FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20866105
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACA2028840.9723137116390433No Hit
GGACTA2022200.9691315173579353No Hit
ACTAAT2016140.9662272858302975No Hit
GGCTAC1955990.9374006313109227No Hit
TCTTCA1938900.9292103150060828No Hit
GAGAAG1921050.9206557716449716No Hit
GCTCAA1908680.9147274970580278No Hit
TCAATG1886840.9042607616514917No Hit
GAGCAC1875450.8988021482686873No Hit
ATTCAT1871060.8966982577725933No Hit
ACAGCC1858820.8908322851821171No Hit
AACATA1833210.8785587918780242No Hit
AGTGCA1830610.8773127519486748No Hit
CGAATT1800830.8630408022963557No Hit
CCTTAT1786930.8563792811356025No Hit
TACAGT1735190.8315830865415467No Hit
TAGCTT1730660.8294121015877185No Hit
AACCTT1680550.8053970781801395No Hit
CTGTTC1654960.7931331697985801No Hit
CTTAGC1653950.7926491312106404No Hit
CCTGCT1623100.7778643882027816No Hit
GAAGAT1618410.7756167238686856No Hit
TCTCGC1607220.7702539597112159No Hit
GCCAAT1606670.7699903743415458No Hit
TGCATA1603830.7686293153417947No Hit
TTCTAC1598900.7662666319372974No Hit
ACCAGG1568900.7518892481371103No Hit
CTTCAC1561290.7482421851131296No Hit
ATGGCC1549420.7425535335895224No Hit
GAATAC1532450.7344207268198832No Hit
TGTGAT1531150.7337977068552085No Hit
GTCTCA1527270.7319382318837176No Hit
GAGATA1525110.7309030602501041No Hit
GTAAGA1515690.7263885617368454No Hit
ATTGAA1506970.7222095355122577No Hit
TTACGT1506370.721921987836254No Hit
ACTTGA1476480.7075973211100011No Hit
GAGTCG1473050.7059535068955131No Hit
TCATGT1444910.6924675208909378No Hit
CCTCGG1431900.6862325287829233No Hit
GATCAG1401720.6717688806799352No Hit
GCACAT1397770.6698758584795772No Hit
CATGAG1388540.6654524167303865No Hit
AACTCT1379600.6611679563579307No Hit
CAGATC1374980.6589538392527019No Hit
TCGTGG1363080.6532508103452944No Hit
TGATAA1359860.6517076378174077No Hit
ATCCGG1358800.6511996369231344No Hit
CGTCAG1358250.6509360515534643No Hit
GTCTGC1356530.6501117482155869No Hit
GGTAAT1355730.6497283513142487No Hit
ATGCCG1334270.6394437294358483No Hit
GACTTG1328340.6366017999046779No Hit
CAGCCT1322800.6339467763629101No Hit
CAGTAG1316470.6309131483810707No Hit
GCTTCT1303890.6248842321075256No Hit
CTGAGG1303600.6247452507307905No Hit
CTGTAT1282680.6147194217607934No Hit
TCCAGA1280500.6136746652046465No Hit
CGACAA1276330.6116762088564205No Hit
AAGGCG1270290.6087815622513162No Hit
AAGAGG1263700.6056233302765418No Hit
CAGTCC1260150.603922006526853No Hit
CTGGAG1256050.6019570974074941No Hit
AGTTAG1251030.5995512818515961No Hit
GCTGTC1250250.5991774698727913No Hit
TCCTCC1242790.5956022937678115No Hit
TTAGTA1237700.5931629309830464No Hit
GACACG1235090.5919120985924301No Hit
GGTACC1227350.5882027335719819No Hit
GTGGTG1212460.5810667587458225No Hit
CTATTA1211100.5804149840135473No Hit
TACGGC1194580.572497838000911No Hit
AGGCTT1187130.5689274543571979No Hit
GGCCAG1184920.5678683204172508No Hit
TTGAAT1179320.5651845421078826No Hit
CCGTCG1164590.5581252466619908No Hit
GGCGTT1162180.5569702634967091No Hit
AACTAG1159960.5559063370954953No Hit
TAGAGC1158100.5550149392998838No Hit
TCCACG1149170.5507352713886947No Hit
ATCCAC1147120.5497528168290152No Hit
TCAAGC1146370.5493933822340106No Hit
CCATGA1136500.5446632229637491No Hit
GAGCCA1119580.5365543785004436No Hit
CGTACT1116600.5351262250429584No Hit
GCGCAG1110890.5323897296596561No Hit
TGACCA1101040.5276691553119282No Hit
TGTGTG1097790.5261116054002412No Hit
CACATT1093300.5239597902914799No Hit
AGATGC1082530.5187983095072127No Hit
CGTTGC1081440.5182759312291393No Hit
ACGTAA1075980.5156592473775052No Hit
CAAGGA1075080.5152279258634997No Hit
CGTGAA1074250.5148301515783612No Hit
CCACTT1068220.5119402974345236No Hit
AGGACT1050030.5032228103903436No Hit
CATACG1045360.5009847309787812No Hit
AATGTA1033850.4954686080607761No Hit
GTACTT1031650.4944142665820957No Hit
GACCAT1030850.4940308696807573No Hit
CGTCTC1011230.4846280606754351No Hit
CCGGAA1010700.4843740602282985No Hit
CAGGAC997040.47782755813794664No Hit
CCAGCG987540.47327471993455417No Hit
GCCATA982170.4707011682343207No Hit
GGCCGT971580.4656259517528547No Hit
ACTAGC969050.46441345905237225No Hit
CATAAC943900.4523604189665489No Hit
GTTGGC940850.4508987182801965No Hit
AACAAT940110.45054407614645864No Hit
ACCGCT920200.4410022857644012No Hit
GACCGA908680.4354813703851294No Hit
CAACGG901220.43190619428014954No Hit
ACGAAC890610.42682139287615006No Hit
TATAAG887330.425249465580663No Hit
CTCGTC868980.41645529915621526No Hit
ACACGA864340.4142315971284531No Hit
GAACCG862350.41327789733637393No Hit
TAAGTC850370.40753652873883267No Hit
TTCTGT844420.40468501428512893No Hit
GATTCA842780.40389905063738535No Hit
AATTGC837640.40143572554628665No Hit
CGAAGC833350.39937975966285993No Hit
CCGCTC825710.395718319255079No Hit
AGACGG806960.3867324543799621No Hit
CTCCGC805650.3861046419540206No Hit
GCATAA791960.37954376247986865No Hit
GACTAA790340.37876738375465857No Hit
AATCGG786220.37679288971276625No Hit
GCCTGG779640.37363945019925854No Hit
TTCCAT763300.3658085684894234No Hit
TATTCT759700.36408328243340093No Hit
CGATGT757970.36325418663425685No Hit
TGGCAT747910.3584329705999275No Hit
CGAGCC745170.3571198362128437No Hit
TATGGT720440.3452680795002229No Hit
GGTGGT719760.3449421921340854No Hit
GTTACA708710.33964652243434984No Hit
AAGCGC706260.3384723694240013No Hit
GGATTC685040.3283027666160024No Hit
AGTAGG677370.3246269488244212No Hit
TAATGA658240.3154589704211687No Hit
GTCGAG657280.3149988941395627No Hit
TCGGAC652740.3128231167244677No Hit
GTACGC650770.3118790018549221No Hit
TACTCG650440.31172085063312005No Hit
GGCAGG649740.31138537834444907No Hit
TACGAA648550.3108150754537083No Hit
TCTCTG640240.30683254014105654No Hit
CGCTTG638920.3061999352538483No Hit
CTATGC636990.3052749902293696No Hit
AGTTCC634010.30384683677188434No Hit
CCTGAC632010.3028883445185386No Hit
ATCAGC627780.3008611334027122No Hit
TTCGTT626370.3001853963641034No Hit
ATTGCT625930.29997452806836733No Hit
TTAGCG623620.29886746951575294No Hit
ATTCGA619510.29689776793512734No Hit
ACAGTG617610.2959872002944488No Hit
TTAGGC611380.29300149692527666No Hit
CTAGTT606740.2907777948975144No Hit
AAGCAT590950.2832104985573493No Hit
GAGTGA589100.2823238932230045No Hit
CTACCG586680.2811641175964561No Hit
ATACCT581570.27871516988915757No Hit
CGAGAG581520.2786912075828239No Hit
AGTCAA568640.272518517471277No Hit
AGAGGT560760.26874205799309453No Hit
AGGTCA559290.2680375661868854No Hit
ATGTGA559010.26790337727141694No Hit
AGCCTA543320.2603840055439192No Hit
TGACAC540270.25892230485756684No Hit
AAGTTG534340.25608037532639655No Hit
ACCTGC534210.2560180733299291No Hit
TGCGAC530880.2544221837281083No Hit
GGATCG529510.2537656165345665No Hit
AGAACG524760.2514891974328702No Hit
CCGACT523060.25067447901752626No Hit
AGGTGT521640.24999394951765075No Hit
GGAGGC513030.2458676403669971No Hit
GCGTAT485140.23250146589408996No Hit
CAAGCT480520.2302873487888612No Hit
CACCTA475860.22805406183856544No Hit
TGCGGA472610.22649651192687853No Hit
GATCCT458740.21984936814992542No Hit
ACGCTA448000.2147022647494585No Hit
TATTGG435960.2089321413843168No Hit
GCGGCT356840.1710141878419571No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)