FastQCFastQC Report
Fri 2 Jun 2023
EGAF00001836364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397542268
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCA52971061.3324635960471No Hit
ACGTGCGC48551621.2212945366604389No Hit
TCTCTATA45194211.1368403724053815No Hit
ATTATCGT42371381.0658333317150568No Hit
CGTTCGCG38400470.9659468461854224No Hit
GCCGTCCT37809940.951092325105918No Hit
GACGCTCA35316460.8883699380615296No Hit
GCTCCAGA35197700.885382582764759No Hit
TGGCTTCC34683950.8724594286411829No Hit
ATGCTAGG33714080.8480627775660826No Hit
GATTGGAT33375510.8395461988962643No Hit
TATCTACT33151990.8339236521133898No Hit
CCAACAAT33121220.8331496463666601RNA PCR Primer, Index 36 (100% over 8bp)
GTGGCGAA32449600.8162553421866577No Hit
AGAGTATT32358730.8139695475098512No Hit
GGCCTGCT32259810.8114812586419112No Hit
TAATGATT32168130.8091750887732018No Hit
CACTCTCG31762510.7989718969958686No Hit
AGCTGTGG30964900.7789083700654442No Hit
CGAAGCAA30756760.7736727003831452No Hit
CTTCCTGT29568120.7437729866752181No Hit
ATCCTAAC29221180.7350458643557368No Hit
AGGCCGAC29010560.729747811369834No Hit
GGTGAGAT28886190.7266193390032176No Hit
CGCATATA28836820.725377458479459No Hit
TCGCGGAT28350710.7131495763363709No Hit
TATTAGAG28107410.7070294723981401No Hit
GGAGAACC27804920.6994204701775258No Hit
ACTTCTAT26717250.6720606121812436No Hit
TGTACCTA26393560.6639183333330483No Hit
GAGACCAT26377250.6635080624936215No Hit
CTCACCTT25572150.6432561279244903No Hit
TAGCTGGT25438090.6398839078917767No Hit
TGAGGCGG25122880.6319549396946138No Hit
CCGGTCTT24779280.6233118335985345No Hit
CTGATCCT24260410.6102598881384859No Hit
TCTACCGT23863100.6002657307373414No Hit
GGTCAACT23456050.5900265679422043No Hit
CTCTCTGC23401240.5886478466234438No Hit
TCAGGTAC23024000.579158541199448No Hit
CTCTTAAT22862980.5751081542856218No Hit
TATAACGG22387680.5631521929134841No Hit
GCCACGAC22229810.5591810428570579No Hit
ATTGAGTA22090660.555680786124609No Hit
ATCTGTCT22079880.5554096199903956No Hit
TAAGCAAC22063370.5549943182393878No Hit
GTATGCCT21829940.5491224897876772No Hit
GGATCTCT21676590.5452650383329805No Hit
ATATGGTT21421860.5388574177978982No Hit
GGATCAGA21339920.5367962533231813No Hit
ATATTACA21244050.5343846858568508No Hit
CTATGGAG21232510.5340944022586298No Hit
TCGGTAAT21144610.5318833166188004No Hit
TGCCTGAC20941700.5267792052743433No Hit
CTGGCCTC20928140.5264381094691547No Hit
CTGTAGCA20846590.5243867552720206No Hit
TTCGGAAG20764900.5223318794367797No Hit
AATTGCTA20669130.5199228274262399No Hit
AAGCGCAC20606620.5183504160116126No Hit
CAAGCACT20517590.5161109057213509No Hit
CGCGCGCT20507920.5158676611464117No Hit
CAATTAAG20481570.515204838545621No Hit
TGCCAGGT20464540.5147764564245028No Hit
GCCTCATT20436800.5140786689882244No Hit
CAACGTAA20359610.5121369886635552No Hit
TTGTCACA20351670.5119372614737913No Hit
CTTAGATT20287700.510328124404623No Hit
GGAAGGCG20190480.5078825982851212No Hit
GAGTGGCA19998810.5030612241715138No Hit
CTGCTCAG19859560.49955845198327437No Hit
TGTAGACG19853100.4993959535392095No Hit
TGTACGCC19830460.4988264543482455No Hit
GGTGCGGA19560960.4920473009929097No Hit
AGGCGTGC19519800.49101193938954935No Hit
CTGTTCGC19487300.49019441625764437No Hit
TACGTCAG19380290.4875026270162548No Hit
AGTTCGTC19339290.48647129014215923No Hit
AACTACCT19235360.4838569769391163No Hit
TAATTGAT19045550.47908239030321176No Hit
ACATTCCT18946290.47658554888558413TruSeq Adapter, Index 27 (100% over 8bp)
GGATACTA18650100.46913502037977006No Hit
CAACCGGT18359240.46181856566758833No Hit
CCTTAGCC17902720.4503350068929023No Hit
ATAGGAGG17884850.449885494943144No Hit
CATAATAA17831740.4485495363728216No Hit
TGGCCTAT17822770.4483238999884159No Hit
TCGTCTGG17762620.4468108533304438No Hit
CATCGAGC17694150.4450885207507042No Hit
TTCGTTCG17509510.4404439831791672No Hit
TAGAGTAG17483010.43977738739469086No Hit
CAGCGTCT17479760.43969563508150034No Hit
CTAACATA17417280.4381239783036102No Hit
CTCCTTCA17370960.43695881918146123No Hit
AGCATAGT17365590.4368237392055126No Hit
ACGCGACC16955240.4265015663692898No Hit
ACTAAGTG16906530.42527628785374844No Hit
TTGGAACG16796740.4225145689413836No Hit
ACGAGTTC16740580.42110188896945167No Hit
ACTCGGAA16737100.4210143511079431No Hit
ATGTTGTG16662010.41912549535487387No Hit
AGGTGTAA16590430.4173249321000503No Hit
GCCTGTGA16460860.41406565603232914No Hit
TCCTGGAA16323470.41060967132179266No Hit
CGCTGAAG16253180.4088415574466662No Hit
TCTGCGCA16220920.4080300714086583No Hit
GTCCTCAA16187900.4071994679066428No Hit
AGGAATAC16155650.406388233414214No Hit
TTCACTTG16075190.40436429768519605No Hit
GGCGCCAT16009530.40271264941316887No Hit
CCAGCCAA15720900.39545228936511473No Hit
TATAATCC15601550.3924500928791803No Hit
CTCAGAAC15461150.3889183929493505No Hit
GCTCACGT15341400.38590613464025414No Hit
CTCACGCC15307490.3850531435817034No Hit
ATCATGGA15025030.3779479871559217No Hit
ACCTGAAC14854150.3736495763011545No Hit
GCAACAGC14834420.3731532768736934No Hit
CCAGATTC14832140.37309592448167045No Hit
GAAGCGAT14756450.37119197599385834No Hit
CGCGTGAA14648120.3684669827360345No Hit
GAGCCGTC14548620.3659641042245098No Hit
AACGAGAT14537250.3656780969011325No Hit
GTCAGATG14525990.36539485657912485No Hit
GTTGGTCG14468700.36395375195676044No Hit
GATTATCT14462260.36379175660385377No Hit
TCGAGCAA14456410.3636446024401109No Hit
AGCGCTTC14358490.3611814681300757No Hit
CTCTATAG14095420.3545640585820675No Hit
CGAACGAG14054990.35354705980597767No Hit
AGGATTGG14037710.3531123890453832No Hit
GTTGTGTG13980430.35167153596859796No Hit
ATGCAACT13695860.34451330342563724No Hit
TTGAGAGT13559570.341084988728796No Hit
CGTGCCAC13500100.3395890471701993No Hit
GTCTGGCG13459320.3385632442988427No Hit
GAGAGCGG13390790.33683940244562877No Hit
AACCTTCT13338090.3355137572440473No Hit
CCGACCGC13187670.3317300086440117No Hit
GTCCAGAG13178080.33148877643370495No Hit
CATTAAGT12879110.3239683182569155No Hit
CCGTGGCG12717680.31990761797434836No Hit
CATCCTCA12580900.3164669775441338No Hit
ACCAGCGG12575880.3163407016634518No Hit
GGTTGCAG12534670.3153040823321962No Hit
AACTCTAC12407270.31209939165512834No Hit
CCGTACAC12359360.31089423678591077No Hit
TGATAAGG12273110.30872465616662426No Hit
ACATCCAG12222350.3074478108073781No Hit
ATAACGCG12140420.3053868978782402No Hit
AGTTCCGG12112660.3046886073508038No Hit
GTCATTAG12101100.3043978206614246No Hit
AAGTTACG12099390.3043548063674074No Hit
CGACAGTT12053480.3031999606139994No Hit
AACGCTGG12038970.3028349679788012No Hit
AAGACACT12022680.3024252002305325No Hit
TAACATTA11984690.3014695785757302No Hit
GAGTAATT11887230.29901801536233125No Hit
GATATATG11729090.29504007357527073No Hit
CGTCCGAA11705790.29445397237608956No Hit
AGCACAAC11700770.29432769649540763No Hit
TGCATCAA11427950.28746503000782797No Hit
CACGGCAA11286680.283911445612621No Hit
AACTAGTC11233640.2825772478613519No Hit
TAGTCGTG11188920.28145233603185055No Hit
TCGGCCAG11073230.2785422052278476No Hit
TTGTACTC11065560.27834926976871804No Hit
ACAGCACG11018580.2771675086383519No Hit
CTACGGCC10910670.27445308029484805No Hit
CAGATTCA10896120.2740870814773336No Hit
TATATAGC10763770.2707578757386372No Hit
AGATGCGA10738900.2701322818835455No Hit
GTTCGGAC10736900.270081972767736No Hit
CCAATCTA10410620.2618745436145673No Hit
CGCTACAA10065480.2531926994993146No Hit
CCTTACTG10034860.2524224669362705No Hit
GAGGTGCG9994440.2514057197057597No Hit
TGCTGCCG9505460.23910564398148476No Hit
GGCACGTT9500030.23896905473206184No Hit
ACCGTTCA9434890.23733048683014507No Hit
TGCCTAGA9294710.23380432090305425No Hit
GGCCAATA8817190.221792516412368No Hit
CGGCCAAG8712530.2191598403820547No Hit
CGAACTGC8648690.2175539734054141No Hit
CAATATGG8443840.21240106221862173No Hit
GCGCTGGC8130220.20451208976852747No Hit
ACCACCAA8046660.2024101749100048No Hit
AGACAGAG5765240.1450220634149021No Hit
AGCCATTG5694050.14323131043766144No Hit
CCCCCCCC4042870.10169660751646163No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)