FastQCFastQC Report
Tue 28 Mar 2023
EGAF00001836280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422527086
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGTC257022206.082975707739599No Hit
CGGTATAT176790644.184125606565256No Hit
ACAGTGCC172993974.094269355314182No Hit
TTATACGT152434643.60768918847489No Hit
ACAACCTT150305943.5573089863403453No Hit
TCTGTGTT130591933.0907351108847023No Hit
GAGAAGCT125859342.9787283270166496No Hit
GATGGTAC115113782.724411849894991No Hit
ACGACGAT90108902.1326183098235743No Hit
CAGCACAA90020942.1305365497917452No Hit
GCACGTCC75966651.7979119568206807No Hit
CTCTAGTT73774371.7460269990833204No Hit
ACTTAAGG72265301.7103116556177418No Hit
ATACGTTA68337421.6173500413178246No Hit
AAGGTGTT64667801.530500697888987No Hit
TGCGGATT63514631.5032084830651544No Hit
CGTATCCA52368351.2394081169035398No Hit
CTTCGCAA50630211.1982713458516598No Hit
GTGCCACG47516631.1245818688177545No Hit
ATTAGGCT43520701.0300097068806615No Hit
CCTAACCT37846670.8957217478834008No Hit
CGGCTTGA36521230.8643523979904095No Hit
TGTGTCGA34962890.8274709754346967No Hit
TGAATTGA34896420.8258978218499345No Hit
GCGGCTCC34403700.8142365576061554No Hit
CTTCTGCT32935900.7794979562564659Illumina Single End Adapter 1 (100% over 8bp)
AGCAGGAA30924010.7318823106171234Illumina Paired End Adapter 2 (100% over 8bp)
TTGCAGAA30612100.7245002986625099No Hit
ACTGTCAT29623030.7010918585228877No Hit
GTAAGAGA26553960.6284558050794405No Hit
GTGTAGAT24117360.5707884961486233Illumina Single End PCR Primer 1 (100% over 8bp)
CTCATAGG23598070.5584983964791312No Hit
CCTGGTGC23252200.5503126490688457No Hit
CTGCCTTA22818420.5400463249828202No Hit
CCAAGACG22297290.5277126778092518No Hit
CATGTATT21069770.4986608124810252No Hit
GGTTAATG21015960.4973872846580065No Hit
ACTGGTTA20810530.4925253478306003No Hit
CAACTCGA20526530.4858038852448858No Hit
TGGCCGCA20056670.4746836514050131No Hit
CGATCTAA19531930.4622645659218164No Hit
GCAAGCAC19384710.458780292253264No Hit
TTCCTTAT19005390.44980287961941445No Hit
CTGCGCTT18795730.4448408308668761No Hit
ATACAGTC18736530.4434397372574595No Hit
CCTATACC18106220.4285221137278759No Hit
GTGTGTGT18075300.4277903263224171No Hit
TGGTTCCA17507840.4143601813967495No Hit
CATCAGTG17440570.41276809411456283No Hit
CTAGGCAC17428280.4124772251878782No Hit
TCTCTCAG16620010.3933478006662039ABI Solid3 Adapter A (100% over 8bp)
TCAATCGG16606790.3930349213162633No Hit
GGCTACGG16586100.3925452485666209No Hit
CTGATTAC16196440.38332311789355916No Hit
CTCAATTC15969960.3779629881526696No Hit
ACCAGGCC15530890.36757146499242416No Hit
CTATCGCT15455390.36578459729798246No Hit
CATATCTC14846150.35136564002431786No Hit
AACCGTTC14544660.34423023947865916No Hit
AGTCTGAT14426570.34143538906757803No Hit
GCTAGTCT14163070.3351991024783675No Hit
GCTGACAA14095060.3335895015260631No Hit
CCATGCGG13963580.33047774835433863No Hit
AGCGTTAT13541320.32048406951122654No Hit
AAGCATCC13282450.3143573616958606No Hit
TCCAGATC13206830.3125676539468052No Hit
ACACGAAT13095220.30992616648486293No Hit
TGCCGTAG12889170.3050495560419528No Hit
TACCTCGC12778810.30243765248223636No Hit
TCAACTTA12741110.3015454019911045No Hit
TGTAGCAC12350910.29231049107228124No Hit
CTCGGCCT12346770.2922125091881092No Hit
CGCCAGCC11910720.28189246073564145No Hit
CGACTTAC11751310.2781196848525824No Hit
CTGTGTTG11593760.2743909298160355No Hit
ACGATTCT11202060.2651205182145412No Hit
AATCCAAG11079520.262220349111536No Hit
TATGAGGT11035150.261170238918127No Hit
TACCTGCA10627760.2515284901758937No Hit
AGCAACTA10460410.24756779734589604No Hit
GTGGTGTC10407000.24630373637158967No Hit
TTGCTCTA9967210.23589517288366219No Hit
TCGGAGCT9853130.23319522763092163No Hit
CACCGGAG9769420.23121405286666047No Hit
CCACCAGG9583890.22682309176268997No Hit
AAGATGGC9373170.221835955861064No Hit
TCTTAGTA9361340.22155597381039852No Hit
GTTCACTG9295480.21999725716992258No Hit
GTCACAAT9293720.2199556030355886No Hit
CGAGTTCT9104240.21547115680058437No Hit
AACGCGCC8951420.21185434725006008No Hit
ATTAGTGC8747840.2070361945979482No Hit
GCCAACTG8560620.20260523605816835No Hit
ATGAGACG8329950.19714594107701772No Hit
TTAAGTGG8077080.1911612359923359No Hit
GCGTCGCT7950250.1881595349368916No Hit
GTCCGCGG7920160.18744739124251078No Hit
AGAGATCG7546590.17860606455890024No Hit
ATCTGCTG7420090.1756121736536436No Hit
GCTACCTC7312840.1730738748426651No Hit
CGGTTGTT6987990.16538561033220955No Hit
GCCACTGT6949510.16447489948608882No Hit
TTAGGTCA6929540.16400226706412852No Hit
AGACTCAA6867030.1625228352816179No Hit
CCTGTGCG6716110.15895099326247691No Hit
ATTACGAC6235420.14757444449371937No Hit
ATAGCCTA6168830.14599845085434357No Hit
TAGGTGAA6083620.14398177540741142No Hit
TGGCACAG6007810.14218757090521766No Hit
AAGAACGT5916410.14002439597446303No Hit
CGGTCGGA5844060.13831207971363046No Hit
GCGCTCAT5748190.13604311274851622No Hit
CGTAGTTC5496260.1300806547583082No Hit
CCATAAGA5320260.12591524132490764No Hit
GTGCTGTC5261390.12452195786567871No Hit
AGTGGCAA5235850.1239174995754No Hit
CCAGGATA5209890.12330310109397342No Hit
NTGATGTC4944600.11702445035677547No Hit
ATGTAATA4591760.10867374310767855No Hit
GAGCGTTA4568940.1081336593886433No Hit
AGTGCTCA4568610.10812584923845568No Hit
GCATCGAA4491390.10629827409455118No Hit
GACATCTA4471590.10582966508329361No Hit
CGGTGTAT4258610.10078904148644331No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)