FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361476673
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATCC41405231.1454468045300394No Hit
GCCTACAT37789491.0454198796944223No Hit
TCTAGTGA35442600.9804948049856596No Hit
ACAGGCTG35286050.9761639584416557No Hit
CTCGTATC34641160.958323526453393No Hit
CAATACAT34622220.9577995645655398No Hit
CACTGCTT33808350.9352844187541806Clontech Universal Primer Mix Long (100% over 8bp)
GACTCGGT33188950.9181491498346285No Hit
TTCACGGT33056580.914487226123164No Hit
TTACAATT32452580.897777987460895No Hit
TGTCTTAA32379210.8957482575922678No Hit
GCCTTAGG31667160.8760498910534124No Hit
GCCATCGC31420310.8692209580007947No Hit
TCGATTGC31101380.8603979820296731No Hit
TTAGGATC30055900.8314755071345917No Hit
TACTCAAT29605710.8190213148276929No Hit
GTCCATCC29109100.8052829456024124No Hit
GGCTCTTA29105530.8051841840427695No Hit
GTGTCGGC29070810.8042236794627132No Hit
GGACCGGC28853170.7982028206838122No Hit
GGCCGCTC28572730.7904446437128738No Hit
GGCCGTCA28536520.7894429193222103No Hit
GTATCTAG28252900.7815967698695734No Hit
ATCGCGTG28135400.7783462143351086No Hit
CGACGCCT28120630.7779376125883508No Hit
GCAGCTGG27848320.7704043463960951No Hit
CTATCCTG27787410.7687193137356335No Hit
GATACGAG27461930.7597151365836545No Hit
TGATTCGC27420180.7585601519575788No Hit
GTCCGCGG27177940.7518587513391217No Hit
GTCTTGTA27068540.748832276654267No Hit
TCGTCGAC26998640.7468985419150408No Hit
CCATTGAC26969080.7460807851354768No Hit
GCGGAAGT26961050.7458586407870363No Hit
TACACACA26910140.7444502511507846No Hit
AACGGATA26889590.7438817497360335No Hit
TAATTAGA26819500.7419427587793472No Hit
TATCAAGA26772140.7406325774166899No Hit
CACGCCGT26584150.7354319652045707No Hit
TTAGAGAT26473900.7323819758626582No Hit
TATGCCGC26205590.7249593668800863No Hit
AAGTGGAG26147120.7233418351175319No Hit
GCCGGTTG25971950.7184958792624496No Hit
CTATCGCT25874310.7157947367740656No Hit
GATTACGC25644470.7094363735056287No Hit
CTCGGCCT25241250.698281573483443No Hit
TTACAGCG24961210.690534462233473No Hit
GCACTCTC24943180.690035674860823No Hit
GAGTTCCT24677610.6826888660668845RNA PCR Primer, Index 1 (100% over 8bp)
TTATCGTC24470470.6769584824634036No Hit
TACCTCGC24067990.6658241540250095No Hit
CGACTCTG24048230.665277507409171No Hit
CAAGAGAG23552090.6515521404060284No Hit
GACCTGTG23529620.650930523530629No Hit
TTGACCGA23521740.6507125288275518No Hit
CCTTGTAG23503490.650207655308369No Hit
GACTAAGA23117040.6395167856377831No Hit
CAGTACCG22943380.6347126028793565No Hit
ACGTGGTA22819290.6312797395919376No Hit
CGTTCATA22816060.631190383895118No Hit
GGCGGAAC22733370.6289028227279275No Hit
TCTGTGTT22684130.6275406324767187No Hit
TAGATCCG22372480.6189190526272217No Hit
GGATATGC22268010.6160289629533025No Hit
CCAGGATA22265040.615946799975112No Hit
ATTACGAC22240900.6152789837146697No Hit
CCTATACC22157080.6129601618857436No Hit
CGTACACT21906110.6060172519071514No Hit
GTACTATA21853880.6045723453917039No Hit
ATACATGT21602080.5976064740421023No Hit
GAGCTTAG21482010.5942848212504158No Hit
GGCTACGG21235280.5874592079140886No Hit
TGACGGAA21136240.5847193354023151No Hit
GGCGTGTC20985330.5805445155239658No Hit
ACCGAACT20908530.5784198970980349No Hit
GGAACCTG20900540.5781988593216913No Hit
GGTTGGCC20886200.5778021532249744No Hit
ATTGACCT20568660.569017630634218No Hit
GCTGTAGC20469720.5662805245526867No Hit
AGAATGTA20468570.5662487106049026No Hit
AATCAGCT20303630.5616857605635869No Hit
CATGTATT20053950.5547785375351177No Hit
TGCCGTAG20050950.5546955446278549No Hit
TTAACCAG19720320.5455488963184079No Hit
AGGTCTTG19658370.5438350927834285No Hit
AACGGCCG19440250.5378009551393652No Hit
GACAAGGC19389050.5363845428554113No Hit
CAGCACAA19250870.5325618895468809No Hit
GCGATCCA18581980.5140575143005147No Hit
CGCGGTCG18412060.5093567960331427No Hit
CTAGGCAC18284380.5058246179000325No Hit
TTAGCAGT18115180.5011438179304035No Hit
GTGTTAAG18098320.5006773977915858No Hit
ATGCGGCA18078040.5001163657384885No Hit
ACGCATTA18064930.4997536867337495No Hit
CTCGAACA18015460.4983851336929838No Hit
TCAATCGG17935320.4961681164969669No Hit
CATGACTA17913830.49557361063794014No Hit
ATAATATG17747710.49097801671976765No Hit
CTAGCGGC17205460.47597704873199387No Hit
GCTTCTCA17149460.47442784779641917No Hit
CTACAAGC17071100.47226007305871154No Hit
GTCATACA16689630.46170697161418217No Hit
GAGAAGCT16161270.4470902607870356No Hit
ATACGTTA16035960.44362364705066326No Hit
ACCAGGCC15885470.43946044617933067No Hit
CCTCCATC15726730.435069014813025No Hit
CCATAAGA15703300.4344208402073016No Hit
TTGCAGAA15675660.43365619888838575No Hit
CATCAGTG15674080.4336124892905606No Hit
AAGCCTGT15594410.43140847431668156No Hit
CCTGTGCG14919480.4127370066836927No Hit
AGATGGAC14634850.4048629162856105RNA PCR Primer, Index 16 (100% over 8bp)
ATGTCAAC14197900.3927749993427653No Hit
AATTCATT13104880.3625373635105909No Hit
ATTAGGCT12373240.34229705328730853No Hit
TTCAGTAA11669520.3228291303876198No Hit
AATCCAAG8464560.23416614770049074No Hit
CGATCTAA7662900.2119887830216917No Hit
CGTGGTCC4207110.11638676335830943No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)