FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21196642
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATACTC3873301.827317742121606No Hit
ACCGAACT3252881.5346204365766993No Hit
TGGAAGAT3033841.4312833136493979No Hit
CAGGAATC2598441.225873419006652No Hit
CTACAAGC2570211.212555271726531No Hit
GAAGGTGA2445991.1539516495112763No Hit
ATGCGGCA2340921.1043824771867166No Hit
GTAGAATG2211961.0435426517086999No Hit
TAACTATG2184381.0305311567747382No Hit
TTATCGTC2179041.028011889807829No Hit
GCGCCTAA2090730.9863496302857784No Hit
TCAAGGTT2072200.9776076795560353No Hit
CCGCGTAC2067720.975494137231737No Hit
CCTGGCCA2060540.9721068082387766ABI Solid3 GAPDH Forward Primer (100% over 8bp)
ATTGCCAG2024240.9549814541378772No Hit
GTCCATCC2014140.9502165484514009No Hit
TAAGACTG2011250.9488531249430924No Hit
CCGAATAG1994270.9408424221157294No Hit
ACGCTATT1992220.9398752877932268No Hit
GCAGTTAA1866210.8804271921939333No Hit
GACGATGT1837290.8667835216540432No Hit
TCCGTATG1813700.8556544003526597No Hit
CTCGTATC1796210.8474030933767717No Hit
TGTATATT1792380.8455962033986327No Hit
GCACTCTC1767000.8336226087132104No Hit
CTTCTAGA1734490.8182852736768399No Hit
ACCATAAG1732700.8174408002928011No Hit
GTTAATGG1731380.8168180601436774No Hit
TAGCATGG1729320.8158462080927724No Hit
TTGCGTCG1723570.8131335142613627No Hit
CTGTCATT1670360.7880304814319175No Hit
AACGTCGA1621210.7648428463338675No Hit
CTCGAACA1615790.7622858375397386No Hit
ATACTTAG1607780.7585069370893748No Hit
TTACCTAC1552890.7326113258883176No Hit
AATATCAA1546020.7293702464758334No Hit
GCCTACAT1540180.7266150930888015No Hit
TTAGCAGT1527530.7206471666597002No Hit
GTAGCACA1514590.7145424261069276No Hit
ATGTCAAC1508160.7115089267441512No Hit
GTTCTAAT1496390.7059561604144657No Hit
AATGCGAA1479000.6977520307226022No Hit
TCGTGTCA1466470.6918407170343303No Hit
GTGTTAAG1444000.6812399813140213No Hit
GTAATCTT1433610.676338261503874No Hit
TGCTCAGC1419020.6694550957646971No Hit
TCAATAAC1414870.6674972384776796No Hit
GGCAACAC1404110.6624209627166416No Hit
GGCGTGTC1396320.6587458522911318No Hit
ATCTCAGT1377730.6499755951909741No Hit
GTTAAGCC1364740.6438472659961894No Hit
CTAGTCCG1364710.6438331128109821No Hit
ACTTGTCC1358780.6410354998683282No Hit
AGAACACA1332080.6284391650337822No Hit
ATGAATTG1309240.6176638733625825No Hit
TGCTAACT1308120.6171354877815081No Hit
AGTCATGA1304090.6152342432353199No Hit
TAGCCACC1303740.6150691227412343No Hit
TACAGCCT1302760.6146067853577939No Hit
ATACCGAA1302570.6145171485181473No Hit
GTAATTCC1268060.5982362678012866No Hit
CCATTGAC1246550.5880884340076131No Hit
TAGGTCTC1233000.5816959120222911No Hit
AATTCATT1217220.5742513366032223No Hit
CATTGTGA1215130.5732653313671099No Hit
CGAATCGT1212170.5718688837599842No Hit
TCCTCCTA1203120.5675993395557655No Hit
TGAGCTCC1198760.5655424099722965No Hit
ATGGCGCT1188370.5606406901621492No Hit
GCTTCTCA1175460.5545501027945842No Hit
CAGATAGT1171670.552762083730055No Hit
CGGACATC1167760.5509174519246963No Hit
CATTCCAG1163570.5489407237240691No Hit
ACTGAGGC1158700.5466431899920752No Hit
TTGTGGCT1129510.5328721407853187No Hit
TCCGGCGA1126880.5316313782154739No Hit
GTGGTCGT1103700.520695683778591No Hit
TGTTGCGT1102080.5199314117773938No Hit
AAGCCTGT1075050.5071793919055669No Hit
AGTCTACC1052650.4966116802840752No Hit
TTATGACG1048260.49454059751539886No Hit
GTCATACA1044730.4928752393893334No Hit
CTCTGAGA1043280.492191168770978No Hit
AACACGTA1036830.4891482339513966No Hit
TGCTTCTT1015740.47919854475062607No Hit
AACAGTGA1004870.4740703739771611No Hit
GGAACCTG992630.46829587441256026No Hit
TACGAGCG991560.4677910774734979No Hit
GGTTCAAT952580.44940137216074133No Hit
GTATCTAG952400.4493164530494972No Hit
GAGTCCTA941440.44414582272041014No Hit
TATAGGTG936940.4420228449393069RNA PCR Primer, Index 39 (100% over 8bp)
CAAGAGAG925720.43672955367175614No Hit
GCAGTGGT914370.43137493193497345Clontech Universal Primer Mix Long (100% over 8bp)
AGAAGAGC909260.42896417272132065No Hit
AAGTGGAG909130.42890284225208886No Hit
GTGAGTCA903360.42618071296387416No Hit
TTAGGATC897780.42354822051530616No Hit
ACGGACGG893710.4216281050555083No Hit
CGATGGCA893160.42136862999337343No Hit
AGAAGTAG893140.4213591945365686No Hit
GGATATGC892410.4210148003631896No Hit
GACTAAGA874580.4126030906216183No Hit
TGTCGGCT873470.41207942276894616No Hit
CAGAGTGC867310.4091733020730359No Hit
TTGACCGA861530.40644645505641885No Hit
CACAGGCA860100.4057718198948682No Hit
GATCGGCG858730.40512549110373236No Hit
TGATTCGC856110.40388944626229006No Hit
CTAGCGGC848970.4005209881829395No Hit
GCCGGTTG848760.400421915886488No Hit
CAGCTAAC842890.39765260931424895No Hit
CGTTCATA831130.3921045607129658No Hit
TCCGCGAT829960.39155258648987895No Hit
AGGCGGTT829870.3915101269342569No Hit
CCGCTGTA829280.39123178095851224No Hit
TGAGTAAG829070.39113270866206074No Hit
AGGTAGCC820600.38713679270518414No Hit
TGCAATCG816720.3853063140850329No Hit
CGTATGAC809420.3818623723512432No Hit
GCTGTAGC808340.3813528576837784No Hit
GAACGCGC798670.3767908143186076No Hit
AGCGTGCG795770.37542267308189664No Hit
GATCTTCC793880.3745310224138333No Hit
GCCTGGTC786820.3712003061617024No Hit
GACAGTCG784960.37032280867884637No Hit
GGTCGTAT779860.36791676719359606No Hit
CGTTGTCT774450.3653644761278697No Hit
CGTGTTAG752440.3549807559140736No Hit
TCTCGAGT717240.3383743519374437No Hit
CAATACAT716520.3380346754924672No Hit
GTCGAAGC713320.3365250024036826No Hit
TCATTGCG702370.33135908980299805No Hit
CCGGCTGA702000.33118453385210733No Hit
AGCTCACG677700.31972045383414976No Hit
GTCTCCAC676650.31922509235189234No Hit
AATTATTG670840.3164840921500679No Hit
TACATGTC659990.3113653568334079No Hit
AGCCACGC641930.30284513933858015No Hit
GCGCAATC638640.3012930066941736No Hit
GCATAACG638570.30125998259535636No Hit
TGGCGCGA636590.3003258723716709No Hit
AAGGCCTG624210.2944853246094358No Hit
AGATGACT621790.2933436343360425No Hit
GCGACAAG619970.2924850077667963No Hit
TCCGGTCT602620.28429974898854266No Hit
TCGTTCTG591620.2791102477458458No Hit
TGCTCGAG582520.27481711489961475No Hit
CTTGCTTG576180.2718260750924604No Hit
CGACTCTG574690.2711231335604951No Hit
GAACAGAC558590.2635275908325479No Hit
AGGATCTT557630.26307468890591257No Hit
CGGAGCTG552530.2606686474206622No Hit
CAACAGCA539280.2544176572874137No Hit
AGACCATC539000.25428556089214505No Hit
CACATTAT538970.25427140770693774No Hit
CAATCGTA538740.25416289995368135No Hit
CAATCAGC532990.25145020612227165No Hit
GCTGCATG525890.24810061895653096No Hit
ACACTGTG525550.24794021619084758No Hit
GCGGAGTA520410.2455153037919874No Hit
GCAGATCT518130.24443966171622844No Hit
CGTACACT512050.24157128284753782No Hit
GTCCGACT510410.24079757538953578No Hit
CTCTCGAA508200.23975495741259392No Hit
GATCGATT506240.23883028264571343RNA PCR Primer, Index 42 (100% over 8bp)
GAAGGCAG500680.2362072256539503No Hit
CATACGTT496910.23442864204622602No Hit
CGCCGGTA496790.23437202930539658No Hit
CTGTAAGG482200.2274888635662196No Hit
TTGTGTAC480610.2267387447502298No Hit
AACTTGAA466040.21986501446785767No Hit
AAGCTCCA461190.2175769161926686No Hit
AATGGTCT439710.20744323558420244No Hit
GAGACTGA433140.2043436880237917TruSeq Adapter, Index 15 (100% over 8bp)
ACGCATTA433130.20433897029538922No Hit
GAGCTTAG391720.18480285698083687No Hit
CCACATAT389410.18371306171987054No Hit
GCCATCGC368840.17400869439602742No Hit
ATATTCGG356890.1683710089550977No Hit
CTCCAATG356350.16811625162136531No Hit
CACACATG355590.16775770426277897ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CAGTACCG335290.15818071560580207No Hit
CGACCTCG332180.15671350207263962No Hit
GATACGAG306320.1445134564238996No Hit
TACGTACC275500.12997341748754354No Hit
AGAACGGT249490.11770260591276674No Hit
TATACTAT234880.11081000471678484No Hit
TAGCGCTG223790.10557804391846594No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)