FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28757096
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGA4729001.6444636829810633No Hit
GTACTT4596331.5983289828708713No Hit
TATTCT4434761.5421445892867625No Hit
GATTCA4419291.5367650474860188No Hit
TGTGAT4411721.534132653728318No Hit
CCTGAC4348431.5121241727606987No Hit
GTCTCA4262181.4821315754553241No Hit
AATTGC4219601.4673247952435808No Hit
GTTACA4162721.447545329333671No Hit
CAAGGA4078921.4184046956618985No Hit
AGTTCC4020101.3979506136502795No Hit
ACCGCT3945981.372176105681881No Hit
ATTGCT3928731.366177586220806No Hit
CCTCGG3878771.3488044829004988No Hit
TAGAGC3828291.3312505546457125No Hit
CTGGAG3790731.3181894305322068No Hit
CGAATT3726321.2957914804749409No Hit
TATGGT3694481.2847194306407017No Hit
GGATTC3676591.2784983574141144No Hit
ATTCGA3675791.278220165207224No Hit
GAGATA3648551.2687477205626048No Hit
TTAGCG3585151.246700988166538No Hit
TTCTGT3446801.1985911233874242No Hit
TGACAC3426771.191625886007405No Hit
AACTCT3394761.1804947203292016No Hit
TCGGAC3355781.1669398050484652No Hit
GGACTA3354401.1664599234915793No Hit
CTGAGG3325431.1563858881995595No Hit
CAGCCT3317381.1535865791177247No Hit
CTGTAT3290941.1443923266799958No Hit
TTCCAT3269681.1369993687818827No Hit
TTACGT3258851.1332333417811034No Hit
ATACCT3248491.1296307527018723No Hit
TGATAA3212491.117112103391803No Hit
TTCGTT3206201.114924817165127No Hit
ATCCAC3201241.1132000254824062No Hit
AGTCAA3171781.102955597463666No Hit
TACAGT3170001.1023366198033349No Hit
GGCAGG3143841.0932397346380176No Hit
AACTAG3104421.0795318136434917No Hit
CGTCAG3099781.0779182988435272No Hit
CTTCAC3016991.0491288828329537No Hit
AATCGG3000761.043485058435664No Hit
AGGACT2952451.0266857265420681No Hit
TACGGC2947491.0249609348593474No Hit
GTCTGC2912331.0127343873665131No Hit
GTGGTG2903111.0095282221821007No Hit
CACATT2890341.005087579079612No Hit
ACGTAA2871830.9986509068926848No Hit
AGTGCA2870980.9983553276728637No Hit
TTGAAT2870650.9982405733875214No Hit
CATTAA2863340.9956985920970601No Hit
GCATAA2814240.9786245453991599No Hit
GAGAAG2779180.9664327719321868No Hit
ACAGCC2776960.9656607885580658No Hit
GAGCAC2771720.9638386296029335No Hit
AGTTAG2770570.9634387283055286No Hit
CAACGG2738670.9523458140557726No Hit
GAGTCG2653670.9227878920736642No Hit
GGAGGC2653160.9226105445417715No Hit
TACGAA2651190.9219254962323039No Hit
AGTAGG2639740.9179438702711845No Hit
CGTGAA2584070.8985851700741966No Hit
AAGGCG2574690.8953233664484063No Hit
CCACTT2558630.8897386578950809No Hit
CTCGTC2558110.8895578329606021No Hit
TTCTAC2555240.8885598184183827No Hit
ATTGAA2554340.8882468521856309No Hit
CGCTTG2436120.8471369988123975No Hit
TCATGT2406020.836670017028145No Hit
ATCAGC2354800.8188587609819852No Hit
GCTTCT2349870.8171444015070228No Hit
CGTTGC2342000.8144076856717383No Hit
GACCAT2313310.8044310176521302No Hit
GAGTGA2265480.7877986010826685No Hit
CCGTCG2242480.7798005751345686No Hit
CAGTCC2229110.7751512878769122No Hit
TCTCTG2226990.7744140785286525No Hit
TCAATG2219070.7716599756804373No Hit
ACTAAT2166520.753386225090322No Hit
GTACGC2135900.7427384183715907No Hit
ACGCTA2133960.7420638022698816No Hit
GACTTG2041690.7099778086076564No Hit
CTAGTT1998350.6949067457993672No Hit
ACTAGC1929530.6709752612016179No Hit
GCCATA1778440.6184351855277738No Hit
AATGTA1763680.6133025393106453No Hit
AAGTTG1763030.6130765081425468No Hit
GACCGA1678720.5837585269388814No Hit
TGTGTG1661800.5778747617631488No Hit
TATAAG1624460.564890140506538No Hit
ATTCAT1616660.5621777664893562No Hit
GAACCG1461960.508382348481919No Hit
CGACAA1318670.45855464682525665No Hit
GCCTGG1282640.4460255653074288No Hit
CGAAGC1202220.41806029370976816No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)