FastQCFastQC Report
Tue 28 Mar 2023
EGAF00001836352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393101411
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATT45136931.1482260998549303No Hit
GTTCTAAT39670531.0091678353197262No Hit
TCCGCGAT36421310.9265118104600241No Hit
CCGCTGTA36007540.9159860278395184No Hit
GCCGGTTG35766190.9098463907574221No Hit
GTAGCACA35603870.9057171763751313No Hit
CGAATCGT33412560.849973036601489No Hit
TACGTACC32540320.8277843602041917No Hit
GAAGGTGA31976160.813432847230355No Hit
AGATGACT31797520.8088884728017421No Hit
TCTCGAGT31655870.8052850769340026No Hit
TTGTGTAC30896520.7859681785777157No Hit
GCCTGGTC30885160.7856791946239033No Hit
TCGTGTCA30665460.7800903060101202No Hit
TTAGGATC29409360.7481367193566242No Hit
TGGAAGAT29136530.741196271106745No Hit
ACGCATTA28294520.7197766074668198No Hit
CGGACATC27821990.7077560451697285No Hit
CTGTCATT27136310.690313217929406No Hit
TTAGCAGT26866510.6834498490263623No Hit
TAGGTCTC26830380.6825307477718516No Hit
TTGTGGCT26495090.674001396550571No Hit
GCCATCGC26136880.664888989676No Hit
TCCTCCTA25843830.6574341703393174No Hit
TCCGTATG25814400.6566855085646081No Hit
ATCTCAGT25750410.6550576843388639No Hit
TGCTTCTT25566910.6503896776905743No Hit
GTTAAGCC25226720.6417356767004838No Hit
CGTGTTAG25133870.6393736907751776No Hit
GCATAACG25105020.6386397834628988No Hit
CTCTCGAA24889490.6331569743462457No Hit
CGACTCTG24787500.6305624784440166No Hit
GACGATGT24642000.6268611434722121No Hit
TACAGCCT24502480.6233119320958123No Hit
AGACCATC24385250.6203297499738559No Hit
CCTGGCCA23783660.6050260653986815ABI Solid3 GAPDH Forward Primer (100% over 8bp)
ACTGAGGC23343510.5938292091248688No Hit
GCTTCTCA22602540.5749798745952607No Hit
CAGCTAAC22374470.5691780638253674No Hit
ATGGCGCT22319830.5677880917094953No Hit
ACATACTC22136370.5631211026103389No Hit
TTACCTAC22033650.5605080364364299No Hit
TTGACCGA21357280.5433020437568462No Hit
ATGTCAAC21172920.5386121598021941No Hit
TCCGGTCT21070210.5359993480155685No Hit
CTTCTAGA20965280.5333300622520534No Hit
CAGAGTGC20849420.5303827311879071No Hit
AGAACACA20630300.5248085970365546No Hit
AATTATTG20008770.5089976642185088No Hit
CCATTGAC19972580.5080770366402985No Hit
TGCTAACT19808870.5039124624256309No Hit
AGGCGGTT19659320.5001081006040958No Hit
AATGCGAA19520800.4965843279560246No Hit
AAGCCTGT19414030.4938682349323849No Hit
AATTCATT19172470.48772325571733954No Hit
GAGACTGA18854320.47962992429961027TruSeq Adapter, Index 15 (100% over 8bp)
CTAGTCCG18780780.47775916021832854No Hit
TGAGCTCC18743010.47679833944935907No Hit
GACAGTCG18719410.47619798546080516No Hit
GCAGATCT18686930.47537173556469375No Hit
AGAACGGT18684220.47530279661092345No Hit
TATAGGTG18672700.4750097424605785RNA PCR Primer, Index 39 (100% over 8bp)
GGAACCTG18537580.4715724614888243No Hit
GAGCTTAG18437120.4690168868409379No Hit
TTATCGTC18427890.4687820873784653No Hit
CTCGTATC18381780.4676091076152357No Hit
GTAATCTT18319620.46602783626233285No Hit
ACGCTATT18288270.4652303321292327No Hit
CAACAGCA18217200.4634224017069224No Hit
TGTTGCGT18193490.46281924945825237No Hit
TAACTATG18184150.46258165173566373No Hit
ACTTGTCC18044820.45903727371764635No Hit
CGTTGTCT18039790.45890931691415376No Hit
AACTTGAA17889250.4550797707515733No Hit
AGTCATGA17864700.4544552499711073No Hit
TAGCCACC17679330.44973967290084343No Hit
GCAGTGGT17495750.44506963115428755Clontech Universal Primer Mix Long (100% over 8bp)
CTCTGAGA17300530.44010348260998744No Hit
GGATATGC17285810.43972902452899104No Hit
TCCGGCGA17080130.4344967868863742No Hit
AACAGTGA17019110.43294451568376585No Hit
ACCATAAG16993450.4322917579148552No Hit
AACGTCGA16980110.4319524052789523No Hit
CTGTAAGG16931140.4307066707527031No Hit
AGAAGAGC16913410.43025564209943784No Hit
TGGCGCGA16812270.4276827691162879No Hit
TGATTCGC16625280.4229259813061317No Hit
AGGATCTT16486480.4193950858141285No Hit
GAACGCGC16446180.41836990506248783No Hit
CGCCGGTA16427640.4178982710392764No Hit
TCATTGCG16360850.41619921837421237No Hit
CAATCAGC16338550.4156319347324856No Hit
TTATGACG16290760.414416217905664No Hit
CTACAAGC16237940.4130725442753499No Hit
AATATCAA16066800.4087189603092013No Hit
AATGGTCT16009410.40725903169042554No Hit
AGTCTACC15916770.40490238789806837No Hit
GAACAGAC15778260.4013788696372805No Hit
GTATCTAG15775050.4012972113193458No Hit
TGCTCAGC15718990.3998711162092471No Hit
TCAATAAC15576240.3962397377403461No Hit
CAGTACCG15533820.3951606268846488No Hit
CTTGCTTG15291910.3890067441146885No Hit
GAGTCCTA15289670.38894976136323256No Hit
ACCGAACT15278690.388670444126185No Hit
GTGAGTCA15262270.38825274020703016No Hit
CACACATG15248070.38789151026476476ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CTCGAACA15232320.38749085029359004No Hit
GCAGTTAA15228590.38739596383692454No Hit
CTAGCGGC15218040.38712758525305824No Hit
CATTCCAG15193380.38650026621247613No Hit
TGTCGGCT15121770.38467859887686845No Hit
ACACTGTG14865050.3781479685403622No Hit
ACGGACGG14849900.3777625718061846No Hit
GATCGATT14766790.37564835909479855RNA PCR Primer, Index 42 (100% over 8bp)
GTCTCCAC14754480.3753352083490741No Hit
GCGCAATC14688730.37366261196147166No Hit
CACATTAT14686920.373616567863197No Hit
CCACATAT14656290.3728373796144934No Hit
CCGAATAG14655120.372807616302349No Hit
AAGTGGAG14634400.3722805258513814No Hit
CGACCTCG14561050.37041459512848196No Hit
ATACCGAA14492910.36868120018017436No Hit
ATACTTAG14429270.3670622795093453No Hit
TGCTCGAG14307410.3639623160752277No Hit
TCGTCGAC14244810.3623698516818603No Hit
CGGAGCTG14175000.36059397405724397No Hit
CCGGCTGA14167850.3604120871496948No Hit
CAATACAT14146640.3598725317218462No Hit
GGCAACAC14117380.35912819453095274No Hit
CTCCAATG13880590.3531045580500346No Hit
GCCTACAT13784770.3506670191015926No Hit
GATCGGCG13697840.34845563044799144No Hit
TACGAGCG13696050.34841009512428334No Hit
AGCCACGC13592930.3457868534590429No Hit
TACATGTC13503050.3435004205568725No Hit
GTCCGACT13450070.3421526767300258No Hit
TAAGACTG13445290.3420310796086153No Hit
AGGTAGCC13247160.336990904364879No Hit
GATCTTCC13173110.33510716653214967No Hit
GTCCATCC13123770.3338520196764188No Hit
GTGTTAAG13107500.33343813156651325No Hit
GCTGCATG12949250.3294124528085197No Hit
GATACGAG12805880.325765302328055No Hit
TGAGTAAG12778000.32505607058225494No Hit
GGTCGTAT12775280.32498687724120123No Hit
GTAATTCC12770830.3248736749001392No Hit
TATACTAT12693820.32291463843155727No Hit
AACACGTA12672790.32237966197480783No Hit
AAGGCCTG12628300.3212478929514705No Hit
TCAAGGTT12588730.3202412824715096No Hit
GCACTCTC12576940.319941359864516No Hit
GTCATACA12559580.3194997435407323No Hit
CCGCGTAC12551200.31928656699733904No Hit
CGTTCATA12512580.31830412330929025No Hit
GACTAAGA12366150.31457913032014023No Hit
AGAAGTAG12185290.30997828191463805No Hit
CAAGAGAG12163150.3094150684694439No Hit
CATTGTGA12108420.30802280686802214No Hit
AGCTCACG12072900.30711922323779195No Hit
CGTATGAC12020970.3057981900756902No Hit
TCGTTCTG11976960.3046786316419505No Hit
TAGCGCTG11646510.2962724038657801No Hit
GGTTCAAT11629900.2958498665882428No Hit
CGTACACT11518960.2930276940674731No Hit
GGCGTGTC11323170.2880470454480256No Hit
ATGAATTG11238760.28589976238981246No Hit
ATGCGGCA11150870.2836639525570159No Hit
GTCGAAGC10983770.2794131410533146No Hit
ATTGCCAG10927360.27797814238830093No Hit
GCTGTAGC10775480.27411450832976025No Hit
GCGCCTAA10714670.2725675792601009No Hit
CAATCGTA10685670.27182985613857286No Hit
TTGCGTCG10489620.26684259344975997No Hit
GTAGAATG10312650.2623407016974559No Hit
CGATGGCA10296590.2619321557204993No Hit
GAAGGCAG10062660.25598127400260084No Hit
ATATTCGG9877130.2512616267358043No Hit
CAGGAATC9819450.24979432088581333No Hit
CACAGGCA9673980.24609374907585868No Hit
CATACGTT9632840.24504719979242204No Hit
GCGACAAG9578460.2436638417459153No Hit
GCGGAGTA9085510.23112382061635492No Hit
AAGCTCCA8506660.21639861272337177No Hit
GTGGTCGT8421580.21423428571717848No Hit
CAGATAGT8298790.21111066426571537No Hit
AGCGTGCG7993390.20334167663417518No Hit
TGCAATCG6739830.17145270435063384No Hit
GTTAATGG5784140.14714116607431868No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)