FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22079200
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCGG4072731.8446003478386899No Hit
TGTGTG3586891.6245561433385267No Hit
CATAAC3447781.5615511431573608No Hit
GCATAA3379771.5307483966810391No Hit
CTATTA3062581.3870883003007355No Hit
TCTCTG2898131.3126064350157614No Hit
CGTCTC2802791.2694255226638647No Hit
TATAAG2560871.1598563353744702No Hit
CGCTTG2299091.041292256965832No Hit
GACTAA1842180.8343508822783434No Hit
ACCGCT1831080.8293235262147178No Hit
CTACCG1794420.8127196637559332No Hit
CAAGCT1791250.8112839233305554No Hit
AGGTGT1711650.7752318924598717No Hit
CGTACT1685770.7635104532772927No Hit
TGGCAT1663520.7534330954020073No Hit
CTCCGC1640120.742834885321932No Hit
TACTCG1625810.7363536722345012No Hit
GTCTCA1584810.717784158846335No Hit
GGTAAT1562670.7077566216167253No Hit
TCTCGC1554460.7040381897894852No Hit
ACCTGC1519490.6881997536142613No Hit
AACTCT1513290.6853916808580022No Hit
GGCTAC1476140.6685658900684808No Hit
GATCCT1457930.6603183086343708No Hit
GACCGA1440400.652378709373528No Hit
CACCTA1438740.6516268705387877No Hit
TTAGCG1437250.650952027247364No Hit
AGAGGT1418480.6424508134352693No Hit
AGACGG1403560.6356933222218196No Hit
TGACCA1381370.6256431392441755No Hit
TTCACA1367220.6192343925504548No Hit
CGTCAG1363250.6174363201565275No Hit
AGATGC1345900.6095782455886083No Hit
CAAGGA1342090.6078526395883909No Hit
TGCGAC1334670.6044920105800935No Hit
TATGGT1327920.6014348345954563No Hit
CATTAA1294060.5860991340265952No Hit
GGATCG1284760.5818870248922062No Hit
CTTCAC1284400.5817239755063589No Hit
GCCAAT1282110.5806868002463857No Hit
AGTAGG1277360.5785354541831226No Hit
TATTCT1275570.5777247364034929No Hit
CGATGT1253220.5676020870321388No Hit
GGATTC1252060.5670767056777419No Hit
GCTCAA1247100.5648302474727345No Hit
GATTCA1227180.5558081814558499No Hit
TGACAC1224140.5544313199753614No Hit
TAGAGC1223420.5541052212036668No Hit
ACGCTA1220700.5528732925105982No Hit
GGAGGC1211260.548597775281713No Hit
ACCAGG1205910.5461746802420377No Hit
GCGCAG1203860.5452462045726294No Hit
TTCCAT1201860.5443403746512555No Hit
CCTGCT1201350.5441093880213052No Hit
CTATGC1192880.5402731983042864No Hit
AGGCTT1186950.5375874125874125No Hit
CCTGAC1184900.5366589369180043No Hit
TTAGGC1168310.5291450777202072No Hit
GAACCG1167710.528873328743795No Hit
TTCTGT1167210.5286468712634516No Hit
TGATAA1166200.5281894271531578No Hit
ATCAGC1162460.5264955252001884No Hit
CGAGAG1158170.5245525200188412No Hit
CTTAGC1157660.524321533388891No Hit
TCTTCA1146770.5193892894670097No Hit
CCTTAT1141600.5170477191202579No Hit
AGTTCC1135360.5142215297655712No Hit
GAATAC1135010.5140630095293307No Hit
GACCAT1134220.513705206710388No Hit
ATCCAC1131480.5124642197181057No Hit
GTTACA1125220.5096289720642052No Hit
ATGTGA1123060.5086506757491214No Hit
TTACGT1122270.5082928729301786No Hit
CAGATC1120050.5072874017174535No Hit
GTACTT1119710.50713341063082No Hit
TATTGG1109050.5023053371498968No Hit
AATTGC1107480.5015942606616182No Hit
TAAGTC1106620.5012047537954274No Hit
AGTCAA1106270.501046233559187No Hit
ACTAAT1105120.5005253813543968No Hit
CTAGTT1104570.500276278126019No Hit
GGCGTT1102210.49920739881879783No Hit
ATTGCT1087080.4923547954636037No Hit
GTACGC1080420.4893383818254285No Hit
AAGTTG1076060.4873636725968332No Hit
GGTACC1072670.4858282908801043No Hit
TCAATG1069570.4844242545019747No Hit
ACAGTG1069270.4842883800137686No Hit
CCAGCG1066680.4831153302655894No Hit
TACAGT1065580.4826171238088336No Hit
TAGCTT1055040.4778434001231928No Hit
GGTGGT1052930.4768877495561433No Hit
ATTCAT1047220.4743016051306207No Hit
GGCAGG1047140.4742653719337657No Hit
TTCGTT1044530.4730832638863727No Hit
GAGCCA1041900.4718920975397659No Hit
GCACAT1041260.4716022319649263No Hit
AGGTCA1039180.4706601688466973No Hit
TCGGAC1015990.4601570709083663No Hit
TAATGA1015160.459781151490996No Hit
GAGAAG1013040.4588209717743396No Hit
ACTTGA1012230.45845411065618313No Hit
AAGGCG1011160.45796949164824813No Hit
TACGGC1009620.45727200260879014No Hit
AAGCAT1009570.4572493568607558No Hit
GATCAG999070.45249374977354256No Hit
AGTTAG998270.45213141780499294No Hit
AGTGCA997960.45199101416717996No Hit
TCCTCC983910.4456275589695279No Hit
AACAAT983730.4455460342766043No Hit
TCCAGA975920.4420087684336389No Hit
GAGTGA965310.43720334070075006No Hit
CGAGCC949580.43007898836914377No Hit
ACGTAA947020.4289195260697851No Hit
TCGTGG942490.4268678212978731No Hit
CTCGTC939050.42530979383310985No Hit
AGAACG927330.42000163049385847No Hit
TACGAA916630.41515544041450775No Hit
TTGAAT916630.41515544041450775No Hit
ATTCGA916600.4151418529656872No Hit
GAAGAT916550.41511920721765283No Hit
AATGTA908930.41166799521721803No Hit
GTCTGC907590.4110610891698975No Hit
ATCCGG905180.40996956411464186No Hit
TTCTAC904510.40966611109098156No Hit
GTCGAG900970.4080627921301497No Hit
CGAATT884240.40048552483785643No Hit
GCCATA881600.3992898293416428No Hit
TGCGGA875920.39671727236494075No Hit
CCGCTC868570.3933883474038914No Hit
ATACCT864120.39137287582883434No Hit
ATTGAA859340.3892079423167506No Hit
GGCCAG859250.38916717997028877No Hit
GCCTGG856790.3880530091669988No Hit
CCGTCG853230.38644063190695316No Hit
CTGTAT842580.3816170875756368No Hit
CAGTCC840690.3807610782999384No Hit
ACACGA839280.38012246820536977No Hit
CAGTAG837200.37918040508714085No Hit
GCGTAT813760.36856407840863803No Hit
CAACGG809310.3665486068335809No Hit
ACTAGC809110.36645802384144355No Hit
AGCCTA808640.3662451538099206No Hit
AACCTT806420.3652396825971955No Hit
CGTGAA795140.3601308018406464No Hit
CCATGA783830.3550083336352766No Hit
TTAGTA780710.35359523895793327No Hit
CAGGAC775190.3510951483749411No Hit
CCGACT772530.3498903945795137No Hit
GGCCGT772050.349672995398384No Hit
TCAAGC770830.3491204391463459No Hit
GCTGTC770380.34891662741403673No Hit
CGACAA763490.34579604333490344No Hit
CAGCCT757870.3432506612558426No Hit
CCGGAA757200.34294720823218233No Hit
AAGCGC756410.3425894054132396No Hit
GTGGTG746840.3382550092394652No Hit
ATGCCG741500.3358364433493967No Hit
AACATA736220.3334450523569695No Hit
CTGTTC734960.33287437950650384No Hit
AACTAG704810.31921899344179133No Hit
ATGGCC698430.3163293959926084No Hit
ACAGCC692090.313457915141853No Hit
CCTCGG685490.31046867640131887No Hit
GAGCAC673630.30509710496757125No Hit
ACGAAC671820.3042773288887279No Hit
GTAAGA668290.3026785390775028No Hit
TGCATA667790.3024520815971593No Hit
CGAAGC644230.291781405123374No Hit
CATACG642540.29101597883981306No Hit
GCTTCT640020.28987463313888184No Hit
GGACTA630050.28535907098083263No Hit
TGTGAT629250.28499673901228306No Hit
AAGAGG628680.2847385774846915No Hit
CACATT625600.2833435994057756No Hit
GAGTCG621890.28166328490162684No Hit
GACTTG615830.27891862023986375No Hit
CCACTT615510.2787736874524439No Hit
CGTTGC606260.27458422406608934No Hit
TCCACG594180.2691130113409906No Hit
AGGACT593690.268891083010254No Hit
GACACG591930.2680939526794449No Hit
CTGGAG577840.26171238088336535No Hit
TCATGT549470.2488631834486757No Hit
GAGATA549090.24869107576361463No Hit
CATGAG543090.24597358599949276No Hit
CTGAGG542530.24571995362150803No Hit
GCGGCT536520.24299793470777928No Hit
GTTGGC493580.22354976629588028No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)