FastQCFastQC Report
Wed 29 Mar 2023
EGAF00001836356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405601862
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCGCT64875511.5994874796703966No Hit
GCGCTCAT58299111.4373481845603557No Hit
ATGTAATA53351791.3153733993459822No Hit
CGACTTAC50039841.2337182022108173No Hit
AGCGTTAT48982541.2076507676387345No Hit
TGCTATAC47456031.1700150922877173No Hit
CCTTGGTT47139211.1622039841621832No Hit
CACGATAC46954411.1576477920606785No Hit
CCTGTGCG45868331.1308707946710561No Hit
GAGAAGCT44707221.1022439537025597No Hit
CACACCAC43707001.0775838105003572No Hit
CTGATTAC41978461.03496714223664No Hit
AATGTAGG40605101.0011073371255874No Hit
CGTATCCA40540900.9995245041552594No Hit
TTCCTTAT39419200.9718693056690159No Hit
ACTGTCAT37954190.935749895546584No Hit
GTGCCACG37571570.9263165069000595No Hit
TTAGGTCA37301100.9196481450077761No Hit
ACACGAAT36817720.9077305468582884No Hit
CTTCTGCT36285650.8946125104327061Illumina Single End Adapter 1 (100% over 8bp)
TCTGTGTT35965550.8867205348283139No Hit
GCACGTCC35279690.8698108491425022No Hit
GGTTAATG34712940.855837787056313No Hit
AGCAACTA33791070.8331093411006086No Hit
GGTCTCGC33014000.8139508984798496No Hit
CCAAGACG31207850.7694207774618155No Hit
TCTCTGCC30129770.7428410178255049No Hit
GACATCTA28002470.6903930337479565No Hit
CTCGGCCT27936620.688769520491008No Hit
TATGCCGC27761600.6844544515429271No Hit
ACCTATAA27289080.6728046036435602No Hit
AGTGCTCA26905430.6633458206362968No Hit
AACGCGCC26848720.6619476515125071No Hit
TTCAGTAA25756420.6350173017696846No Hit
TTCCGAGC25522380.6292471112965453No Hit
CATGTATT25319440.6242436825894059No Hit
CTGCGCTT25299080.6237417125072271No Hit
GATGGTAC25159950.6203115014299416No Hit
ACTGGTTA24946280.6150435275861726No Hit
ACAACCTT24751850.6102499105391188No Hit
CCACCAGG24509810.6042824823126675No Hit
CTGTGTTG24304100.5992107600334439No Hit
TATACGGA24010050.5919610398632735No Hit
ATACGTTA23923580.5898291462971637No Hit
CCTATACC23160780.5710225265188748No Hit
CTATCGCT22970650.5663349247642261No Hit
CGATCTAA22888270.5643038689994969No Hit
CCTAACCT22832840.562937257916237No Hit
CGGTTGTT22531050.5554967102197376No Hit
TGAATTGA22346150.5509380526463165No Hit
GTGTAGAT22148580.5460670197810877Illumina Single End PCR Primer 1 (100% over 8bp)
CCGTTACT22095470.5447576076462883No Hit
ATTAGGCT22074720.5442460222236356No Hit
TACCTCGC21797260.5374053238443959No Hit
GCGGCTCC21794760.5373436870464859No Hit
AGTCTGAT21784610.5370934416469716No Hit
AACAGAAT21604090.5326427717434886No Hit
TCTCTCAG21363770.5267177496339994ABI Solid3 Adapter A (100% over 8bp)
CAAGGTCC21170960.5219640732319912No Hit
TCAACTTA21124400.520816149507716No Hit
GCTGACAA20963550.5168504379301888No Hit
AGCAGGAA20908920.5155035506222602Illumina Paired End Adapter 2 (100% over 8bp)
TGCGGATT20850730.5140688925141078No Hit
GCACACCA20676280.5097678767559505No Hit
CTTCGCAA20669330.5095965264577608No Hit
AGGTCTTG20612970.5082069864856784No Hit
TGTGTCGA20582690.5074604415893929No Hit
GTCGACCG20029120.4938123287017849No Hit
TTGATGAG19845820.4892931186790262No Hit
ACAGTGCC19376700.47772709682481684No Hit
CTGCCTTA19327600.4765165501138651No Hit
GTGTGTGT19304240.4759406158741944No Hit
ATTAGTGC18981360.46798010015052643No Hit
CATCAGTG18889410.46571309872339783No Hit
CACCACGG18864480.4650984565746397No Hit
CTCATAGG18777910.46296409753661344No Hit
TGGTTAGT18512620.4564234470895994No Hit
ACGATAGA18458960.45510047485925986No Hit
TGGTTCCA18425250.4542693642762419No Hit
TCAATCGG18297590.4511219428277674No Hit
CGCGGTCG18208590.4489276728221726No Hit
AATCAGCT18167570.4479163362420659No Hit
CACCGGAG18094440.4461133366296035No Hit
AAGCATCC17962250.4428542293033162No Hit
CGGCTTGA17506590.4316200599690541No Hit
CCTGGTGC17388960.42871992535379433No Hit
TCGGAGCT17388680.4287130220324284No Hit
ACCGACAC17373490.42833851684832747No Hit
CCAGGATA17247810.42523991174379766No Hit
ATACATGT17145650.4227211856340048No Hit
GTGATGTC17068770.42082573082467756No Hit
CTCAATTC17002410.41918964366095535No Hit
AAGATGGC16883220.41625104768379984No Hit
AATATTCG16873420.4160094314359927No Hit
CAGCACAA16707050.4119076258086803No Hit
ATGGAGAG16694190.4115905661202315No Hit
GGACGTTG16670050.41099540119961286No Hit
AGTAACCG16602430.4093282490897441No Hit
CATGACTA16588280.40897938481357365No Hit
CCATAAGA16547100.4079641034784007No Hit
ACGACGAT16245410.40052602125381764No Hit
CGGTCGGA16079450.3964343240613624No Hit
GCAAGCAC15994990.39435198648077213No Hit
GTAGTGAC15843040.3906057019038044RNA PCR Primer, Index 43 (100% over 8bp)
CACTTGCT15528890.382860421878438No Hit
CAACTCGA15490600.381916392681649No Hit
TCTCATTC15247960.3759341716236993No Hit
CTTCGACG15149640.3735101196354962No Hit
CTAGGCAC14961740.36887749790458313No Hit
AGAGATCG14837470.3658136559540745No Hit
TATGAGGT14801490.36492657915855425No Hit
TAGGTGAA14764510.36401484764387004No Hit
TTCTATGA14692430.3622377354865299No Hit
TCCAGATC14438850.3559857918009262No Hit
TAAGTCCT14397660.35497026391856157No Hit
GATTGAGG14153840.3489589502919984No Hit
GGCTACGG13827980.3409249635052218No Hit
TATCACAT13641250.3363211877957306No Hit
CGCCAGCC13375910.3297793046127584No Hit
CGACGCCT13342590.32895780937021435No Hit
GAGAATTC13227390.32611758572252314Illumina Small RNA Adapter 2 (100% over 8bp)
TTGCTCTA13212150.325741847802464No Hit
TGTAGCAC13034250.3213557732631908No Hit
ACAAGTGT13016190.3209105090350892No Hit
TTGACTCT12869390.31729119626181596No Hit
ACGATTCT12708220.3133175951741563No Hit
CGGTATAT12594090.31050375207597053No Hit
CATATCTC12593560.3104906850748136No Hit
TTGCAGAA12589110.3103809715745338No Hit
TCATCATG12495450.3080718105776349No Hit
GCGATCCA12228950.30150132792043244No Hit
CTATTGGA12166700.2999665716524743No Hit
GCTAGTCT12027800.29654203116059663No Hit
ACTTAAGG11814320.29127874171346874No Hit
AGACTCAA11690530.2882267340281589No Hit
TGCGCCTG11638130.286934826743966No Hit
GTCACAAT11606560.28615647725995896No Hit
TACCTGCA11378570.28053544783776163No Hit
GACAAGGC11291830.2783968974974774No Hit
AATCCAAG10976480.2706220318091143No Hit
TGCCGTAG10797920.2662196851551929No Hit
GACGCAAG10779400.2657630797562759No Hit
CACGCCGT10599730.26133336636408244No Hit
GTATATCA10386660.2560801853518118No Hit
TACAAGAA10237100.252392825553646No Hit
TATGCTTA10180130.2509882462028737No Hit
CGAGTTCT10097990.24896310757074386No Hit
ACGCCTCG10045840.24767736396634196No Hit
GTAAGAGA10043210.24761252205494064No Hit
AAGGTGTT10042810.24760266016727508No Hit
AGAATGTA9981270.24608540874992332No Hit
CTCTAGTT9763990.24072843136997238No Hit
AAGAACGT9560910.23572155100215No Hit
AACGGCCG9547240.23538452099117832No Hit
CCATGCGG9450250.23299325977946322No Hit
TGGCACAG9207770.2270149834765798No Hit
TTATACGT9140870.2253655827645091No Hit
GAGTTCCT9103080.22443388092730204RNA PCR Primer, Index 1 (100% over 8bp)
AACCGTTC8534290.21041052321401818No Hit
ACTATTAC8268840.20386592801193798No Hit
GTTCACTG8200370.20217781939077983No Hit
GTCCGCGG8085490.19934548525322107No Hit
GCTACCTC7926170.19541749539601475No Hit
CATTCTTC7498350.18486971344327802No Hit
TATTGACC7447370.18361281586029798No Hit
ATCTAACC7395500.18233397557726203No Hit
GCCACTGT7337140.18089512616685177No Hit
ATGAGACG7094520.17491339820328536No Hit
TTCGACAA6545860.16138633998677254No Hit
GCCAACTG6195620.15275127114677792No Hit
TGGCCGCA6145820.1515234661324114No Hit
TCTGATAT5963250.14702225405464237No Hit
AGGACCAG5950350.14670420817742696No Hit
TTAAGTGG5810600.14325871117425984No Hit
TCTTAGTA5807250.1431761178650605No Hit
AACAATAG5712120.14083071443099046No Hit
CCTTGTAG5396700.13305412291228585No Hit
GAGCGTTA5098120.12569271686430275No Hit
ATAGCCTA4911780.12109855649528553No Hit
TCCACAGG4896980.12073366665165852No Hit
TGAGATAA4581270.11294992526439634No Hit
ACCAGGCC4379630.10797854769217011No Hit
TGACGACC4124520.10168888228624551No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)