FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27070858
Sequences flagged as poor quality0
Sequence length6
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAG6258592.3119289384917168No Hit
TTCCAT5551382.050684909950028No Hit
GAGATA5323621.9665501551520828No Hit
GGTACC5144241.9002870171311157No Hit
TTACGT4890881.8066955986396884No Hit
AAGTTG4721631.744174491994306No Hit
CTATTA4702391.7370672181871736No Hit
AACCTT4626831.7091552842543816No Hit
CAGCCT4560181.6845347125680317No Hit
TTGAAT4552951.681863943876474No Hit
AATCGG4429931.636420242018188No Hit
GATCCT4420701.6330106714755772No Hit
TAATGA4334211.6010611854267789No Hit
CAGTCC4330561.5997128720486067No Hit
AGTTAG4203161.5526511941365138No Hit
CGAATT4055891.498249519834207No Hit
ATACCT4036261.4909981796661191No Hit
AGTCAA3914851.446149213297931No Hit
TATTGG3879971.433264509015562No Hit
GACCAT3845771.4206309973625513No Hit
TCAAGC3703031.36790270925288No Hit
GTACGC3690301.3632002354709258No Hit
GCTTCT3656181.3505962758919572No Hit
CGTGAA3600371.329980010238316No Hit
TCGGAC3474841.2836091120569582No Hit
AAGGCG3459831.2780644041648033No Hit
GTCTGC3395591.2543340887089727No Hit
ACTAAT3379461.248375651780228No Hit
GGATCG3359101.2408546489365058No Hit
CGAGCC3353991.2389670102070647No Hit
CGACAA3296881.217870523350239No Hit
GTAAGA3274101.2094555702667422No Hit
GAGCCA3249701.2004421876838924No Hit
CAGGAC3221861.190158065917231No Hit
ACCGCT3215221.1877052437717341No Hit
AAGCAT3170271.1711006721693122No Hit
CATTAA3161581.1678905781264857No Hit
GAACCG3080511.1379432450940417No Hit
GAAGAT3045801.1251213389690125No Hit
ACACGA3040841.1232891103784002No Hit
AGTGCA3020351.1157200854143596No Hit
GGATTC3015911.1140799453050214No Hit
CTGGAG2964131.0949523653812525No Hit
CACATT2919041.0782960776492565No Hit
TATTCT2895811.069714894149273No Hit
ATCAGC2877331.062888365045541No Hit
ACTAGC2813041.0391395795434337No Hit
CTGTTC2812151.038810812719715No Hit
GGACTA2719891.0047298833306282No Hit
TAAGTC2682230.9908182444752951No Hit
TCCACG2679640.9898614960781812No Hit
CTCGTC2666040.984837643491019No Hit
ATCCGG2658990.9822333669660562No Hit
CTAGTT2648090.9782068968778159No Hit
ATTGAA2626340.9701724267476117No Hit
TGCATA2618910.9674277778709489No Hit
TGTGTG2612280.9649786497347074No Hit
ATCCAC2600030.9604534883970062No Hit
ACGCTA2596610.959190137231705No Hit
TACGAA2560870.9459877481533832No Hit
AGGACT2545380.9402657278169758No Hit
GCCATA2542990.9393828596049671No Hit
CTGTAT2520450.9310565627435967No Hit
CTATGC2436930.9002041974436126No Hit
CATACG2400210.8866397954582748No Hit
TTCTGT2360610.8720115188074201No Hit
GACTTG2281740.8428768678111348No Hit
CGAGAG2246140.8297261948623867No Hit
TCAATG2197440.8117363697892397No Hit
AATGTA2172120.8023831383549054No Hit
CCGTCG2141900.7912198423854907No Hit
CGTTGC2121560.7837062275602791No Hit
TGATAA2081420.7688784744096401No Hit
TTCGTT1804420.6665544180387634No Hit
CGTCAG1803730.6662995314001499No Hit
TGGCAT1745660.6448484196548185No Hit
ATTCAT1668380.6163011161301204No Hit
GGAGGC1655430.6115173741445505No Hit
GACCGA1630320.602241716904577No Hit
ACGAAC1570140.5800111692063842No Hit
GAGCAC1545780.5710125626605554No Hit
TATAAG1336890.49384840332729757No Hit
CGAAGC1321180.4880451147872742No Hit
TCTCTG1244100.4595716914476815No Hit
NNNNNN968140.35763181203935246No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)