FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001836368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401372724
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAAGAT43716801.0891821338611938No Hit
ACCGAACT40426951.0072171720368324No Hit
ACATACTC38914180.9695272666311027No Hit
CCTGGCCA35625700.8875964376692423ABI Solid3 GAPDH Forward Primer (100% over 8bp)
ATGCGGCA34722350.8650899257419395No Hit
CAGGAATC33603050.8372031279335266No Hit
TAACTATG32885400.8193232383175095No Hit
CTACAAGC31584860.7869209368596756No Hit
GTAGAATG30952290.771160772748474No Hit
CTCGTATC30516660.7603072699080569No Hit
GTCCATCC30238810.7533847765898511No Hit
TTGCGTCG30109270.7501573524961302No Hit
GCACTCTC29965050.7465641835691854No Hit
GCAGTTAA29848800.7436678731562237No Hit
GCGCCTAA28963480.7216105696310345No Hit
ACTGAGGC28933410.7208613906708817No Hit
TCCGTATG28758110.7164938791406265No Hit
GAAGGTGA28642400.7136110225566797No Hit
TCAAGGTT28343880.7061735465611759No Hit
ACGCTATT28328510.7057906107242106No Hit
TAGGTCTC27745250.6912589805180682No Hit
CCGCGTAC27730520.6908919899599356No Hit
TTATCGTC27600660.6876565932267984No Hit
GTTAATGG27128870.6759021821323364No Hit
TGAGCTCC27062510.674248856033376No Hit
TGTATATT26927290.6708799175900154No Hit
GACGATGT26427850.6584366206209867No Hit
CCGAATAG26300140.655254790058928No Hit
CTGTCATT25905210.6454153072942744No Hit
GCCTACAT25790560.6425588600783944No Hit
GTAATCTT25358680.6317987866061372No Hit
ATTGCCAG24915660.6207611656241993No Hit
TCCGGTCT24689010.6151142946126055No Hit
AACGTCGA24331240.6062006346001728No Hit
TTAGCAGT23961090.596978533100321No Hit
TGCTCAGC23464300.5846012595514587No Hit
CAGAGTGC23036480.5739423389417961No Hit
TCCGGCGA22710020.5658087518672544No Hit
GTGGTCGT22645720.5642067496345368No Hit
TTATGACG22584560.5626829789260916No Hit
GTCTCCAC22436290.558988906281534No Hit
ATGGCGCT22416820.558503821002047No Hit
CTTCTAGA22209690.5533432809948491No Hit
TACAGCCT22193920.5529503793586134No Hit
TGTTGCGT22083700.550204303369653No Hit
GGCGTGTC21951720.5469160879003826No Hit
TAAGACTG21690080.5403974585976101No Hit
GCCTGGTC21619220.5386320172568577No Hit
ACCATAAG21525660.5363010168075099No Hit
GTTAAGCC21471340.5349476612665888No Hit
ATCTCAGT21431610.5339578082540556No Hit
GTAGCACA21324390.5312864757596234No Hit
TCGTGTCA21281130.5302086745685289No Hit
TAGCCACC21215050.5285623245290579No Hit
TAGCATGG21071970.5249975581300337No Hit
TTGTGGCT20953880.5220554050404282No Hit
TCCTCCTA20876930.5201382344057839No Hit
GAGTCCTA20827970.5189184205750862No Hit
CTAGTCCG20764830.5173453191602526No Hit
AGAAGTAG20522400.5113052973674415No Hit
TGCTTCTT20448240.5094576381827082No Hit
ACTTGTCC20413700.5085970914157086No Hit
TGATTCGC20395270.5081379172143247No Hit
AATGCGAA20293390.5055996281401524No Hit
TTAGGATC20267200.5049471174329225No Hit
GGAACCTG20045350.49941983601257367No Hit
TCTCGAGT19708680.49103187191165487No Hit
CTCGAACA19637410.4892562156266503No Hit
TCAATAAC19406900.4835131746520972No Hit
CATTGTGA19294620.4807157747968943No Hit
GGCAACAC18969410.47261333084507257No Hit
CAGATAGT18853010.4697132832573845No Hit
ATACCGAA18832720.4692077680893932No Hit
CTTGCTTG18798580.46835718712166396No Hit
CAAGAGAG18659520.4648925770053074No Hit
ATGAATTG18563780.4625072629499358No Hit
CCATTGAC18432500.4592364876293886No Hit
CGTTCATA18398790.45839661989587516No Hit
CTCTGAGA18186120.4530980535687821No Hit
TGCTAACT18114000.45130121996032796No Hit
ATGTCAAC18090640.4507192172829362No Hit
TTACCTAC18014000.4488097701427265No Hit
CGGACATC17936020.44686693757496093No Hit
GTATCTAG17885290.4456030250824917No Hit
GTTCTAAT17873430.44530753913412413No Hit
GCTTCTCA17829690.4442177789839052No Hit
GCGGAGTA17821810.4440214527382783No Hit
ATACTTAG17800780.44349750084163664No Hit
TGCAATCG17799010.4434534021798651No Hit
TGTCGGCT17493540.43584277042203795No Hit
CGACTCTG17492320.43581237473426326No Hit
AGAAGAGC17342060.43206872223833526No Hit
AAGCCTGT17101120.4260658230478063No Hit
AAGGCCTG17090450.42579998535226826No Hit
AACAGTGA17053750.4248856232692085No Hit
AGCTCACG17047080.4247194435663745No Hit
CCGCTGTA16946530.42221429077477624No Hit
GCCGGTTG16762780.4176362517349336No Hit
GTGTTAAG16760220.4175724706196029No Hit
TACATGTC16657960.4150247140361237No Hit
AATATCAA16513400.411423074179799No Hit
TACGAGCG16171430.4029030632385473No Hit
AGTCATGA16115610.4015123359503622No Hit
AGGATCTT16066110.4002790682906494No Hit
GTCATACA15916530.39655235765348124No Hit
TTGACCGA15896030.3960416104408729No Hit
CTGTAAGG15434580.384544815257551No Hit
CGTTGTCT15408760.3839015229146463No Hit
GGATATGC15396150.38358735109264674No Hit
GAACGCGC15374720.38305343339673475No Hit
CATTCCAG15282160.3807473474455628No Hit
ACACTGTG15247310.3798790771841287No Hit
GCTGCATG15213880.37904618551010455No Hit
CAGCTAAC15190630.37846692342751226No Hit
TCCGCGAT15113790.3765524933876673No Hit
GCAGTGGT15057500.37515005628533943Clontech Universal Primer Mix Long (100% over 8bp)
CATACGTT15030100.3744673990353166No Hit
GGTTCAAT14985360.37335272438692174No Hit
GCAGATCT14910560.3714891199233558No Hit
AGAACACA14806250.3688902886186157No Hit
ACGGACGG14720920.3667643344892564No Hit
AGGCGGTT14706760.366411545195084No Hit
CGCCGGTA14669860.3654922002123891No Hit
AAGTGGAG14666780.365415463558007No Hit
CGAATCGT14608170.36395522481991077No Hit
AACACGTA14577540.36319209374077943No Hit
TGCTCGAG14469490.3605000822128611No Hit
GCGACAAG14292020.3560785062215638No Hit
TCGTTCTG14237390.35471742718620813No Hit
GCCATCGC14103300.3513766421257863No Hit
GTAATTCC14076900.3507188993739395No Hit
AGTCTACC13776120.34322511661255783No Hit
TATAGGTG13772300.3431299432295255RNA PCR Primer, Index 39 (100% over 8bp)
GAACAGAC13719970.34182616753997463No Hit
CAATACAT13584610.3384537410668693No Hit
CACAGGCA13427030.3345277144442929No Hit
CGTGTTAG13413580.33419261444382553No Hit
CTAGCGGC13349330.3325918579360166No Hit
GTGAGTCA13273220.3306956154798401No Hit
GATCGGCG13147080.32755290067991766No Hit
GTCGAAGC13135250.3272581621664954No Hit
GAAGGCAG13103790.326474352053878No Hit
GTCCGACT13076480.32579393710869103No Hit
CAATCGTA12903170.3214760054298059No Hit
CGATGGCA12754960.31778342765513884No Hit
GCTGTAGC12737980.31736037947611007No Hit
GCGCAATC12603910.3140200927056518No Hit
CGTATGAC12437660.3098780573838894No Hit
CGTACACT12430290.30969443753233217No Hit
CAACAGCA12343150.3075233881612743No Hit
TCATTGCG12305800.3065928316544001No Hit
AATTCATT12264360.30556037484998605No Hit
GATCGATT12233910.30480172838052644RNA PCR Primer, Index 42 (100% over 8bp)
GACAGTCG12175040.30333501187290446No Hit
AATGGTCT12097170.30139491989993816No Hit
CCGGCTGA12081780.30101148577300935No Hit
AAGCTCCA11945780.29762311402107133No Hit
GATCTTCC11921410.29701594770052187No Hit
TGAGTAAG11468530.28573266976656836No Hit
AGCGTGCG11352220.28283486448371614No Hit
AGACCATC11349790.2827743222531484No Hit
CAATCAGC11332980.2823555095388096No Hit
CGGAGCTG11324460.28214323801435No Hit
GCATAACG11233870.2798862336245848No Hit
GAGACTGA11126080.27720069986619217TruSeq Adapter, Index 15 (100% over 8bp)
GGTCGTAT11101680.27659278611069743No Hit
CAGTACCG11067000.2757287513139533No Hit
AGATGACT11005440.27419501480623776No Hit
AGGTAGCC10957630.27300385264844257No Hit
CTCCAATG10675400.265972233828226No Hit
GAGCTTAG10652350.26539795464526883No Hit
CCACATAT10285570.2562598150042702No Hit
AATTATTG10122920.25220747187594145No Hit
ATATTCGG10044390.250250936334179No Hit
AGCCACGC9890850.24642556428423373No Hit
TTGTGTAC9725760.2423124297803555No Hit
GACTAAGA9423470.23478102612672805No Hit
TACGTACC9366480.23336114887567697No Hit
GATACGAG9097830.22666786894069063No Hit
CTCTCGAA8616270.2146700431990491No Hit
AGAACGGT8438490.2102407437133172No Hit
CACATTAT8384500.20889560995679418No Hit
TGGCGCGA8221590.20483678905893962No Hit
AACTTGAA7764830.19345684287206324No Hit
TAGCGCTG7367720.18356304650138608No Hit
ACGCATTA7158340.17834644887329215No Hit
TATACTAT6805140.1695466481175238No Hit
CACACATG5984230.14909408741985167ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CGACCTCG4904660.12219714262397163No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)