FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28369826
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACT3956661.3946719306632336No Hit
GGCTAC3537871.247053824017109No Hit
CTTAGC3162621.1147830092436943No Hit
GTACTT2942181.0370807350034505No Hit
TCATGT2871851.012290311544385No Hit
CTGTTC2661960.9383067770665918No Hit
TGTGAT2585090.9112110874419885No Hit
CCGTCG2555980.9009501855950756No Hit
GGTAAT2529790.8917185463174854No Hit
GGCCGT2431390.857033807680033No Hit
TAGCTT2394980.844199749409813No Hit
CTTGTA2292580.808105062047261No Hit
GCTCAA2245680.7915734132454673No Hit
CCTTAT2210140.7790460188229564No Hit
AAGCGC2131860.7514533222727555No Hit
ATACCT2122940.7483091366157832No Hit
CAGTAG2113400.7449464089064205No Hit
TTCTAC2102480.7410972488868983No Hit
GTCGAG2099850.7401702076001453No Hit
GTCTGC2085030.734946347573651No Hit
CGAAGC2057330.7251824526523356No Hit
AGTTAG2057080.7250943308570169No Hit
GATCAG2054320.724121466236698No Hit
TCTCTG2053390.7237936531581124No Hit
ACTAAT2042650.7200079408312198No Hit
TTGAAT2028570.7150449213188689No Hit
GGCGTT2025640.7140121338777333No Hit
AGACGG1976510.6966944386616964No Hit
CCATGA1971080.6947804332673736No Hit
GAATAC1960090.6909065991451622No Hit
AACATA1940450.6839837509049227No Hit
CTGAGG1900360.6698525398076111No Hit
ACTAGC1900280.669824340833109No Hit
GGCCAG1891870.666859923638587No Hit
CTGTAT1869250.6588866635981483No Hit
ACTTGA1865770.6576600082073115No Hit
TATAAG1832360.6458834114809164No Hit
GTAAGA1828500.6445228109611952No Hit
ATGCCG1828070.644371241473247No Hit
TGCGGA1798990.6341209142417722No Hit
AGTCAA1789170.6306594901216525No Hit
AGCCTA1785230.6292706906274292No Hit
CAACGG1772030.6246178598346004No Hit
AACTCT1768850.623496950598146No Hit
TTCTGT1746050.6154602428650778No Hit
ATTCAT1713470.6039762104991409No Hit
GCATAA1712560.6036554471641806No Hit
AACCTT1712200.6035285517789217No Hit
TGATAA1711270.6032007387003361No Hit
TACGGC1708070.6020727797202563No Hit
GTCTCA1705590.6011986115106945No Hit
AAGCAT1695430.5976173417489413No Hit
CCTGCT1691640.5962814153319093No Hit
CTACCG1676540.590958858894658No Hit
CCAGCG1672790.589637031964877No Hit
CATAAC1672250.5894466888869886No Hit
TACGAA1665450.5870497760543192No Hit
TTAGGC1662700.5860804363058131No Hit
ATTGAA1651670.5821925026963507No Hit
GTACGC1648230.580979946792765No Hit
TCAATG1647380.5806803326886812No Hit
TCGGAC1634440.5761191485629839No Hit
GACACG1619190.5707437190485413No Hit
TCAAGC1609290.5672540959539195No Hit
CTTCAC1595080.5622452531080028No Hit
TTACGT1572700.5543565899910701No Hit
GAGATA1557400.5489635361175638No Hit
ACGAAC1556570.5486709717571056No Hit
GTTACA1530910.539626150685591No Hit
AGATGC1526200.5379659360617862No Hit
ATCAGC1524140.53723981246836No Hit
CACATT1522120.5365277883621845No Hit
TAATGA1518600.5352870334840968No Hit
GGACTA1503350.5299116039696542No Hit
TACTCG1495740.5272291765201521No Hit
GATTCA1492250.5259989962575027No Hit
CGTTGC1485760.5237113544510283No Hit
TGACCA1479950.5216634039278211No Hit
ATCCGG1467990.5174476572397729No Hit
AAGTTG1466030.5167567823644742No Hit
TCTTCA1462780.5156111990253306No Hit
CCGACT1458030.5139368849142747No Hit
GCCAAT1457560.5137712159390756No Hit
ATGGCC1456260.5133129826034182No Hit
CGAATT1451250.5115470218252308No Hit
GTTGGC1444650.5092206064288164No Hit
CATACG1443140.5086883507850912No Hit
ACAGCC1442870.508593179246147No Hit
GAGCAC1440970.5079234536017245No Hit
ACCAGG1437230.5066051515437564No Hit
TGCATA1434740.5057274584623819No Hit
CAGTCC1427800.5032811974243339No Hit
TTCACA1427320.5031120035773219No Hit
CATGAG1418260.49991846971497117No Hit
GAGAAG1400010.49348557865670384No Hit
TGTGTG1394000.4913671306972415No Hit
CAAGGA1378430.485878905284791No Hit
AACTAG1376480.48519155528130486No Hit
TGACAC1374020.4843244368153686No Hit
GTGGTG1365230.481226074491962No Hit
TCCAGA1362490.4802602596152687No Hit
AAGAGG1358450.47883621140291804No Hit
GAACCG1347870.4751068970250293No Hit
TTCCAT1341310.4727945811158658No Hit
ACGCTA1325060.4670666644201483No Hit
ATTCGA1324080.4667212269824989No Hit
AATGTA1322750.4662524190314033No Hit
ACAAGT1311490.46228341337024764No Hit
CTAGTT1309250.46149384208419186No Hit
AGGTGT1296480.4569925807793111No Hit
GCCTGG1283510.45242082203817535No Hit
TGGCAT1282450.452047185626024No Hit
AGGACT1281130.451581902546741No Hit
GCGCAG1279210.4509051271586932No Hit
ACCGCT1276060.44979479253767723No Hit
CCGGAA1273700.4489629227898684No Hit
CCGCTC1263890.44550502354156135No Hit
GACCGA1263820.4454803494388721No Hit
CTCGTC1257800.44335837660759714No Hit
GACCAT1254750.44228329070470856No Hit
CAGATC1254010.44202245019056513No Hit
TAAGTC1252610.4415289681367803No Hit
CGTCAG1251800.4412434535199476No Hit
GACTAA1244270.4385892250449474No Hit
TCCTCC1241930.4377644050407641No Hit
GCTTCT1239390.4368690876003257No Hit
GCTGTC1239260.43682326426676005No Hit
GCCATA1227170.43256169424514623No Hit
ACAGTG1226200.4322197816793096No Hit
ATGTGA1220910.4303551244903652No Hit
CCTCGG1215700.42851866627592283No Hit
CCACTT1180400.41607586877691816No Hit
GCGGCT1163010.4099461166945473No Hit
CGTGAA1161190.4093045900246269No Hit
ATTGCT1157730.40808498437741564No Hit
TTAGTA1157490.4080003874539097No Hit
TCGTGG1156010.4074787064256228No Hit
CAGCCT1155910.4074434577074953No Hit
GCACAT1150410.40550477821048325No Hit
GCGTAT1138050.40114803664992515No Hit
TGCGAC1125740.3968089194484309No Hit
GACTTG1124220.396273138932893No Hit
CAAGCT1111720.3918670491669564No Hit
TATGGT1102140.3884902219703427No Hit
AATCGG1101500.3882646301743268No Hit
CACCTA1091190.38463048733538235No Hit
CATTAA1085550.38264245963299176No Hit
TAGAGC1079760.38060155885340996No Hit
AGGCTT1068110.3764950831915571No Hit
ACCTGC1044540.3681869603289072No Hit
AACAAT1033720.36437304902751255No Hit
TATTCT1032500.36394301466635715No Hit
CTATGC1025480.36146855465380723No Hit
TTAGCG1024700.36119361465241273No Hit
CTGGAG1021340.360009257723329No Hit
CGACAA1017610.3586944805371735No Hit
GGTGGT1005200.35432011461755175No Hit
GAGTGA1001120.3528819669179501No Hit
AGTAGG1000340.3526070269165556No Hit
CGTCTC979850.3453845645722325No Hit
AGAACG977560.3445773689271129No Hit
TTCGTT969660.34179272019504103No Hit
ACGTAA956800.33725973504384554No Hit
AGTGCA942290.3321451460435464No Hit
AATTGC940970.3316798629642635No Hit
CTCCGC922740.3252540216496217No Hit
CGATGT896050.315846138781394No Hit
AAGGCG893710.3150213187772107No Hit
TACAGT877040.30914535746535776No Hit
TCTCGC868890.3062725869379671No Hit
GAGTCG821350.28951534634015735No Hit
AGGTCA815300.28738279889344404No Hit
GGCAGG805550.28394604887601355No Hit
GGAGGC800010.2819932698917505No Hit
ATCCAC796290.2806820175774078No Hit
CGCTTG746280.26305413364184893No Hit
GGATTC713120.2513656587107725No Hit
AGTTCC651260.22956080167710582No Hit
CCTGAC622910.21956779008796173No Hit
NNNNNN530730.18707552171803943No Hit
AGAGGT454970.16037109286465134No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)