FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21149373
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAA3692291.7458153487576202No Hit
GTTACA3290691.5559279227805005No Hit
GACCAT3049781.4420191085570244No Hit
AGCCTA2814881.3309519861416221No Hit
AGACGG2755091.3026816445102178No Hit
TATAAG2635531.2461504177925276No Hit
GGATTC2576341.2181637725146746No Hit
GAGTGA2496211.1802761244978752No Hit
AGGTGT2382661.1265865895882587No Hit
TATGGT2376051.1234612014266332No Hit
ACTAAT2279821.077961034589536No Hit
AGTCAA2211651.0457284005535294No Hit
TATTCT2115871.0004410059815958No Hit
ACGTAA2088230.9873720606279912No Hit
CTAGTT2051260.9698916369766611No Hit
CCTTAT1983640.9379190579314101No Hit
AGGCTT1917790.9067833831291358No Hit
ACAGCC1913830.9049109871956961No Hit
TTCTGT1908880.9025704922788964No Hit
AAGCAT1846610.8731275390528126No Hit
GGTGGT1840580.8702763906996203No Hit
AACAAT1833850.8670942632672846No Hit
GCTGTC1804850.8533822728456301No Hit
CTGTAT1779140.8412258840959493No Hit
GCACAT1773310.8384693011939408No Hit
GGCGTT1766190.8351027711317967No Hit
AATCGG1743740.8244877992364125No Hit
AACTAG1733210.8195089282315839No Hit
TACGGC1656040.7830208488923053No Hit
AGATGC1653040.7816023671245478No Hit
CTTAGC1644320.7774793134529331No Hit
ATCAGC1637560.7742830012029198No Hit
CCTGCT1574000.7442301008167004No Hit
CCGACT1556210.7358185039338991No Hit
TCTTCA1546230.7310996879198263No Hit
CACCTA1535860.726196469275945No Hit
GTCTCA1528940.7229245046649846No Hit
ATTGAA1501730.7100588750314253No Hit
TACTCG1492620.7057514187300021No Hit
ATACCT1482390.7009143959019495No Hit
CCTGAC1473400.6966636788712366No Hit
TGACAC1462500.6915098617817181No Hit
TTAGCG1461590.6910795889788317No Hit
GTCTGC1454490.6877225154618059No Hit
AGTAGG1378340.6517167199235646No Hit
TCCAGA1378280.6516883502882095No Hit
CCTCGG1368500.6470640997253204No Hit
AGAACG1366270.6460096949446209No Hit
CCATGA1365200.6455037697807874No Hit
ACGCTA1315400.621956972436015No Hit
ATCCAC1312480.6205763168487312No Hit
CAAGCT1310970.6198623476922933No Hit
TGCGAC1309540.6191862047163289No Hit
TTCACA1288370.6091764517085211No Hit
AAGTTG1286390.6082402537418012No Hit
TCAAGC1279940.6051905179411229No Hit
GGCCAG1272010.6014409978016843No Hit
TAGAGC1268160.5996206128663956No Hit
TGGCAT1257780.5947126659499551No Hit
ACCTGC1241270.5869062879547303No Hit
CATGAG1238030.5853743276455524No Hit
TTCTAC1208850.5715772283178324No Hit
GTACGC1196260.5656243331658106No Hit
CTATTA1189930.5626313366358426No Hit
TGTGTG1189720.5625320429120996No Hit
ACCGCT1187900.5616714973063268No Hit
TTGAAT1165310.5509903295951137No Hit
GAACCG1149490.5435102024064733No Hit
CTATGC1146590.5421390033643078No Hit
GGCCGT1129100.5338692546582823No Hit
CACATT1123220.5310890303934779No Hit
AGTTCC1121600.5303230502388889No Hit
CAGCCT1120390.5297509292592267No Hit
GGTACC1109630.524663307985537No Hit
CAGTAG1101510.5208239506674737No Hit
CTCCGC1093760.5171595394341004No Hit
ATGGCC1087620.514256380082757No Hit
GTACTT1081420.5113248510960585No Hit
GCGCAG1080970.5111120788308948No Hit
AATGTA1073050.5073672869640155No Hit
ACTAGC1071700.5067289701685246No Hit
CATTAA1062720.5024829814103708No Hit
TCCTCC1061700.5020006976093334No Hit
CCGTCG1055410.49902661416960215No Hit
GAATAC1047960.4955040511130046No Hit
GACTAA1044830.4940241018019778No Hit
CATAAC1030380.48719174795394643No Hit
ACCAGG1027970.48605223426718136No Hit
ATTGCT1014990.4799149364853511No Hit
GCGTAT1011670.4783451499956996No Hit
GAGATA1008470.4768321027767584No Hit
ATGCCG1006720.4760046550789No Hit
GATTCA992880.4694607258569793No Hit
CGTCAG991400.46876094151821907No Hit
CTTCAC964790.4561790082382111No Hit
GATCCT964020.4558149312511534No Hit
GGACTA959910.4538716112293258No Hit
CAGTCC945770.4471858338306294No Hit
AACCTT933740.4414977219419224No Hit
AGAGGT932180.44076011142268856No Hit
AACATA931710.44053788261240656No Hit
CGTGAA928550.4390437484837021No Hit
TCGTGG923200.43651412266453476No Hit
AGGTCA919320.43467955291156857No Hit
TAATGA895880.4235964820328243No Hit
TCATGT893250.42235294634975706No Hit
TGATAA883970.41796510941482756No Hit
CGCTTG882680.4173551622546919No Hit
CGTCTC870130.41142118019290685No Hit
ATTCGA869410.41108074456864513No Hit
GCTCAA867150.41001215497026794No Hit
GAGCCA866530.40971900207159806No Hit
AAGGCG866330.40962443662041426No Hit
CGACAA852090.40289137649612594No Hit
TGCATA851220.4024800167834763No Hit
TCGGAC846900.40043740303790565No Hit
CCGCTC846820.4003995768574321No Hit
TGTGAT846630.4003097396788075No Hit
TATTGG840460.3973923955097865No Hit
TACGAA835290.3949478785966847No Hit
CTGTTC834450.3945507037017125No Hit
AAGCGC823040.38915574471167536No Hit
GCGGCT806220.38120279026711573No Hit
CAGGAC805080.38066376719536793No Hit
ACACGA800240.3783752832767194No Hit
ATGTGA792720.37481962231220756No Hit
CTACCG789050.37308434628298437No Hit
GAGTCG787950.37256423630147334No Hit
GGTAAT785760.37152874461101043No Hit
TCTCGC785060.37119776553186706No Hit
CGAATT780540.36906058633511263No Hit
GACACG779880.368748520346206No Hit
TAAGTC770190.3641668242363497No Hit
TTCGTT768160.3632069849068339No Hit
CATACG762480.36052132609321325No Hit
CGAGCC754890.35693256722078714No Hit
GTAAGA750650.35492777965569No Hit
AGTTAG738990.34941461385167305No Hit
CGAGAG734180.34714031475070206No Hit
GGCAGG711540.3364355056766931No Hit
AGTGCA696970.3295464125579515No Hit
GAAGAT690730.32659597048101613No Hit
TGCGGA676520.3198770951744054No Hit
TTCCAT674860.31909220192957966No Hit
ATCCGG659850.31199506481823364No Hit
CAAGGA657720.31098794276312586No Hit
GCTTCT653080.30879402429566116No Hit
GGATCG648790.3067655953677681No Hit
TCCACG645500.3052099936957942No Hit
GACCGA628700.2972664957963529No Hit
CGTACT624730.295389371590354No Hit
CCAGCG613830.29023555450083555No Hit
GCCATA607200.28710070979409175No Hit
GACTTG601700.2845001598865366No Hit
TCTCTG600990.284164452534834No Hit
TTACGT599020.28323298284067333No Hit
GAGCAC588320.2781737312023387No Hit
ATTCAT586790.2774503055007824No Hit
GTGGTG584810.27651410753406264No Hit
CTCGTC575480.2721026292363372No Hit
GTTGGC569260.2691616437045202No Hit
TTAGTA563050.2662253864452625No Hit
CTGGAG540610.25561514282243736No Hit
ACGAAC536140.2535016049884789No Hit
CCGGAA532230.2516528504178351No Hit
GAGAAG515110.24355804779649967No Hit
GTCGAG510770.24150597750581068No Hit
CCACTT503070.23786520763523344No Hit
GGAGGC486160.22986969873764104No Hit
CGAAGC486100.2298413291022859No Hit
TCAATG479710.2268199629369627No Hit
CAACGG465450.22007744626755602No Hit
AATTGC454290.21480069409149863No Hit
TACAGT433520.2049800719860584No Hit
AGGACT427470.2021194670877477No Hit
CTGAGG422570.199802613533744No Hit
AAGAGG385130.182099961072132No Hit
CGTTGC372850.17629364236944517No Hit
AACTCT314340.14862851962561727No Hit
GCCTGG255680.12089247279340148No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)