FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23001967
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCC3033591.3188393844752495No Hit
GGTACC2793851.2146135154441358No Hit
GCCAAT2358191.0252123220592395No Hit
AAGCGC2006760.8724297361177851No Hit
CGTACT1905480.8283987191182389No Hit
CGCTTG1896710.8245860017102015No Hit
ACAGTG1882760.8185213029824797No Hit
GCACAT1882330.8183343624482201No Hit
TGACCA1796390.7809723403220256No Hit
GATCAG1738560.7558310121912617No Hit
GTCTCA1712970.7447058766756773No Hit
CATACG1706530.7419061161160695No Hit
AACTAG1702330.7400801853163252No Hit
TAGAGC1645850.7155257635140508No Hit
TTAGGC1639920.7129477231229834No Hit
AGTTCC1615350.7022660279444797No Hit
CGATGT1602000.6964621764738642No Hit
ACTAGC1581500.6875498951893984No Hit
CAAGGA1570230.6826503142100847No Hit
GCTGTC1569100.6821590518758678No Hit
GCCATA1559910.6781637413878561No Hit
ACTTGA1558080.6773681572536818No Hit
CCTGCT1545450.6718773224915939No Hit
GCATAA1541300.6700731289632752No Hit
CCTTAT1530630.665434395241068No Hit
TCTTCA1526490.6636345491670342No Hit
TGTGAT1520280.6609347800559838No Hit
CTATTA1512900.6577263587935762No Hit
GGACTA1510860.6568394781194148No Hit
TATAAG1500500.6523355154800456No Hit
CGAATT1500020.6521268376743606No Hit
CAGGAC1481330.6440014456154989No Hit
AGGTGT1478810.6429058871356524No Hit
GACTAA1478220.6426493873328312No Hit
ATTCAT1410280.613112782919826No Hit
AGTTAG1404720.6106955983373074No Hit
ACTAAT1400730.6089609640775504No Hit
ATGTGA1390870.6046743741524366No Hit
CTTAGC1376040.5982270994476255No Hit
ATTGCT1365210.5935188064568565No Hit
TCTCTG1361170.5917624349256739No Hit
GAACCG1355640.5893582927060107No Hit
CTTCAC1354580.5888974625517897No Hit
GTAAGA1353750.5885366238461259No Hit
TACGGC1339530.5823545438527061No Hit
GAGCAC1335220.5804807910558257No Hit
TATTCT1334930.5803547148815578No Hit
GAAGAT1312780.5707251036400496No Hit
TGCGAC1292000.5616910936356009No Hit
CATAAC1290830.5611824414842436No Hit
GTCTGC1287440.5597086544815928No Hit
TGATAA1276520.5549612344022579No Hit
TACTCG1271500.552778812351135No Hit
AAGCAT1270650.552409278736901No Hit
CTGGAG1265760.5502833735914846No Hit
GAATAC1263840.5494486623687443No Hit
CGTCAG1263750.5494095352801784No Hit
CAGATC1259880.5477270704718427No Hit
TTAGTA1259240.547448833397596No Hit
CGTCTC1255120.5456576822321326No Hit
GTTACA1254860.5455446484207199No Hit
AGTAGG1244520.541049380689921No Hit
TAAGTC1242360.5401103305643383No Hit
GACCGA1231240.5352759613993012No Hit
ATCAGC1228210.5339586827509143No Hit
CGAAGC1222610.5315241083512554No Hit
ACAGCC1221310.5309589392941917No Hit
CATGAG1210440.5262332564862822No Hit
AATGTA1198870.5212032518784154No Hit
ACCTGC1198410.5210032689813006No Hit
TTCCAT1186500.5158254509277402No Hit
ATTCGA1186430.5157950187477445No Hit
TAGCTT1186130.5156645951191913No Hit
GCGTAT1184090.5147777144450298No Hit
TCAAGC1181690.5137343254166046No Hit
TTGAAT1178270.5122474960510986No Hit
CCGCTC1174990.5108215310455841No Hit
AACATA1174560.5106345905113245No Hit
AGTGCA1170210.5087434478973037No Hit
CGTGAA1162730.505491552092045No Hit
CTGTAT1162670.5054654673663344No Hit
TGGCAT1158170.503509112938037No Hit
GGTAAT1155860.5025048509981778No Hit
AGTCAA1153140.501322343432629No Hit
AAGTTG1152550.5010658436298079No Hit
ACGCTA1149620.499792039524272No Hit
TTCTGT1149590.49977899716141666No Hit
TATGGT1133660.492853502485244No Hit
ATTGAA1123620.4884886583829983No Hit
TTCTAC1122360.487940879143075No Hit
TCGGAC1119880.48686271048036894No Hit
AGAGGT1119350.4866322954032583No Hit
TGTGTG1118960.48646274468613926No Hit
ATACCT1117640.48588888072050535No Hit
CTACCG1117200.48569759273196067No Hit
CTCCGC1117170.48568455036910535No Hit
AATCGG1116220.4852715422120204No Hit
ATCCAC1113390.48404121264933564No Hit
GCGCAG1113210.4839629584722037No Hit
TCCAGA1110060.48259351037239556No Hit
AGATGC1109790.48247612910669774No Hit
CACCTA1101840.47901990295003904No Hit
CCTGAC1099700.4780895477330265No Hit
GATCCT1093470.47538108371340587No Hit
GCTCAA1084450.47145967994824095No Hit
GAGATA1084090.4713031715939771No Hit
CAAGCT1083270.4709466803425985No Hit
CACATT1073440.46667313278034No Hit
GGCAGG1071370.46577320974332326No Hit
GAGTGA1065100.4630473559065622No Hit
GAGAAG1064790.46291258482372394No Hit
TAATGA1061200.46135184873537116No Hit
TCTCGC1055680.4589520539699931No Hit
AACCTT1046830.45510455692767493No Hit
TGCATA1045370.45446982860204954No Hit
AAGAGG1045310.45444374387633896No Hit
GCTTCT1044380.4540394306278241No Hit
CGTTGC1039100.45174397476528855No Hit
CGACAA1027040.4465009448974516No Hit
TTACGT1026690.4463487839974729No Hit
TTCGTT1026250.4461574960089283No Hit
AAGGCG1024080.4452140984290604No Hit
ATCCGG1021720.44418809921777563No Hit
ACACGA1020320.44357945561786083No Hit
GGATTC1018780.44290994765795466No Hit
CTGTTC1014340.4409796779553679No Hit
CGAGAG1013820.44075361033254246No Hit
TTCACA1010900.4394841536812917No Hit
TGCGGA1007500.43800601922435584No Hit
ACCGCT1000660.4350323604933439No Hit
TACAGT992630.4315413547024044No Hit
CTCGTC990430.4305849147596812No Hit
AATTGC984580.4280416540028946No Hit
CATTAA983980.42778080674578833No Hit
TTAGCG982280.42704173951732044No Hit
CTAGTT977810.42509842745187837No Hit
TACGAA975980.42430284331770407No Hit
GGCGTT974940.42385070807205316No Hit
CAGCCT970580.42195521800374725No Hit
ACGAAC970550.4219421756408919No Hit
TCGTGG970110.42175088765234725No Hit
CGAGCC970000.42170306565521115No Hit
GAGCCA968930.4212378880467048No Hit
GACTTG966610.4202292786525605No Hit
TCCTCC964690.41939456742982023No Hit
GGATCG954270.4148645200647405No Hit
AGGTCA950380.4131733603478346No Hit
TCATGT945950.41124743809953296No Hit
ATGCCG945630.41110831956240956No Hit
CCGGAA940570.4089085076941464No Hit
AACTCT940050.4086824400713208No Hit
CCATGA938250.40789989830000195No Hit
CTATGC935440.40667826364588733No Hit
ACCAGG934760.4063826367545002No Hit
GACACG930480.4045219263204751No Hit
TGACAC926650.4028568513292798No Hit
CAGTAG925930.4025438346207522No Hit
AGCCTA923920.40166999630944605No Hit
AGGACT922340.4009830985323994No Hit
TATTGG919070.39956148098117No Hit
CAGTCC918660.3993832353554807No Hit
GATTCA914020.39736601656719184No Hit
GTACGC911720.39636610208161766No Hit
GACCAT910530.3958487550216901No Hit
AGGCTT886010.38518879711461196No Hit
TCCACG878470.3819108165836426No Hit
CCAGCG877300.38140216443228525No Hit
GGAGGC877130.3813282577094385No Hit
TCAATG873300.3796631827182432No Hit
AGAACG869240.37789811627849046No Hit
CCGACT865910.3764504140015504No Hit
GGCCAG847740.36855108956551413No Hit
CCTCGG845960.3677772427027654No Hit
CCACTT845900.36775115797705477No Hit
GAGTCG843710.3667990654886167No Hit
GTGGTG836080.3634819578690814No Hit
CTGAGG830370.36099956147228623No Hit
GCCTGG824060.35825631781838485No Hit
ACGTAA823220.357891131658436No Hit
AACAAT821120.3569781662585639No Hit
GTCGAG814230.35398277025612634No Hit
CAACGG812620.3532828301162244No Hit
GGTGGT809100.35175252620786734No Hit
CCGTCG782510.34019264526377246No Hit
GGCCGT732150.3182988654839823No Hit
GTACTT698040.3034696989174882No Hit
GCGGCT671040.291731572347704No Hit
GTTGGC644990.2804064539350048No Hit
AGACGG415190.18050195446328568No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)