FastQCFastQC Report
Tue 28 Mar 2023
EGAF00001836360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395836453
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGCTT40781501.030261353923359Clontech Universal Primer Mix Long (100% over 8bp)
TCTAGTGA37011150.9350111572468036No Hit
CAGTGGTC34831230.8799399281197581No Hit
TACTGCAC34410170.8693027066913415No Hit
GCAGCTGG33882070.8559613381539674No Hit
GCCATGCG33785300.8535166416317902No Hit
CCAGCGTG33029040.8344112764167275No Hit
GACCACTT31760740.8023702657824695ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CCACTACA31152970.7870161973182395No Hit
CTTGCCGA30960040.7821422146787476No Hit
ATAGGTAT30900580.780640079149052No Hit
TGTGAACA30889810.7803679970828761No Hit
TACTGTTG30158660.7618969847630481No Hit
GCTGGAAT30122750.7609897919128736No Hit
TAGGCATT29984370.7574939036754152No Hit
ACTCTTGT29044260.7337439434866804No Hit
CGCTTACC28672860.7243612805918105No Hit
TGAGCGTA28620360.7230349752552981No Hit
ACACTAGC27986140.7070127015310538No Hit
ACAGGCTG27673790.6991218163527754No Hit
CCGGTAGG27658800.6987431246005026No Hit
CGCGTCGG27516340.695144163491178No Hit
ATTAATCA27445040.6933429145294003No Hit
TGTCTTAA27354810.6910634377577145No Hit
TTACAATT27306610.6898457631440023No Hit
TCGGATTG27066430.6837781057016495No Hit
TACTCAAT26742850.6756035174961513No Hit
GATATTGT25781000.6513043405833064No Hit
GCGTACGA25734780.6501366866279998No Hit
AATCTGTA25686820.6489250751243975No Hit
AATCGCGG25624270.6473448770520385No Hit
GACGCCTC25572480.6460365084162676No Hit
CTTATGTA25483030.6437767367524385No Hit
TACTCCGG25000790.6315939275052063No Hit
ACTCCGTT24871400.6283251532672762No Hit
ATAAGCCA24572980.6207861811049524No Hit
GGTAGCGA24459930.6179302036136626No Hit
GCATCCGT24294680.6137554996734977No Hit
ATAGTTGC24119750.6093362502922387No Hit
ACTCATAG24057090.6077532732944129No Hit
CCGAAGGT24043250.6074036339447494No Hit
CGAGGAGT23981710.6058489514607691No Hit
GCCTTAGG23785050.600880737984988No Hit
AGATGGAC23616890.5966325188347421RNA PCR Primer, Index 16 (100% over 8bp)
GGCTGAGT23329690.58937699707005No Hit
GTGTCGGC23185700.5857393836337756No Hit
TCGGCAGC23163240.5851719775793363No Hit
GACTCGGT22941150.5795613270615073No Hit
ATTCGATC22938750.5795006959604097No Hit
TCTCCACG22779700.5754826223647472No Hit
AGCGAAGA22738150.5744329464269932No Hit
CGATGCTC22732410.5742879370435345No Hit
GGACCGGC22710050.5737230572849742No Hit
TTAGAGAT22697510.5734062597817386No Hit
CTTGCGAT22625910.5715974319323238No Hit
ACCTAGGT21882920.5528273061804139No Hit
GAGGTATA21796760.5506506496510063No Hit
CGTGTGGT21709780.5484532774953903No Hit
AAGCAGGA21660270.5472025084056622No Hit
GGCACTCC21636880.5466116077995474No Hit
GGTTGGCC20996650.5304375036929709No Hit
ACAGCGGA20753990.5243071941128171No Hit
AGTGTCTC20531710.5186917436328179No Hit
GTTATCCG20404640.5154815794592824No Hit
GGCCGTCA20391240.5151430558114869No Hit
TGGAGTTA20048670.5064887240185532No Hit
GCGAGATA20026340.5059246021487566No Hit
GGCTCTTA19957710.5041908052869501No Hit
CGAGAGGA19892390.502540628818741No Hit
TTAAGGAC19649350.4964007193142467No Hit
CGGATGCG19611540.4954455268423699No Hit
GCATGTAT19247590.48625107299049086No Hit
GACTTATC19221060.48558084669377327No Hit
GTTGCCTT19128110.48323265467417675No Hit
TCGCAACA18597120.4698182761858974No Hit
GGTCCGTG18469860.46660331205019157No Hit
CGTGATCC18359520.46381579717722465No Hit
AGAGCCGC18329880.46306700307866794No Hit
GATTACGC18048670.4559628064371323No Hit
CAAGACGC17988730.454448544687217No Hit
CGCACCGA17856810.4511158551635465No Hit
GTTACCAA17679230.4466296589414922No Hit
CGCAAGTG17442070.44063829563468726No Hit
ACCTCGCA17314320.437410952649174No Hit
TGGCTGTG17244940.43565820856827453No Hit
AGTTACAT17147890.43320643841763606No Hit
TGTGGAGC17130350.43276332612044704No Hit
TTCACGGT17018810.42994549569693113No Hit
CTAATGAT16855710.42582510711816635No Hit
TAATAGGC16818610.4248878513470309No Hit
ATATACAA16442770.4153930209151303No Hit
ATCGCGTG16437510.4152601377518912No Hit
GATGGCGT16352150.4131036915895161No Hit
TTCGCATA16273510.41111701251021465No Hit
ACTACATA16179710.4087473469756461No Hit
CGGCACTC15987130.403882206371731No Hit
AAGTGAGA15959230.4031773698214702No Hit
ATCGCTAA15609340.39433811317018846No Hit
GATACACC15533190.39241433885827587No Hit
ATTCTGGC15457330.3904978908044126No Hit
TCGTGAAG15400150.38905335482075976No Hit
GCACCACT15356940.3879617423714132No Hit
ATGTGCAT15348640.3877520598134503No Hit
GGAGTGCA15272620.38583156968618043No Hit
TGCAGTGT15204170.38410232015695633No Hit
CCTACTCG15202750.3840664467554735No Hit
TAATTAGA15068620.38067792609287554No Hit
TCGATTGC15038690.3799218057362696No Hit
CATCTCCG15020240.379455704146581No Hit
CGCCTCAT14994090.37879507777420385No Hit
AGCAAGCT14985030.37856619536756003No Hit
CCGACACA14843530.3749914866986745No Hit
TAACGGTC14819890.37439427035286216No Hit
GAACAAGT14811450.3741810509806685No Hit
GGAATATC14773560.37322383747208854RNA PCR Primer, Index 37 (100% over 8bp)
GGCCTAAG14650840.3701235671692925No Hit
TGATCCAT14294050.36110999610235495No Hit
TCTATGAA14219520.35922714778368325No Hit
AATTAACA14161530.35776214880340995No Hit
AACGGATA14147860.357416804156741No Hit
GGAAGAAT13866250.350302502331689No Hit
ATAACTGA13860850.35016608235421914No Hit
TACACACA13779920.348121551099287No Hit
ATGGTCAA13686100.3457513803055425No Hit
GGACACAT13675560.3454851087198884No Hit
TGACGGAA13656100.3449934915418212No Hit
TTATGCTA13554180.342418690781872No Hit
CAGGCTAG13419290.339010970270593No Hit
TTAATACT13348120.3372130054934582No Hit
TATCAAGA13249010.33470919364771085No Hit
CAGGCGCA13103340.3310291384406681No Hit
TGTGAGTC12994490.3282792653762992No Hit
AGGTTATC12936450.32681300324808643No Hit
AGCTTCAC12862330.32494051274251895No Hit
CGTCGTGG12710820.3211129218561384No Hit
GTTCAGGA12548940.3170233540870982No Hit
GTGGTACC12532050.3165966627131231No Hit
CGCGATTA12509870.3160363302871451No Hit
GTGCGGTG12463530.3148656447767836No Hit
TAGGATCA12410440.3135244342945848No Hit
GGCGGAAC12290260.3104883319071172No Hit
ATTGACCT12159110.3071750948617155No Hit
CCATCTCC12130800.30645990049835053No Hit
GGCCGCTC12120550.3062009551707457No Hit
TTCCGGTT12105290.30581544241959946No Hit
CTTACAAG12093770.3055244131343305No Hit
CGTAATGT11997310.3030875481293786No Hit
TCCTTACA11930310.30139492989040095No Hit
CTGAACTA11783230.29767925391146327No Hit
TCACCGAG11749100.29681702912793634No Hit
GTCTTGTA11661510.294604246567458No Hit
CTTAACAC11577530.29248266328821415No Hit
GGTGTACG11428810.2887255560568597No Hit
CGAGCCTT11423620.28859444130073586No Hit
CTGACTGG11263640.28455287315339806No Hit
ATCATCTC11076940.2798362787471724Illumina PCR Primer Index 7 (100% over 8bp)
TCCAACCA10997200.27782181041320114No Hit
ATCGGCGC10949180.2766086831320712No Hit
TTACAGCG10906940.2755415757527516No Hit
TGTCCTGC10850730.27412154483912576No Hit
ACGTGGTA10838120.2738029789287749No Hit
TAACCTCT10797010.2727644186928888No Hit
ATAATATG10765420.27196636182469025No Hit
GTACTATA10730330.27107988460072424No Hit
GCGGAAGT10675170.26968637979382865No Hit
CTGGATGC10544120.266375669044306No Hit
GTTGCAAC10483030.26483235489178153No Hit
AGTGGATG10452000.2640484452805058No Hit
GACCTGTG10317040.2606389563621115No Hit
GCGCGAGG10259410.25918305204700287No Hit
GAGTATAA10243720.2587866762235766No Hit
TAGATCCG10201660.2577241161768393No Hit
TCATTCAA10181930.2572256779998986No Hit
AGGCCAGA10085280.2547840130327765No Hit
TCCGACTT10072360.2544576156052004No Hit
GAGTGAAC9762560.24663115097183835No Hit
TCACGCCG9699260.24503200568038638No Hit
CGGAGAGA9696070.24495141684184402No Hit
CTATCCTG9393620.23731063495559365No Hit
TTAACCAG9306670.23511402068874138No Hit
AACCAACG9171170.2316908897726001No Hit
ATCCTCCG9071880.22918253059427046No Hit
TCGCACGC8524880.21536369213575185No Hit
GGTAATTA8075520.20401152897355818No Hit
GTAGCTTC7756990.19596451870995318No Hit
TCTGACCG7326430.18508729917302488No Hit
TAATACCA6202600.15669602819526074No Hit
GGATTGTG6132590.1549273684503231No Hit
TTCTTGCC6011600.15187080306623504No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)