FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26929191
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGA4408991.637253046331767No Hit
GTACTT4275451.5876637363521244No Hit
TATTCT4119961.529923420276532No Hit
TGTGAT4113591.5275579574596208No Hit
GATTCA4110411.5263770827723713No Hit
CCTGAC4044881.502042894641729No Hit
GTCTCA3974971.4760822187343097No Hit
AATTGC3934781.4611578936775338No Hit
GTTACA3911201.4524015964683084No Hit
CAAGGA3767371.398991154245963No Hit
AGTTCC3744691.3905690668538835No Hit
ATTGCT3648621.3548940255947532No Hit
ACCGCT3646861.3542404597301123No Hit
CCTCGG3612851.3416110420844058No Hit
TAGAGC3575401.327704200248719No Hit
CTGGAG3571521.3262633845925784No Hit
CGAATT3454551.2828272486908352No Hit
TATGGT3444281.279013543332958No Hit
GGATTC3419391.2697707851676643No Hit
ATTCGA3415931.2684859340928585No Hit
GAGATA3403371.2638218504224652No Hit
TTAGCG3371031.2518125776596853No Hit
TTCTGT3212691.1930139304964638No Hit
TGACAC3185081.1827611159949067No Hit
AACTCT3132691.1633063911945962No Hit
GGACTA3126601.1610449047652416No Hit
TCGGAC3117551.1576842393817177No Hit
CTGAGG3104691.1529087524389425No Hit
CAGCCT3072211.1408474914823843No Hit
CTGTAT3058621.1358009232434796No Hit
TTCCAT3038961.1285002954600456No Hit
TTACGT3038621.1283740384180125No Hit
TTCGTT3006691.1165170167941547No Hit
TGATAA3000691.1142889513465146No Hit
ATACCT2997081.112948398635518No Hit
ATCCAC2973791.1042997912562615No Hit
AGTCAA2953301.0966909477525708No Hit
GGCAGG2951721.096104223851359No Hit
TACAGT2941511.092312799147958No Hit
CGTCAG2913831.0820339905495118No Hit
AACTAG2895261.075138127989066No Hit
AATCGG2803321.0409967384463945No Hit
CTTCAC2792781.0370827701433736No Hit
TACGGC2777611.0314494780032568No Hit
GTGGTG2737581.0165845680250847No Hit
AGGACT2721031.0104388208320108No Hit
CACATT2699181.0023249491601882No Hit
GTCTGC2690020.9989234359101244No Hit
AGTGCA2684910.9970258668372176No Hit
ACGTAA2677550.9942927732214458No Hit
CATTAA2666090.9900371682164533No Hit
TTGAAT2659450.9875714424543982No Hit
GCATAA2623260.974132494362716No Hit
GAGAAG2603880.9669358429668385No Hit
AGTTAG2599240.9652128056873303No Hit
GAGCAC2573460.9556395511473033No Hit
CAACGG2563900.9520895002007302No Hit
ACAGCC2562110.9514247940088508No Hit
GGAGGC2507990.9313276436711374No Hit
GAGTCG2482870.921999476330351No Hit
TACGAA2475530.9192738095994046No Hit
AGTAGG2470280.9173242523327195No Hit
AAGGCG2424460.9003092591975749No Hit
CGTGAA2405070.8931088943592846No Hit
ATTGAA2374160.8816306438615257No Hit
CCACTT2365920.8785707673134332No Hit
CTCGTC2365810.8785299194468932No Hit
TTCTAC2361640.8769814139607833No Hit
CGCTTG2276490.8453614518163579No Hit
TCATGT2222400.8252754418058826No Hit
ATCAGC2191690.8138714601563783No Hit
GCTTCT2188950.8128539769352893No Hit
CGTTGC2164980.8039528554719672No Hit
GACCAT2153260.7996007009642435No Hit
GAGTGA2123850.7886794668283945No Hit
CCGTCG2096850.7786531723140142No Hit
TCTCTG2080190.7724665772544003No Hit
TCAATG2074200.770242225249173No Hit
CAGTCC2046450.7599374225538376No Hit
ACTAAT2001300.743171230060346No Hit
GTACGC1993660.7403341600570177No Hit
ACGCTA1987500.7380466795307739No Hit
GACTTG1916290.711603256109699No Hit
CTAGTT1855230.6889289767375485No Hit
ACTAGC1783880.6624335651226952No Hit
AATGTA1651470.6132638741356916No Hit
AAGTTG1649410.6124989049986685No Hit
GCCATA1648240.6120644322363787No Hit
TGTGTG1573010.584128204965385No Hit
GACCGA1563080.5804407566495406No Hit
TATAAG1532960.5692558681023875No Hit
ATTCAT1483090.5507369307900858No Hit
GAACCG1364250.5066063811571614No Hit
CGACAA1228430.45617040630741557No Hit
GCCTGG1202960.4467122684821835No Hit
CGAAGC1120380.41604666103783067No Hit
NNNNNN781700.29027979340337406No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)