FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22446029
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGTC2644841.1783108718250341No Hit
AATGCGAA2202170.9810955871080804No Hit
GAGAAGCT2137910.9524669151946654No Hit
GGCGTGTC2078920.9261860973270595No Hit
GACGATGT2069640.9220517357435473No Hit
TCCGCGAT2056920.916384809090285No Hit
ACTTGTCC2010090.8955214305390053No Hit
CGGTATAT1876860.8361657200033021No Hit
TGCTAACT1862540.8297859723873653No Hit
ACAGTGCC1781930.79387316126162No Hit
AGTCTACC1703220.7588068250290508No Hit
TTATACGT1702860.7586464403124491No Hit
ACCATAAG1677570.747379413971175No Hit
GATGGTAC1673220.7454414319789037No Hit
ACAACCTT1667920.7430802125400444No Hit
AGAACACA1583830.7056170158204821No Hit
CTAGTCCG1582090.7048418230235736No Hit
TCTGTGTT1569980.6994466593623309No Hit
ATAGGAGG1436940.6401755963159452No Hit
CCATTGAC1435690.6396187049388558No Hit
TTAGAGAT1373390.611863238704717No Hit
AATATCAA1357220.6046592918506877No Hit
ATGGCGCT1291710.5754737285601832No Hit
GTGAGTCA1284110.5720878289874792No Hit
GCACGTCC1246950.5555325621293637No Hit
CAGCACAA1160690.5171026019791741No Hit
ATACGTTA1146490.5107763159354378No Hit
CCGAAGGT1069740.47658318538214495No Hit
TTCGCATA1038580.46270099713405877No Hit
ACAGCGGA1027360.45770234013330374No Hit
ATATTACA1008710.44939352078712896No Hit
GCAGTTAA1007370.448796533230889No Hit
ACGACGAT986990.4397169762188225No Hit
CGTATCCA981840.43742258374521387No Hit
TAATAGGC971490.43281152314291316No Hit
ATCATCTC969800.4320586060010882Illumina PCR Primer Index 7 (100% over 8bp)
CTGTTCGC965990.4303612010837195No Hit
TTGCGTCG952600.4243957806523372No Hit
GTGCCACG940330.4189293348948271No Hit
TAGCCACC908340.4046773707723535No Hit
AATTGCTA894080.398324353942517No Hit
ACGGACGG886000.3947246080810107No Hit
ACTTAAGG882290.39307175447380915No Hit
CAGATAGT880240.3921584526153824No Hit
CGCGCGCT838300.37347363313127685No Hit
ACCTCGCA833900.37151337548392194No Hit
CTGCTCAG831650.37051097100516084No Hit
CGTCGTGG828510.3691120598659121No Hit
CTTCGCAA826420.36818093748341857No Hit
AAGACACT821540.3660068335472613No Hit
GTTCGGAC815100.36313772917249637No Hit
GGCCGCTC814480.36286151104946No Hit
CGACAGTT811460.3615160614824119No Hit
CCTTACTG809780.36076759947160364No Hit
TTGAGAGT805860.35902118811305106No Hit
CCGACCGC789430.3517014078525872No Hit
CAGAGGAT771310.34362871045029836No Hit
GTTGCAAC770790.3433970436374291No Hit
CAAGACGC770620.34332130641014497No Hit
TTAATACT755740.33669207145727204No Hit
TGATAAGG752780.3353733526763242No Hit
GATGGCGT742770.3309137665285918No Hit
CTCTAGTT736640.32818277121534506No Hit
TAGGATCA731680.32597302623105406No Hit
TAACCTCT724380.32272078058885156No Hit
ATCGCTAA670910.29889919504247275No Hit
TTCTTGCC669390.29822201512793195No Hit
AACCTTCT666900.29711268750476977No Hit
AAGGTGTT664580.29607909710889174No Hit
TGCGGATT659290.29372233280104915No Hit
GGTGTACG652020.2904834525518968No Hit
AACGCTGG645230.28745841859154686No Hit
CATTCCAG638310.28437546792797963No Hit
ATATACAA636620.28362255078615467No Hit
ATTAGGCT636020.28335524292515174No Hit
CTCTATAG633530.2822459153019895No Hit
CCTAACCT630510.2809004657349414No Hit
CAGGAATC626040.2789090221704694No Hit
TGAATTGA612690.272961422263154No Hit
CCAGATTC610030.2717763574127076No Hit
GAGTGAAC606250.27009231788838906No Hit
CTTCTGCT587830.26188596655559876Illumina Single End Adapter 1 (100% over 8bp)
TTGCAGAA580650.25868718248559686No Hit
GAGAGCGG580140.2584599708037444No Hit
TGGCCTAT577120.25711452123669626No Hit
CTCTCTGC576450.2568160274585763No Hit
ACTACATA572840.25520772516154194No Hit
ACGCGACC570340.25409394240736305No Hit
TCACCGAG568300.253185095679953No Hit
CGCCTCAT566090.25220051172525887No Hit
CCGTACAC565990.2521559604150917No Hit
GGCGGAAC563750.25115801106734736No Hit
GTTGTGTG560970.24991948464470043No Hit
CACAGGCA555400.24743797666838974No Hit
GTAGCACA554500.24703701487688534No Hit
AGGAATAC553140.246431117058612No Hit
TTGTACTC549880.2449787443471627No Hit
CTCCTTCA539670.24043005557909594No Hit
ACAGCACG528390.23540466779224067No Hit
TGCAATCG523470.23321274333201655No Hit
GTAGCTTC522520.23278950588542854No Hit
CTTCTAGA520070.2316979987863332No Hit
GTACTATA513630.2288288944115683No Hit
TAACATTA512680.2284056569649803No Hit
TGTGTCGA511900.2280581567456765No Hit
CTGTCATT511320.22779975914670697No Hit
AAGTTACG508670.22661914942727732No Hit
GCGGCTCC506740.2257593091410512No Hit
CCAATCTA505970.22541626405276405No Hit
AGCAGGAA500850.22313523697220566Illumina Paired End Adapter 2 (100% over 8bp)
GTGCGGTG500320.2228991150283197No Hit
ACTGTCAT500120.22281001240798537No Hit
AGGATTGG498830.22223530050682908No Hit
GTAAGAGA498820.22223084537581236No Hit
GGTAATTA497690.2217274155709235No Hit
CGGATGCG491600.2190142407817436No Hit
GCATGTAT490320.218443984011604No Hit
GGCAACAC488580.21766879121469548No Hit
GGCTCTTA481030.2143051672970751No Hit
GATATATG477290.21263894829682348No Hit
TATAGGTG477250.2126211277727566RNA PCR Primer, Index 39 (100% over 8bp)
CAACTCGA470870.209778754184092No Hit
CTCATAGG465660.2074576309243831No Hit
TAGCTGGT456550.20339900656815513No Hit
GTGTAGAT455840.20308269226596828Illumina Single End PCR Primer 1 (100% over 8bp)
CTGCCTTA454630.2025436214129457No Hit
CTGTAAGG453750.2021515698834747No Hit
ATATTCGG449050.2000576583056183No Hit
ACGCTATT447180.1992245488054925No Hit
TGGAAGAT444520.19803948395504611No Hit
ATCTCAGT443230.1974647720538898No Hit
ACTAAGTG440790.19637772008581117No Hit
TCCGTATG435520.194029866040002No Hit
TCGTGTCA434070.19338387204257823No Hit
AACGAGAT431200.19210524944078083No Hit
GGTTAATG430110.1916196401599588No Hit
TAATGATT428130.1907375242186491No Hit
GCGGAGTA422620.18828274702843875No Hit
CGAACGAG421510.18778822748558333No Hit
AGACAGAG420390.18728925281171116No Hit
GGCACTCC420100.1871600540122264No Hit
GGTTGGCC414910.18484784101455096No Hit
GGCGTTGA414560.1846919114289659No Hit
GCTTCTCA410570.18291431415329634No Hit
AGGTTATC410290.1827895704848283No Hit
CGATCTAA409840.18258908958907608No Hit
ATTGAGTA408560.18201883281893647No Hit
GCGCCTAA406050.18090059493374083No Hit
CCAAGACG405800.18078921665832295No Hit
ATACAGTC401030.17866411916334957No Hit
CCGACACA400160.1782765227648953No Hit
GTGGTACC399270.1778800161044076No Hit
TCGGCCAG399080.17779536861509002No Hit
TTCCTTAT398580.17757261206425423No Hit
CCGAATAG397610.1771404643556328No Hit
TTAACCAG396470.1766325794197272No Hit
CAGATTCA396240.17653011140634275No Hit
CGGCTTGA387350.1725694999324825No Hit
AATCTGTA382880.17057805636801057No Hit
CATCAGTG381900.17014145352837243No Hit
TCAAGGTT378280.16852869610032134No Hit
AACAGTGA377190.16804308681949934No Hit
TCCAACCA376910.1679183431510313No Hit
GGAGAACC376020.16752183649054359No Hit
GCCGGTTG375110.16711641956802248No Hit
TTCACTTG373140.1662387587577295No Hit
CATGTATT369520.16462600132967842No Hit
GGTGCGGA369480.16460818080561154No Hit
ATAACGCG368230.16405128942852207No Hit
TGGTTCCA368200.16403792403547193No Hit
GCATCCGT365690.1629196861502763No Hit
AGCTGTGG363200.1618103585271141No Hit
CCTGGTGC363170.16179699313406393No Hit
GAGTATAA362280.16140048647357624No Hit
TTAGCAGT361990.1612712876740915No Hit
TCCGGTCT360550.1606297488076844No Hit
TTGTGGCT355290.15828634989289198No Hit
GTTAATGG354830.15808141386612307No Hit
ACTGGTTA354080.15774727903986938No Hit
ACCACCAA353320.15740868908259897No Hit
CTCAATTC352350.15697654137397757No Hit
CATATCTC349480.15569791877218014No Hit
AGCAAGCT348430.15523013001542502No Hit
GACAGTCG348280.15516330305017426No Hit
AGTGTCTC348080.15507420042983994No Hit
TGTATATT346670.15444602695648305No Hit
TGTAGACG345870.1540896164751458No Hit
TCTCTCAG344080.15329214802315366ABI Solid3 Adapter A (100% over 8bp)
CCGCGTAC342690.1526728848118302No Hit
CAACCGGT336910.15009781908416853No Hit
AGGTAGCC334640.14908650434337406No Hit
CTGCGCTT334050.14882365161338781No Hit
ATTAATCA331920.1478747087068274No Hit
GCAAGCAC331680.1477677855624262No Hit
GTCATACA329700.14688566962111652No Hit
CCTATACC328000.14612829734827484No Hit
CTCACCTT326780.14558477136423553No Hit
CGAGCCTT325440.14498778380799562No Hit
CCGGTCTT323160.14397201393618445No Hit
AACTAGTC322690.1437626227783988No Hit
ATGTTGTG321510.14323691731842636No Hit
CTACAAGC320940.14298297485047354No Hit
TTACAGCG319370.1422835192808492No Hit
AACTACCT317530.1414637751737735No Hit
CAACAGCA316480.14099598641701835No Hit
GGTTGCAG314550.14013614613079223No Hit
GGAAGAAT313190.1395302483125189No Hit
AAGCATCC312600.13926739558253265No Hit
CCTGGCCA311740.1388842543150951ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GTTCAGGA311050.13857685027494174No Hit
ATCTGTCT311030.13856794001290829No Hit
GTATGCCT310570.13836300398613938No Hit
GGATCTCT310510.13833627320003908No Hit
CCTACTCG310400.1382872667588552No Hit
TATCTACT309750.13799768324276868No Hit
TGGCCGCA309670.13796204219463495No Hit
CTAGGCAC305880.1362735475392997No Hit
AGGCGGTT303310.13512857886800378No Hit
TCATTCAA302570.1347988991727668No Hit
CGACTCTG302570.1347988991727668No Hit
CACTCTCG301510.13432665528499496No Hit
TCAATCGG300710.13397024480365768No Hit
ATGCGGCA298090.13280300047727817No Hit
ACACGAAT297210.1324109489478072No Hit
TTCGTTCG296080.13190751914291834No Hit
AGTCTGAT295570.13168030746106582No Hit
GGCTACGG295300.13156001892361452No Hit
GCGCTGGC293620.13081155691280627No Hit
CATAATAA293550.13078037099568926No Hit
TCAATAAC292380.13025912066673354No Hit
CTATCGCT291870.13003190898488104No Hit
GGTCGTAT290930.12961312666930977No Hit
GTGTGTGT290430.12939037011847399No Hit
GCGAGATA289720.12907405581628714No Hit
GCTCACGT289140.12881565821731764No Hit
CGTATGAC288860.1286909145488496No Hit
AGCATAGT285480.1271850802651997No Hit
TCAACTTA282120.1256881562435832No Hit
GTCCATCC279970.12473030307498935No Hit
GCCTCATT277330.1235541484865764No Hit
TGTGAACA276900.12336257785285762No Hit
AACGTCGA275850.12289478909610248No Hit
CTGATTAC275470.12272549411746728No Hit
GGAAGGCG275140.12257847479391568No Hit
GATTATCT271600.12100135841399831No Hit
GATTACGC269930.12025735153420677No Hit
ATGTCAAC268080.11943315229611438No Hit
TAACTATG267600.11921930600731204No Hit
AACCGTTC266200.11859558766497183No Hit
TACATGTC265170.11813670917025011No Hit
CGTTCGCG264800.11797186932263164No Hit
GTCAACGT263160.11724122783589026No Hit
TCGGTAAT261810.11663978514863363No Hit
GTAGAATG260290.11596260523409284No Hit
GACCACTT259060.11541462411903683ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TAATTGAT259020.11539680359496995No Hit
CGAGAGGA258600.1152096880922679No Hit
TAATACCA258160.1150136623275324No Hit
TATTAGAG257920.11490673918313124No Hit
CGCTACAA257070.1145280530467104No Hit
AATCCAAG257050.11451914278467698No Hit
AGACCATC256710.11436766833010864No Hit
GTTATCCG256360.11421173874452359No Hit
AGAGTATT255930.11402016811080481No Hit
TAACGGTC255660.11389987957335349No Hit
TCCAGATC255060.11363257171235054No Hit
GATCGGCG254410.11334298819626404No Hit
TTCCGGTT254300.11329398175508015No Hit
CTCCAATG254120.11321378939677927No Hit
TACGAGCG253770.11305785981119423No Hit
TGCCTGAC252950.11269253906782353No Hit
CGTCCGAA251470.11203317967734962No Hit
TGATCCAT249910.11133817923874197No Hit
ACCAGGCC249450.11113324321197304No Hit
GCTGACAA249240.111039685460622No Hit
GACGCTCA245420.1093378254122366No Hit
TGCCTAGA245280.10927545357800258No Hit
TCGCAACA245170.10922644713681873No Hit
GGTCCGTG245060.10917744069563484No Hit
CAATATGG244510.10893240848971548No Hit
CTACGGCC244040.10872301733192984No Hit
CCTTAGCC243540.10850026078109408No Hit
CAGCGTCT242560.10806365794145593No Hit
TCGTCTGG242190.10789881809383745No Hit
GCGTACGA241980.10780526034248643No Hit
CCGGTAGG241910.1077740744253694No Hit
GTCACAAT239970.10690977900812655No Hit
GCGACAAG239350.10663356088509018No Hit
GTAATTCC239220.10657564418187289No Hit
GGTCAACT238990.10647317616848842No Hit
GTCTCCAC237810.10594747070851597No Hit
AAGCAGGA237440.1057826308608975No Hit
GCCACGAC237440.1057826308608975No Hit
TACCTCGC237040.10560442562022887No Hit
CTCGGCCT236510.10536830367634292No Hit
CCATGCGG236310.1052792010560086No Hit
CCACTACA235570.10494952136077165No Hit
CAACGTAA235090.10473567507196929No Hit
CGCCAGCC233930.10421887987403028No Hit
GCTAGTCT233260.10392038609591034No Hit
ACTCGGAA232650.10364862310389067No Hit
GACCTGTG231710.10322984078831939No Hit
TCTCTATA230750.10280214821071468No Hit
TGTAGCAC230740.10279769307969798No Hit
CATCCTCA230720.10278878281766454No Hit
TACTCCGG230530.10270413532834693No Hit
CTCGTATC230430.10265958401817978No Hit
GGCGCCAT228500.10179974373195366No Hit
TGCCGTAG228210.1016705449324689No Hit
AGATGGAC227770.1014745191677334RNA PCR Primer, Index 16 (100% over 8bp)
CCAACAAT227410.10131413445113163RNA PCR Primer, Index 36 (100% over 8bp)
CATTGTGA227360.10129185879604807No Hit
TAGTCGTG227340.10128294853401464No Hit
GGATCAGA226930.1011002881623293No Hit
TCAGGTAC225950.10066368532269117No Hit
GCTCCAGA225390.10041419798575509No Hit
CCGTGGCG224490.10001323619425068No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)