FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29827679
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGA4835871.6212692915194644No Hit
GTACTT4690651.5725829689933297No Hit
TATTCT4560601.5289825265988681No Hit
CCTGAC4531411.5191963142690386No Hit
GATTCA4529531.5185660272125094No Hit
TGTGAT4509831.5119614234818606No Hit
AATTGC4378911.4680693056942178No Hit
GTCTCA4369561.4649346333652042No Hit
GTTACA4270011.4315595926857063No Hit
CAAGGA4208461.4109243967658363No Hit
AGTTCC4143121.3890185689607295No Hit
ACCGCT4098791.3741565342714062No Hit
ATTGCT3993341.33880346506344No Hit
CCTCGG3977591.333523134669647No Hit
TAGAGC3936911.3198847955953932No Hit
CTGGAG3897331.3066152415010233No Hit
CGAATT3829381.2838343875163738No Hit
TATGGT3822131.2814037592398657No Hit
ATTCGA3768301.2633567633606357No Hit
GGATTC3712891.244780058146663No Hit
GAGATA3709881.2437709283380716No Hit
TTAGCG3661581.2275779151304396No Hit
TGACAC3573741.1981287581913431No Hit
TTCTGT3570001.1968748892597376No Hit
TCGGAC3495361.1718511520792483No Hit
AACTCT3477141.1657427317760796No Hit
GGACTA3419081.1462775900196593No Hit
CAGCCT3412821.1441788682250469No Hit
CTGAGG3402511.1407223471863164No Hit
TTCCAT3375441.131646884090445No Hit
CTGTAT3353861.124411993303267No Hit
ATACCT3353241.1242041326782417No Hit
TTACGT3334831.1180320131512747No Hit
TTCGTT3320231.1131372306909968No Hit
ATCCAC3304081.107722796668155No Hit
TGATAA3295711.1049166782303108No Hit
TACAGT3261381.0934072342672054No Hit
GGCAGG3259371.0927333635312355No Hit
AGTCAA3246541.088431989629498No Hit
CGTCAG3197941.0721383987000799No Hit
AACTAG3169641.0626505669448836No Hit
CTTCAC3138651.0522608882843347No Hit
TACGGC3107091.0416801119523917No Hit
AATCGG3081271.0330237226972974No Hit
CACATT2991111.0027967647097182No Hit
AGGACT2987981.0017474038124121No Hit
GTCTGC2987681.0016468260906253No Hit
ACGTAA2973490.9968894998501225No Hit
CATTAA2972740.9966380555456561No Hit
AGTGCA2968870.9953406029346098No Hit
GTGGTG2958640.991910902621689No Hit
GCATAA2942390.9864629426915852No Hit
TTGAAT2907640.9748126899179785No Hit
ACAGCC2863320.9599540078193814No Hit
GAGCAC2839780.9520620092498648No Hit
CAACGG2822220.9461748599346265No Hit
GAGAAG2814630.9436302435734272No Hit
AGTTAG2806300.9408375354984878No Hit
GGAGGC2780870.932311897281716No Hit
TACGAA2770170.928724625204663No Hit
AGTAGG2701140.9055816914215818No Hit
GAGTCG2687400.9009752317637587No Hit
CTCGTC2667440.8942834606742281No Hit
CGTGAA2654100.8898111046454537No Hit
AAGGCG2634630.8832836105015076No Hit
CCACTT2615560.8768902199866104No Hit
TTCTAC2598940.8713182141996366No Hit
ATTGAA2563580.8594634533917305No Hit
CGCTTG2504270.839579237794533No Hit
TCATGT2466840.8270304907063No Hit
ATCAGC2458230.8241439100910266No Hit
GACCAT2412040.8086582935266268No Hit
CGTTGC2404980.8062913644739169No Hit
GCTTCT2404580.8061572608448684No Hit
GAGTGA2327960.7804697107005878No Hit
TCTCTG2299150.7708108968183546No Hit
CCGTCG2292990.7687457009310044No Hit
CAGTCC2288780.7673342602352667No Hit
TCAATG2279120.7640956575937403No Hit
GTACGC2225740.74619952829719No Hit
ACGCTA2220410.744412597440116No Hit
ACTAAT2214370.7423876326414804No Hit
CTAGTT2089980.7006847565980577No Hit
GACTTG2077870.696624769228608No Hit
ACTAGC1990240.6672460166947619No Hit
GCCATA1823040.6111906997524011No Hit
AAGTTG1814680.608387933905283No Hit
AATGTA1813460.6079789178366846No Hit
GACCGA1739480.5831764516441256No Hit
TGTGTG1714300.5747346281955092No Hit
TATAAG1679730.5631447220549746No Hit
ATTCAT1639950.5498081161460803No Hit
GAACCG1503580.5040888364126488No Hit
CGACAA1376440.46146399791951626No Hit
GCCTGG1318780.44213295979214473No Hit
CGAAGC1235870.4143366300810734No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)