FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23891540
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGG11585034.849009314594204No Hit
GGCGTT9519733.98456106220026No Hit
AGCCTA8814283.689289179349678No Hit
GAATAC8167693.4186536322062118No Hit
CCTTAT7997113.3472559742904813No Hit
GGTGGT7862283.2908217720582265No Hit
CCGACT7725083.2333955868897526No Hit
ATGGCC7285433.0493764738480653No Hit
ATGCCG6362502.663076553457835No Hit
CAGTAG6329142.6491134518745967No Hit
GGCCAG5621252.3528202870137296No Hit
GGTAAT3840631.6075271832623597No Hit
CTTAGC3590011.5026281269436796No Hit
TACTCG3519891.4732788258940195No Hit
CGTCTC3436011.4381701639994744No Hit
TTAGCG3106791.300372433087193No Hit
CTCCGC2956751.2375719606186961No Hit
TCTCGC2912061.2188665946188484No Hit
AACTCT2878641.2048783795435538No Hit
GCATAA2855931.1953729228002883No Hit
CCTGAC2731551.143312653767819No Hit
AGGTGT2687661.1249421343287205No Hit
CTTCAC2638161.1042235033823689No Hit
AGTTCC2610401.0926043277243744No Hit
TCCAGA2575641.0780552446598253No Hit
AGAGGT2525251.05696409691464No Hit
GGCCGT2497861.0454997877909922No Hit
GTCTCA2396751.0031793680943129No Hit
AAGCGC2348940.9831681005075437No Hit
TAGAGC2309750.9667648046128463No Hit
GTACTT2086550.8733426141638421No Hit
GGCAGG2065520.8645403351981498No Hit
TACAGT1989750.8328261803131988No Hit
TGACAC1853060.7756134598272024No Hit
TACGGC1844080.7718548071827935No Hit
CTATTA1842660.771260454537464No Hit
CAAGGA1837480.7690923230566133No Hit
GTCGAG1790670.7494996136707806No Hit
GTTACA1708930.7152866663262394No Hit
AGTAGG1699580.711373147147484No Hit
TATGGT1689750.7072587200322792No Hit
ACGTAA1519420.6359657016667825No Hit
AACTAG1458950.6106554872561585No Hit
CAACGG1288240.5392034167743058No Hit
ACAGCC1287320.5388183432294443No Hit
ACCAGG1236590.5175848856959409No Hit
TATTGG1230380.5149856392681259No Hit
GAGCCA1200370.5024247076580246No Hit
CGAGAG1188830.49759454601921854No Hit
GAGTGA1183090.4951920219458436No Hit
GATCCT1153300.4827231731399483No Hit
CCTCGG1120730.4690907325354498No Hit
GAGTCG1089790.45614054179847763No Hit
GGATCG1054090.44119801402504816No Hit
AATCGG1041260.43582791230703416No Hit
GACTAA1014920.42480308929436944No Hit
GAAGAT950130.3976847034557003No Hit
CGTACT912720.38202644115866957No Hit
TATTCT891260.3730441821665744No Hit
ACCGCT890690.37280560399204066No Hit
GGACTA888100.37172153825161547No Hit
GGTACC863030.36122828415413993No Hit
TGACCA855060.3578923752926768No Hit
GCCAAT791720.33138089884536537No Hit
GATCAG783910.3281119592960521No Hit
AGTCAA774320.324097986149072No Hit
TCCTCC760350.3182507280819905No Hit
AACATA759090.31772334474881064No Hit
ATCCAC735190.30771980374643076No Hit
CGAGCC733810.3071421934291385No Hit
GCTGTC729460.3053214652550652No Hit
CGATGT724720.30333749938262666No Hit
GGATTC711450.2977832320562006No Hit
ACAGTG695320.2910318882750966No Hit
CAGGAC681250.2851427743879214No Hit
CGAATT677010.28336808761595106No Hit
ACTTGA671810.2811915849710818No Hit
CAGATC664260.278031470554012No Hit
GGCTAC661150.2767297545490998No Hit
GACACG650350.2722093259789867No Hit
GTAAGA631420.2642860192352607No Hit
TAGCTT629910.2636539963518467No Hit
GCGCAG623270.26087476989762903No Hit
GCGTAT622790.2606738619611796No Hit
TTCACA621880.2602929739983274No Hit
CATAAC610160.25538747188335287No Hit
CATGAG604950.2532067836564742No Hit
ACACGA604050.2528300812756315No Hit
TGCGGA602110.2520180783658148No Hit
TATAAG600090.2511725907999233No Hit
TGCGAC589520.24674843061602558No Hit
TCCACG584510.24465145402933425No Hit
CAAGCT570220.2386702573379531No Hit
TCTTCA567070.23735179900500344No Hit
CCGCTC566410.23707555059238541No Hit
TTAGGC566010.23690812731201083No Hit
GCGGCT552180.2311194673930605No Hit
ATGTGA531370.22240927123157403No Hit
GAGATA525980.22015324252852683No Hit
AGGTCA510990.21387905509649024No Hit
CCAGCG510340.21360699226588156No Hit
CACCTA499740.20917027533595575No Hit
AGGCTT492820.20627385258547587No Hit
CTACCG491830.20585947996654883No Hit
GTTGGC469540.19652981766767652No Hit
CCTGCT457240.19138155179615882No Hit
GCACAT440710.18446278473468014No Hit
AGAACG436560.1827257682007941No Hit
GCTCAA436370.18264624214261618No Hit
TCTCTG436150.18255415933841015No Hit
CTGGAG433270.18134871171971334No Hit
CACATT429600.17981260312227676No Hit
ATCAGC418620.1752168340759951No Hit
CCATGA410600.17185999730448517No Hit
TCGTGG404470.16929423553274506No Hit
AGATGC402360.16841107772876926No Hit
ACCTGC400960.1678250962474583No Hit
CCGGAA391470.16385297892057188No Hit
ACTAAT379360.15878423910723208No Hit
TTAGTA359340.1504047039244854No Hit
ACGCTA336850.1409913299854258No Hit
TTCTGT322660.1350519891141383No Hit
ATACCT322530.13499757654801658No Hit
GGAGGC322420.13495153514591357No Hit
AAGAGG318690.13339031305642082No Hit
ATTCAT318640.133369385146374No Hit
GACCAT313520.13122636715757963No Hit
ATTGCT295330.12361279348254653No Hit
GTACGC290240.12148233223978028No Hit
CTAGTT286850.12006341993860588No Hit
TGTGTG283910.11883285882785287No Hit
TGATAA278150.11642196359045923No Hit
TTACGT270820.11335393197759543No Hit
GATTCA267340.11189734943833675No Hit
TTCTAC266170.11140763634324116No Hit
TCAATG265810.11125695539090406No Hit
ATTGAA262910.11004313660818851No Hit
AATTGC260190.1089046583016415No Hit
CTGTAT246960.10336713330325294No Hit
ATTCGA240610.10070928872730682No Hit
GTCTGC239890.10040792682263261No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)