit
AGACAGAG308130.14771300828929182No Hit
GGCGCCAT307970.14763630663308733No Hit
AACAGAAT307170.14725279835206492No Hit
GCATAACG307070.14720485981693712No Hit
CGAAGCAA304740.14608789194845936No Hit
CGTGTTAG304090.14577629147012866No Hit
GTTGGTCG302860.1451866474880567No Hit
TATACGGA300770.1441847321038857No Hit
AACTACCT299790.14371493445963324No Hit
CAAGAGAG299690.14366699592450544No Hit
AGTTACAT298580.14313487818458684No Hit
ATACATGT297160.14245415098577208No Hit
TGGTTCCA296780.14227198455228643No Hit
GGAACCTG295870.14183574388262346No Hit
AGAAGAGC294550.1412029552189365No Hit
AATTATTG292990.1404551140709428No Hit
TGTAGCAC292880.1404023816823022No Hit
TTCCGAGC292280.1401147504715354No Hit
GAGCGTTA292130.14004284266884373No Hit
CATGTATT291870.13991820247751144No Hit
TAGCCACC291580.13977918072564083No Hit
GATTACGC290890.139448404833259No Hit
CGTTGTCT289930.13898819489603212No Hit
TCCGGTCT289610.13883479158362313No Hit
TCTTAGTA288210.13816365209183393No Hit
TCCGGCGA283960.1361262643489024No Hit
CGTCGTGG283420.1358673962592123No Hit
GCGGAGTA280140.13429501230702043No Hit
CCGTTACT280120.13428542459999487No Hit
ATAATATG279880.13417037211568814No Hit
GCCAACTG279290.13388753475843412No Hit
GACGCAAG277510.13303422883315927No Hit
AGCACAAC275440.1320419011560138No Hit
GGACGTTG275370.13200834418142435No Hit
TAATTAGA275100.1318789101365793No Hit
TCGTCTGG274820.13174468223822144No Hit
ACGATTCT274680.13167756828904253No Hit
CCAATCTA274610.13164401131445305No Hit
ACTCTTGT274430.13155772195122303No Hit
ACGCCTCG274120.13140911249232684No Hit
GTCTCCAC273420.13107354274643224No Hit
CATATCTC270880.1298559039541861No Hit
CAAGGTCC269970.12941966328452312No Hit
CACACATG269580.12923270299752468ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CTATCGCT268710.12881563774191282No Hit
TTGACTCT266150.12758841124264114No Hit
GAAGCGAT266150.12758841124264114No Hit
GCGCAATC262690.12592973792721923No Hit
GTGTAGAT262260.12572360222616968Illumina Single End PCR Primer 1 (100% over 8bp)
GACGCCTC262140.12566607598401633No Hit
CATCCTCA261580.12539762018730066No Hit
TCTGCGCA261090.12516272136517442No Hit
TGGAGTTA260880.12506205044140606No Hit
TGTATATT260870.12505725658789327No Hit
ACCAGCGG260440.12485112088684372No Hit
GTCATTAG260360.12481277005874149No Hit
GCTCACGT259800.1245443142620258No Hit
TACGTCAG258360.12385399935618546No Hit
CGTACACT256680.12304863196603841No Hit
CGGAGCTG256230.12283290855796332No Hit
GCCTGGTC255550.12250692651909428No Hit
TATGAGGT255290.122382286327762No Hit
CACACCAC254920.12220491374778913No Hit
CGCGTGAA254680.12208986126348241No Hit
ATAGCCTA253680.1216104759122044No Hit
GGATACTA253660.12160088820517886No Hit
GTCGAAGC251550.12058938511398226No Hit
ATGCTAGG251500.12056541584641836No Hit
GCGACAAG251430.12053185887182889No Hit
TATAACGG251110.12037845555941994No Hit
AGCATAGT250560.12011479361621705No Hit
TCTCTATA250420.12004767966703811No Hit
CTGTAAGG250070.11987989479409081No Hit
TGTACGCC249520.11961623285088792No Hit
AGACTCAA247190.11849926498241016No Hit
CGACGCCT246460.11814931367597721No Hit
ATGGCGCT246400.11812055055490053No Hit
GTCAGATG245790.11782812549062094No Hit
GAAGGTGA245330.11760760822903307No Hit
TTCGACAA245200.11754528813336693No Hit
AATTAACA244700.11730559545772791No Hit
GATGTCCA244660.11728642004367679No Hit
GTAAGAGA244380.11715219214531895No Hit
AGTGTCTC244360.11714260443829339No Hit
CTGATCCT243120.11654816660270867No Hit
GTAATTCC242830.11640914485083806No Hit
GCATCGAA241630.11583388242930445No Hit
CTGTAGCA241290.11567089140986993No Hit
CAGATAGT240130.11511480440238744No Hit
GATACACC240080.11509083513482354No Hit
CACGCCGT239160.11464980061164777No Hit
TTAAGTGG238990.11456830510193051No Hit
AACCAACG238310.11424232306306147No Hit
TGCAATCG237580.11389237175662853No Hit
AGTGGCAA237390.11380128853988569No Hit
CCAGATTC237180.11370061761611731No Hit
AAGTTACG234420.11237751404659002No Hit
GAGCCGTC233660.11201318117961875No Hit
TCGTGAAG232870.11163446675210911No Hit
CATCGAGC232430.11142353719754679No Hit
ATCATCTC231320.1108914194576282Illumina PCR Primer Index 7 (100% over 8bp)
TGCTATAC230360.11043120952040132No Hit
TTATCGTC229760.11014357830963452No Hit
AGGTGTAA229560.11004770123937892No Hit
CCGCTGTA229420.1099805872902No Hit
AACTCTAC227850.10922795228869354No Hit
TCGGCCAG227620.10911769365789958No Hit
ATGAGACG227550.10908413668331012No Hit
GACTCGGT226410.1085376373828532No Hit
ATATTCGG225880.10828356314667585No Hit
CCTTGTAG225280.10799593193590905No Hit
CTTACAAG225140.10792881798673012No Hit
TTCGCATA225110.10791443642619179No Hit
ACGTGCGC225100.107909642572679No Hit
CCAGGATA224080.10742066951437546No Hit
GGATTGTG223730.10725288464142814No Hit
TGCTGCCG222780.10679746855771403No Hit
CTGGCCTC221300.10608797823782261No Hit
ACTTCTAT220960.10592498721838806No Hit
GCGGCTCC220850.10587225482974749No Hit
AGAGATCG220450.10568050068923629No Hit
TAAGTCCT219350.10515317680283048No Hit
TATTAGAG219240.10510044441418989No Hit
TTAGCAGT218080.10454435740670741No Hit
ACTACATA217860.10443889262942625No Hit
AGGCCGAC217310.10417523068622335No Hit
TGGCACAG216720.10389239332896934No Hit
ACTATTAC216690.10387801176843098No Hit
TATCTACT216580.10382527937979041No Hit
ATATTACA215840.10347053421984467No Hit
TGACGGAA215640.10337465714958909No Hit
GGATCAGA215520.10331713090743573No Hit
AGCGCTTC215370.10324522310474402No Hit
CCGAATAG215010.10307264437828392No Hit
CGTATGAC214300.10273228077887656No Hit
GTCAACGT211790.10152902354716875No Hit
CAACCGGT210310.10081953322727732No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)