FastQCFastQC Report
Thu 23 Mar 2023
EGAF00001836264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408545817
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACGAC49209281.2044984418479556No Hit
GCCACTGT43368531.0615340604503116No Hit
TGCTATAC42436991.0387327010620206No Hit
TCTGTGTT40180270.9834948328451495No Hit
TTAGGTCA40104520.9816406956383059No Hit
TATCACAT38540230.9433514772713975No Hit
TGCGCCTG37592280.9201484493476041No Hit
TTAAGTGG36777470.9002042970372647No Hit
CGTAGTTC35164630.860726717463858No Hit
GTGATGTC34459920.8434774893313863No Hit
ACGCCTCG32500230.7955100419006371No Hit
ACTGTCAT32008470.7834732034473382No Hit
TCTCATTC32003040.7833402930178575No Hit
AGCGTTAT31673990.7752861168077998No Hit
ACAGTGCC31627870.7741572348542735No Hit
CCACCAGG31529840.7717577487765589No Hit
GTATATCA31476150.7704435754876423No Hit
TATGCTTA30955060.7576888248986772No Hit
CTGATTAC30529120.7472630664579782No Hit
GACATCTA29786700.7290908084367929No Hit
CATGTATT29222330.715276690741396No Hit
CCTCCATC29122300.7128282505460091No Hit
CAGCACAA29092870.7121078907044592No Hit
TCCAGATC28295400.69258817059434No Hit
AGGACCAG26946220.6595642123536906No Hit
CCAAGACG26519330.6491152007070973No Hit
GACGCAAG25912640.6342652138817517No Hit
ATCTAACC25843620.6325758072808759No Hit
TCATCATG25817640.6319398932923109No Hit
GCGTCGCT25497750.624109926941193No Hit
CGACTTAC25405230.621845309457666No Hit
CTAGGCAC24901540.609516459692451No Hit
TCTCTGCC24797910.6069799020852538No Hit
GATGGTAC24688530.6043026013897482No Hit
CTTCGCAA24400200.5972451310155013No Hit
CACGCCGT24014620.5878072666694321No Hit
TGAATTGA23836080.5834371325848137No Hit
CTCTAGTT23641230.5786677776705764No Hit
AACAATAG23544740.5763059862634697No Hit
GCGCTCAT23511690.5754970194689327No Hit
GTAGTGAC23486110.5748708963039022RNA PCR Primer, Index 43 (100% over 8bp)
AGTGGCAA23477420.5746581906626154No Hit
ACGACGAT23450640.5740026950269814No Hit
ATTAGGCT23406660.5729261939793646No Hit
ACCTATAA22910190.5607740685789472No Hit
TTCTATGA22828870.5587835941543858No Hit
AGGTCTTG22347850.547009639312988No Hit
GCTAGTCT22297320.5457728135299938No Hit
GCGATCCA22146190.5420735956280761No Hit
CACCACGG22030250.5392357254266049No Hit
TGCGGATT21875230.5354412917658143No Hit
CACGATAC21760120.5326237375231772No Hit
TCGGAGCT21702450.5312121455400924No Hit
GCATCGAA21286300.5210260175054001No Hit
CGATCTAA21077470.5159144733086326No Hit
AAGGTGTT20881040.5111064446414342No Hit
GTTCACTG20837420.5100387553349983No Hit
CCTTGGTT20757130.5080734922810384No Hit
GGTCTCGC20646100.505355804438453No Hit
TTCGACAA20400860.49935305052945866No Hit
GAGTTCCT20261790.49594902595710577RNA PCR Primer, Index 1 (100% over 8bp)
AGTGCTCA20094840.49186258098439906No Hit
TATTGACC20040150.4905239306366463No Hit
CCTGTGCG19438650.4758009797466608No Hit
CTATCGCT19268850.4716447751562709No Hit
AAGATGGC19197570.4699000504024252No Hit
TTATACGT19194260.4698190313376774No Hit
CCTAACCT19121290.4680329403543985No Hit
TCCACAGG18970490.4643417998818967No Hit
ATTCCACA18839670.4611397110449426No Hit
TATGCCGC18767740.45937907620285345No Hit
GAGAATTC18673970.45708386239578114Illumina Small RNA Adapter 2 (100% over 8bp)
CTGTGTTG18658530.45670593660735No Hit
AAGCATCC18568550.454503490853267No Hit
TCTGATAT18447860.4515493546223238No Hit
ACAAGTGT18444910.45147714729875693No Hit
TAAGTCCT18247250.44663901184919No Hit
AGCAGGAA18202530.4455443977780343Illumina Paired End Adapter 2 (100% over 8bp)
CTCATAGG18176230.44490065113064176No Hit
TTCAGTAA18147770.444204034036163No Hit
CAACTCGA18026380.44123276386403437No Hit
TATGAGGT17926940.4387987651333608No Hit
CATTCTTC17822600.43624482881438975No Hit
GTCACAAT17647880.4319681970945256No Hit
GCGGCTCC17596410.4307083628762255No Hit
CAAGGTCC17588830.4305228267702469No Hit
TGACGACC17581690.4303480605701563No Hit
GTCGACCG17556000.4297192449286539No Hit
TGGCCGCA17535770.42922407402839713No Hit
GGCTACGG17465790.42751116945103856No Hit
GTAAGAGA17463850.4274636839568963No Hit
CGAGTTCT17448730.42709359082729276No Hit
TCTCTCAG17344160.42453402478478935ABI Solid3 Adapter A (100% over 8bp)
CATCAGTG17300480.4234648668548233No Hit
TTGACTCT17194720.42087617311230485No Hit
CGGTCGGA17193980.420858060088766No Hit
TGGTTAGT17098880.4185302917934416No Hit
CGGTATAT17043560.41717622089862194No Hit
TTGATGAG17014460.4164639384864881No Hit
AGAATGTA17006010.416257107339322No Hit
TGGCACAG17003080.41618538955693185No Hit
CCTATACC16965570.4152672550799853No Hit
ATACGTTA16914220.41401035810874554No Hit
TTCCGAGC16848450.4124005019490874No Hit
GAGCGTTA16846420.41235081351964986No Hit
ACTATTAC16793360.4110520607778001No Hit
GTGTAGAT16669660.40802424859975983Illumina Single End PCR Primer 1 (100% over 8bp)
CACACCAC16654150.4076446094172101No Hit
TACAAGAA16625970.40695484589920544No Hit
AATGTAGG16614860.4066829057755351No Hit
CGTATCCA16591950.40612213635759736No Hit
TGCCGTAG16534110.4047063832745105No Hit
GAGAAGCT16520170.4043651730743335No Hit
AGCAACTA16466580.4030534474913006No Hit
TGAGATAA16363470.4005296179546981No Hit
GCTTATAC16149760.3952986257108098No Hit
TACCTGCA16078090.393544354904018No Hit
GCACACCA16075390.39347826684516024No Hit
CCTTGTAG16074680.39346088813338653No Hit
GCACGTCC16074480.39345599272161924No Hit
TACCTCGC15790240.3864986335180125No Hit
CACCGGAG15737560.3852091820585205No Hit
ACAACCTT15621880.3823776758923467No Hit
GCTGACAA15604300.381947369198006No Hit
AGAGATCG15553980.38071568359736746No Hit
CTGCCTTA15424790.3775534923663164No Hit
CTTCTGCT15166000.3712190743100914Illumina Single End Adapter 1 (100% over 8bp)
AACAGAAT15083120.3691904156737456No Hit
CCAGGATA14944890.36580695183081513No Hit
TTGCAGAA14933900.36553794895420505No Hit
ACACGAAT14826400.3629066651293116No Hit
ATTAGTGC14530970.3556754076373275No Hit
TGTAGCAC14422160.3530120588653585No Hit
GCTACCTC14330180.35076065899360315No Hit
CACTTGCT14033600.34350125288395744No Hit
ATGTAATA13904510.34034150935879No Hit
ATGGAGAG13811100.33805510729289884No Hit
GCAAGCAC13693880.33518590645611723No Hit
AATATTCG13642330.3339241140731102No Hit
ACTTAAGG13641020.33389204912603476No Hit
AGTAACCG13589890.3326405371077389No Hit
ATAGCCTA13571250.3321842847310318No Hit
TCAATCGG13522840.33099935031277045No Hit
TGTGTCGA13445900.32911608540591175No Hit
AGTCTGAT13445210.32909919623531475No Hit
CGGTTGTT13292160.32535298238043153No Hit
CTCAATTC13146510.3217878987609363No Hit
TTGCTCTA13100590.3206639122191771No Hit
AATCAGCT12992070.3180076617942707No Hit
ACCAGGCC12927290.31642203792286044No Hit
ACTGGTTA12839340.314269280598215No Hit
CCTGGTGC12681270.3104001919079739No Hit
TCAACTTA12674830.3102425596490687No Hit
GTGTGTGT12505710.3061029994586874No Hit
CTTCGACG12349560.3022809067214119No Hit
CCATAAGA12308030.30126437446794374No Hit
GTCCGCGG12264930.3002094132321027No Hit
GGTTAATG12102450.2962323807123939No Hit
GACAAGGC11819920.28931687727939703No Hit
ACGATTCT11805750.2889700373556878No Hit
AATCCAAG11534460.28232965606401006No Hit
AGACTCAA11048960.27044604399902594No Hit
CGGCTTGA10874170.26616769888504327No Hit
TTCCTTAT10784260.2639669665250789No Hit
TGGTTCCA10096610.24713531701635316No Hit
CGACGCCT10044990.24587181123922755No Hit
CGCGGTCG10023130.2453367427330678No Hit
CCGTTACT9725930.23806216084694362No Hit
TAGGTGAA9551120.23378332619178427No Hit
GATTGAGG9220520.2256912105405304No Hit
ATACATGT9159650.22420129196916977No Hit
AACCGTTC8928580.2185453779838847No Hit
CCATGCGG8925770.21847659744855497No Hit
CATATCTC8916090.21823965951902036No Hit
AAGAACGT8185970.20036846932152044No Hit
TATACGGA8165080.1998571435624318No Hit
ACGATAGA8089010.1979951736967607No Hit
CTCGGCCT8054480.19714998085514604No Hit
GCCAACTG7939140.1943267968889766No Hit
CATGACTA7720340.18897121641561196No Hit
CGCCAGCC7674210.1878420896914972No Hit
AACGGCCG7444260.18221359001210874No Hit
ACCGACAC7443700.18219988285916044No Hit
GGACGTTG7007230.1715163809889161No Hit
AACGCGCC6967150.17053534047076047No Hit
CTGCGCTT6889200.16862735373447724No Hit
ATGAGACG6842710.16748941526918143No Hit
CTATTGGA6561710.160611361736204No Hit
GTGCCACG6557500.16050831331850351No Hit
TCTTAGTA6542270.16013552771242792No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)