FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23230911
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAC4495991.935348123024534No Hit
TGCGAC4029581.7345768317049641No Hit
TACTCG3956041.7029207335002918No Hit
CCATGA3890421.6746738860133379No Hit
CTTCAC3544531.5257817482921785No Hit
AGAACG3482111.4989123758426866No Hit
CCGGAA3473321.4951286240991581No Hit
TACAGT3441811.4815647995896501No Hit
AATTGC3320601.4293886279362873No Hit
TCCTCC3296231.418898294604116No Hit
TTCACA3276491.4104009954667727No Hit
GGCCGT3271781.4083735243960085No Hit
GCTCAA3249381.3987311991337747No Hit
CGTACT3243691.3962818763327878No Hit
TCCAGA3198141.3766743800964156No Hit
CACCTA3161421.3608678540415398No Hit
TCTTCA3033771.3059195138752846No Hit
ATTCGA3002821.2925967474973323No Hit
GTCTCA2990901.2874656529827866No Hit
TGCGGA2928891.2607727695224695No Hit
AACATA2926971.2599462844999922No Hit
AGGTCA2889531.2438298265616876No Hit
GCGTAT2852091.227713368623383No Hit
CATGAG2833491.2197067949681353No Hit
GTACTT2831131.2186909071280072No Hit
GTTGGC2823341.2153376163336858No Hit
CTGAGG2798851.20479562768761No Hit
CCTTAT2796321.2037065614861164No Hit
AGCCTA2756001.1863503760140959No Hit
TAGAGC2715351.1688521384288375No Hit
GCACAT2680391.1538032236445657No Hit
AGATGC2643201.1377943809435627No Hit
CCAGCG2629571.1319271982058732No Hit
CCGACT2612931.124764328011071No Hit
GCGGCT2604481.121126932990273No Hit
CATAAC2512951.0817268423093698No Hit
ACAGCC2502731.0773275314084756No Hit
GAATAC2494751.0738924530338048No Hit
GATTCA2476141.0658815747690653No Hit
GTCGAG2427111.0447760744294532No Hit
AACTAG2409311.037113869533571No Hit
GGCGTT2392571.0299079532438482No Hit
CCTCGG2337091.0060259797818518No Hit
CCTGCT2328471.0023154063996886No Hit
GCTGTC2322790.9998703882081938No Hit
CTCCGC2296050.9883598624264025No Hit
GAGTGA2227020.9586451431026531No Hit
GACTAA2226200.9582921651243036No Hit
TTAGTA2220380.9557868823999196No Hit
TGACAC2177960.9375267289345648No Hit
CAAGGA2173120.9354432979404036No Hit
AGGCTT2163290.9312118668097003No Hit
TATGGT2146390.9239370767681044No Hit
AAGAGG2123710.9141742224400928No Hit
GCCTGG2089090.8992716643785515No Hit
CAGTAG2086050.8979630630929627No Hit
GAGTCG2070600.8913124414277168No Hit
TTAGCG2063470.8882432548598718No Hit
AGAGGT2042960.8794145007916391No Hit
CCACTT2042090.8790399997658291No Hit
CTTAGC2033970.8755446568582694No Hit
ATGGCC1994300.8584682710032336No Hit
AACTCT1988620.8560232528117386No Hit
ATGTGA1970270.8481242943937928No Hit
TCTCGC1968710.8474527753130302No Hit
TGTGAT1958780.843178298087406No Hit
CCGCTC1923590.8280303772848169No Hit
GTTACA1906850.8208244609950941No Hit
AGGTGT1904360.819752613231569No Hit
GCGCAG1893760.8151897271699762No Hit
TACGGC1842820.793262046417379No Hit
GGCCAG1827010.7864564588104186No Hit
TCATGT1815970.781704169931175No Hit
ACCTGC1801110.7753075202259609No Hit
GGTAAT1799590.7746532195831666No Hit
CCTGAC1791120.7710072153433845No Hit
GGCAGG1726020.7429842075500181No Hit
AAGCGC1716390.7388388686091562No Hit
AGACGG1701930.732614403283625No Hit
ATGCCG1680890.7235575049123127No Hit
GTGGTG1671690.7195972641796097No Hit
TCGTGG1660840.7149267628807153No Hit
CGCTTG1645550.7083450149673425No Hit
CAAGCT1567250.6746399226444456No Hit
ATTGCT1554580.6691859824179947No Hit
GCATAA1550380.6673780464313259No Hit
GGTGGT1525850.6568188393472818No Hit
AGTTCC1447380.6230405686630197No Hit
GACACG1447000.6228769935023211No Hit
ACGTAA1427780.6146035340585654No Hit
CAACGG1423520.6127697704149442No Hit
AGTAGG1387490.5972602624150212No Hit
AACAAT1332270.5734902087998185No Hit
ACCAGG1288170.5545068809397962No Hit
CGTCTC1143340.4921632216661671No Hit
CTACCG956340.41166702416448503No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)