FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26765135
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCGG4932111.8427368290875423No Hit
TGTGTG4417931.6506287003596285No Hit
CATAAC4172291.5588525893854075No Hit
GCATAA4108241.534922203829721No Hit
CTATTA3685871.3771161625002077No Hit
TCTCTG3552551.3273050929875752No Hit
CGTCTC3410681.274299569197017No Hit
TATAAG3094541.1561832211942888No Hit
CGCTTG2822811.0546593544176035No Hit
GACTAA2278400.8512566815000187No Hit
ACCGCT2225730.8315780959072314No Hit
CAAGCT2166490.8094448243956177No Hit
CTACCG2165960.8092468055924247No Hit
AGGTGT2072750.7744216496572874No Hit
TGGCAT2069080.7730504628502715No Hit
CGTACT2035460.7604893455609322No Hit
CTCCGC1977540.738849252955384No Hit
TACTCG1969490.7358416088691501No Hit
GTCTCA1927160.7200262580405441No Hit
GGTAAT1893340.7073904166745282No Hit
TCTCGC1887590.7052420994700755No Hit
AACTCT1833930.6851936296977392No Hit
ACCTGC1833670.6850964883980596No Hit
GGCTAC1810040.6762678387387174No Hit
GACCGA1769190.6610054460775184No Hit
GATCCT1767990.6605571016174587No Hit
CACCTA1745140.6520198758571553No Hit
TTAGCG1736290.648713335464215No Hit
AGACGG1729410.6461428272265394No Hit
AGAGGT1719080.642283328666192No Hit
TGACCA1704580.6368658331071374No Hit
CGTCAG1662750.621237292470223No Hit
TTCACA1651240.6169369218574836No Hit
AGATGC1633710.6103873565367781No Hit
CAAGGA1628750.608534199435198No Hit
TGCGAC1613180.6027169300659234No Hit
TATGGT1604260.5993842362461463No Hit
CATTAA1586230.5926478607337493No Hit
GCCAAT1564590.5845627156373394No Hit
GGATCG1559210.5825526379747383No Hit
CTTCAC1547830.578300838011839No Hit
AGTAGG1545360.5773779956648827No Hit
TATTCT1533750.5730402630138051No Hit
GGATTC1526340.5702717359729365No Hit
CGATGT1525160.5698308639205444No Hit
GCTCAA1512950.565268959039437No Hit
GGAGGC1503450.5617195653972976No Hit
GATTCA1497350.5594404810586608No Hit
ACGCTA1493800.5581141286976509No Hit
TAGAGC1481440.553496180759036No Hit
TGACAC1480600.5531823396369941No Hit
ACCAGG1468330.5485980175328837No Hit
TTCCAT1462370.5463712400479205No Hit
CTATGC1455740.5438941369060907No Hit
GCGCAG1455740.5438941369060907No Hit
CCTGCT1443400.5392836613751435No Hit
AGGCTT1442420.5389175133994281No Hit
TTAGGC1438740.5375425903885783No Hit
GAACCG1435770.5364329378499305No Hit
CCTGAC1426780.5330740906033166No Hit
TGATAA1411980.527544508929247No Hit
TTCTGT1410370.5269429801120001No Hit
ATCAGC1410320.526924299092831No Hit
CTTAGC1406260.5254074003362956No Hit
CGAGAG1401220.5235243536040449No Hit
CCTTAT1400270.5231694142398311No Hit
GAATAC1393070.5204793474794728No Hit
TCTTCA1387000.5182114717523375No Hit
GACCAT1374130.5134029774181972No Hit
AGTTCC1370780.5121513491338638No Hit
TTACGT1369620.5117179494891395No Hit
ATGTGA1368120.5111575189140649No Hit
ATCCAC1364750.5098984182220638No Hit
GTTACA1363710.5095098530233455No Hit
CAGATC1362020.5088784345754281No Hit
GTACTT1360790.5084188815038668No Hit
AATTGC1357710.507268130723047No Hit
TAAGTC1348250.503733681896243No Hit
GGCGTT1345950.5028743550144619No Hit
CTAGTT1340110.5006924119755047No Hit
AGTCAA1339330.5004009880764659No Hit
TATTGG1335660.49902980126944996No Hit
ACTAAT1333770.49832365874485596No Hit
ATTGCT1329690.496799287580653No Hit
GTACGC1327530.49599226755254555No Hit
AAGTTG1313180.49063081505099826No Hit
TCAATG1310520.4896369848311992No Hit
GGTACC1305290.48768295022610575No Hit
ACAGTG1296540.4844137718715037No Hit
CCAGCG1296210.4842904771449873No Hit
GGTGGT1291370.4824821544894132No Hit
TACAGT1287260.4809465747137087No Hit
TAGCTT1283090.4793885777150012No Hit
TTCGTT1279070.4778866237738012No Hit
ATTCAT1271890.4752040294211107No Hit
GGCAGG1265750.4729100002671386No Hit
GCACAT1265670.47288011063646795No Hit
GAGCCA1260820.47106805177706No Hit
AGGTCA1254970.46888237253426895No Hit
TAATGA1243980.46477628452088887No Hit
TCGGAC1242730.46430925904166No Hit
AAGGCG1240380.4634312511407097No Hit
GAGAAG1239400.46306510316499433No Hit
AAGCAT1238510.46273258102378334No Hit
TACGGC1228720.45907483747046296No Hit
ACTTGA1227740.4587086894947475No Hit
AGTTAG1226440.45822298299634956No Hit
AGTGCA1222760.45684805998549977No Hit
GATCAG1217590.45491644260340924No Hit
AACAAT1199860.44829215320602717No Hit
TCCTCC1193800.44602801368272565No Hit
TCCAGA1177730.4400239341217595No Hit
GAGTGA1172570.4380960529435028No Hit
CGAGCC1158230.4327383366457894No Hit
CTCGTC1151710.43030233174613164No Hit
ACGTAA1141420.4264577780011197No Hit
TCGTGG1133590.42353233039923016No Hit
AGAACG1125860.42064424483567897No Hit
ATTCGA1120100.4184921914273924No Hit
TACGAA1114750.41649332237629294No Hit
TTGAAT1114120.41625794153476153No Hit
AATGTA1111810.4153948784491466No Hit
GTCTGC1108940.41432258794883714No Hit
ATCCGG1107760.4138817158964451No Hit
GAAGAT1107610.4138256728389377No Hit
TTCTAC1100380.41112439746707796No Hit
GTCGAG1098190.41030616882746906No Hit
GCCATA1082670.4045075804773635No Hit
TGCGGA1076950.40237047188441233No Hit
CGAATT1071310.40026325292213166No Hit
CCGCTC1070800.4000727065266063No Hit
GGCCAG1063190.39722945540906107No Hit
GCCTGG1055200.3942442285458302No Hit
CCGTCG1048880.39188294772284915No Hit
ATACCT1048440.3917185547541606No Hit
ATTGAA1048010.39155789798930585No Hit
CAGTCC1037860.38776565109796757No Hit
CAGTAG1020530.3812908098539387No Hit
CTGTAT1012690.3783616260482153No Hit
ACACGA1010820.37766295593128896No Hit
GCGTAT1000010.3736241195869178No Hit
AGCCTA997150.3725555652904422No Hit
AACCTT994300.37149074719780045No Hit
ACTAGC991860.3705791134623457No Hit
CAACGG970230.3624977045697696No Hit
CGTGAA970100.36244913391992983No Hit
TCAAGC947490.35400157705163826No Hit
GCTGTC945270.3531721398005278No Hit
CCATGA944820.3530040106280054No Hit
GGCCGT941350.3517075478976661No Hit
CCGACT939080.35085942962738653No Hit
CAGCCT935940.34968626162356364No Hit
CGACAA935630.3495704393047149No Hit
TTAGTA934110.3490025363219726No Hit
CAGGAC932430.348374854077889No Hit
AAGCGC920930.34407821966898355No Hit
CCGGAA919640.3435962493744194No Hit
GTGGTG919220.34343932881339845No Hit
ATGCCG914790.3417841905150114No Hit
CTGTTC900160.3363181243061169No Hit
AACATA899080.3359146142920632No Hit
ATGGCC864350.32293877837716867No Hit
AACTAG852970.31868697841426913No Hit
ACAGCC840530.3140391408449836No Hit
GAGCAC827630.3092194378993418No Hit
CCTCGG825330.3083601110175607No Hit
ACGAAC818890.3059539957485736No Hit
CATACG815210.30457907273772394No Hit
TGCATA814480.3043063298578542No Hit
GTAAGA809910.3025988847057936No Hit
CGAAGC795240.2971178736815637No Hit
GCTTCT778470.2908522598522294No Hit
TGTGAT770940.2880388983653548No Hit
AAGAGG764800.28574486921138265No Hit
GGACTA762220.2847809286222543No Hit
CACATT758900.28354050894942245No Hit
GACTTG757950.2831855695852085No Hit
GAGTCG755990.28245327363377765No Hit
CCACTT752470.28113812988426923No Hit
CGTTGC746140.27877311285745426No Hit
AGGACT730440.2729072728383399No Hit
GACACG725430.2710354347175906No Hit
TCCACG719600.26885722788246724No Hit
CTGGAG695210.2597446267317538No Hit
TCATGT675480.2523730965676056No Hit
CATGAG669130.250000607133123No Hit
GAGATA668580.24979511592226228No Hit
GCGGCT662600.24756086602963143No Hit
CTGAGG658370.245980451807921No Hit
GTTGGC612400.22880512278380064No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)