FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357843357
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTGTG42667011.1923376294505308No Hit
GTTACCAA42337311.1831241008618192No Hit
GTGGTCGT40636681.135599675251202No Hit
TGTTGCGT39798941.1121888731890026No Hit
GCTGCATG38206441.0676861607912984No Hit
CGCGTCGG37666771.0526049810112865No Hit
GATCTTCC37358181.043981375347985No Hit
TACATGTC36367571.0162985923474892No Hit
GTCGAAGC36119521.0093667883850084No Hit
TGTGAACA36084931.0084001643210607No Hit
TACTGCAC35648000.9961900731889233No Hit
GTCCGACT34949960.9766832139348613No Hit
GTGGACAC34790800.9722354577620397No Hit
TTCCGGTT34384020.9608679140577144No Hit
CGGAGCTG34329680.9593493725244703No Hit
CATACGTT34268070.9576276694721484No Hit
GAGACTGA33730560.9426068512989052TruSeq Adapter, Index 15 (100% over 8bp)
AGAAGAGC33206010.9279482027662735No Hit
GAGGTATA32818420.9171169272257862No Hit
CCTGGTGC32761380.9155229336840813No Hit
CCTTGGTT32676380.9131475926769824No Hit
TCGTTCTG32368290.9045379596078403No Hit
TACAGCCT32335130.9036112971631887No Hit
CGCCGGTA32059270.8959023375135619No Hit
AGGTTATC31836780.8896848125645099No Hit
CTGTGTTG31803480.8887542377934935No Hit
GTCTCCAC31803380.8887514432746617No Hit
CTCTGAGA31686870.8854955493836372No Hit
TCGGATTG31676120.88519513860921No Hit
CGTAATGT30909670.8637765490222583No Hit
GGTTCAAT30636000.8561287893350497No Hit
TGCTCGAG29319520.8193395078171033No Hit
CGCAAGTG28943950.8088441334402081No Hit
CGAGGAGT28188360.7877290285983987No Hit
TTAGGTCA27398580.7656584777679694No Hit
AATCGCGG27018760.755044336340719No Hit
GTAGTGAC26769380.7480753652777743RNA PCR Primer, Index 43 (100% over 8bp)
AGTGGATG26726940.746889371485524No Hit
TAACGGTC26463220.7395196664220876No Hit
AGCGAAGA26305280.7351060033790148No Hit
TCGCACGC25926240.7245136591986532No Hit
TGAGCGTA25816100.7214357761572195No Hit
GCACCACT25799450.7209704887717113No Hit
GTGCCACG25709140.7184467588146397No Hit
TTCGCATA25701260.7182265507306874No Hit
CTTAACAC25604990.7155362674512357No Hit
TCTATGAA25529390.7134236112143336No Hit
AATTAACA25510600.7128985211258232No Hit
GCCACTGT25023830.6992956418078763No Hit
GTCACAAT24464520.6836656185292829No Hit
TCTGATAT24441020.6830089066037909No Hit
TAAGTCCT24439970.6829795641560561No Hit
CTTCGACG24288860.6787567667492008No Hit
ACTACATA24236130.67728321696915No Hit
CCTACTCG24224610.6769612883997173No Hit
AACAGAAT24151220.6749103910289999No Hit
CGAGTTCT23999280.67066439911584No Hit
TGCGGATT23851170.666525437273941No Hit
CACACCAC23777700.6644723042881581No Hit
CACCACGG23405680.6540761353297946No Hit
TAACCTCT23141420.646691339864666No Hit
CAGATAGT23117120.6460122717885189No Hit
CTCATAGG22956800.6415320991972473No Hit
CAAGACGC22793450.6369672526853698No Hit
AGTCTACC22738380.6354283111646529No Hit
TATGAGGT22491680.6285342332064027No Hit
TCGTGAAG22460040.6276500474479956No Hit
TCCGTATG22439590.6270785683468758No Hit
TGATCCAT22208160.6206112134142538No Hit
AGGCGGTT22075340.6168995335017494No Hit
GCGCCTAA21803960.6093157682957909No Hit
GAGTATAA21597410.6035436896485409No Hit
TTGCTCTA21578560.6030169228487312No Hit
TGTAGCAC21203990.5925494936601547No Hit
CGTGCAGG21153520.5911391000057045No Hit
CCGCTGTA20786570.5808846131521173No Hit
AAGGTGTT20554410.5743968582320225No Hit
CACATTAT20421040.5706698084659428No Hit
CTAGTCCG20346420.5685845385135933No Hit
AATATTCG20245660.56576878133859No Hit
TACAAGAA20203430.5645886560358867No Hit
AGGTAGCC20187160.5641339878219396No Hit
CCGACACA20075690.561018937680042No Hit
CTTCTAGA19870560.5552865412002045No Hit
CGCACCGA19725600.5512356067015098No Hit
CACCGGAG19517850.5454299938282772No Hit
TATGCTTA19399920.5421344177698401No Hit
TCATTCAA19058530.5325942099296815No Hit
ATCTGCTG18643890.5210070170451704No Hit
ATCTAACC18381910.5136859366094088No Hit
AAGAACGT18280800.5108603986184939No Hit
GTATATCA17775910.49675115248821006No Hit
CCGCGTAC17626640.49257977422786137No Hit
GCTGACAA17570010.4909972382133672No Hit
AATTATTG17538450.4901152880700256No Hit
TACCTGCA17155920.4794254151824313No Hit
TATAGGTG16852510.47094656559462134RNA PCR Primer, Index 39 (100% over 8bp)
AACGCGCC16701750.4667335490036776No Hit
TAGCATGG16568830.4630190745723415No Hit
ATTCCACA16483700.4606401006907612No Hit
ACTTAAGG16363130.4572707493351623No Hit
CGATGGCA15837200.4425735364426508No Hit
ATAGCCTA14628010.40878249417942947No Hit
CACACATG14627930.40878025856436395ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
TGAATTGA14142320.3952097956648668No Hit
CGACCTCG9912810.27701534221857865No Hit
GTTATCCG6639000.18552810524857669No Hit
GGTCTTCC5042790.14092171620220967No Hit
TCGTGGAG4887040.1365692531215551No Hit
CGGGGCTG4386630.12258520143493959No Hit
TCGGGTTG4344200.1213994870945725No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)