FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26191708
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAAG9101763.4750540132777905No Hit
GCATAA5901212.253083304074709No Hit
CGTCTC5107241.9499453796598527No Hit
CATAAC4953071.891083239015951No Hit
TTCTGT4374461.6701698109951442No Hit
TGATAA4355601.6629690587570694No Hit
CTACCG4276751.6328641110385012No Hit
CGTACT4037611.5415604053007923No Hit
CTAGTT3961681.5125703142383842No Hit
GCTCAA3309691.2636403857281855No Hit
ATTCAT3301821.2606356179596994No Hit
GTCTGC3168581.209764556019027No Hit
AGGTGT3040111.1607146811502327No Hit
TACTCG2982861.1388566182854511No Hit
AATCGG2982201.138604630137141No Hit
ATTGAA2945681.1246612859306464No Hit
TTCACA2880741.0998671793378272No Hit
TCTTCA2806851.0716559607338323No Hit
AGGTCA2770671.0578424286037398No Hit
ATCAGC2735741.0445061467545378No Hit
CTATTA2682711.0242592808380424No Hit
ATGTGA2569980.9812189415062202No Hit
GACCAA2560760.9776987434343725No Hit
GGTAAT2521310.9626367245694706No Hit
GCGCAA2434430.9294659210464625No Hit
AGAACG2388280.9118458406759881No Hit
CTGTAT2371190.9053208748356542No Hit
AGATGC2289130.8739903483957595No Hit
CAAGCC2257360.8618605552566484No Hit
AGTAGG2230170.85147940714672No Hit
ATACCT2044280.780506563374943No Hit
ACTAAT2042440.7798040509614722No Hit
ACTAAA2016980.770083417240296No Hit
AGGCTT1985320.7579956221259033No Hit
CACCTA1904580.7271690719826289No Hit
GTTACA1875370.7160166874187816No Hit
TCTCGG1838240.7018404450752124No Hit
TGCGAA1815510.6931621259675008No Hit
GACCAT1779440.6793905918621268No Hit
CCAGCC1694710.6470406588222501No Hit
ACGTAA1655900.6322229921011643No Hit
CTCCGC1654770.6317915578472393No Hit
TATGGG1640380.6262974526136288No Hit
ACCTGC1585120.6051991721960248No Hit
TCGTGG1566100.597937331921996No Hit
GAGTGA1499220.5724025328932347No Hit
TGCGAC1494620.5706462518595581No Hit
TACGGG1471810.5619373887338696No Hit
CTTAGC1394920.5325807694557377No Hit
CAACGG1382030.5276593645591956No Hit
TCCAGG1345930.5138763764470801No Hit
CTCCGG1342060.5123988095774434No Hit
CCGGAA1316720.5027239918832327No Hit
ATACCC1287570.4915945153328679No Hit
CCTGCC1252720.4782887775016429No Hit
GTCTCA1216110.46431107127492416No Hit
CCTCGG1164600.4446445416999915No Hit
AGAGGG1163890.4443734635404457No Hit
GGATCG1159640.4427508125854182No Hit
AACTAG1136620.43396177141254016No Hit
ACAGCC1135230.43343106910018997No Hit
GGCTAC1088360.4155360925679226No Hit
TGACAC1062650.4057200087905684No Hit
TCTCGC1061820.40540311460405715No Hit
GAGTCG1048770.4004206216715611No Hit
CTTCAC1035730.39544194674131217No Hit
AGAGGT1019230.38914224303355854No Hit
TATTGG1005790.3840108480134247No Hit
ACCTGG1005650.383957395981965No Hit
AGTTCC1002330.38268981923592005No Hit
GGATTC996800.3805784639932608No Hit
TGACCA995990.3802692058112438No Hit
CCAGCG987740.37711935395736695No Hit
CGAGAG984620.37592813725626445No Hit
TATTCT978050.3734197097799044No Hit
AACTCT975230.3723430331462156No Hit
CGATGT969850.3702889479372632No Hit
AAGCGG968890.36992241972153933No Hit
GGTACC953540.3640617862722049No Hit
GCACAA932740.35612034159818823No Hit
GTACTT932400.35599052952178606No Hit
CGCTTG927570.35414643443642546No Hit
GGCAGG925000.35316520785891475No Hit
AAGAGG913790.3488852273398894No Hit
GCCAAA908090.34670896605902907No Hit
CCATGA901410.34415854055795064No Hit
GTCGAA894730.3416081150568722No Hit
AGTCAA878690.3354840394524862No Hit
ATCCAC877040.3348540690817109No Hit
TTAGTA875240.3341668286772287No Hit
CAAGGG856520.32701952847061366No Hit
CATAAA850940.32488908321671883No Hit
TTCACC833270.3181426732460518No Hit
GCACAT827460.31592441394047305No Hit
CGAGCC827190.3158213278798007No Hit
GGCCGT827180.3158175098775536No Hit
GAACCG800140.30549363180133193No Hit
GATCCT780340.2979339873520276No Hit
TAGCTT776180.29634569841722425No Hit
TTAGCG762140.290985223262263No Hit
CTGTAA746210.28490314568259545No Hit
ACCGCC734960.28060789315458157No Hit
TGTGTT725550.2770151530400385No Hit
CTTAGG711010.27146377777272107No Hit
TATGGT703070.26843228398850505No Hit
AGTTAG696070.26575968241551867No Hit
CGAATT674870.2576655176516171No Hit
TTCCAT662820.25306482494383337No Hit
GATCAG660820.25230122449440867No Hit
CAAGCT659450.25177815818655275No Hit
GATTCA656040.2504762194202837No Hit
CCGGGA643100.245535724512506No Hit
GGCCGG624400.23839606031038524No Hit
GAGCCA608950.2324972468385796No Hit
CAGATC606500.23156183628803437No Hit
ATTCAA597490.22812181626337616No Hit
CAGTCC596820.22786601011281893No Hit
ACCGGC587040.22413200391513222No Hit
TACGAA579850.22138686029945048No Hit
TCTCTT577660.22055071780733046No Hit
GCGCAG572970.21876007475342957No Hit
CGACAA565720.21599202312426513No Hit
TTAGGG559700.21369358577149686No Hit
TAGAGC558790.2133461475670086No Hit
AATTGC551970.21074227003447044No Hit
CACCTT549380.20975340745246548No Hit
GATCCC543250.20741297207497886No Hit
TCTCTG540650.20642029149072677No Hit
TGTGTG531550.20294590944584445No Hit
CGTCAG528560.20180432677395455No Hit
TACAGG521440.19908590917400273No Hit
AGACGG516880.19734490014931444No Hit
CCTGGC503490.19223259514041618No Hit
CATTAA495380.1891361953179991No Hit
CCTGAA487450.18610851953603025No Hit
CTACCC484000.18479130876077268No Hit
TTCTAC479980.18325647185742908No Hit
CACATT479130.1829319416664236No Hit
GGACTA477480.1823019712956482No Hit
ATGTGG476820.18204998314733808No Hit
CGTACC474310.1810916645833101No Hit
GCCTGG469500.17925520550244375No Hit
ACTTGA465990.17791508671370346No Hit
TTAGCC465060.17756001250472095No Hit
GAAGAA457740.17476523485982662No Hit
ACACGG448830.17136339485763966No Hit
TTAGTT448740.17132903283741557No Hit
ATTGCC438780.16752630259928067No Hit
GACTAA435910.16643053595435625No Hit
TAGAGG430030.16418555063304768No Hit
CCATGG429250.16388774645777207No Hit
TCCAGA426750.1629332458959912No Hit
GTCGGA425590.1624903576353249No Hit
ACAGTT425470.16244454160835942No Hit
AGTGCA424070.16191002129376214No Hit
CTATGC418020.1596001299342525No Hit
GACCGG399030.15234974366696513No Hit
GTAAGA396990.15157087120855195No Hit
TGGCAT392660.14991767623554753No Hit
TCGGAA391780.14958169203780067No Hit
GAGATA391230.14937170191420887No Hit
GAATAC390150.14895935767151955No Hit
TATAAA378460.14449611304463228No Hit
AAGCGC373050.14243057382893853No Hit
TTAGGC367450.14029249257054943No Hit
TGCGGA367190.1401932245121242No Hit
ACAGTG365110.13939908004472257No Hit
TAATGA362790.13851330352338992No Hit
TACAGT360820.1377611570807066No Hit
ATTCGA357170.13636758626050657No Hit
GCCATA355130.1355887138020934No Hit
CCTGAC343330.1310834711504878No Hit
CCGTCG335140.12795652731009372No Hit
GAGCCC334610.1277541731909962No Hit
CCACTT327310.12496703155059607No Hit
CAGGAA324780.12400107698207388No Hit
GTACGC317690.12129411338886338No Hit
AGTGCC314870.12021743675517459No Hit
ACGCTT314160.1199463585956288No Hit
GAAGAT312870.11945383630574989No Hit
TTACGT312550.11933166023384194No Hit
ATCCGG311170.1188047759237389No Hit
TCCACG310410.11851460775295754No Hit
GGCGTT308410.11775100730353286No Hit
CAGGAC300730.11481878157774209No Hit
CGCTTT299290.11426898925415631No Hit
CGTGAA297530.11359702085866259No Hit
GGTAAA297040.11340993874855355No Hit
CTGAGG294380.11239435015081872No Hit
TTCGTT291930.1114589396002735No Hit
ACACGA290170.11078697120477977No Hit
GGCCAG289580.11056170907219949No Hit
CGAAGC281170.10735076918236872No Hit
GTCGAG271800.10377330107681408No Hit
ACCAGG271250.1035633109532223No Hit
AACCTT269730.10298297461165953No Hit
GCCATT269580.10292570457795269No Hit
TACGGC268920.10267371642964254No Hit
CCGTCC267390.10208956208583266No Hit
ACTTGG266980.10193302399370059No Hit
GACTTG266690.10182230192853402No Hit
AAGTTG265000.10117705954877017No Hit
GTGGTT264390.10094416141169563No Hit
CAGTAG264220.10087925537349454No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)