FastQCFastQC Report
Fri 15 Sep 2023
EGAF00001836378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367590056
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCCGA38252821.0406380525157621No Hit
CGTTGTCT37286541.0143511607941864No Hit
TAGCGCTG36365940.9893069577486069No Hit
ATAGTTGC34790710.9464540575058429No Hit
ATTCTGGC33356360.9074336874879988No Hit
AGAGCCGC33300900.9059249415604431No Hit
GATATTGT33145000.9016838039819011No Hit
ACTCATAG32844760.8935160095843289No Hit
GTGGTACC32372920.8806799713863859No Hit
GGAATATC32194580.8758283711570262RNA PCR Primer, Index 37 (100% over 8bp)
TCATTGCG32027970.87129587640423No Hit
TTATGCTA31282550.8510173082592853No Hit
GCAGATCT31104720.8461795821810806No Hit
ACACTGTG30887340.8402659292829184No Hit
CGTGTGGT30798650.8378531871928548No Hit
GCCTGGTC30776380.8372473492590888No Hit
GGTAGCGA29927400.8141515123031511No Hit
TCTCGAGT29697340.8078929099213717No Hit
CTTGCGAT29371470.7990278714177186No Hit
CATTGTGA29366140.7988828729360405No Hit
AGGCCAGA29014060.7893048118798948No Hit
GAACGCGC28728670.7815410001188933No Hit
CCGGTAGG28554260.7767963124660804No Hit
TCCGCGAT28550380.7766907601004309No Hit
AGTTACAT28374220.7718984650662041No Hit
TCCGGCGA28352830.7713165668442348No Hit
GATGGCGT28273730.7691647132043202No Hit
ATAAGCCA27927410.7597433484435716No Hit
ATCGGCGC27916930.7594582482394464No Hit
CGATGCTC27152470.7386617117847171No Hit
AGAAGTAG27121430.7378172928595218No Hit
CTTGCTTG26248280.7140639299557113No Hit
CTGTCATT26245880.7139986398326292No Hit
TGCTCAGC26239600.7138277973438977No Hit
CAAGGTCC26068660.7091775083273744No Hit
TACGTACC25950240.7059559848376312No Hit
CAGGCTAG25831300.7027203151545536No Hit
GCGTACGA25763190.7008674358699192No Hit
AAGTGAGA25613640.6967990450753652No Hit
CCAGCGTG25605340.6965732500663729No Hit
GCGGAGTA25563080.6954235998157687No Hit
ATTCGATC25561900.6953914988385866No Hit
GCTAGTCT25275700.6876056516610449No Hit
GGTCTCGC24919940.67792747908284No Hit
TGCGCCTG24888250.6770653774159766No Hit
CCACTACA24831140.6755117445288019No Hit
GCGTCGCT24717280.6724142722729148No Hit
TCAACTTA24667210.6710521570801143No Hit
CCGGCTGA24513940.666882566594783No Hit
CACTTGCT24486650.6661401634869035No Hit
GGTCGTAT24438380.6648270158864146No Hit
TTCGACAA24325010.6617428736973233No Hit
CCACATAT24273250.6603347833761858No Hit
AGCAAGCT23789550.6471761031533453No Hit
TCTCCACG23742650.6459002253314491No Hit
ATATACAA23627040.6427551456941479No Hit
GATACACC23289330.6335680092499564No Hit
GCATGTAT23144540.6296291105328485No Hit
TCGCAACA22986270.6253234989577628No Hit
AGCTCACG22929720.6237851004326407No Hit
AAGCAGGA22878900.622402582076377No Hit
ATTAGTGC22712300.6178703593657605No Hit
GCGAGATA22538140.6131324727674353No Hit
CGGATGCG22534590.6130358977937097No Hit
ACCTCGCA22455080.6108728904244352No Hit
TATCACAT22325730.6073540248324889No Hit
TGGAAGAT22243530.6051178381169267No Hit
ACTGGTTA22155240.6027159777140435No Hit
GATTGAGG22096790.6011258911748146No Hit
GCAGTTAA21811060.5933528299797098No Hit
CTCTAGTT21805480.5932010304435439No Hit
CTTACAAG21759220.5919425633211362No Hit
CCATCTCC21729100.5911231722764557No Hit
CATCTCCG21726410.5910499929301679No Hit
TACGAGCG21689320.5900409884863697No Hit
CCATGCGG21618010.5881010557042925No Hit
GCAGTGGT21615360.5880289645267226Clontech Universal Primer Mix Long (100% over 8bp)
CTCCAATG21610220.5878891348464551No Hit
TGCTAACT21559740.5865158659242947No Hit
CTCTCGAA21369680.5813454322605506No Hit
ATCTCAGT21318450.5799517601749271No Hit
CAATCAGC21173820.5760172141326914No Hit
TCTTAGTA20594810.5602656998969526No Hit
GACATCTA20181520.5490224686600336No Hit
CAACTCGA19802220.538703908791265No Hit
AGATGACT19754170.5373967461187252No Hit
TCGGAGCT19696660.5358322315443702No Hit
CGTATGAC19362760.5267487431705715No Hit
TATTGACC19127080.5203372530839082No Hit
CGGCTTGA18812110.5117687405559197No Hit
AAGATGGC18740430.5098187422132007No Hit
ATCATCTC18453450.5020116757456573Illumina PCR Primer Index 7 (100% over 8bp)
AGTGCTCA18451550.5019599877315506No Hit
ATGGAGAG18407310.5007564731294036No Hit
ACCGACAC18304640.4979634160723869No Hit
TCGTGTCA18225830.4958194516556781No Hit
AGACTCAA18100370.49240641047156075No Hit
GGCAACAC18062230.4913688415989142No Hit
TATACGGA17855000.48573131151295346No Hit
AGCCACGC17767730.48335719941238015No Hit
CTCAATTC17722830.4821357300263857No Hit
AACCAACG17720450.48207098398766257No Hit
GTCGACCG17612630.4791378252081988No Hit
GTTCTAAT17419190.4738754412877806No Hit
GCTTATAC17367490.47246898321972014No Hit
AACGTCGA17162000.4668787884729939No Hit
CGTAGTTC16966060.4615483940076986No Hit
AACACGTA16843690.4582194138570495No Hit
GATCGGCG16606710.45177255828705004No Hit
GCTACCTC16593940.45142516042381736No Hit
ATGAGACG16589310.4512992048947047No Hit
TGGCACAG16565090.4506403187359345No Hit
GTAAGAGA16511230.44917509955710005No Hit
AGCGTTAT15786650.42946346731425183No Hit
TGTGTCGA15438370.41998878228631953No Hit
ATACTTAG15366420.4180314388047538No Hit
AACAATAG15144030.4119814927746577No Hit
CGACTTAC14078480.382994038337098No Hit
GCGGCTCC12286050.33423238195540306No Hit
CTGATTAC10374070.28221846131768047No Hit
CTTGCCGG4270730.1161818697293596No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)