FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25613582
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA2812011.0978589406198633No Hit
ACCAGG2697481.0531443825389202No Hit
GCATAA2679821.0462496030426358No Hit
AATCGG2306380.9004519555289066No Hit
TGACCA2182340.8520245235516063No Hit
GACTAA2175870.8494985199649154No Hit
TATAAG2154500.8411552901894003No Hit
AGACGG2137770.8346235993075861No Hit
GATCAG2077240.8109916059378185No Hit
GCCAAT2000660.7810934058344514No Hit
GGACTA1993600.7783370557073978No Hit
TATTCT1978670.7725081169826228No Hit
ACCGCT1945210.7594447352189944No Hit
TCTCTG1939850.7573520954624777No Hit
TTAGCG1928960.7531004449123907No Hit
CGTACT1921410.7501527900314763No Hit
GGTAAT1886270.7364335062546112No Hit
TATTGG1874970.7320217843798654No Hit
GAACCG1854520.7240377390401701No Hit
TGGCAT1852980.7234364955280366No Hit
CGATGT1846520.720914396119996No Hit
GAGCCA1789620.6986996196002574No Hit
CGAGAG1789040.6984731772385447No Hit
AACTCT1781340.6954669596778772No Hit
ACAGTG1773970.6925895800126667No Hit
GGCGTT1768090.6902939229663387No Hit
ACTTGA1751710.6838988783372821No Hit
CTTAGC1747840.682387961199648No Hit
CAGATC1746960.6820443934784287No Hit
GATCCT1736930.6781285022922604No Hit
GGCTAC1723700.6729632739380224No Hit
TACGGC1719740.6714172191925362No Hit
AGTCAA1717480.670534874817587No Hit
TACTCG1699670.6635815326415493No Hit
TAGCTT1671980.6527708619590965No Hit
CCTGAC1661080.6485153072303592No Hit
AACATA1651260.6446814037958455No Hit
CGTCTC1639640.6401447482042926No Hit
AGCCTA1624000.6340386127953521No Hit
GTTACA1613500.6299392252126235No Hit
AGTAGG1608200.6278700105280082No Hit
GCTGTC1607050.6274210299832331No Hit
CTTCAC1598140.6239424068058891No Hit
ATCCAC1595570.6229390328927833No Hit
AGTTCC1594390.6224783398120575No Hit
GGATCG1591650.6214085948618979No Hit
TATGGT1587060.6196165768614479No Hit
GGATTC1570760.6132527656615931No Hit
TCCTCC1567580.6120112368508239No Hit
TGTGTG1563780.6105276489637412No Hit
GAATAC1543450.6025904537678486No Hit
CATAAC1539160.6009155611269053No Hit
CGAATT1518460.5928339113209546No Hit
CCTTAT1508700.5890234329583421No Hit
GTCTCA1503290.5869112723085744No Hit
AGTGCA1487250.5806489697536252No Hit
TCAATG1483590.5792200403676456No Hit
GGTGGT1467020.5727508163442349No Hit
ATCAGC1456420.5686123869750042No Hit
CAAGCT1450220.5661917962118692No Hit
TAGAGC1446370.5646886874315354No Hit
GAAGAT1442410.5631426326860491No Hit
ACGTAA1430350.5584341932338867No Hit
CCGACT1427890.5574737652859331No Hit
AACTAG1425310.5564664871941769No Hit
GTAAGA1423180.5556348971416806No Hit
GACACG1421120.5548306363397357No Hit
ACGCTA1421000.5547837861959332No Hit
TTAGGC1415770.5527419007618692No Hit
TCTCGC1405240.54863080064319No Hit
CTCCGC1403780.5480607905602581No Hit
ATTGCT1402980.5477484562682408No Hit
GGAGGC1402590.5475961933008824No Hit
ATGGCC1363870.5324792135672395No Hit
CTGTTC1360230.5310580925385602No Hit
ACTAAT1351720.527735636507225No Hit
CATGAG1333820.5207471567233353No Hit
TTCTAC1331860.5199819377078927No Hit
GCGTAT1328500.5186701336814195No Hit
AAGCAT1320720.5156326826915502No Hit
GGTACC1313190.5126928361679362No Hit
TTCCAT1312620.5124702979848738No Hit
TAAGTC1311580.5120642634052511No Hit
GCGCAG1309630.5113029485684587No Hit
GTACTT1302760.5086207778357592No Hit
AGGTGT1283280.5010154378251351No Hit
GATTCA1277990.49895012731917No Hit
GTACGC1273720.49728304303552695No Hit
TGCGGA1272550.49682625413345155No Hit
TTCACA1262380.49285570444618015No Hit
TCCAGA1256990.4907513521537128No Hit
CACCTA1256020.4903726468246417No Hit
CAACGG1244820.48599996673639784No Hit
TACAGT1243390.48544166918941684No Hit
GGCAGG1243000.4852894062220583No Hit
TCGGAC1239420.4838917102652804No Hit
AGGCTT1238010.4833412210755996No Hit
ACAGCC1237050.4829664199251788No Hit
AATTGC1235110.4822090092670366No Hit
TTACGT1229280.4799328731139596No Hit
GGCCGT1222810.4774068695272688No Hit
TAATGA1219190.47599355685588995No Hit
TACGAA1217060.47516196680339357No Hit
ATGCCG1204930.47042619810067954No Hit
AGAGGT1203110.46971563758633994No Hit
CAGTAG1201840.46921980689776227No Hit
TCTTCA1191990.4653741909272978No Hit
GAGATA1190100.4646363011624067No Hit
GTGGTG1184770.4625553739418407No Hit
CCGCTC1182710.4617511131398959No Hit
CCTGCT1178600.4601464957146564No Hit
TGCGAC1178420.46007622049895247No Hit
TCAAGC1174720.45863167439837194No Hit
GACCGA1170030.45680061461141985No Hit
GCCATA1168240.4561017666330308No Hit
ATTCGA1159240.45258800584783493No Hit
TTCTGT1154590.4507725627754837No Hit
GCGGCT1151650.44962473425231975No Hit
GCACAT1149640.4488399943436259No Hit
CGACAA1139110.44472889422494677No Hit
ATTCAT1131880.44190617306083935No Hit
CAAGGA1127270.440106346703089No Hit
TGACAC1126120.43965736615831397No Hit
AAGCGC1125250.43931770261574504No Hit
ATACCT1123560.4386578964238583No Hit
TGTGAT1122930.4384119331688945No Hit
CGAGCC1114470.4351089980308104No Hit
GAGTGA1103370.4307753597290688No Hit
TTGAAT1099570.429291771841986No Hit
GACCAT1088330.42490347503914133No Hit
GCTTCT1082990.4228186436399251No Hit
ATGTGA1081950.4224126090603025No Hit
CATTAA1077190.42055422002279885No Hit
CCTCGG1073530.4191252906368192No Hit
CCAGCG1070650.4180008871855565No Hit
ATCCGG1065510.41599413935934454No Hit
GGCCAG1062150.41468233533287147No Hit
AGTTAG1057360.41281223375941717No Hit
CCATGA1051480.41051657671308917No Hit
ACTAGC1045760.40828338652516466No Hit
AGGTCA1045330.4081155068432053No Hit
GAGTCG1044040.4076118677973272No Hit
TCGTGG1041770.4067256192437278No Hit
GTTGGC1029770.40204060486346654No Hit
GAGCAC1029270.4018453959309557No Hit
CAGGAC1025700.40045160415282793No Hit
GAGAAG1025610.40041646654497604No Hit
CGCTTG1016810.39698078933278447No Hit
CTAGTT1015580.3965005753588077No Hit
AGATGC1009640.3941814932405784No Hit
CCACTT1006850.3930922273971677No Hit
AAGGCG1006320.3928853059287061No Hit
TGATAA1004590.39220988302221843No Hit
CTACCG996660.38911386935259584No Hit
ACCTGC993940.3880519327597366No Hit
AATGTA986830.3852760617394318No Hit
AAGTTG982470.3835738398479369No Hit
CTGGAG981140.383054584087458No Hit
CTATGC972670.3797477447707236No Hit
CCGTCG971260.3791972555810429No Hit
CACATT968640.37817436077468586No Hit
TTAGTA938670.3664735373599835No Hit
ATTGAA937490.3660128442792578No Hit
CGTGAA931660.36373670812618086No Hit
AGGACT919650.35904778956726946No Hit
CAGCCT919460.3589736101729153No Hit
ACACGA914540.35705275427700817No Hit
GCTCAA903520.3527503494044683No Hit
CTCGTC900170.3514424495566454No Hit
AGAACG892060.34827616067131884No Hit
CTGTAT891670.34812389770396035No Hit
TCCACG889450.34725717004361206No Hit
CAGTCC886940.3462772212024074No Hit
TTCGTT871570.3402764986170228No Hit
GTCTGC869820.3395932673532347No Hit
GTCGAG867730.3387772940153392No Hit
GACTTG847660.3309416074643523No Hit
CTGAGG836850.32672119034346697No Hit
AAGAGG820520.3203456666076615No Hit
TGCATA818590.3195921601281695No Hit
CCGGAA806590.3149071457479083No Hit
AACCTT796810.3110888590279954No Hit
TCATGT779830.30445956367992577No Hit
GCCTGG759070.2963544888020738No Hit
CGAAGC754190.29444924962076763No Hit
CGTTGC740450.2890849081553685No Hit
ACGAAC736230.2874373447649767No Hit
CATACG602640.23528142217671857No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)