FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27350354
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAC5306171.940073609284911No Hit
TGCGAC4736941.731948332368934No Hit
TACTCG4630271.6929470090222598No Hit
CCATGA4569461.670713293144213No Hit
CTTCAC4176911.527186814474138No Hit
CCGGAA4082911.4928179723011996No Hit
AGAACG4079991.491750344437955No Hit
TACAGT4052821.4818162865460534No Hit
AATTGC3884821.4203911218114398No Hit
TCCTCC3871131.41538570213753No Hit
GGCCGT3841891.4046947984658626No Hit
TTCACA3837871.4032249820239988No Hit
CGTACT3814791.3947863343926006No Hit
GCTCAA3788561.3851959649224284No Hit
TCCAGA3744331.369024327802119No Hit
CACCTA3710991.356834357610143No Hit
TCTTCA3559161.3013213649812359No Hit
ATTCGA3504411.2813033425454017No Hit
GTCTCA3483841.2737824161252174No Hit
TGCGGA3459831.2650037363318953No Hit
AACATA3423431.2516949506393957No Hit
AGGTCA3387501.2385580091577608No Hit
GCGTAT3338871.2207776177229737No Hit
GTACTT3320031.2138892242491632No Hit
GTTGGC3311101.210624184242734No Hit
CATGAG3308711.209750338149188No Hit
CCTTAT3293151.2040611978916251No Hit
CTGAGG3271641.1961965830497112No Hit
AGCCTA3236111.1832058919603015No Hit
TAGAGC3195641.1684090085269097No Hit
GCACAT3143861.1494768952533485No Hit
AGATGC3101441.1339670411578584No Hit
CCAGCG3081191.1265631150514541No Hit
GCGGCT3069821.1224059476524508No Hit
CCGACT3059171.118512030959453No Hit
CATAAC2955911.0807574922064993No Hit
ACAGCC2953671.0799384900100377No Hit
GAATAC2934251.0728380334675012No Hit
GATTCA2889051.0563117391460455No Hit
GTCGAG2842391.0392516308929676No Hit
AACTAG2797531.0228496494049035No Hit
GGCGTT2795601.0221439912624166No Hit
CCTGCT2729430.9979505201285512No Hit
CCTCGG2727630.9972923933635374No Hit
GCTGTC2709140.9905319689829243No Hit
CTCCGC2681720.980506504595882No Hit
GACTAA2603140.9517756150432276No Hit
GAGTGA2602910.951691521067698No Hit
TTAGTA2598100.9499328601011893No Hit
CAAGGA2557800.9351981330844932No Hit
TGACAC2552170.9331396588139226No Hit
AGGCTT2538100.9279953012673986No Hit
TATGGT2529190.9247375737805806No Hit
AAGAGG2482600.9077030593461423No Hit
GCCTGG2440560.8923321431232664No Hit
CAGTAG2433380.8897069485828227No Hit
TTAGCG2425270.8867417218804553No Hit
GAGTCG2414680.8828697427462914No Hit
CCACTT2409980.8811513006376445No Hit
AGAGGT2403360.8787308566463161No Hit
CTTAGC2387110.8727894344621645No Hit
ATGGCC2338220.8549139802724308No Hit
AACTCT2321770.8488994328921665No Hit
TCTCGC2315610.8466471768518974No Hit
ATGTGA2310310.8447093591549126No Hit
TGTGAT2297370.839978158966425No Hit
CCGCTC2256460.8250204001015855No Hit
AGGTGT2225820.8138176200571298No Hit
GTTACA2220980.812047990311204No Hit
GCGCAG2204280.805942036435799No Hit
TACGGC2184580.7987392046187043No Hit
TCATGT2129010.7784213688788086No Hit
GGCCAG2126940.7776645230990429No Hit
ACCTGC2107230.7704580350221426No Hit
CCTGAC2099110.7674891520599697No Hit
GGTAAT2095490.7661655860103309No Hit
GGCAGG2014840.7366778506779107No Hit
AGACGG2008330.7342976255444446No Hit
AAGCGC2002600.7322025886758174No Hit
ATGCCG1970860.7205976200527423No Hit
TCGTGG1955300.7149084797951792No Hit
GTGGTG1948610.7124624419852116No Hit
CGCTTG1928750.7052011100112269No Hit
CAAGCT1841540.6733148682463123No Hit
ATTGCT1825830.6675708840916648No Hit
GCATAA1811200.6622217759960255No Hit
GGTGGT1784760.6525546250699351No Hit
AGTTCC1692170.6187013155295906No Hit
GACACG1687710.6170706236562788No Hit
ACGTAA1674690.6123101733893462No Hit
CAACGG1674290.612163922997121No Hit
AGTAGG1622180.5931111531499739No Hit
AACAAT1562520.5712979071495747No Hit
ACCAGG1507250.551089759203848No Hit
CGTCTC1334680.48799368373806057No Hit
CTACCG1115210.4077497497838602No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)