FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406997142
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGTT47350031.163399570014671No Hit
GCGAGATA46701881.147474396761243No Hit
TTACAGCG45636961.1213091024604787No Hit
TTAGAGAT43774741.0755539900081166No Hit
CAGGCGCA38916780.9561929552812437No Hit
TAATACCA37445110.9200337333081322No Hit
CGTGTGGT36162330.8885155758661322No Hit
CTGACTGG35845460.8807300175095579No Hit
TTCGCATA33436740.8215472923394631No Hit
GGCCGCTC33145110.814381885757812No Hit
TTAATACT33072910.8126079175268508No Hit
ACAGCGGA32777770.805356269553362No Hit
CCGAAGGT32001910.7862932364276897No Hit
TTCTTGCC31862830.7828760134143644No Hit
ATGTGCAT31631190.7771845729570259No Hit
GTTGCAAC31415430.7718833072297102No Hit
GATGGCGT31211540.7668736897420277No Hit
CAAGACGC30241350.743035930212994No Hit
CTATCCTG30236660.7429206959885728No Hit
GCACCACT30019050.7375739753966134No Hit
ATCATCTC28470340.6995218654385539Illumina PCR Primer Index 7 (100% over 8bp)
TAATAGGC28234980.6937390238479857No Hit
CGTCGTGG28002770.6880335783782973No Hit
CTGAACTA26815480.6588616290578276No Hit
CGCCTCAT26538540.6520571586716449No Hit
ACCTCGCA26182060.6432983748077523No Hit
GGCGGAAC26068440.6405067090127134No Hit
TCACGCCG24953390.6131097107310891No Hit
GGACACAT24842050.610374064985449No Hit
GACGCCTC24456140.6008921802207643No Hit
GTAGCTTC24298570.5970206542629727No Hit
AGTGTCTC24272730.5963857603697865No Hit
TCTGACCG24206900.5947683042943825No Hit
GCATGTAT24100650.5921577208520054No Hit
AATCTGTA24091340.5919289723169604No Hit
GGTGTACG24074700.5915201242371377No Hit
TAGGATCA23770970.5840574182705194No Hit
AATTAACA23691150.5820962251376203No Hit
CCATCTCC23269940.5717470124151388No Hit
TAACCTCT23012100.5654118327936563No Hit
ATAACTGA22751430.5590071195143675No Hit
TGACGGAA22534100.5536672785775975No Hit
TCCTTACA22408840.5505896156882595No Hit
ATCGCTAA22404840.5504913348998407No Hit
TGCAGTGT22291430.5477048288461938No Hit
AACGGATA22249440.5466731262697663No Hit
TCGGCAGC22119040.5434691725673101No Hit
GTGGTACC22003850.5406389315628167No Hit
GGAAGAAT21796030.5355327532005126No Hit
GCGTACGA21781900.5351855763154229No Hit
TATCAAGA21644550.5318108597430888No Hit
ATTAATCA21580610.5302398413402126No Hit
CTTGCCGA21483190.5278462127382703No Hit
TGTGAGTC21399320.525785510307097No Hit
GGCTCTTA21191350.5206756464152271No Hit
GCAGCTGG20953380.5148286766102156No Hit
TTCACGGT20938480.5144625806733553No Hit
CTTGCGAT20623710.5067286197307007No Hit
GTGCGGTG20606570.5063074865523257No Hit
TGTGAACA20449330.5024440687595787No Hit
TTATGCTA20338510.499721199516433No Hit
CGCGTCGG20281060.4983096416927664No Hit
TCACCGAG20116830.4942744782222574No Hit
AATCGCGG19915560.48932923465098926No Hit
ATATACAA19672520.4833576939466568No Hit
GGTAATTA19518050.47956233559988976No Hit
AGAGCCGC19429550.4773878731561216No Hit
GAGTGAAC19317620.47463773099418965No Hit
CTTACAAG19236720.47265000204841734No Hit
CGCGATTA19226000.47238660953545464No Hit
GGATTGTG19062280.468363976865469No Hit
CTGGATGC19051810.4681067269017825No Hit
GTACTATA18994880.46670794558061046No Hit
GATTACGC18986820.4665099097919464No Hit
TAGATCCG18749780.4606857902702422No Hit
CCAGCGTG18730420.46021011125429473No Hit
ATCGGCGC18635330.4578737312116064No Hit
GACCACTT18622030.45754694759011355ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GGCGTTGA18596500.4569196704580299No Hit
TCGATTGC18572620.456332934151169No Hit
GGTCCGTG18567640.4562105745695875No Hit
CCGGTAGG18508490.454757247410843No Hit
GTTATCCG18347930.4508122565637082No Hit
TCTAGTGA18314550.44999210338435247No Hit
TGTGGAGC18107510.44490508977579013No Hit
ACTACATA18065570.4438746157092179No Hit
GTTCAGGA18042670.44331195819551966No Hit
CAGTGGTC17568950.4316725644230691No Hit
ACAGGCTG17550810.4312268610475894No Hit
TGTCCTGC17526260.43062366270866836No Hit
GGCACTCC17442740.42857155984648165No Hit
GTTGCCTT17432430.428318241114332No Hit
TACTCCGG17230370.42335358708735105No Hit
CCGACACA17215170.4229801200913592No Hit
GCGCGAGG16942380.41627761602316116No Hit
CTAATGAT16906900.4154058654298855No Hit
ATCCTCCG16896440.4151488611681701No Hit
CGCACCGA16893450.41507539627882695No Hit
AGCTTCAC16757650.4117387635120052No Hit
TGATCCAT16607400.40804709139702017No Hit
CGGATGCG16433400.40377187710079787No Hit
GGTAGCGA16373070.4022895571094698No Hit
GCATCCGT16352050.40177309156632846No Hit
CACTGCTT16192750.39785905916754566Clontech Universal Primer Mix Long (100% over 8bp)
AGCAAGCT16141910.3966099103467414No Hit
TTAAGGAC16139130.3965416051987903No Hit
ACTCTTGT16106190.39573226290616065No Hit
CCACTACA16060770.39461628455366404No Hit
TAATTAGA15917030.39108456442183076No Hit
TCCGACTT15756300.38713539664118823No Hit
TACTGTTG15745730.3868756896577913No Hit
CGAGAGGA15738600.38670050415243457No Hit
AAGCAGGA15711560.3860361260227228No Hit
CGTAATGT15659560.38475847577327704No Hit
ATTGACCT15649130.38450220861747475No Hit
TTAACCAG15561050.38233806565649053No Hit
TCGCAACA15371800.37768815585442117No Hit
TCTATGAA15322320.3764724225016794No Hit
CGAGCCTT15189610.37321171164391126No Hit
AGGCCAGA15074210.3703763108980259No Hit
AGTTACAT15005560.3686895668667865No Hit
CCTACTCG14977580.36800209275179624No Hit
CTTATGTA14909110.3663197713560357No Hit
CAGGCTAG14847850.36481460108140024No Hit
TCCAACCA14809850.36388093359142065No Hit
GACTTATC14688360.36089590034516755No Hit
GGTTGGCC14615050.3590946591954201No Hit
AAGTGAGA14607370.35890596008165576No Hit
GTCTTGTA14600500.35873716282754636No Hit
TCTCCACG14485700.3559165041999238No Hit
AGATGGAC14145990.3475697625414775RNA PCR Primer, Index 16 (100% over 8bp)
CGGAGAGA13931070.34228913577972986No Hit
TCGCACGC13785090.338702378406382No Hit
GCGGAAGT13781230.33860753744555777No Hit
ATCGCGTG13653470.335468449063458No Hit
CTTAACAC13577050.33359079460071495No Hit
TGGCTGTG13317480.32721310853824126No Hit
ACGTGGTA13230200.32506862173494083No Hit
GGCCTAAG13211370.32460596492345883No Hit
AGGTTATC13085700.321518228253308No Hit
ATGGTCAA13060870.32090815025919767No Hit
TACTCAAT12989040.3191432730011652No Hit
CGCTTACC12951710.3182260675432458No Hit
GGACCGGC12697100.3119702496584116No Hit
CGAGGAGT12668370.3112643478955928No Hit
TAACGGTC12662700.3111250348780091No Hit
ATAATATG12454640.3060129596683998No Hit
CGGCACTC12424270.30526676278232934No Hit
CATCTCCG12266200.30138295172598534No Hit
CGTGATCC12156620.2986905495272495No Hit
ATAGGTAT12131120.29806400950107903No Hit
TACACACA12116930.29771535840416297No Hit
AACCAACG12002480.2949032993455271No Hit
GAGGTATA11972010.2941546454397461No Hit
GATATTGT11845730.2910519209493614No Hit
GGCCGTCA11780400.28944674997250963No Hit
GAGTATAA11646360.28615336075259223No Hit
ACTCCGTT11602850.2850843114765656No Hit
GGAATATC11474430.2819290067643767RNA PCR Primer, Index 37 (100% over 8bp)
TGAGCGTA11349810.27886706880118584No Hit
GCCATGCG11260990.2766847438943441No Hit
TCGGATTG11090720.27250117643332245No Hit
ATTCTGGC10805870.2655023557880414No Hit
ACTCATAG10760340.26438367471386326No Hit
TCATTCAA10621170.26096424038279853No Hit
GGCTGAGT10528290.25868216047571163No Hit
ATAAGCCA10412520.25583766875689756No Hit
GACCTGTG10388940.25525830350916817No Hit
TGGAGTTA10350840.2543221789994781No Hit
GGAGTGCA10111700.248446462063854No Hit
GTGTCGGC9563150.2349684804420568No Hit
TAGGCATT9555730.2347861695795397No Hit
TACTGCAC9446630.23210556107541416No Hit
GATACACC9276280.2279200279986241No Hit
GAACAAGT9078440.2230590602034252No Hit
ACACTAGC8962990.22022243094768465No Hit
GCTGGAAT8845190.21732806172874794No Hit
CGCAAGTG8789620.21596269587563838No Hit
TTACAATT8759880.21523197821374385No Hit
GTTACCAA8440150.20737614909344992No Hit
TGTCTTAA8435430.2072601777631156No Hit
GACTCGGT8370860.20567368013606346No Hit
AGTGGATG8068380.19824168691582605No Hit
ATTCGATC8040710.1975618295619383No Hit
ATAGTTGC7973680.19591488925000855No Hit
AGCGAAGA7768730.19087922735339505No Hit
CGATGCTC7424780.18242830805922466No Hit
ACCTAGGT7083270.1740373400459898No Hit
GCCTTAGG6945940.17066311487759783No Hit
TCGTGAAG6714500.16497658845968016No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)