FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24585741
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA2766491.1252416593829733No Hit
GCATAA2671991.0868047458890908No Hit
ACCAGG2651201.0783486249204366No Hit
AATCGG2270190.9233766840706571No Hit
AGACGG2164180.8802581951872023No Hit
GACTAA2155290.87664227814No Hit
TGACCA2149520.8742953893478338No Hit
GATCAG2019710.8214964926214753No Hit
TATAAG1973550.802721382284146No Hit
GCCAAT1971120.801733004508589No Hit
GGACTA1957350.796132197113766No Hit
TATTCT1941160.7895470793416395No Hit
ACCGCT1926370.7835313973249779No Hit
TTAGCG1884130.7663507071029504No Hit
CGATGT1834050.7459811766503194No Hit
TATTGG1830740.7446348678284702No Hit
GGTAAT1829850.7442728693839247No Hit
TCTCTG1780820.7243304157478923No Hit
TACGGC1778190.7232606900072688No Hit
GAGCCA1777910.7231468028561758No Hit
CGTACT1774390.7217150786710069No Hit
TGGCAT1763950.7174687148945399No Hit
GGCGTT1756350.714377492222016No Hit
CGAGAG1755940.7142107288936298No Hit
AACTCT1747530.710790046962587No Hit
ACAGTG1730110.7037046392053019No Hit
CTTAGC1723710.7011015043231766No Hit
GATCCT1717880.6987302111414905No Hit
CAGATC1709890.6954803599370871No Hit
ACTTGA1705240.6935890197492929No Hit
GAACCG1704680.6933612454471069No Hit
GGCTAC1699670.6913234789221931No Hit
AGTCAA1680560.6835506808600968No Hit
CCTGAC1652420.6721050221752519No Hit
TACTCG1649480.6709092070887755No Hit
TAGCTT1629930.6629574435035333No Hit
CGTCTC1624600.6607895202345132No Hit
AGCCTA1609780.6547616360230917No Hit
AACATA1606120.6532729682623761No Hit
CTTCAC1586300.6452113849242941No Hit
GTTACA1583670.6441416591836707No Hit
ATCCAC1578280.6419493315251308No Hit
AGTAGG1575140.640672168473588No Hit
GCTGTC1573560.6400295195495633No Hit
AGTTCC1573420.6399725759740168No Hit
TATGGT1561230.6350144175032186No Hit
GGATCG1559400.634270083622861No Hit
TCCTCC1559200.6341887356577945No Hit
GGATTC1546270.6289295897162506No Hit
GAATAC1516050.6166379121947149No Hit
CGAATT1485510.6042160779290728No Hit
CCTTAT1479710.6018569869421466No Hit
GTCTCA1471740.5986152705342499No Hit
TGTGTG1451450.5903625194782618No Hit
GGTGGT1447250.588654212211867No Hit
TTAGGC1438660.5851603171122645No Hit
CATAAC1420940.5779528874073797No Hit
CCGACT1410030.5735153559130066No Hit
TAGAGC1409770.5734096035584203No Hit
ACGTAA1408230.5727832242274089No Hit
GAAGAT1402220.5703387178771631No Hit
TCTCGC1391780.5660923541006959No Hit
CTCCGC1389010.5649656847845261No Hit
GACACG1387080.5641806769216352No Hit
AACTAG1381170.5617768445539226No Hit
GTAAGA1379020.5609023539294586No Hit
AGTGCA1370730.5575304807774555No Hit
ATCAGC1365370.5553503553136756No Hit
GGAGGC1357550.5521696498795785No Hit
ATGGCC1357100.5519866169581791No Hit
TCAATG1344330.5467925493886884No Hit
CAAGCT1336420.5435752373703114No Hit
ACGCTA1305980.5311940770872027No Hit
GGTACC1303030.5299941946024731No Hit
GCGTAT1302290.5296932071317273No Hit
ATTGCT1292390.5256664828609395No Hit
CATGAG1289960.5246781050853826No Hit
TGCGGA1274510.5183939747840018No Hit
GTACTT1273650.5180441785342162No Hit
AGGTGT1268380.5159006596547161No Hit
CTGTTC1258520.5118902049769417No Hit
ACTAAT1237930.503515431973354No Hit
GGCAGG1237360.5032835902729147No Hit
TCCAGA1226580.49889893495583476No Hit
TTCTAC1226370.4988135195925151No Hit
AAGCAT1219280.4959297342309105No Hit
ACAGCC1218850.4957548361060177No Hit
TAAGTC1217700.49528708530688587No Hit
TTCCAT1216660.4948640758885404No Hit
CAACGG1215120.49423769655752897No Hit
TACAGT1212950.49335507113655835No Hit
GGCCGT1211150.49262293945096064No Hit
GCGCAG1200460.4882748907181606No Hit
GTACGC1193540.48546025112686253No Hit
AGAGGT1192760.48514299406310346No Hit
CCGCTC1188350.48334927143338896No Hit
ATGCCG1178880.4794974452874941No Hit
GATTCA1172550.476922782193142No Hit
CACCTA1171990.47669500789095604No Hit
CAGTAG1171800.47661772732414287No Hit
TTCACA1167340.4748036677031618No Hit
GCGGCT1161720.4725177898847954No Hit
GAGATA1161470.4724161049284624No Hit
AGGCTT1159990.4718141299869709No Hit
TCGGAC1153820.46930454526467197No Hit
AATTGC1141990.4644928131309933No Hit
TACGAA1130990.4600186750523403No Hit
AAGCGC1127480.45859101826542464No Hit
TAATGA1124780.45749282073702807No Hit
TTACGT1122090.45639869060688465No Hit
CAAGGA1120450.4557316372933401No Hit
TGACAC1112000.45229468576928394No Hit
CGAGCC1099870.44736093168800567No Hit
TGCGAC1099700.44729178591769925No Hit
TCTTCA1099030.44701927023472665No Hit
CCTGCT1097580.44642949748799515No Hit
GAGTGA1093600.44481067298317345No Hit
GACCGA1091760.4440622717045624No Hit
GTGGTG1082300.4402145129569208No Hit
GCCATA1079960.4392627417656438No Hit
TCAAGC1077450.4382418248040602No Hit
ATTCGA1066860.43393445005379333No Hit
CGACAA1066150.4336456647778076No Hit
TTCTGT1065780.4334951710424347No Hit
CCTCGG1059160.4308025533987363No Hit
GCACAT1052200.42797164421442496No Hit
GGCCAG1046720.42574270997160507No Hit
ATACCT1043390.4243882663532492No Hit
ATTCAT1038860.4225457349444949No Hit
TGTGAT1023870.4164487049627668No Hit
GAGTCG1022610.41593621278284837No Hit
CAGGAC1019760.4147770042806519No Hit
GTTGGC1016200.4133290105024697No Hit
GACCAT1016070.4132761343251765No Hit
GCTTCT1012480.4118159383522343No Hit
TTGAAT997830.4058571999111192No Hit
CATTAA995010.4047101936036827No Hit
ATGTGA987250.4015538925591057No Hit
CCAGCG983430.40000014642633713No Hit
ATCCGG979380.3983528501337421No Hit
CCATGA976620.3972302482158256No Hit
AGTTAG971210.39502978576077896No Hit
AGGTCA969320.3942610474909013No Hit
ACTAGC965840.39284559289874565No Hit
TCGTGG963370.3918409455301754No Hit
GAGCAC952190.38729359428296267No Hit
CTGGAG952030.3872285159109095No Hit
CACATT942540.38336855496850797No Hit
CGCTTG941850.38308790448902885No Hit
AGATGC941020.38275031043400315No Hit
CTAGTT934290.38001295140951824No Hit
ACCTGC933860.37983805328462544No Hit
CCACTT933260.37959400938942617No Hit
AAGGCG928870.3778084215562183No Hit
GAGAAG928870.3778084215562183No Hit
TGATAA924300.37594962055445064No Hit
CTACCG922860.3753639152059724No Hit
CTATGC910110.37017798243298833No Hit
AAGTTG904110.36773754348099574No Hit
CCGTCG902590.367119298946491No Hit
AATGTA898630.3655086092381759No Hit
ACACGA897260.36495137567747093No Hit
TCCACG865100.35187062289479093No Hit
ATTGAA859860.3497393062100508No Hit
CGTGAA858660.3492512184196523No Hit
TTAGTA853400.3471117669384055No Hit
CAGCCT850740.34602983900302214No Hit
GTCGAG850350.3458712104711426No Hit
AGGACT845240.3437927699636956No Hit
CTCGTC842020.3424830677261263No Hit
GCTCAA836710.3403232792536129No Hit
CAGTCC825820.3358938825557464No Hit
AGAACG814420.33125704854696064No Hit
CTGTAT813940.3310618134308012No Hit
GTCTGC811450.3300490312657243No Hit
TTCGTT801960.3261890703233228No Hit
GACTTG777150.3160978552568336No Hit
CTGAGG765580.31139187547774133No Hit
CCGGAA755600.3073326120209271No Hit
TGCATA754430.30685672642528855No Hit
AAGAGG748170.30431053511870965No Hit
AACCTT734280.2986609189448469No Hit
TCATGT714680.29068881836833793No Hit
CGAAGC710030.28879747818054374No Hit
GCCTGG709110.28842327754123825No Hit
CGTTGC695270.2827939983586421No Hit
ACGAAC675470.2747405498170667No Hit
CATACG637460.259280369056194No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)