FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19769409
Sequences flagged as poor quality0
Sequence length6
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAG4591752.32265415723859No Hit
TTCCAT4083522.0655751519936687No Hit
GAGATA3874091.959638752984472No Hit
GGTACC3732781.8881596308721216No Hit
TTACGT3589871.815871177534948No Hit
AAGTTG3481021.7608113626461974No Hit
CTATTA3441121.7406286652271699No Hit
AACCTT3381961.7107036431893337No Hit
TTGAAT3331831.6853462842515927No Hit
CAGCCT3303201.6708643136474137No Hit
AATCGG3265021.6515516472950706No Hit
GATCCT3242561.6401906602266159No Hit
TAATGA3172201.6046003196150171No Hit
CAGTCC3167591.6022684340234956No Hit
AGTTAG3093781.5649329729583723No Hit
CGAATT2963791.4991798692616456No Hit
ATACCT2947091.4907324746025539No Hit
AGTCAA2870691.4520869086172479No Hit
TATTGG2844461.4388189348503033No Hit
GACCAT2827661.4303209569896602No Hit
TCAAGC2710071.370840170285313No Hit
GTACGC2705771.3686650926186008No Hit
GCTTCT2691451.3614215781564334No Hit
CGTGAA2598821.3145663585593277No Hit
TCGGAC2529581.2795425498051054No Hit
AAGGCG2528381.278935551386488No Hit
GGATCG2492971.2610240397171206No Hit
GTCTGC2486821.2579131728217066No Hit
ACTAAT2482921.2559404279612No Hit
CGAGCC2445201.236860444335994No Hit
CGACAA2417631.22291465566826No Hit
GAGCCA2390061.2089688670005259No Hit
GTAAGA2385061.2064397069229535No Hit
ACCGCT2366111.1968541902289542No Hit
CAGGAC2345841.186600975274476No Hit
CATTAA2315921.1714664813702826No Hit
AAGCAT2315671.1713400233664042No Hit
GAACCG2262541.14446516838212No Hit
ACACGA2219831.122861082999497No Hit
GGATTC2212211.1190066430412764No Hit
AGTGCA2201621.1136498819969782No Hit
GAAGAT2180521.102976826469623No Hit
CTGGAG2179841.102632860699073No Hit
TATTCT2119431.0720755486418436No Hit
ATCAGC2116691.0706895689193339No Hit
CACATT2100361.0624293321059826No Hit
ACTAGC2058001.0410022879287895No Hit
CTGTTC2028821.026242109716077No Hit
TAAGTC1956240.9895288220300363No Hit
CTCGTC1952480.9876268936517019No Hit
GGACTA1951060.9869086121896714No Hit
ATCCGG1950130.986438188415243No Hit
CTAGTT1948240.9854821659059206No Hit
TCCACG1946470.9845868432384599No Hit
TGTGTG1931740.9771359376499318No Hit
TGCATA1922080.972249600380062No Hit
ATTGAA1916530.9694422326939567No Hit
ATCCAC1914320.9683243439396696No Hit
ACGCTA1898050.960094457047249No Hit
GCCATA1871700.9467657834384426No Hit
CTGTAT1868780.9452887539531404No Hit
AGGACT1865970.9438673659895447No Hit
TACGAA1862210.9419654376112103No Hit
CTATGC1782320.9015545178917589No Hit
CATACG1768240.8944324031133152No Hit
TTCTGT1725740.8729345424539501No Hit
GACTTG1681600.850607117289141No Hit
CGAGAG1653130.8362060798074439No Hit
TCAATG1612360.8155833085349187No Hit
AATGTA1593510.8060483750424708No Hit
CCGTCG1587640.803079141111401No Hit
CGTTGC1546150.7820921707877053No Hit
TGATAA1519800.768763497178899No Hit
CGTCAG1322990.6692106982054952No Hit
TTCGTT1319980.6676881438387966No Hit
TGGCAT1283150.6490583507073985No Hit
ATTCAT1225450.6198718434122132No Hit
GGAGGC1221210.6177271156664319No Hit
GACCGA1207260.610670759050005No Hit
GAGCAC1130520.5718532101794241No Hit
ACGAAC1119300.5661777749653518No Hit
TATAAG989280.5004094963081598No Hit
CGAAGC974380.4928725992769941No Hit
TCTCTG913180.4619156799275082No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)