FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408697916
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGTG58325741.4271112652309192No Hit
TTCGTTCG47816991.1699837001370959No Hit
CACTCTCG46236791.1313194462190506No Hit
CGCGCGCT43882911.073724829074No Hit
CTCACCTT43102391.0546271050719036No Hit
CCGACCGC42864641.048809850060503No Hit
TCTCTATA41497291.0153535992094465No Hit
TCGGTAAT41128881.0063393619066068No Hit
TCAGGTAC40948651.0019295033547468No Hit
CAGCGTCT39948340.9774539687156124No Hit
CTTCCTGT39883790.9758745625705614No Hit
AATTGCTA38424330.9401645688841732No Hit
CATAATAA37264230.9117793005824869No Hit
TCCTGGAA36878910.9023513102523381No Hit
GTGGCGAA35383640.8657651192916775No Hit
TTGTCACA34395640.8415907851118086No Hit
CCGTGGCG33969870.8311730662213604No Hit
GTCCAGAG33321210.8153016860501926No Hit
TGAGGCGG33298610.8147487103898029No Hit
GGAAGGCG33217090.812754083140468No Hit
GCCTGTGA32652670.7989438830414786No Hit
AGCTGTGG32621610.7981839085276863No Hit
CAATATGG32545750.7963277698729445No Hit
CACGGCAA32542700.7962531426267414No Hit
GACGCTCA32303910.790410440947783No Hit
CATTAAGT31343320.7669067732657585No Hit
TGCCTAGA30557830.7476874435542754No Hit
GATTGGAT29733390.7275150872068552No Hit
TATAATCC29648260.7254321306595554No Hit
CGCTGAAG29112610.7123258734698319No Hit
AGCGCTTC29015770.7099563972330115No Hit
CAGAGGAT28375750.6942964201461698No Hit
ATAGGAGG27887240.6823435820994008No Hit
ATATTACA27317500.6684032125086735No Hit
GGTGAGAT27247920.6667007325772613No Hit
ACGAGTTC27234940.6663831385917809No Hit
GGCACGTT27024640.6612375287962076No Hit
CTCCTTCA26840750.6567381175489038No Hit
CTCACGCC26689790.6530444358810972No Hit
AACGCTGG26656430.652228185083283No Hit
GGCGCCAT26537390.6493155203659028No Hit
CCAATCTA26306320.6436617112576615No Hit
CGCTACAA26164700.6401965602388832No Hit
ATATGGTT26087650.6383113047241474No Hit
CCAGATTC25825230.6318904253967372No Hit
TAAGCAAC25720960.6293391522945765No Hit
ACGTGCGC25647150.6275331729364629No Hit
CTTAGATT25445210.6225921151993347No Hit
CTGCTCAG24715870.6047466608564748No Hit
TATCTACT24573650.6012668290679515No Hit
CTCTTAAT24312390.5948743325620481No Hit
ATTGAGTA24093060.5895077771818146No Hit
GATGTCCA23872470.5841103921851171No Hit
AACTAGTC23797280.5822706470565904No Hit
GCCACGAC23659240.5788930912972896No Hit
CTGTTCGC23655460.578800602447897No Hit
TGCCTGAC23146920.5663576713711455No Hit
TCTACCGT23141430.5662233423279799No Hit
AAGCGCAC23082300.564776552469624No Hit
TCGTCTGG22701840.5554674763744085No Hit
GCAACAGC22647630.5541410688279604No Hit
ACATTCCT22373670.5474378293624576TruSeq Adapter, Index 27 (100% over 8bp)
GAGAGCGG22284210.545248926593499No Hit
TAATTGAT22255450.5445452283637287No Hit
TATATAGC22155520.5421001461627223No Hit
GGTCAACT21294410.5210305501043955No Hit
TTGGAACG21121100.5167900097635928No Hit
CATCCTCA20988670.5135497192014065No Hit
GGTGCGGA20715900.5068755965958974No Hit
CTGGCCTC20671010.5057772303394863No Hit
GTCTGGCG20293980.4965520793113121No Hit
AGACAGAG20016640.48976613817624653No Hit
CCGGTCTT19948080.4880886155534006No Hit
GCGCTGGC19901400.4869464516672505No Hit
GAGTGGCA19774240.4838351071993232No Hit
ATGCTAGG19422120.47521945279505656No Hit
TAACATTA19121220.46785704676800943No Hit
TAGCTGGT18904310.46254970382574695No Hit
GTCCTCAA18629160.45581734750025005No Hit
TCGAGCAA18388300.4499239971656719No Hit
GGAGAACC18128530.4435679579046349No Hit
CGGCCAAG18108100.44306807769482237No Hit
GAGCCGTC18027320.4410915567281728No Hit
GCTCACGT17715120.43345266287093076No Hit
GCCGTCCT17592250.43044628590667927No Hit
TAGAGTAG17529110.4289013795705286No Hit
ACCGTTCA17184920.42047975600638976No Hit
ATGTTGTG17086330.41806746085781366No Hit
GTTCGGAC17053510.41726442275276976No Hit
CGTTCGCG16523690.4043008137090672No Hit
AGAGTATT16427110.4019376991391363No Hit
ACCACCAA16330610.3995765420051714No Hit
AACTACCT16240250.3973656180815955No Hit
ACCAGCGG16185350.39602232764994083No Hit
AAGACACT16049420.3926963992642429No Hit
ATGCAACT15964160.3906102618834983No Hit
TGGCCTAT15897080.38896895182602303No Hit
TCGCGGAT15598220.3816564604161084No Hit
AGCATAGT15444860.3779040556693223No Hit
TGCATCAA15415070.37717515545149No Hit
GCCTCATT15368310.3760310341293739No Hit
GGCCTGCT15255500.3732708047378446No Hit
GTTGGTCG15238600.372857296390031No Hit
CTAACATA15197660.37185557853444007No Hit
GTCAACGT15130380.3702093748870498No Hit
CTGTAGCA15116240.36986339808006263No Hit
GGATCTCT15091390.36925536953312965No Hit
CCTTAGCC15023070.36758371921818167No Hit
GGTTGCAG15011920.367310901580423No Hit
TGCTGCCG14978700.3664980762955493No Hit
GCTCCAGA14915560.3649531699593986No Hit
CTATGGAG14368210.3515606377596503No Hit
GATATATG14279620.3493930221068218No Hit
ACTCGGAA14163910.3465618356615256No Hit
ATCATGGA14025790.34318232246625896No Hit
AGGAATAC13938620.34104945130182657No Hit
TATTAGAG13793410.33749646034407477No Hit
TGATAAGG13752290.33649033825756036No Hit
ACCTGAAC13500410.3303273511186683No Hit
GAAGCGAT13499090.3302950534252296No Hit
GGCCAATA13078380.32000114235962973No Hit
GTCAGATG12713840.3110815960216445No Hit
CGACAGTT12653520.3096056893032946No Hit
AGGCCGAC12497340.3057842849387077No Hit
TTGAGAGT12417560.3038322319216328No Hit
GAGGTGCG12382330.3029702260581138No Hit
CCAACAAT12321560.3014833087624552RNA PCR Primer, Index 36 (100% over 8bp)
AGGTGTAA12285480.3006005051417977No Hit
GATTATCT12175370.2979063392141202No Hit
CGCGTGAA12151280.29731690630886404No Hit
TGTACCTA12138240.2969978442464091No Hit
ATAACGCG12073860.29542259765278567No Hit
AACGAGAT12024710.2942199979311859No Hit
CTACGGCC11977310.293060217121342No Hit
ATCTGTCT11664990.28541838711015105No Hit
GTTGTGTG11490640.2811524001017906No Hit
CCGTACAC11477800.2808382316292506No Hit
CGAAGCAA11383580.27853286142031614No Hit
AGGCGTGC11373420.27828426705263654No Hit
TTCGGAAG11360980.27797988575992644No Hit
AGCACAAC11080210.2711100195578193No Hit
AGTTCCGG11037970.2700764933677812No Hit
CTCTATAG10877820.2661579512433824No Hit
CAATTAAG10769140.2634987744835968No Hit
TGGCTTCC10661050.26085403381406036No Hit
AGGATTGG10520350.25741139330889073No Hit
TTGTACTC10455560.25582611485594164No Hit
CTCAGAAC10325150.2526352495518964No Hit
CAACGTAA10253340.2508782060929325No Hit
AGATGCGA10037140.24558823539486802No Hit
TAATGATT9772890.2391225797197361No Hit
AGCCATTG9690610.2371093568287243No Hit
CAAGCACT9370850.2292854852726971No Hit
GTCATTAG9293800.2274002297579614No Hit
TGCCAGGT9162180.22417975823493067No Hit
AAGTTACG8992960.22003929180788875No Hit
CAGATTCA8919710.21824701450153713No Hit
ACTTCTAT8591550.21021761216908189No Hit
AGTTCGTC8424920.2061405177314386No Hit
TCTGCGCA8420970.20604386933061827No Hit
GGATACTA8352420.20436659138726804No Hit
CGAACGAG8126360.19883536670639643No Hit
AACTCTAC8118570.19864476137920897No Hit
TGTACGCC7838040.19178076748499984No Hit
GGATCAGA7837620.19177049094617843No Hit
ACATCCAG7716540.18880791161166577No Hit
GAGTAATT7699800.18839831813578417No Hit
CTCTCTGC7630990.18671467852554452No Hit
CCAGCCAA7526420.18415606503851123No Hit
CGCATATA7411850.18135277205573028No Hit
ACGCGACC7388600.18078389222811697No Hit
ACTAAGTG7366180.1802353207986507No Hit
TACGTCAG7171920.17548217691425663No Hit
AACCTTCT7098310.17368109114605812No Hit
TGTAGACG6996740.17119588150774911No Hit
CCTTACTG6995030.17115404131397624No Hit
CATCGAGC6793990.16623500473146527No Hit
CGAACTGC6731870.1647150557038808No Hit
CTGATCCT6675720.16334118033525769No Hit
ATTATCGT6333470.15496702459329398No Hit
ATCCTAAC6130190.14999317980373553No Hit
CGTGCCAC6096300.1491639609926467No Hit
TCGGCCAG5631580.1377932154662614No Hit
TATAACGG4650520.11378868885644135No Hit
TTCACTTG4647420.11371283821275957No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)