FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395205692
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGAGG46909211.1869568417045977No Hit
CGCGCGCT40327691.02042280301975No Hit
GTATGCCT37099620.9387420462557509No Hit
AATTGCTA35098390.8881043646506994No Hit
ATATTACA35000810.8856352706579945No Hit
CAGAGGAT34848710.8817866418786297No Hit
CTCACCTT33903770.8578765611503388No Hit
CTGTTCGC33625700.8508404782793463No Hit
CCAGATTC32153690.8135937981379074No Hit
CTGCTCAG31324470.7926118128885653No Hit
TAATGATT31087450.7866144296322535No Hit
AGCTGTGG30168070.7633511007225068No Hit
TCGCGGAT29009900.7340456017521124No Hit
GGAAGGCG28708090.7264088190308757No Hit
CTCTCTGC28687030.7258759319691175No Hit
TTGAGAGT28595450.7235586576521271No Hit
GACGCTCA28568020.7228645887013185No Hit
AACGCTGG28487880.7208367838993573No Hit
TCGGTAAT28283990.7156776982857829No Hit
CGACAGTT27672210.7001976580843375No Hit
GTCTGGCG27619600.6988664525611133No Hit
TGATAAGG27556790.6972771535891745No Hit
CCGACCGC27302140.6908336735190544No Hit
AAGACACT27242460.6893235738112801No Hit
GTTCGGAC26961920.6822249918404516No Hit
CCTTACTG26446810.6691910196475611No Hit
TCTGCGCA26391800.6677990862540512No Hit
GAGCCGTC26083390.6599953018895284No Hit
TCTCTATA26038580.6588614619447333No Hit
ACGTGCGC26036960.6588204706322904No Hit
GAGACCAT25880880.6548711348013682No Hit
GCCGTCCT25215000.638022187190563No Hit
CGTTCGCG25181040.6371628878260185No Hit
AGCATAGT25176280.6370424442166183No Hit
GAGAGCGG25139300.6361067289486306No Hit
GTTGTGTG25082100.6346593813734849No Hit
CCGGTCTT24650160.6237298829187916No Hit
AAGCGCAC24524550.6205515380077066No Hit
TATTAGAG24206920.6125144574081691No Hit
AGGAATAC23486520.5942859750107041No Hit
TAGCTGGT22855840.5783277028307579No Hit
TGCATCAA22738710.5753639297280162No Hit
ACTAAGTG22496280.5692296557307682No Hit
TCTACCGT22116890.5596298446025418No Hit
GGATCAGA22068150.5583965627701536No Hit
ACTCGGAA21660270.5480758612150759No Hit
TGGCTTCC21646940.5477385685021966No Hit
ACGCGACC21536430.5449423031083267No Hit
GTCCAGAG21510340.544282140551761No Hit
ATTATCGT21483230.543596168650324No Hit
CTCCTTCA21360040.5404790576751106No Hit
CTCTATAG21296890.5388811555882145No Hit
CCAATCTA21194220.5362832679039451No Hit
ATATGGTT21166470.5355811019037651No Hit
GATTATCT20945120.529980221033861No Hit
TGGCCTAT20895190.5287168282991228No Hit
CCAACAAT20882680.5284002842752579RNA PCR Primer, Index 36 (100% over 8bp)
CCGTACAC20801580.5263481883251823No Hit
CGCATATA20553880.5200805660460984No Hit
CTGGCCTC20511830.519016563152132No Hit
CCAGCCAA20365050.5153025478185673No Hit
AACGAGAT20297590.5135955885979496No Hit
ACTTCTAT20284850.5132732248198489No Hit
GCTCCAGA20230250.5118916657708462No Hit
TAACATTA20228700.5118524456879533No Hit
AACCTTCT20052260.5073879350907728No Hit
ACAGCACG19974380.5054173156999976No Hit
GGCCTGCT19905500.5036744258227941No Hit
GGATCTCT19866300.5026825372747921No Hit
TTCGTTCG19544020.49452779642657574No Hit
ATGTTGTG19483110.49298657368527976No Hit
CTCACGCC19469790.4926495340051934No Hit
ATTGAGTA19465540.49254199506822893No Hit
AACTCTAC19456520.4923137594890713No Hit
GGCACGTT19456440.4923117352267285No Hit
ACGAGTTC19446770.4920670525160351No Hit
TCAGGTAC19309320.48858911677820677No Hit
AAGTTACG19141100.4843325991367554No Hit
TGAGGCGG19101230.48332375739163186No Hit
CACTCTCG19095830.48318711968348876No Hit
TTGTACTC19081110.4828146554124023No Hit
CGAACGAG19069740.4825269571269232No Hit
CATCGAGC19025570.48140931128087094No Hit
GTGGCGAA19001170.4807919112662983No Hit
AGGATTGG18875250.4776057223386347No Hit
GCCTCATT18651480.47194360753285913No Hit
GGCGCCAT18643880.4717513026102873No Hit
TATAATCC18611090.4709216080825071No Hit
CTGATCCT18546520.4692877753390252No Hit
TTCGGAAG18495550.4679980671938298No Hit
GGTCAACT18408520.46579592279758963No Hit
CACGGCAA18382950.4651489179462526No Hit
GGTGCGGA18217130.4609531281750871No Hit
AGTTCGTC18108150.45819557679852446No Hit
CGCGTGAA18081710.45752655809420884No Hit
CAACCGGT17972520.4547636930289961No Hit
AGGTGTAA17880960.4524469247775915No Hit
CATAATAA17850530.4516769459889257No Hit
GCTCACGT17650880.44662514627952277No Hit
GCCACGAC17607660.44553153854879196No Hit
TATCTACT17535880.4437152691616597No Hit
GGCCAATA17454630.4416593777196913No Hit
AGCACAAC17405960.44042786711685317No Hit
GGAGAACC17384160.43987625562842353No Hit
CGCTGAAG17342860.4388312301939214No Hit
TATAACGG17292660.43756100557377603No Hit
TGTAGACG17160730.434222743937605No Hit
AGGCCGAC17136460.4336086333493395No Hit
TGCCTGAC16957930.4290912388984519No Hit
TACGTCAG16871300.42689921581392604No Hit
TATATAGC16738680.42354349491504795No Hit
AGAGTATT16707150.4227456825191677No Hit
GATATATG16581750.4195726512967329No Hit
TCGGCCAG16485090.41712683632097075No Hit
CTAACATA16445890.41613494777296883No Hit
TAGTCGTG16429560.41572174522223226No Hit
GTCAACGT16400130.4149770697128522No Hit
GATTGGAT16357040.41388675140842857No Hit
CGTGCCAC16341730.4134993582025635No Hit
GGATACTA16299090.41242042637381854No Hit
ATAACGCG16298230.4123986655536328No Hit
ACATTCCT16285990.412088953415175TruSeq Adapter, Index 27 (100% over 8bp)
ACCACCAA16251030.4112043507713447No Hit
ATCCTAAC16175290.40928788039824077No Hit
GTCAGATG16173650.40924638302021216No Hit
GGTTGCAG16023240.40544051678284027No Hit
CGTCCGAA16010930.40512903341483253No Hit
AACTAGTC15897070.4022480020353553No Hit
AACTACCT15894410.40218069531245515No Hit
GAGTGGCA15745720.3984183507154548No Hit
CAACGTAA15530160.39296397583261533No Hit
GCGCTGGC15528030.3929100798477366No Hit
CCTTAGCC15443430.39076942242016094No Hit
AGACAGAG15433790.3905254988078461No Hit
CTTCCTGT15375450.3890493054943146No Hit
ATCTGTCT15169410.3838358178302756No Hit
TTCACTTG15128160.3827920575597378No Hit
TGTACGCC15127910.38278573173991637No Hit
ACATCCAG15093300.381909985243836No Hit
CAATTAAG15037740.3805041350467189No Hit
CAGATTCA14988690.37926300919775213No Hit
GTTGGTCG14949790.3782787116335359No Hit
GTCATTAG14930290.37778529768746344No Hit
CAGCGTCT14755410.3733602602059689No Hit
AGATGCGA14513290.3672338302252995No Hit
CTATGGAG14438620.3653444343610314No Hit
ACCTGAAC14305830.3619844119046747No Hit
TCGTCTGG14201880.35935413602291943No Hit
ATCATGGA13909850.3519648193730975No Hit
ACCAGCGG13820000.34969131972927153No Hit
GAGTAATT13706580.34682142179268005No Hit
GCCTGTGA13689170.3463808917003149No Hit
TAATTGAT13655010.34551653167991314No Hit
GTCCTCAA13497760.34153759101222664No Hit
TCCTGGAA13488170.34129493256387616No Hit
CTACGGCC13276520.335939493503044No Hit
AGGCGTGC13026810.3296210116325956No Hit
GGTGAGAT12980500.32844921676887184No Hit
TGCCTAGA12944050.32752691223890573No Hit
CCGTGGCG12758580.3228339130297749No Hit
CAATATGG12638390.3197927118924188No Hit
CGCTACAA12270050.31047250200030013No Hit
CGGCCAAG12131690.30697154027832174No Hit
GAGGTGCG12095370.3060525251746627No Hit
GAAGCGAT12078600.3056281891810404No Hit
CGAACTGC11836320.2994977106756853No Hit
TTGTCACA11730430.2968183464321157No Hit
TCGAGCAA11693240.2958773174754781No Hit
CATCCTCA11660300.29504382745580493No Hit
GCAACAGC11417120.2888905759990926No Hit
AGTTCCGG11122680.281440278446192No Hit
TAAGCAAC11100560.2808805699083909No Hit
TGCTGCCG11058590.27981859127676734No Hit
CTCAGAAC10765180.27239435610153107No Hit
TTGGAACG10537200.26662571448996236No Hit
ATGCAACT10250670.25937556587621213No Hit
CAAGCACT10006880.2532068794191355No Hit
CATTAAGT9929970.25126080420926733No Hit
ATGCTAGG9476130.23977716393821574No Hit
AGCCATTG8994880.22759996078194136No Hit
CTGTAGCA7557340.19122548467748285No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)