FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408347733
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATCG70761601.7328760338679288No Hit
AACAGTGA65541611.6050440520016307No Hit
TCCGCGAT59004891.444966758270212No Hit
GGCGTGTC58029871.4210895594711188No Hit
ACTTGTCC53631291.3133730315089076No Hit
GACGATGT52970321.2971865819076311No Hit
TTGCGTCG52300511.2807836501445693No Hit
ATGGCGCT52169661.2775792733493636No Hit
AATGCGAA47970131.174737267367173No Hit
CCTGGCCA47953041.1743187515136762ABI Solid3 GAPDH Forward Primer (100% over 8bp)
TCGTCGAC46184881.1310184009274271No Hit
CCATTGAC45513031.114565511742415No Hit
CAACAGCA42710991.0459465438002076No Hit
TGCTAACT41072281.0058162855039041No Hit
TACATGTC39785890.9743139678456352No Hit
TTGTGTAC38194700.9353474236135896No Hit
CTAGTCCG37939250.9290917258502327No Hit
GTCCATCC37518590.9187902115768571No Hit
ACCATAAG37060870.9075811374713815No Hit
ACTGAGGC36851600.9024563386029623No Hit
TAACTATG36754870.9000875241788105No Hit
CTCGTATC35162750.8610982052396995No Hit
GCGACAAG34875180.8540559229699459No Hit
AGTCTACC34865200.8538115234253056No Hit
GCAGTTAA34825720.8528447003769701No Hit
TGCTTCTT33982670.8321993059772906No Hit
AGACCATC32082250.7856600492012527No Hit
ACGGACGG31792180.7785565446006774No Hit
TACAGCCT31771740.7780559908238796No Hit
CAGGAATC31732730.7771006775737384No Hit
TAGCCACC31476350.7708222051033157No Hit
AGAACACA31186750.7637302103009349No Hit
CGCCGGTA30592420.7491757031500405No Hit
CAGATAGT30578680.7488392252198447No Hit
GATCGATT30457990.7458836559770983RNA PCR Primer, Index 42 (100% over 8bp)
TTACCTAC30298860.7419867321756382No Hit
CTCTCGAA29996820.7345900950550888No Hit
GTGAGTCA29681040.7268569800043435No Hit
TAGGTCTC28807120.7054556122636781No Hit
TAAGACTG28723780.7034147046434075No Hit
CACAGGCA26864340.6578790043141981No Hit
CTCTGAGA26610470.6516619990639204No Hit
AAGGCCTG26372620.6458373065095478No Hit
ACATACTC26245120.6427149676376434No Hit
TTAGGATC25984780.6363395189951991No Hit
GGCAACAC25600190.6269213205109185No Hit
AATATCAA25507020.624639686685857No Hit
CGGACATC25503730.6245591180984957No Hit
AGAAGTAG24933020.6105830395292043No Hit
ATCTCAGT24258040.5940534999860033No Hit
GCGGAGTA23562770.5770270800058538No Hit
CTGTAAGG23004890.5633651944383392No Hit
TCCGGTCT22978060.5627081563839611No Hit
CATACGTT22755230.5572512875931651No Hit
GCGCCTAA22277100.5455423943788614No Hit
TGCTCGAG22240630.544649283016835No Hit
CCACATAT22147370.542365445187864No Hit
TCAAGGTT22087450.5408980683627305No Hit
ACGCTATT22057540.5401656043967801No Hit
TGATTCGC21369610.5233189331799228No Hit
CGAATCGT21340330.5226018972413397No Hit
ACCGAACT21171450.518466206349675No Hit
GAAGGTGA20745890.5080446963078892No Hit
GTAATCTT20597880.5044200894339237No Hit
TTAGCAGT20222330.49522327089789425No Hit
ATATTCGG20215160.4950476852530978No Hit
TTGACCGA20049110.49098129803992324No Hit
CCGCGTAC19725150.48304786352272955No Hit
CAGAGTGC19283720.4722377141248878No Hit
TGTATATT19129050.46845001096161343No Hit
CCGAATAG18704090.45804319427922474No Hit
GCACTCTC18347060.4492999107699222No Hit
GACTAAGA18144620.4443423713093076No Hit
CAATCGTA18062620.4423342788583572No Hit
AAGCTCCA17960480.43983297931030757No Hit
GGTCGTAT17807230.43608005043093995No Hit
GTTAATGG17727950.43413856787592353No Hit
TGCTCAGC17672120.4327713507840143No Hit
GAGACTGA17507680.4287443907518889TruSeq Adapter, Index 15 (100% over 8bp)
TCCTCCTA17221140.4217273320824338No Hit
GCCTGGTC16328850.399876102654891No Hit
CAGCTAAC16281250.3987104294760466No Hit
GTATCTAG16251310.3979772308421264No Hit
ACACTGTG16206820.3968877182428242No Hit
CTAGCGGC16111090.39454339275100125No Hit
CTTGCTTG15862220.38844883216236686No Hit
ATGCGGCA15744250.3855598728155545No Hit
TCAATAAC15692760.3842989376899516No Hit
CTACAAGC15689340.38421518554138756No Hit
TCCGGCGA15592490.38184343244535657No Hit
TGAGCTCC15562280.38110362179970764No Hit
CGTATGAC15532390.3803716476124039No Hit
GCTGCATG14992820.36715815439582716No Hit
AGGTAGCC14988930.36706289244906865No Hit
TTGTGGCT14953270.36618961712222853No Hit
GTAGCACA14782290.3620024994726737No Hit
AGCTCACG14695010.359865105459028No Hit
GCATAACG14671420.35928741154539484No Hit
CGACTCTG14634990.3583952797406616No Hit
GTAGAATG14501460.3551252726068152No Hit
TGGCGCGA14466040.354257874623734No Hit
CGTTCATA14312320.3504934359461719No Hit
GATCGGCG14285150.34982807165480206No Hit
CATTCCAG13820510.3384495341376121No Hit
TCCGTATG13766380.33712394823066155No Hit
GATCTTCC13577190.3324908871234018No Hit
GTTCTAAT13339290.3266649701224128No Hit
TATAGGTG13245370.32436496959810474RNA PCR Primer, Index 39 (100% over 8bp)
CTTCTAGA13143000.3218580375956195No Hit
CTGTCATT13070050.32007156998224356No Hit
AAGTGGAG12609010.3087811926214367No Hit
TCGTGTCA12589620.30830635222358393No Hit
TGTCGGCT12550980.3073600998784044No Hit
GTCTCCAC12456260.3050405082082334No Hit
GAAGGCAG12449840.3048832892626834No Hit
AGGCGGTT12402930.30373451344714575No Hit
TGGAAGAT12389440.30340415775003216No Hit
AGAAGAGC12278650.3006910289373395No Hit
GCCGGTTG12269180.3004591187481871No Hit
CTCCAATG12175350.2981613222277886No Hit
CATTGTGA12106160.29646693299996846No Hit
GTAATTCC11515210.281995198440345No Hit
CGTTGTCT11400770.2791926850246527No Hit
ATGTCAAC11387210.27886061510227605No Hit
GAGTCCTA11371100.2784660984024613No Hit
GTCATACA11353000.27802284872731253No Hit
TGTTGCGT11342610.2777684087204177No Hit
GCCTACAT11271490.2760267558532032No Hit
TTATCGTC11136870.27273005578311854No Hit
GTCCGACT11071420.27112725516220754No Hit
TATACTAT10770410.26375584164195665No Hit
GCTTCTCA10537980.25806387910080547No Hit
CGTACACT10489080.25686637030993387No Hit
GTTAAGCC10376520.25410989608701956No Hit
TAGCATGG10147420.24849948169052283No Hit
GCAGATCT10061440.24639392328890436No Hit
GTGGTCGT10007540.2450739698363894No Hit
CCGGCTGA9832020.24077567243406248No Hit
ATGAATTG9635320.2359586994450144No Hit
ATTGCCAG9549920.2338673446241466No Hit
TACGAGCG9502250.23269995722003922No Hit
CCGCTGTA9198590.22526364802911736No Hit
GACAGTCG8865870.2171156904647246No Hit
GCCATCGC8773830.21486172913319446No Hit
AATTCATT8725660.21368209726292273No Hit
GTCGAAGC8666690.21223798492350146No Hit
AACACGTA8481680.2077072875533755No Hit
CAATACAT7696950.18849008768710368No Hit
CGGAGCTG7581210.1856557386593842No Hit
AGGATCTT7559630.18512726749973166No Hit
AGCCACGC7347130.17992336937989073No Hit
GCAGTGGT7196310.17622994860608177Clontech Universal Primer Mix Long (100% over 8bp)
GCTGTAGC7010750.17168578232317502No Hit
CACATTAT6941080.16997963840783709No Hit
CAAGAGAG6897170.16890432938928548No Hit
AACGTCGA6856210.16790126272110342No Hit
TCGTTCTG6727730.1647549246955168No Hit
ACGCATTA6355510.1556396543041418No Hit
AGATGACT6243570.15289836321927125No Hit
TCATTGCG5580910.13667052732235935No Hit
CAGTACCG5507920.13488308015169023No Hit
TGAGTAAG5479740.1341929820386685No Hit
AAGCCTGT5453890.13355994313797256No Hit
CTCGAACA5245710.12846183720579146No Hit
GCGCAATC5185260.12698148124652378No Hit
AACTTGAA5024190.12303704891634601No Hit
ATACCGAA5018650.12290138023124521No Hit
AATTATTG4531590.11097380085124656No Hit
CACACATG4486590.10987179889645671ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
GATACGAG4179250.10234537043456537No Hit
AGAACGGT4167530.10205836014767346No Hit
GTGTTAAG4086080.10006373660950384No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)