FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26721121
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCC4770021.7851122338767151No Hit
TTCTAC4686721.7539383920307836No Hit
AACTAG4562201.7073385506543681No Hit
CCTCGG4411861.6510759410131033No Hit
GAGTGA4150641.553318066259271No Hit
TATGGT3994541.4948998584303406No Hit
CTTCAC3951041.4786206012839058No Hit
GAGTCG3905311.4615067983113432No Hit
TACAGT3862811.4456017769613783No Hit
TACGGC3568001.3352733218041264No Hit
GTTACA3522201.3181333223258112No Hit
AATTGC3413191.2773378781526419No Hit
TGCGAC3363441.2587196472782711No Hit
GTCTCA3346791.2524886212670494No Hit
GTACTT3169091.1859869202343718No Hit
TACTCG3108121.1631697637236103No Hit
ATTCGA3074631.1506366068998377No Hit
TAGAGC3056391.1438105459722292No Hit
CCGGAA2904081.0868106918119191No Hit
CTGAGG2900961.0856430761269333No Hit
GCATAA2873371.0753179105023327No Hit
AGAACG2871571.0746442860686871No Hit
CGTACT2725531.0199908903522423No Hit
GCTCAA2713551.0155075455105345No Hit
TTCACA2696501.009126825180725No Hit
CAACGG2694241.0082810522807033No Hit
ACGTAA2634390.9858830398619878No Hit
GGCCGT2628200.98356652028184No Hit
AGTAGG2584730.9672984902092994No Hit
TCCAGA2543360.9518163553093449No Hit
TCTTCA2525890.9452784559450181No Hit
GATTCA2518150.9423818708803423No Hit
CAAGGA2439930.9131091468804771No Hit
TGACAC2417220.9046102519426487No Hit
AGGTCA2384360.8923128636706521No Hit
TTAGCG2293390.8582686332657975No Hit
CCATGA2281330.8537553495603721No Hit
AACTCT2220430.8309643895553633No Hit
CCAGCG2190070.8196025907745412No Hit
GCCTGG2151550.8051870278945259No Hit
CCACTT2117930.7926052204172123No Hit
TGTGAT2044310.7650539810811081No Hit
CCTGAC1976230.7395760080574464No Hit
GTCGAG1911980.7155313581342639No Hit
GGCAGG1898280.7104043277226281No Hit
TCATGT1888540.7067592710650126No Hit
TCCTCC1885460.7056066248118856No Hit
CACCTA1802080.6744028441022366No Hit
CTCCGC1769170.6620867440404166No Hit
GTGGTG1738750.6507024911118063No Hit
AACATA1690270.632559539698952No Hit
TGCGGA1682040.6294795790940058No Hit
CGCTTG1667620.6240830989089118No Hit
GTTGGC1651090.6178969811932665No Hit
CATGAG1636700.6125117280820667No Hit
GCGTAT1623080.6074146365341484No Hit
CTTAGC1619490.6060711300248219No Hit
ATGTGA1611860.6032157108977576No Hit
AGAGGT1606100.6010601127100917No Hit
GCGCAG1582510.59223189027137No Hit
AGTTCC1577130.5902185016863627No Hit
TTCCAT1575880.5897507069407755No Hit
ATTGCT1569010.5871797070190281No Hit
GCACAT1554050.5815811395038405No Hit
TCTCGC1534820.5743845851377268No Hit
CCTTAT1508700.5646095461339365No Hit
GCGGCT1502360.5622368911843182No Hit
AGATGC1497510.5604218475714399No Hit
ATACCT1489430.5573980223359641No Hit
AGGTGT1481000.554243214571724No Hit
AGCCTA1461010.5467622410002934No Hit
AAGTTG1447740.5417961319811396No Hit
GACCAT1432590.5361264596646226No Hit
GGTAAT1417440.5304567873481056No Hit
CATAAC1414960.5295286825728606No Hit
CCGACT1380200.5165202462875715No Hit
CCTGCT1351100.5056299846103013No Hit
GAATAC1346420.5038785610828229No Hit
AAGCGC1345900.5036839584686585No Hit
GCTGTC1340640.5017154781792276No Hit
GTCTGC1282920.48011458800699264No Hit
TTAGTA1279420.47880476271934846No Hit
CAGTCC1273090.47643585012769485No Hit
GACTAA1272710.4762936405250363No Hit
GGCGTT1271340.4757809374838728No Hit
ACTAAT1261280.4720161253713869No Hit
AGTTAG1234380.4619491824463502No Hit
AAGAGG1226350.45894406900069795No Hit
AGGCTT1219930.4565414751873621No Hit
GAGATA1152720.4313890873066291No Hit
GCTTCT1138830.42619095209366403No Hit
CAGTAG1127620.421995768815238No Hit
CCGCTC1099950.41164066432691954No Hit
ATCAGC1073860.4018768523970233No Hit
ATGGCC1071020.4008140227350492No Hit
ACCTGC1010420.37813533346898137No Hit
ATTGAA987820.36967760446876463No Hit
AACCTT985980.3689890106032602No Hit
GGCCAG983420.36803096696429766No Hit
TCGTGG967880.3622153426871575No Hit
CTAGTT967120.36193092348184047No Hit
CATTAA962010.3600185785618799No Hit
TCGGAC957520.3583382598357307No Hit
AATCGG957170.3582072773069663No Hit
CTGTAT949020.3551572555657377No Hit
CGACAA936470.3504605963200421No Hit
ATGCCG905650.33892664907284387No Hit
TAATGA901500.3373735705174944No Hit
AGACGG899150.3364941163957904No Hit
GAACCG883930.33079824757352055No Hit
CGAATT878110.32862019523806657No Hit
CAAGCT875540.3276584092411392No Hit
CGTCTC870380.32572735253135526No Hit
ACTAGC866780.32438010366406406No Hit
TTCTGT862520.32278585917110286No Hit
AGTCAA838110.3136507633792759No Hit
GACACG830820.3109225844230113No Hit
AGTGCA815470.3051780649472004No Hit
TGTGTG814510.3048187985825894No Hit
GGTGGT804260.3009828816687743No Hit
AGGACT787840.2948379298907407No Hit
TCAAGC783320.2931463840906974No Hit
CTACCG772430.28907095626714163No Hit
TGATAA771060.28855825322597806No Hit
ACCAGG742240.27777277757171936No Hit
GCCATA739190.27663135839248665No Hit
GAGAAG730320.2733118868777998No Hit
GTAAGA706470.2643863631319958No Hit
AACAAT696000.2604681143429574No Hit
TACGAA691620.2588289615544198No Hit
ACCGCT684830.2562879004963901No Hit
TCAATG671260.2512095207382954No Hit
AAGCAT664200.2485674160152188No Hit
CGTTGC655270.24522549035274382No Hit
AATGTA654480.24492984407353272No Hit
GACTTG652380.2441439489009462No Hit
CTGGAG645930.24173012801371618No Hit
GGATTC641190.23995625033844953No Hit
CACATT624920.2338674339298864No Hit
CCGTCG619430.23181287940726739No Hit
TATTCT618760.23156214142363266No Hit
ATTCAT617360.23103821130857496No Hit
CTGTTC590820.2211059932702674No Hit
GGACTA579870.21700811129892342No Hit
TTACGT575120.21523049126569205No Hit
ATCCGG569480.21311980137360256No Hit
ATCCAC558730.20909676656155257No Hit
TGCATA557460.20862148710003597No Hit
TAAGTC555680.20795534738231977No Hit
CATACG543590.2034308366030003No Hit
TTCGTT539010.20171683665516876No Hit
TTGAAT524010.1961032997081223No Hit
CAGCCT521230.19506292419393634No Hit
CTATGC509370.19062448764780487No Hit
TATAAG496410.18577439172555674No Hit
GACCGA494960.18523174982067556No Hit
GGTACC490410.18352897694673811No Hit
GAGCAC474360.17752249241339838No Hit
CTATTA448670.16790837480209006No Hit
GTACGC424780.15896788162442735No Hit
GATCCT421710.15781897772926518No Hit
CGTGAA411160.15387079007650914No Hit
AAGGCG400930.15004235787862344No Hit
CGAAGC388120.1452483973258457No Hit
TGGCAT372010.1392194586447178No Hit
TATTGG369900.13842982111416657No Hit
CGAGCC324990.12162289149470938No Hit
GGATCG324690.12151062075576845No Hit
ACGAAC320820.12006232822343045No Hit
GAGCCA309610.11586714494500436No Hit
CAGGAC308020.11527211002861744No Hit
CTCGTC303230.11347952056352725No Hit
ACACGA291820.10920949012580722No Hit
ACGCTA290750.10880905782358458No Hit
GAAGAT288330.10790340719612775No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)