FastQCFastQC Report
Thu 23 Mar 2023
EGAF00001836268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412686946
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACG61180161.4824835288102376No Hit
ATGTGCAT49156091.1911229680620912No Hit
ATCGCTAA47015191.1392458728268087No Hit
AGTGTCTC44844011.0866350495128092No Hit
TATCAAGA42517731.0302659294679992No Hit
ATCGCGTG38958670.9440247717455061No Hit
TACTGTTG38386430.930158571092772No Hit
TTACAGCG37928340.9190583896007217No Hit
CCACTACA36683410.8888919398967372No Hit
CCAGCGTG35857770.868885491716038No Hit
TCGCAACA35651630.8638904221603365No Hit
TGTGGAGC34430550.8343018923598325No Hit
GCAGCTGG33585620.8138280196534252No Hit
GGCCGCTC33286540.8065808798323366No Hit
CGTGATCC32886970.7968987223550318No Hit
GGTCCGTG32718480.7928159666092274No Hit
TTAAGGAC32287990.7823845729300097No Hit
CTTGCCGA32260770.7817249930653245No Hit
CAAGACGC32201390.7802861300100342No Hit
GTTACCAA32186380.7799224160582002No Hit
ACTCCGTT31688940.7678687273040131No Hit
GATTACGC31217130.7564360904209458No Hit
TCGATTGC31137310.7545019366810793No Hit
CATCTCCG30774590.7457127078596762No Hit
ATCGGCGC30288410.733931865147971No Hit
GCTGGAAT29658250.7186621793459879No Hit
TGTGAACA29209600.7077907426710802No Hit
TGATCCAT28680690.6949744904216089No Hit
ACTCTTGT27711570.6714913148718786No Hit
ACTCATAG27687500.6709080640510494No Hit
CTTAACAC27055270.6555882191631038No Hit
AGATGGAC26962540.6533412375006405RNA PCR Primer, Index 16 (100% over 8bp)
AGTGGATG26782890.6489880588566036No Hit
ACCTCGCA26727960.6476570257204113No Hit
GACTCGGT26720430.647474562958432No Hit
CGCGATTA26495450.6420229730261446No Hit
AGGCCAGA26357250.6386741876734817No Hit
GTCTTGTA26265510.6364511951390873No Hit
GGAATATC25677890.6222123149007965RNA PCR Primer, Index 37 (100% over 8bp)
CGTGTGGT25635010.6211732706466563No Hit
GTGGTACC25375780.6148917538089513No Hit
GAGGTATA25210100.6108770884165549No Hit
GGCTGAGT24907230.6035381114284143No Hit
GGCCTAAG24893390.6032027482642982No Hit
ATAGTTGC24407570.5914306288718907No Hit
TTATGCTA24401830.5912915403919755No Hit
TCTAGTGA23737180.5751861121383762No Hit
TAGGATCA23733020.5750853093376014No Hit
GCCTTAGG23685490.5739335888758643No Hit
ATTCTGGC23565720.5710313890083647No Hit
TGGAGTTA23353600.5658914154265494No Hit
TAATTAGA23315110.5649587472049576No Hit
CAGGCTAG23285630.5642444042802363No Hit
ATAGGTAT22896600.5548176462068175No Hit
TTCCGGTT22700910.5500757952251778No Hit
GCGGAAGT22645460.5487321617388886No Hit
ATAAGCCA22612460.5479325241365884No Hit
TGTGAGTC22565940.5468052774317703No Hit
CTGACTGG22490730.5449828306418009No Hit
GTGCGGTG22449670.5439878876129995No Hit
CGCGTCGG22424880.5433871901535747No Hit
TACACACA22414260.543129852234289No Hit
GATATTGT22251460.539184973396275No Hit
CGGATGCG22105940.5356588138845564No Hit
TTCACGGT22030360.5338274014608642No Hit
AGCGAAGA22030020.5338191627704163No Hit
GGACCGGC22017580.5335177236257916No Hit
TGAGCGTA21905050.5307909594019482No Hit
GGTAGCGA21534490.5218117560713927No Hit
GCGTACGA21436660.5194411940522102No Hit
TCGTGAAG21377890.5180171121768412No Hit
CCATCTCC21340380.5171081907688934No Hit
GACGCCTC21092410.5110995199736703No Hit
GTTGCCTT21054260.5101750904425263No Hit
CGCACCGA20568710.4984095135395923No Hit
ATTCGATC20534770.4975870983813479No Hit
CTTACAAG20175690.48888607201062284No Hit
CGAGGAGT20092440.486868804423002No Hit
ACCTAGGT19756130.47871952799786405No Hit
TTCGCATA19576030.47435544520470485No Hit
GATACACC19406970.4702588775366789No Hit
TGTCTTAA19243890.4663072138947666No Hit
TACTCAAT18891540.45776926513202576No Hit
TGCAGTGT18816620.4559538454603796No Hit
AATTAACA18804080.45564998317150546No Hit
GGAGTGCA18776990.45499355339434455No Hit
GGCCGTCA18765920.45472531132593663No Hit
CGCAAGTG18587120.45039272940801967No Hit
AGTTACAT18186100.44067543633909856No Hit
TAACCTCT18035310.4370215771254369No Hit
ACAGGCTG17776530.4307509644368543No Hit
TCTGACCG17662030.42797646427129776No Hit
TACTGCAC17595940.4263750082368731No Hit
CCTACTCG17499090.42402819303133465No Hit
TCGGATTG17456160.4229879372050697No Hit
GGTGTACG17366200.42080807663831454No Hit
TAGATCCG17315660.41958341953467077No Hit
GGACACAT16894570.40937980141489627No Hit
CAGTGGTC16730950.4054150527940372No Hit
TGACGGAA16669830.4039340270288075No Hit
CGCTTACC16651120.40348065673974576No Hit
ACGTGGTA16276790.39441010087098805No Hit
CGATGCTC16209830.3927875634815936No Hit
CTGAACTA16156120.3914860927052439No Hit
CAGGCGCA16093340.3899648427454742No Hit
ATAACTGA15948200.38644789118190326No Hit
ATAATATG15848310.3840274123911833No Hit
GGCTCTTA15790170.3826185963245855No Hit
GATGGCGT15726360.38107238797904697No Hit
GACTTATC15656970.3793909681843922No Hit
TAACGGTC15642180.3790325851499068No Hit
CACTGCTT15624880.3786133811947616Clontech Universal Primer Mix Long (100% over 8bp)
AGGTTATC15481870.37514804260370277No Hit
CGTAATGT15307000.370910690254787No Hit
CCGACACA15284170.37035748642265026No Hit
GCCATGCG15141160.36689214783159146No Hit
GTTCAGGA15094830.36576950510084705No Hit
GAGTATAA14980430.36299742807954No Hit
CCGAAGGT14757780.35760229740826355No Hit
ACACTAGC14743160.3572480337190021No Hit
GCGAGATA14714950.3565644647262479No Hit
TAATAGGC14706140.3563509857178763No Hit
AATCTGTA14699860.35619881225901434No Hit
AATCGCGG14642570.3548105929185364No Hit
AACCAACG14637310.3546831355310182No Hit
GACCTGTG14580650.3533101819993114No Hit
AAGCAGGA14526880.3520072573364121No Hit
AGCAAGCT14516550.35175694653544964No Hit
ACTACATA14492640.3511775727454195No Hit
GGTTGGCC14311240.3467819890770182No Hit
TGTCCTGC14042180.34026227715959784No Hit
TCGGCAGC13863170.3359246066387571No Hit
TCACGCCG13812690.3347014034216629No Hit
AGAGCCGC13789420.3341375377548288No Hit
TTAGAGAT13771090.33369337541391486No Hit
AACGGATA13664980.33112217705088254No Hit
TAGGCATT13522150.32766120011947264No Hit
TCGCACGC12893190.31242059204848216No Hit
GCATCCGT12834870.3110074143222354No Hit
TCATTCAA12732660.3085307185849295No Hit
TCCAACCA12668170.3069680328584951No Hit
CCGGTAGG12600310.3053236871708561No Hit
GAACAAGT12430780.30121573072485797No Hit
GTGTCGGC11924170.28893983964300146No Hit
GGAAGAAT11901400.28838808969741436No Hit
TCCGACTT11778140.285401322095611No Hit
TCCTTACA11762070.2850119228147333No Hit
AAGTGAGA11523230.27922448508947995No Hit
GTTGCAAC11400170.2762425637761753No Hit
GCGCGAGG11208520.27159860782221107No Hit
ATATACAA11184840.2710248072639545No Hit
CGAGCCTT10992640.2663675240166186No Hit
GACCACTT10971190.2658477595751235ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
ATTAATCA10904470.2642310377319277No Hit
GCACCACT10767880.26092126500168No Hit
CTTGCGAT10537430.2553371290789508No Hit
ATCCTCCG10321730.25011040693300723No Hit
GAGTGAAC10200870.2471817947931893No Hit
ATCATCTC10021360.2428320085511016Illumina PCR Primer Index 7 (100% over 8bp)
GTTATCCG9991610.24211112313690678No Hit
TTACAATT9950640.24111836093792996No Hit
CGTCGTGG9857670.23886556372926804No Hit
ACAGCGGA9850260.23868600874038795No Hit
TAATACCA9831350.23822779216282744No Hit
CGGCACTC9807910.23765980715076945No Hit
TACTCCGG9786690.23714561594104797No Hit
ATGGTCAA9713990.2353839900717383No Hit
CTATCCTG9422540.2283217361568786No Hit
AGCTTCAC9045170.21917751670293928No Hit
TGGCTGTG8885860.2153172055992292No Hit
CTGGATGC8679550.2103180166983038No Hit
CTAATGAT8643830.20945246957242017No Hit
ATTGACCT7951170.19266831861456554No Hit
GTAGCTTC7943570.19248415965161156No Hit
CGAGAGGA7934570.19226607666916606No Hit
GCATGTAT7794180.1888642244574414No Hit
CGCCTCAT7507650.1819211892396519No Hit
GGTAATTA7501880.18178137381646184No Hit
GGCACTCC7436580.18019906062160734No Hit
GGCGGAAC6618910.1603857370375849No Hit
GTACTATA6339750.15362128755097576No Hit
TCACCGAG6181580.14978860029170876No Hit
GGATTGTG6103790.14790363638010492No Hit
CTTATGTA5979290.14488682178960902No Hit
TTAATACT5181580.12555715779776566No Hit
CGGAGAGA4651350.11270892004420223No Hit
TCTATGAA4483670.10864579176681785No Hit
GGCGTTGA4464330.10817715566898499No Hit
TTAACCAG4253130.1030594750142642No Hit
TTCTTGCC4223510.1023417396875936No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)