FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001836270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26800960
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACGAC1733790.6469133941470753No Hit
TAGTCGTG1664760.6211568540828388No Hit
TCTCCACG1578200.5888595035401717No Hit
CACTCTCG1479220.5519279906391412No Hit
TGCTATAC1465500.5468087710290974No Hit
TCGTCGAC1393230.519843319045288No Hit
TTCGTTCG1368670.5106794681981541No Hit
CATACGTT1367660.5103026160256946No Hit
ACACTGTG1356130.506000531324251No Hit
TATCACAT1355110.5056199479421633No Hit
CAGCTAAC1333590.4975903848220362No Hit
GACATCTA1241260.46314012632383317No Hit
CGTAGTTC1240420.462826704715055No Hit
AGCTCACG1224610.456927662292694No Hit
CATAATAA1222060.45597620383747445No Hit
GAGCTTAG1219450.4550023581244851No Hit
CGCCGGTA1203230.44895033610736335No Hit
GCCACTGT1199440.44753620765823315No Hit
TTAAGTGG1198080.4470287631487827No Hit
GTATATCA1182570.4412416570152711No Hit
TCTCATTC1177220.4392454598641243No Hit
CTTCCTGT1171740.4372007569878094No Hit
CAGCGTCT1135350.42362288515038266No Hit
TATACTAT1118830.41745892684441155No Hit
CAATATGG1096560.40914952300216106No Hit
AGTGTCTC1071490.39979538046398344No Hit
CCTCCATC1069080.3988961589435602No Hit
TGCGCCTG1068130.3985416940288706No Hit
CCGACCGC1057940.3947395914176209No Hit
AGGATCTT1050210.39185536637493584No Hit
TTAGGTCA1044160.38959798454980715No Hit
TATCAAGA1033180.3855011163779208No Hit
TGCCTAGA1025090.3824825677886165No Hit
AATTGCTA1019230.3802960789464258No Hit
GTGGCGAA1013070.3779976538153857No Hit
CTTGCTTG1006250.3754529688488771No Hit
GCCTACAT993120.37055389060690364No Hit
ATCTAACC988980.3690091698207825No Hit
TCAGGTAC988710.3689084271608181No Hit
TATGCTTA981300.36614360082623904No Hit
AGGACCAG981280.36613613840698245No Hit
CGGACATC979180.365352584385037No Hit
AAGGCCTG968650.36142362064642464No Hit
TCGCAACA949530.3542895478370924No Hit
CGCGCGCT933360.3482561818681122No Hit
CAAGAGAG930990.3473718851862023No Hit
TTGTCACA899260.3355327570355689No Hit
CCACTACA894970.3339320681050231No Hit
AGTGGCAA892860.3331447828734493No Hit
TCCTGGAA892360.3329582223920337No Hit
CTCTCGAA882860.3294135732451375No Hit
ACCTATAA882590.3293128305851731No Hit
CACAGGCA879930.3283203288240421No Hit
GATTGGAT869800.3245406134705623No Hit
ACTGAGGC863050.32202204697145176No Hit
GTCCAGAG832980.31080229961911815No Hit
TGTTGCGT813860.30366822680978595No Hit
ATGAATTG811210.3026794562582833No Hit
CACGGCAA791810.29544090957935837No Hit
TTCTATGA782540.2919820782539133No Hit
GCATCGAA779960.29101942616980886No Hit
CACGATAC779890.29099330770241066No Hit
ATATTACA776770.28982917029837735No Hit
TATAATCC774070.2888217436987332No Hit
ATTCCACA768530.2867546535646484No Hit
CATCCTCA757100.282489880959488No Hit
CTCATAGG753610.2811876887992072No Hit
GGTCCGTG743880.2775572218308598No Hit
GATTACGC740000.2761095124950748No Hit
GCGCAATC725070.2705388165200052No Hit
TTACAGCG715040.2667964132628085No Hit
TTATCGTC714050.2664270235096056No Hit
ACATTCCT713950.2663897114133225TruSeq Adapter, Index 27 (100% over 8bp)
AGATGGAC713750.26631508722075625RNA PCR Primer, Index 16 (100% over 8bp)
AAGCTCCA713430.2661956885126503No Hit
ATAGGAGG710410.26506886320490014No Hit
GTTACCAA710350.26504647594713027No Hit
GTATCTAG698390.2605839492316693No Hit
TGATCCAT695030.2593302627965565No Hit
GTAAGAGA689800.2573788401609494No Hit
TAGCGCTG680140.2537744916600002No Hit
CATTAAGT673140.251162644920182No Hit
TCGTCTGG670890.2503231227538118No Hit
TTGCGTCG668100.2492821152675128No Hit
CATCTCCG667310.24898734970687617No Hit
TTGACCGA653530.2438457428390625No Hit
GTTAAGCC653150.24370395687318666No Hit
TAGGTCTC651360.24303607034971883No Hit
TGTGAACA646370.24117419674519122No Hit
AGGTCTTG639440.23858846847277113No Hit
GAGCGTTA636650.23754746098647214No Hit
AATGCGAA634050.23657734648311105No Hit
CCTTGGTT627230.23403266151660237No Hit
CAGAGGAT624460.23299911644956003No Hit
ACTCATAG622850.2323983916994018No Hit
GCCTGTGA622630.23231630508757894No Hit
ACACGAAT620630.23157006316191658No Hit
TACTGTTG617080.2302454837438659No Hit
CATTGTGA616660.23008877293947677No Hit
TTAAGGAC616360.22997683665062743No Hit
TTGCAGAA615590.22968953350924742No Hit
CCAGCGTG614990.22946566093154874No Hit
ATATGGTT613760.2290067221472664No Hit
CTCAATTC612630.22858509545926714No Hit
GTCCTCAA609570.22744334531300373No Hit
ATACCGAA609300.22734260265303927No Hit
CTTGCCGA608790.2271523109619954No Hit
AGGCCAGA608130.22690605112652681No Hit
ACGAGTTC603490.22517476985899013No Hit
ACGCATTA601010.2242494298711688No Hit
CTGCTCAG600750.2241524184208327No Hit
CGCGATTA598870.22345095101071005No Hit
CTCACGCC598430.22328677778706432No Hit
AACGCTGG596350.2225106861843755No Hit
TCTCTGCC594980.22199951046529676No Hit
GCGCTCAT591340.22064135016059125No Hit
CTAGGCAC588890.2197272038016549No Hit
TTAGGATC587820.2193279643714255No Hit
AGTGCTCA587280.21912647905149665No Hit
GCGTCGCT584910.21824218236958676No Hit
ATCGCTAA582260.21725341181808414No Hit
TGCTTCTT575920.21488782491373443No Hit
CGCTGAAG574650.21441396129093884No Hit
CTGTTCGC574390.21431694984060273No Hit
GGAACCTG572600.2136490633171349No Hit
CTTCTGCT570150.2127349169581985Illumina Single End Adapter 1 (100% over 8bp)
AGCTGTGG562580.20991039126956645No Hit
GCAGCTGG561340.2094477212756558No Hit
TCTACCGT554800.20700751017873986No Hit
GTTAATGG551870.20591426575764452No Hit
GCGGCTCC550760.20550010148890188No Hit
GCTTATAC543310.2027203503158096No Hit
AATTATTG539440.20127637218965289No Hit
CAACTCGA537070.200392075507743No Hit
GGTCGTAT533780.19916450754002843No Hit
GGCCTAAG528230.19709368619631537No Hit
CGTGATCC528170.1970712989385455No Hit
GAGGTATA527410.19678772700679378No Hit
GGTCTCGC524500.19570194500495505No Hit
TAAGCAAC524000.19551538452353945No Hit
GTTCACTG523480.19532136162286723No Hit
TCTCTATA522510.19495943428892099No Hit
GGTTAATG521960.19475421775936383No Hit
TCAATCGG518310.19339232624503003No Hit
GCTGGAAT517040.19291846262223442No Hit
TCAACTTA516840.19284383842966818No Hit
CACACCAC513070.19143717239979463No Hit
GGTTCAAT510060.1903140783016728No Hit
GTGAGTCA509370.19005662483731928No Hit
CTAACATA504210.18813132066911037No Hit
GGTGAGAT500170.1866239119792724No Hit
GTAATCTT500100.1865977935118742No Hit
GCAAGCAC493900.18428444354232087No Hit
AATTAACA491940.18355312645517177No Hit
GCTCACGT490300.18294120807612863No Hit
GCCTTAGG489780.1827471851754564No Hit
TTGTGTAC485720.18123231406636178No Hit
CGTGTGGT482790.18013906964526644No Hit
GTCGACCG479920.17906821248194094No Hit
TTCAGTAA478120.1783965947488448No Hit
GCTAGTCT473830.17679590581829904No Hit
TCTAGTGA473030.1764974090480341No Hit
GAGCCGTC472510.17630338614736188No Hit
ATGTCAAC468150.17467657874941794No Hit
ATAGTTGC467450.1744153940754361No Hit
TCTCTCAG464010.17313185796329683ABI Solid3 Adapter A (100% over 8bp)
TGTATATT458930.1712364034721144No Hit
GGTCAACT458630.17112446718326507No Hit
AGATGACT454570.16960959607417048No Hit
TGAGCTCC453150.16907976430695018No Hit
ACTCCGTT452260.16874768665003045No Hit
GTCTCCAC452120.1686954497152341No Hit
GGAATATC449190.16760220529413872RNA PCR Primer, Index 37 (100% over 8bp)
GCCTGGTC448670.1674081823934665No Hit
TTATGACG448350.16728878368536051No Hit
AGCAACTA446050.1664306054708488No Hit
GCAACAGC443870.16561720177187683No Hit
TGTCTTAA443610.16552019032154072No Hit
GTTCGGAC442390.1650649827468867No Hit
GTCTGGCG441890.1648784222654711No Hit
TGCTCAGC439790.1640948682435256No Hit
GTCTTGTA439690.1640575561472425No Hit
ATGTGCAT439300.16391203897173834No Hit
CCAGGATA437720.16332250785046507No Hit
ATCGGCGC436840.16299416140317363No Hit
GGCCAATA433890.16189345456282161No Hit
AAGACACT429970.1604308203885234No Hit
TGCATCAA429120.16011366757011689No Hit
ATGTAATA427760.1596062230606665No Hit
CTCACCTT427270.1594233937888792No Hit
TACACACA427260.1594196625792509No Hit
GGCGCCAT426280.15905400403567635No Hit
TCATTGCG424380.1583450742062971No Hit
CCGCTGTA424220.15828537485224412No Hit
CCATCTCC423700.1580913519515719No Hit
TATGAGGT422160.15751674566881188No Hit
TCCGGCGA421660.15733018518739628No Hit
TAGAGTAG421590.1573040667199981No Hit
TACCTGCA421480.15726302341408666No Hit
ATAATATG418710.1562294783470443No Hit
TTCCTTAT417680.15584516375532817No Hit
TCGGTAAT415410.15499817916970138No Hit
CCTATACC413270.15419970030924265No Hit
AGCGAAGA411000.1533527157236159No Hit
GTTCAGGA410770.1532668979021647No Hit
GGACCGGC406050.15150576695760154No Hit
TGGCACAG404730.15101324728666435No Hit
GGCCTGCT404070.15076698745119577No Hit
TTGGAACG403220.15044983463278927No Hit
CTGTCATT402180.15006178883144483No Hit
GAGAGCGG401490.1498043353670913No Hit
CACCGGAG400650.14949091375831314No Hit
CGAGTTCT397730.14840140054684609No Hit
GATATTGT394040.14702458419399903No Hit
CGCAAGTG391010.14589402767662055No Hit
AGTAACCG388130.14481943930366672No Hit
TGGCCGCA386530.14422244576313684No Hit
GGCTGAGT385400.14380081907513761No Hit
GCGGAAGT384230.14336426754862513No Hit
CCGTGGCG383140.14295756569913914No Hit
ATCGCGTG382190.14260310078444952No Hit
AACAGTGA381310.14227475433715805No Hit
TCGAGCAA379450.14158074934629208No Hit
ATGCTAGG378580.14125613410862894No Hit
TGGCCTAT378510.14123001564123075No Hit
ATACATGT377650.14090913161319596No Hit
CGCGGTCG376970.14065540935847073No Hit
GTGGTCGT375780.14021139541270164No Hit
GACGCTCA375750.1402002017838167No Hit
CGAAGCAA372030.1388121918020847No Hit
ATCTCAGT369190.13775252826764414No Hit
AAGCAGGA367880.1372637398063353No Hit
ATTCTGGC367380.1370771793249197No Hit
TCCGTATG367340.13706225448640647No Hit
AGAACACA366850.13687942521461918No Hit
TCTGACCG365740.13646526094587658No Hit
TGTGAGTC365650.13643168005922174No Hit
TCGCGGAT365260.13628616288371762No Hit
GTCAGATG362590.13528992991295835No Hit
CACACATG361170.13476009814573806ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
GGAGAACC360160.13438324597327858No Hit
AGTCTGAT359220.13403251226821727No Hit
AGAGATCG356010.13283479397752915No Hit
ATACTTAG355570.13267062075388344No Hit
ATGCAACT354030.13209601447112343No Hit
ATTCGATC353170.1317751304430886No Hit
CTCCAATG352760.13162215084832782No Hit
AATCCAAG351340.13109231908110752No Hit
GGAAGGCG350570.13080501593972754No Hit
TTCCGGTT350150.13064830513533846No Hit
TACTCAAT350100.13062964908719688No Hit
TGTGGAGC347640.12971177151863217No Hit
CTCTGAGA346880.12942819958688048No Hit
GCGTACGA345510.12891702386780174No Hit
TCCGCGAT344760.12863718314567835No Hit
CGACTCTG342600.127831241865963No Hit
TGAGCGTA342450.12777527372153835No Hit
GGCTACGG341700.12749543299941493No Hit
CGAGGAGT340670.12711111840769884No Hit
CTCCTTCA339600.12671187897746947No Hit
GCACTCTC339190.12655889938270867No Hit
TATACGGA338950.1264693503516292No Hit
ACCTCGCA337880.12607011092139983No Hit
ATTGCCAG337110.12578280778001982No Hit
ACGCTATT335970.12535744988239225No Hit
CAAGACGC334970.12498432891956109No Hit
CGGAGCTG334620.12485373658257018No Hit
GCGCTGGC333880.1245776270700751No Hit
CGCCAGCC333530.12444703473308419No Hit
ACCATAAG332130.12392466538512055No Hit
GTGTAGAT331050.12352169474526287Illumina Single End PCR Primer 1 (100% over 8bp)
CGTGTTAG330620.12336125273124546No Hit
CCAGATTC330540.12333140305421895No Hit
TGCCGTAG329130.122805302496627No Hit
TGAGGCGG328670.12263366685372463No Hit
AGATGCGA326680.1218911561376906No Hit
AGCGCTTC326150.12169340202739007No Hit
ATAGGTAT325810.12156654090002747No Hit
GACTTATC323850.12083522381287834No Hit
CCGGTCTT323090.12055165188112665No Hit
ATGCGGCA322750.12042479075376404No Hit
TTGCTCTA322480.12032404809379962No Hit
CCATGCGG322410.12029792962640144No Hit
AGTCTACC322310.12026061753011832No Hit
TATCTACT320060.11942109536374818No Hit
TCGATTGC319320.11914498585125308No Hit
CAGTACCG318560.11886141391950139No Hit
GGCCGCTC318010.11865619738994425No Hit
ACGATTCT317530.11847709932778527No Hit
AACTAGTC317080.11830919489451125No Hit
CGCTACAA314240.11724953136007067No Hit
ACCTAGGT313810.11708908934605328No Hit
GCGAGATA312650.1166562690291691No Hit
GTAATTCC311280.11614509331009039No Hit
GCTACCTC311130.11608912516566572No Hit
CAGGCGCA309720.11556302460807373No Hit
CATGACTA307910.1148876756653493No Hit
ACCTGAAC305880.11413024011080199No Hit
ACTCTTGT304870.1137533879383425No Hit
GTCGAAGC304770.11371607584205938No Hit
TCGGATTG304560.11363772043986485No Hit
AGGTGTAA302250.11277581101572481No Hit
GATGTCCA302020.11268999319427363No Hit
CTGTAGCA301880.11263775625947726No Hit
ATAGCCTA301050.11232806586032738No Hit
TAGCTGGT300980.11230194739292922No Hit
TCAAGGTT300330.11205941876708894No Hit
TGTAGCAC299820.11186912707604503No Hit
CTGAACTA298040.11120497176220552No Hit
ACGCCTCG297470.11099229281339175No Hit
CGTTCGCG297000.11081692596086111No Hit
CTTCTAGA296420.11060051580241903No Hit
CTTAACAC296360.11057812854464914No Hit
ATCATGGA296260.11054081644836602No Hit
CGCGTGAA295730.11034306233806551No Hit
CAGCACAA295310.1101863515336764No Hit
CATTCCAG293280.10942891597912911No Hit
TGATAAGG293070.10935056057693456No Hit
CTTAGATT291250.1086714804245818No Hit
GTGATGTC290090.10823866010769764No Hit
GCTTCTCA289970.1081938855921579No Hit
AATCTGTA289200.10790658245077789No Hit
ACCAGGCC288550.10766405382493761No Hit
GCCATCGC287200.10716034052511551No Hit
CAATTAAG285780.10663050875789523No Hit
CTATGGAG285180.10640663618019654No Hit
AACTTGAA284440.10613052666770145No Hit
TACTGCAC284380.10610813940993158No Hit
TCACGCCG284320.10608575215216172No Hit
TCCGGTCT284310.1060820209425334No Hit
TCTGTGTT282970.10558203885233962No Hit
CTCGGCCT280630.10470893579931466No Hit
ACCGACAC279070.104126867097298No Hit
CAGAGTGC279010.10410447983952814No Hit
GACGATGT278960.10408582379138656No Hit
AGAACGGT277340.10348136783160007No Hit
CCACCAGG277330.10347763662197176No Hit
GGACGTTG277270.10345524936420188No Hit
ATTGAGTA275570.10282094372738887No Hit
ATTAATCA273460.10203365849581507No Hit
CGGATGCG272700.10175008656406338No Hit
GTGCCACG272190.10155979487301947No Hit
GTGTTAAG270940.10109339366948049No Hit
CCACATAT270300.10085459625326854No Hit
AACGCGCC270140.10079489689921554No Hit
AGTGGATG269700.10063072367556983No Hit
CGATGCTC268830.1003061084379067No Hit
TGCCTGAC268470.10017178489128747No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)