FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24463287
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGT9751273.9860833092462187No Hit
CTTCAC8941423.6550362181500797No Hit
GTCTCA8777573.5880583014048764No Hit
AACTCT7931143.2420581911171626No Hit
TCAATG7868143.216305314980771No Hit
GAGCAC7665623.133520037597564No Hit
TAGAGC6522382.66619117864251No Hit
CAAGGA6115622.499917529479992No Hit
GTACTT5944662.430033216713682No Hit
GCTTCT5496892.2469956715138077No Hit
TGTGTG4612631.8855315722699078No Hit
CGTTGC4519501.8474622809273342No Hit
AGGACT4291071.7540856222632715No Hit
AATGTA4286091.7520499187210614No Hit
ACTAGC3989661.6308765048621634No Hit
AGTTCC3741591.5294714892565338No Hit
GGCAGG3713971.5181811013376902No Hit
GACCGA3704511.5143140821591146No Hit
TTAGCG3658481.4954981315470812No Hit
CCTGAC3630321.4839870046899257No Hit
TGACAC3150991.2880484948731543No Hit
TTCGTT2609011.0665001804540821No Hit
CCTGCT2412970.9863637703306182No Hit
AAGTTG2238450.9150242156746966No Hit
GCACAT2098760.8579223225398941No Hit
TCGTGG2060250.8421803660317602No Hit
AAGAGG1891870.7733506948596074No Hit
TTAGTA1865200.7624486439618683No Hit
CCTTAT1816170.7424063659147685No Hit
AGGCTT1765660.7217590996663694No Hit
GGACTA1724810.7050606077588837No Hit
CCATGA1695750.6931815826712086No Hit
AGATGC1604710.6559666327750642No Hit
ATGGCC1578670.6453221106386889No Hit
ACTAAT1550450.6337864572328322No Hit
GAATAC1544440.6313297146045828No Hit
CGAATT1518240.6206197883383373No Hit
ATTCAT1455860.5951203532051927No Hit
CATAAC1378210.5633789114275608No Hit
ATGCCG1367290.5589150795639196No Hit
CAGTAG1317680.5386357115460404No Hit
GAGATA1313720.5370169593317529No Hit
GTAAGA1304020.533051833958372No Hit
TTCACA1286680.5259636613836889No Hit
GGCTAC1282570.5242835927976481No Hit
TCTTCA1256650.5136881237586756No Hit
GCTCAA1243740.5084108280297738No Hit
GGCCAG1202260.4914548073609242No Hit
GAGAAG1176220.48081028522454894No Hit
ATTGAA1173810.479825135518379No Hit
GGCGTT1166330.4767674924469471No Hit
TAGCTT1141660.46668299317258555No Hit
CTGGAG1080320.44160868488359717No Hit
GCCAAT1054190.43092737292417No Hit
TGATAA1050460.4294026391465709No Hit
GTCTGC1044150.4268232637748149No Hit
CTGTAT985790.402967107404659No Hit
ACTTGA983860.40217817008809975No Hit
ATCCAC975380.39871175120497915No Hit
AACAAT951760.3890564665328907No Hit
CACATT933850.38173529174554505No Hit
TTACGT928730.37964235958969866No Hit
GATCAG911630.3726522932098209No Hit
AACATA897390.36683132565137305No Hit
CAGATC894170.3655150675377352No Hit
TTCTAC878690.3591872179727933No Hit
TGTGAT851380.3480235505555733No Hit
CATTAA835280.34144225998738437No Hit
CGTCAG833780.3408290962698512No Hit
AGACGG819370.33493863682341624No Hit
TCATGT799480.32680808592892685No Hit
AAGGCG796470.32557767073574373No Hit
CAGCCT796350.32552861763834107No Hit
CTTAGC795540.32519750923087315No Hit
ACCTGC791060.3233661935945075No Hit
ACCGCT777040.3176351567146312No Hit
TCTCGC774760.3167031478639808No Hit
GACCAT764260.31241100184124887No Hit
ACCAGG758300.3099746980035839No Hit
TTGAAT733740.29993516406850806No Hit
TGACCA727830.2975192990214275No Hit
AACCTT723060.2955694383996721No Hit
GGTGGT722350.2952792075733731No Hit
CGTACT717010.2930963447389552No Hit
CAAGCT714710.29215616037207104No Hit
GCGCAG713080.29148985579901837No Hit
CACCTA708600.28965854016265274No Hit
CTGAGG706740.28889821715291164No Hit
CTGTTC703920.28774546936394935No Hit
TGCATA690420.28222699590615113No Hit
TATAAG679540.27777951507497745No Hit
GTGGTG675650.2761893771675082No Hit
CATGAG674580.2757519870490012No Hit
AATCGG673600.2753513867535462No Hit
GGTAAT665080.27186861683795804No Hit
CGTGAA662720.27090390592237257No Hit
TATTCT654260.2674456625554857No Hit
CCGGAA647360.26462510945483325No Hit
TTCTGT644490.2634519228752865No Hit
CCAGCG626590.25613483584605784No Hit
GCTGTC618510.25283192728761267No Hit
TCCAGA614460.2511763852502732No Hit
TCCTCC605390.24746878863825617No Hit
GACACG604530.24711724144020386No Hit
GGATTC594750.24311941400188783No Hit
CCACTT589210.2408547960051321No Hit
ATCCGG583050.2383367370051294No Hit
ACAGCC578670.23654629894993262No Hit
AGCCTA548430.2241849184044646No Hit
CCGACT525960.21499972591581826No Hit
CTCGTC524790.2145214582161424No Hit
AGTGCA500700.2046740489125603No Hit
CGATGT499470.2041712546641831No Hit
AGTCAA493970.20192298769989495No Hit
CGTCTC490880.2006598704417767No Hit
TCAAGC485720.19855058725346272No Hit
ATCAGC478340.19553382176319967No Hit
GGCCGT472750.19324876497585952No Hit
GATTCA467260.19100458576968826No Hit
AATTGC465000.1900807524352717No Hit
CTAGTT464190.18974964402780378No Hit
GTTGGC460530.18825352455702293No Hit
GAGTCG460180.1881104530229319No Hit
CCTCGG451310.1844846115732526No Hit
ATACCT447470.1829149124563678No Hit
GAAGAT438290.179162350505065No Hit
GCCTGG429540.1755855621527884No Hit
ACAGTG417400.17062302379888689No Hit
CATACG409850.16753676642063678No Hit
TTAGGC409260.16729558869174038No Hit
CCGCTC404320.16527623618199794No Hit
GTACGC402680.16460584385082838No Hit
TCTCTG400520.16372288809758068No Hit
GACTAA397650.16254970151803394No Hit
CTCCGC393350.16079196552777228No Hit
ACGAAC385860.1577302346982235No Hit
CTACCG376300.153822337938479No Hit
GACTTG376210.15378554811542702No Hit
TACGGC365500.1494075591722404No Hit
ATGTGA364260.14890067716574637No Hit
AACTAG363290.14850416462840826No Hit
TAAGTC362850.14832430327126522No Hit
AGGTCA360810.14749040061542018No Hit
AGTTAG350650.14333723836866238No Hit
CGCTTG350550.14329636078749353No Hit
CGACAA350120.14312058718846735No Hit
AAGCGC348900.14262188069820708No Hit
ATTCGA348350.14239705400177827No Hit
ATTGCT347070.14187382096281664No Hit
CAGTCC344660.14088867125664675No Hit
TGCGAC342370.1399525746478795No Hit
ACGTAA339060.13859952671118972No Hit
AGAACG337310.1378841690407344No Hit
GTCGAG330580.13513310782806906No Hit
TGGCAT325950.1332404758199501No Hit
CCGTCG324520.13265592640923518No Hit
CTATTA323700.1323207302436504No Hit
GGTACC321630.1314745643134547No Hit
TACTCG319440.13057934528585632No Hit
GGAGGC316940.1295574057566344No Hit
TCCACG310310.12684722212513797No Hit
GAGCCA306220.12517532905533094No Hit
TAATGA289780.1184550547111678No Hit
ACGCTA288620.11798087476960885No Hit
AGAGGT279860.11439999865921534No Hit
CTATGC278480.11383588803908484No Hit
GCCATA276900.11319002225661662No Hit
CAACGG275850.11276080765434343No Hit
CAGGAC265410.10849318818031281No Hit
AAGCAT261220.10678041752933691No Hit
AGGTGT257120.105104436701413No Hit
GCATAA255500.10444221988647723No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)