FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26692609
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACT5795892.1713463828133097No Hit
AACATA5366002.010294310308895No Hit
TGCGGA4824531.8074404041957832No Hit
GACACG4493641.6834772502006081No Hit
GTTGGC3993261.4960171184465334No Hit
ACCAGG3954811.4816123819144094No Hit
CCATGA3935831.4745017993557692No Hit
CATGAG3927971.4715571640074598No Hit
CCGCTC3426511.283692425869648No Hit
TCCTCC3413991.279001988902621No Hit
GACTAA3413331.2787547294458927No Hit
GCTGTC3409741.277409787855507No Hit
CCTGCT3376341.264896960802895No Hit
CATAAC3357151.2577077047807503No Hit
GCTCAA3265281.2232899376752568No Hit
GCGTAT3123791.170282755050284No Hit
GCGGCT3102601.1623442279471445No Hit
AGATGC3065301.1483703222865924No Hit
TTGAAT2945711.1035676579985119No Hit
TCTCTG2923461.0952320172224452No Hit
AAGAGG2779001.0411121670421952No Hit
GGCTAC2526170.9463930633382446No Hit
CTTAGC2471700.925986665447353No Hit
CTACCG2450240.9179469867482793No Hit
CCAGCG2437260.9130842174326235No Hit
TTAGTA2404080.9006538101989207No Hit
GTACGC2337610.8757517858220604No Hit
GCACAT2330110.8729420192683299No Hit
TCGTGG2307610.8645127196071393No Hit
TTACGT2263360.8479350969401306No Hit
CAAGCT2244820.8409893540193093No Hit
CACCTA2220440.8318557395419833No Hit
CCGTCG2209120.827614865223553No Hit
AGGCTT2179890.8166642683748149No Hit
TCTTCA2136190.8002926952550797No Hit
GTACTT2120050.7942460776314522No Hit
TTCACA2116230.7928149698667522No Hit
ACCTGC2114980.7923466754411305No Hit
GGTAAT2073030.7766307145172657No Hit
GAGAAG2036460.7629302928012769No Hit
GCGCAG1978450.7411976850970244No Hit
GGCCGT1935890.7252531964934563No Hit
ACGCTA1911560.7161383137931553No Hit
CCGGAA1849910.6930420327214923No Hit
GTAAGA1831780.6862498903722749No Hit
AGTTAG1827130.6845078351089622No Hit
ATGTGA1822150.6826421501172852No Hit
CTCGTC1814660.6798361299189599No Hit
CGTCAG1805260.6763145558382847No Hit
AGTCAA1784870.6686757371675432No Hit
CGAAGC1769750.663011247795223No Hit
TAGCTT1729690.6480033480428984No Hit
CGTGAA1684030.6308974892637883No Hit
GTCGAG1673830.6270762067507152No Hit
AAGCGC1660920.622239661922894No Hit
CAGCCT1657600.6209958719284427No Hit
TGCGAC1637970.6136417762684794No Hit
CTTGTA1637810.6135818345819998No Hit
ATACCT1607910.6023802319211284No Hit
GAGATA1585820.5941045328315414No Hit
GTCTGC1570550.5883838481281467No Hit
ACTAAT1545370.5789505252184228No Hit
CACATT1544890.578770700158984No Hit
GGACTA1516880.5682771586696527No Hit
GATCAG1489440.5579971594384049No Hit
AGAACG1465630.5490770872191625No Hit
CGAATT1456800.5457690553965706No Hit
TACGAA1443500.5407864027079555No Hit
CTGTAT1440850.5397936185256376No Hit
TCGGAC1416080.5305138961875177No Hit
TATAAG1403090.5256473805164568No Hit
ACTTGA1337210.5009663911084901No Hit
TTCTGT1328850.49783443798993193No Hit
ATTCAT1325300.49650448182116635No Hit
TGATAA1309310.4905140595286133No Hit
AAGGCG1304740.48880197510854034No Hit
ATTGAA1273930.4772594541058163No Hit
AACTCT1269500.47559981866141293No Hit
ACCGCT1267310.47477936682772376No Hit
CAGTCC1233230.4620117876075733No Hit
AGGTCA1230590.46102274978066027No Hit
GTCTCA1214670.4550585519759421No Hit
TACTCG1197960.44879839209423106No Hit
CAACGG1192870.4468914971930994No Hit
GAACCG1191580.4464082173458578No Hit
TTCCAT1188300.4451794127730264No Hit
TTAGGC1184930.44391689100155024No Hit
ATCAGC1152750.43186111930834487No Hit
GCATAA1136570.4257995162630974No Hit
GGAGGC1129570.4231770674796158No Hit
CTTCAC1116140.4181457121707361No Hit
AATCGG1107670.4149725491427234No Hit
TACGGC1080410.404759984308765No Hit
GCCATA1079050.40425047997368857No Hit
TCCAGA1079040.4042467336182836No Hit
CTGTTC1069880.40081507206732775No Hit
AGGTGT1055800.3955402036571247No Hit
TGACCA1054660.39511311914095776No Hit
GTTACA1044400.3912693584954547No Hit
TATTCT1040860.389943148682094No Hit
GCCAAT1039170.38931001461865344No Hit
ACTAGC1031460.3864215746014187No Hit
TCATGT1026260.38447346979083236No Hit
CTGGAG1018910.3817198985681767No Hit
CTAGTT996370.3732756134853659No Hit
GACTTG993530.3722116485503534No Hit
CAAGGA977430.3661800163483457No Hit
TGACAC977060.3660414011983617No Hit
ACAGCC962070.3604256144463061No Hit
GACCAT957870.35885214517621716No Hit
TGTGAT943020.35328880739983115No Hit
ACAAGT939670.352033778339165No Hit
AACTAG927370.3474257611910473No Hit
TCAATG910280.3410232398039472No Hit
CAGATC903330.3384195227974905No Hit
CGACAA889330.3331746252305273No Hit
ACAGTG881420.3302112581051931No Hit
AGTGCA831950.3116780379167881No Hit
AAGTTG817600.3063020179106508No Hit
CGTTGC816880.30603228032149277No Hit
CCTCGG815220.3054103853242671No Hit
ATCCAC806620.3021885196759897No Hit
GAGCAC801380.3002254294437835No Hit
CGTCTC776750.2909981560813332No Hit
CTCCGC772360.28935350605854976No Hit
TTCTAC767560.2875552554641624No Hit
TGTGTG767330.287469089289848No Hit
TAGAGC762130.28552098447926166No Hit
TATGGT747860.28017493531636417No Hit
GGATTC743250.2784478654746713No Hit
AATGTA739060.27687814255998733No Hit
TTAGCG735320.2754770056385271No Hit
AAGCAT733760.27489257419535124No Hit
AGGACT717440.26877852217443415No Hit
AACCTT709180.26568403260992585No Hit
TCTCGC701000.26261951388865734No Hit
GACCGA695830.26068264814428593No Hit
GCTTCT695460.2605440329943019No Hit
AGTAGG692430.25940888730659484No Hit
GAGTGA688940.25810140927025904No Hit
CTGAGG686760.25728470379197477No Hit
TCAAGC666750.24978824662662238No Hit
TAATGA658630.2467462060377837No Hit
ACGTAA655870.24571221194601098No Hit
CGATGT639070.23941833486565514No Hit
ACGAAC638580.23923476345081143No Hit
TACAGT628620.23550339346745758No Hit
ATCCGG608290.22788705292914607No Hit
CATTAA602570.2257441376375011No Hit
TGCATA585070.2191880156787971No Hit
GGCAGG574780.2153330159670791No Hit
CATACG566620.2122759899566206No Hit
GAGTCG560690.21005440120147115No Hit
TAAGTC547100.2049631042061119No Hit
GATTCA546490.2047345765264085No Hit
TTCGTT539430.20208964961049702No Hit
TGGCAT531790.19922743408109714No Hit
GTGGTG499080.18697310555142813No Hit
CCTTAT484860.1816457881655555No Hit
ATTCGA480260.1799224646792676No Hit
AGTTCC474760.17786196920653202No Hit
GCCTGG465050.17422425810830255No Hit
CAGTAG460880.17266202790442853No Hit
CTATGC449250.1683050165684441No Hit
CCTGAC448080.1678666929860622No Hit
GGCGTT438600.1643151480621471No Hit
CCACTT431520.1616627284354257No Hit
ATTGCT430690.16135178093681288No Hit
GAATAC425820.15952730585459068No Hit
AGACGG418340.15672503201167035No Hit
GGCCAG413460.15489681057404317No Hit
ATGCCG400940.15020637360701608No Hit
AGCCTA383850.143803852219916No Hit
AGAGGT378620.14184450834311477No Hit
AATTGC341430.12791181259201753No Hit
CCGACT318090.11916781907680887No Hit
ATGGCC314030.11764679878238954No Hit
CGCTTG269110.1008181703032476No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)