FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001836362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27100441
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATCCA2614170.9646226790183967No Hit
GAGAAGCT2576180.950604456953302No Hit
GATGTCCA2237750.8257245703123429No Hit
GCACGTCC1980790.7309069250939495No Hit
ATTATCGT1875340.691996119177544No Hit
TCTCTATA1795010.6623545351162367No Hit
GCTCCAGA1743930.6435061333503761No Hit
ACGTGCGC1594120.5882265901134229No Hit
CCAACAAT1545730.570370792121058RNA PCR Primer, Index 36 (100% over 8bp)
TGGCTTCC1533150.5657288012398027No Hit
TCCTCCTA1472810.5434634809079306No Hit
TCTGTGTT1471890.5431240030374413No Hit
CGCATATA1461830.5394118863231783No Hit
GCCGTCCT1453370.5362901659054182No Hit
CGACTTAC1449190.534747755580804No Hit
ATCCTAAC1434940.5294895385650735No Hit
TACAGCCT1428240.5270172540734669No Hit
GCATAACG1427230.5266445664112993No Hit
CACTGCTT1402290.5174417641395577Clontech Universal Primer Mix Long (100% over 8bp)
CCTGGCCA1385610.5112868827485132ABI Solid3 GAPDH Forward Primer (100% over 8bp)
AGGCCGAC1382740.5102278593916608No Hit
GTAGCACA1361580.5024198683704078No Hit
ACTTCTAT1296950.47857154796853674No Hit
ATACGTTA1287040.47491478090707084No Hit
TGAATTGA1281770.47297016310546386No Hit
CCTAACCT1272730.4696344240302215No Hit
GACGCTCA1269640.4684942211826No Hit
ACACTAGC1266650.4673909181035098No Hit
CGCTTACC1234420.45549812270582607No Hit
AGCGTTAT1230310.4539815422191838No Hit
GATGGTAC1214500.4481476888143628No Hit
TATAACGG1193250.4403064880014314No Hit
TAACTATG1170780.4320151100124164No Hit
TATTAGAG1159780.4279561354739577No Hit
GACCACTT1158380.42743953871451756ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
CAGTGGTC1135310.41892676211431396No Hit
ATGTAATA1132590.4179230884102587No Hit
TCGTGTCA1128640.41646554755326676No Hit
CTGATTAC1116910.4121372047045286No Hit
AGCAGGAA1103970.40736237465656006Illumina Paired End Adapter 2 (100% over 8bp)
GTATGCCT1091240.4026650341225075No Hit
TACGTCAG1083420.39977947222334875No Hit
CTTCGCAA1081090.39891970761656614No Hit
AATCTGTA1078400.39792710384307034No Hit
TGGAAGAT1077040.3974252669910427No Hit
CTCGTATC1069490.39463933446691885No Hit
GCGTCGCT1053230.3886394321037064No Hit
ATTAGGCT1046740.3862446371260158No Hit
TAGGCATT1046050.3859900287231488No Hit
ATAAGCCA1043230.3849494552505622No Hit
TTCGGAAG1037570.3828609283516825No Hit
CCTGTGCG1035060.3819347441615433No Hit
TACTCAAT1034490.3817244154809141No Hit
GCGCTCAT1026610.3788167137206365No Hit
AATCGCGG1014400.37431125198294746No Hit
CGGACATC1010410.3728389512185429No Hit
TTACAATT1002830.3700419487638596No Hit
TGTGTCGA998900.36859178786057395No Hit
GCCATGCG997770.3681748204761686No Hit
ACTGTCAT992930.36638887167924683No Hit
TACTGCAC990490.36548851732707965No Hit
AGAACACA987050.3642191652895981No Hit
CCGGTAGG977590.36072844718652364No Hit
TCGGATTG973300.3591454471165248No Hit
AGATGACT963130.35539274065687715No Hit
GATATTGT957460.3533005237811444No Hit
CCGCTGTA945890.34903122056205654No Hit
CACACCAC930070.3431936771803824No Hit
ATAGGTAT925670.341570087364999No Hit
ACGCATTA924840.341263819286188No Hit
CGCGTCGG921880.3401715861376573No Hit
GACGATGT919840.3394188308596159No Hit
TGAGCGTA918740.33901293340577004No Hit
TACTGTTG914910.33759967227101584No Hit
AGTTCGTC911550.33635984004835934No Hit
TTAGGATC901260.332562853866474No Hit
GGTAGCGA898530.3315554901855656No Hit
CCAAGACG889780.32832676043906445No Hit
TACTCCGG889330.32816071148067294No Hit
AGCGAAGA883530.3260205249058493No Hit
CGTTGTCT880080.32474748289151456No Hit
GATCTTCC875660.32311651312242484No Hit
GTCCATCC870450.3211940351819367No Hit
AAGTGGAG867630.32015346170935No Hit
CTTATGTA865330.3193047670331269No Hit
CTTGCCGA864450.3189800490700502No Hit
TCCGTATG861880.3180317250187921No Hit
CTTCTAGA859560.3171756503888627No Hit
GAACGCGC845260.31189898348886647No Hit
TGCCAGGT838290.30932706962222495No Hit
CGAGAGGA834200.307817869089289No Hit
GTGTCGGC829790.3061905892970524No Hit
TTGTGTAC829630.3061315496674021No Hit
TAGGTCTC829320.30601716038495463No Hit
CTCTCGAA811450.2994231717483859No Hit
GGCCGTCA808660.2983936682063587No Hit
AATATCAA804300.29678483829838787No Hit
AATGTAGG803690.29655974971034604No Hit
CTTGCGAT801490.2957479548026543No Hit
CCGGCTGA789630.2913716422548253No Hit
GCCTGGTC785020.28967056292552584No Hit
GCGTACGA780910.28815398243888357No Hit
GACTTATC753820.2781578351437159No Hit
GTTATCCG751910.2774530495647654No Hit
CGAGGAGT746190.2753423828047669No Hit
ATTCGATC743440.27432763917015224No Hit
CACTCTCG735940.2715601565302941No Hit
ACCATAAG735490.27139410757190263No Hit
CGATCTAA735160.2712723383357488No Hit
TGTATATT734060.270866440881903No Hit
ACTCGGAA732660.27034984412246277No Hit
GAGGTATA716300.26431304199071887No Hit
ACAGGCTG709480.26179647777687454No Hit
CGTTCGCG707240.26096992296177024No Hit
GAGTGGCA706120.2605566455542181No Hit
AATTATTG701290.2587743867341495No Hit
CGTGCCAC700800.25859357786834536No Hit
TGCTTCTT694140.25613605328415134No Hit
CAGCTAAC693840.256025353978557No Hit
CGATGCTC692500.2555308970802357No Hit
TATAGGTG684000.25239441675506313RNA PCR Primer, Index 39 (100% over 8bp)
CATGTATT681150.251342773351917No Hit
TTAGGTCA680320.2510365052731061No Hit
TCTGCGCA674540.24890369865198872No Hit
ACTCCGTT664920.24535394091926402No Hit
ACTGAGGC664330.2451362322849285No Hit
GGCTGAGT653650.2411953370057705No Hit
CCACCAGG650690.2401031038572398No Hit
GGCTCTTA649120.23952377749129616No Hit
ACTTGTCC644190.2377046189026961No Hit
ACCTAGGT641360.23666035545325628No Hit
TTGACCGA636680.23493344628598478No Hit
GTGCCACG631560.23304417813717498No Hit
CTTCCTGT629080.2321290638775952No Hit
GGAACCTG624470.23042798454829572No Hit
GTTACCAA595850.219867270794597No Hit
GGTTAATG588690.2172252473677458No Hit
TCGGCAGC584440.2156570072051595No Hit
GTATCTAG579920.21398913766753833No Hit
TGAGCTCC578560.2134873008155107No Hit
AAGTGAGA574270.21190430074551186No Hit
CGCAAGTG569290.21006669227264604No Hit
TTAAGGAC568350.2097198344484505No Hit
AGAGCCGC568210.20966817477250646No Hit
TAATGATT560730.2069080720863546No Hit
CAGAGTGC555970.20515164310425799No Hit
GCTGACAA555390.20493762444677563No Hit
AACTTGAA553520.20424759877523765No Hit
CGTGATCC548120.20225501127453976No Hit
AGTGCTCA545380.20124395761677827No Hit
CTTCTGCT544430.20089340981572956Illumina Single End Adapter 1 (100% over 8bp)
GTTAAGCC542120.20004102516265326No Hit
GGTCTCGC541160.1996867873847514No Hit
GGAGAACC531690.19619237930482386No Hit
AGCTGTGG521860.1925651320581831No Hit
GACTCGGT513260.1893917519644791No Hit
GCGCAATC512090.18896002467266124No Hit
TTATGACG511990.1889231249041298No Hit
GGAATATC510060.18821095937147295RNA PCR Primer, Index 37 (100% over 8bp)
TGGAGTTA504790.18626634156986596No Hit
TACACACA500860.18481618066658032No Hit
CATCTCCG496240.1831114113604277No Hit
CACACATG491330.18129963272553387ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
GGAAGAAT487550.17990482147504536No Hit
TGGCTGTG487420.1798568517759545No Hit
GAACAAGT482690.1781114927244173No Hit
CCGACACA479650.17698973976106144No Hit
GGTCAACT470690.17368352050064426No Hit
CGGAGCTG469630.17329238295421096No Hit
GCACACCA468000.17269091672714845No Hit
TATGCCGC463880.17117064626365305No Hit
ATTCTGGC460730.17000830355491264No Hit
AGCAAGCT457830.1689382102675008No Hit
TCTCTGCC455250.1679861962393896No Hit
CGCCTCAT454390.16766885823001923No Hit
CTATGGAG446690.16482757605309817No Hit
TAATTAGA446490.1647537765160353No Hit
CCTACTCG444910.1641707601732385No Hit
NNNNNNNN440400.16250658061247047No Hit
GGAGTGCA434290.16025200475519938No Hit
TTAATACT433170.15983872734764723No Hit
ATGTCAAC432600.159628398667018No Hit
TAACGGTC425960.15717825403653024No Hit
AGTTACAT425380.15696423537904788No Hit
CCGTTACT422960.156071260980587No Hit
CCGGTCTT422400.15586462227681092No Hit
TTGTGGCT418820.15454361056338528No Hit
TTCAGTAA418230.1543259019290498No Hit
TCGTGAAG414730.15303441003044932No Hit
TGTGGAGC412950.15237759415058966No Hit
CCATTGAC409870.15124108127982125No Hit
TTAGCAGT407040.1501968178303814No Hit
TTCCGAGC406580.1500270788951368No Hit
TAGCTGGT406080.14984258005247958No Hit
CAAGAGAG402790.14862857766779514No Hit
TTGATGAG402500.14852156833905397No Hit
TGCTATAC401880.14829278977415902No Hit
ATCTGTCT401500.14815257065373955No Hit
AACGGATA400750.14787582238975375No Hit
GTTGCCTT399840.1475400344961176No Hit
CACGATAC397440.146654440051363No Hit
ATCTCAGT395770.14603821391688793No Hit
CTGTGTTG394600.14560648662507006No Hit
TCGTCTGG390850.14422274530514095No Hit
ATAACTGA390600.14413049588381238No Hit
CATAATAA390280.14401241662451175No Hit
CAGGCGCA389100.14357699935584073No Hit
TCTATGAA388980.143532719633603No Hit
TCAGGTAC388520.14336298069835837No Hit
ACGATAGA385980.14242572657765976No Hit
TGACGGAA380130.1402670901185704No Hit
GGCGTGTC379160.1399091623638154No Hit
CTATCGCT374820.13830771240955084No Hit
TGTACGCC371550.13710108997857268No Hit
TTCACGGT369130.13620811558011175No Hit
AAGCCTGT367770.13570627872808416No Hit
AGGTCTTG366060.1350752926861965No Hit
CAGGCTAG364770.1345992856721409No Hit
TCGATTGC363820.1342487378710922No Hit
CAGCGTCT362360.13371000125053314No Hit
GGACACAT360300.13294986601878547No Hit
CATCAGTG356820.13166575407389128No Hit
ATGGCGCT356350.1314923251617935No Hit
CTCACCTT353590.13047389155032568No Hit
TGCAGTGT350920.1294886677305362No Hit
TGGTTAGT348220.12849237398018726No Hit
CCTTGGTT348010.12841488446627122No Hit
AACAGAAT346880.12799791708186595No Hit
AACCTTCT344370.12707173289172674No Hit
GTCGACCG342090.12623041816920988No Hit
TTCGTTCG338290.12482822696501508No Hit
CTGCCTTA338180.12478763721963047No Hit
TCTAGTGA335920.12395370245081991No Hit
ATACCGAA335080.12364374439515577No Hit
TGAGGCGG334750.12352197515900204No Hit
TTATGCTA334310.12335961617746369No Hit
AATATTCG332130.12255520122347824No Hit
CTATCCTG331950.12248878164012164No Hit
CAAGGTCC330150.1218245858065557No Hit
CTGGCCTC329830.12170650654725508No Hit
CATCCTCA328780.12131905897767495No Hit
TGTCCTGC328420.12118621981096174No Hit
TAGCCACC328170.12109397038963315No Hit
AGGCGGTT326990.12065855312096213No Hit
ACTGGTTA325800.12021944587543797No Hit
CTCTTAAT324580.11976926869935439No Hit
ATTAATCA322840.11912721272690728No Hit
GTGTAGAT322050.11883570455550889Illumina Single End PCR Primer 1 (100% over 8bp)
ATGTGCAT321550.11865120571285168No Hit
GTGGTACC321240.11853681643040422No Hit
AAGCATCC318590.11755897256432102No Hit
ATCATGGA317970.11733019399942607No Hit
ATTGAGTA317440.11713462522620942No Hit
TGTGAACA316330.11672503779551041No Hit
TCATTGCG315240.11632283031851769No Hit
ACAACCTT313940.11584313332760895No Hit
GCTCACGT313890.11582468344334322No Hit
ATCCTCCG312730.11539664612837851No Hit
TCACGCCG311350.11488742932264459No Hit
CTCTATAG309710.11428227311872895No Hit
AGCAACTA308620.11388006564173625No Hit
TACCTCGC307100.11331918916005833No Hit
ACGCTATT304830.11248156441439458No Hit
CTCATAGG303780.11209411684481443No Hit
CTCAATTC302720.11170297929838115No Hit
TGGTTCCA301680.11131922170565416No Hit
CGCTGAAG301660.11131184175194789No Hit
CTTAACAC300140.11075096527026997No Hit
TGTTGCGT299010.11033399788586465No Hit
TCCGACTT295780.1091421353622991No Hit
TTCCTTAT293970.10847424955188No Hit
AGTAACCG293940.10846317962132056No Hit
CACCACGG292640.1079834826304118No Hit
GTTCTAAT292350.10787647330167062No Hit
CTCCTTCA291530.10757389519971279No Hit
AGCATAGT290380.10714954786160123No Hit
ACACGAAT290200.10708312827824462No Hit
GGTAATTA287350.10603148487509852No Hit
TTATCGTC286410.10568462705090297No Hit
CGAAGCAA285730.10543370862488917No Hit
GCAGTTAA283600.10464774355516947No Hit
GATTGGAT282410.10420863630964529No Hit
GGAAGGCG282380.10419756637908585No Hit
CGGTTGTT279540.1031496129527929No Hit
TCGGTAAT279370.10308688334628945No Hit
GACATCTA279070.10297618404069514No Hit
GCCTACAT276570.10205368982740907No Hit
TGTCTTAA276390.10198727024405248No Hit
CACATTAT273950.10108691589188532No Hit
AAGCGCAC273690.10099097649370356No Hit
GATTATCT273610.10096145667887839No Hit
AGTCATGA273100.10077326785936805No Hit
ACGGACGG272780.10065518860006743No Hit
AGTGGATG271420.10015335174803981No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)