FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22658795
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAA3978861.7559892306717988No Hit
GTTACA3560711.5714472018481123No Hit
GACCAT3292481.4530693269434671No Hit
AGCCTA3045881.3442374142137745No Hit
AGACGG2957591.3052724118824501No Hit
TATAAG2839531.2531690233306758No Hit
GGATTC2772721.223683783714006No Hit
GAGTGA2687571.1861045567515838No Hit
TATGGT2566941.1328669507800393No Hit
AGGTGT2535501.1189915439016065No Hit
ACTAAT2456661.0841971075690477No Hit
AGTCAA2384871.0525140458704887No Hit
TATTCT2291721.0114041810255134No Hit
ACGTAA2255050.9952206196313617No Hit
CTAGTT2212470.9764287995014739No Hit
CCTTAT2136570.9429318725907534No Hit
ACAGCC2074130.9153752439174281No Hit
AGGCTT2069670.9134069132979048No Hit
TTCTGT2067420.9124139213934368No Hit
GGTGGT1976020.8720763835852701No Hit
AAGCAT1974330.8713305363325808No Hit
AACAAT1969590.8692386333871682No Hit
CTGTAT1915030.8451596830281575No Hit
GCACAT1913740.8445903676695959No Hit
GCTGTC1905330.8408787845955621No Hit
GGCGTT1891850.8349296597634606No Hit
AATCGG1885460.8321095627547714No Hit
AACTAG1865710.823393300482219No Hit
AGATGC1780850.7859420591430392No Hit
ATCAGC1768070.7803018651256609No Hit
TACGGC1766730.7797104832803333No Hit
CTTAGC1751200.7728566324908275No Hit
CCTGCT1690740.7461738366934341No Hit
CCGACT1678270.7406704548940047No Hit
CACCTA1665900.7352112060681073No Hit
TCTTCA1639090.723379155864202No Hit
GTCTCA1615910.713149132599505No Hit
ATTGAA1610850.7109160041387902No Hit
ATACCT1608790.7100068648840329No Hit
TACTCG1582170.6982586673298381No Hit
CCTGAC1565600.6909458336156005No Hit
TGACAC1549970.6840478498525627No Hit
GTCTGC1547980.6831696036792777No Hit
TTAGCG1545550.6820971724224523No Hit
AGTAGG1484270.6550524862420972No Hit
CCTCGG1467240.6475366408496127No Hit
CCATGA1466940.6474042419290169No Hit
TCCAGA1462420.6454094315253746No Hit
AGAACG1445670.6380171584587795No Hit
CAAGCT1426240.6294421217015291No Hit
ATCCAC1410450.6224735251808403No Hit
ACGCTA1405580.6203242493698363No Hit
TGCGAC1387510.61234942105262No Hit
AAGTTG1376740.6075962998032332No Hit
GGCCAG1367440.6034919332647654No Hit
TCAAGC1364660.6022650366005783No Hit
TTCACA1364580.6022297302217527No Hit
TAGAGC1351260.5963512181473022No Hit
ACCTGC1349760.5956892235443235No Hit
TGGCAT1313740.5797925264781292No Hit
CATGAG1310420.5783273117568697No Hit
ACCGCT1284690.566971897667109No Hit
CTATTA1284110.566715926420624No Hit
TTCTAC1283520.5664555418767856No Hit
GTACGC1277560.5638252166542836No Hit
TGTGTG1266150.5587896443742926No Hit
TTGAAT1237690.5462294001071107No Hit
GAACCG1233100.544203696621996No Hit
CTATGC1218270.5376587766472136No Hit
CACATT1209540.5338059680578777No Hit
CAGCCT1196540.5280686814987292No Hit
AGTTCC1191350.5257781801724231No Hit
CAGTAG1190800.5255354488179976No Hit
GGTACC1189340.5248911074044317No Hit
GGCCGT1187660.5241496734490956No Hit
CTCCGC1166790.5149391218729857No Hit
ATGGCC1163100.5133106151496581No Hit
AATGTA1162950.5132444156893604No Hit
ACTAGC1156420.5103625325177266No Hit
GTACTT1148100.5066906691198716No Hit
CATTAA1140450.5033144966446803No Hit
CCGTCG1138610.5025024499316932No Hit
GCGCAG1137820.5021537994407911No Hit
GAATAC1131330.49928956945857006No Hit
TCCTCC1124050.4960766889854469No Hit
GACTAA1115780.49242689207435786No Hit
CATAAC1114100.49168545811902176No Hit
ACCAGG1091760.48182615183199284No Hit
GAGATA1085250.4789530952550654No Hit
ATGCCG1082400.4776953055094059No Hit
ATTGCT1079800.47654784819757623No Hit
GCGTAT1075320.4745706909833466No Hit
CGTCAG1054740.4654881250304793No Hit
GATTCA1054580.46541751227282824No Hit
GATCCT1045270.4613087324370073No Hit
CTTCAC1028340.4538370200180548No Hit
GGACTA1025460.45256599038033574No Hit
CAGTCC1016400.44856754297834467No Hit
TCGTGG993300.43837282609247313No Hit
AACATA991130.4374151405668307No Hit
AGAGGT990190.43700029061563067No Hit
AACCTT982900.4337829968451544No Hit
CGTGAA979140.4321235970403545No Hit
AGGTCA978060.4316469609262099No Hit
TAATGA951390.4198766968852492No Hit
TCATGT944540.41685358819831325No Hit
TGATAA943030.4161871802979814No Hit
GAGCCA933200.41184890899979454No Hit
CGCTTG929440.4101895091949947No Hit
CGACAA919590.4058424113021015No Hit
CGTCTC919590.4058424113021015No Hit
AAGGCG916730.40458020825908886No Hit
ATTCGA915520.40404619927935265No Hit
TCGGAC912610.40276192974957403No Hit
GCTCAA912240.4025986377475059No Hit
TGCATA900660.39748803941251065No Hit
TACGAA900370.39736005378926814No Hit
TATTGG896610.3957006539844683No Hit
CCGCTC893800.3944605174282216No Hit
TGTGAT888760.3922362155622133No Hit
CTGTTC881710.38912484092821353No Hit
CAGGAC876190.38668870078925205No Hit
AAGCGC873360.3854397376382989No Hit
ACACGA861810.38034237919536323No Hit
GCGGCT852130.3760703073574742No Hit
GAGTCG851590.3758319893004019No Hit
ATGTGA843250.3721512993078405No Hit
CGAATT843120.37209392644224903No Hit
CTACCG838780.37017855539096406No Hit
GGTAAT834110.3681175455270238No Hit
TTCGTT830040.3663213335042751No Hit
TCTCGC829090.36590207025572186No Hit
GACACG818110.36105626976191807No Hit
TAAGTC810530.3577109903681992No Hit
CGAGCC809810.35739323295876946No Hit
GTAAGA809160.35710636863081197No Hit
AGTTAG800150.35312998771558685No Hit
CGAGAG788790.3481164819223617No Hit
AGTGCA757940.3345014595877671No Hit
CATACG755390.3333760687627034No Hit
GAAGAT750250.33110763392316317No Hit
GGCAGG742100.32751079658031246No Hit
TTCCAT733100.3235388289624404No Hit
TGCGGA717450.3166320186046963No Hit
ATCCGG701770.3097119683548926No Hit
GCTTCT701720.3096899018681267No Hit
GGATCG699860.3088690285604332No Hit
CAAGGA695080.3067594724256078No Hit
TCCACG694850.3066579665864844No Hit
GACCGA668590.29506864773700453No Hit
CGTACT659950.2912555588238474No Hit
GCCATA654560.28887679155047746No Hit
CCAGCG646500.28531967388380536No Hit
TCTCTG643090.2838147394863672No Hit
GACTTG639090.2820494205450908No Hit
GAGCAC635890.2806371653920696No Hit
TTACGT635760.28057979252647813No Hit
ATTCAT628180.27723451313275926No Hit
GTGGTG620000.27362443589784896No Hit
CTCGTC610340.2693611906546663No Hit
TTAGTA602660.26597177828741553No Hit
GTTGGC602440.2658746857456454No Hit
CTGGAG582110.2569024522266078No Hit
ACGAAC568670.250970980583919No Hit
CCGGAA563210.2485613202290766No Hit
GAGAAG545960.24094838229482196No Hit
GTCGAG542410.2393816617344391No Hit
CCACTT538220.237532490143452No Hit
CGAAGC524200.23134504725427807No Hit
TCAATG517940.22858232311118043No Hit
GGAGGC512440.22615500956692536No Hit
CAACGG511550.22576222610249133No Hit
AATTGC482160.21279154518146268No Hit
TACAGT462890.20428712118186337No Hit
AGGACT457220.20178478158260402No Hit
CTGAGG449140.1982188373212256No Hit
AAGAGG420930.18576892548787347No Hit
CGTTGC405880.17912691297132086No Hit
AACTCT331290.14620812801386834No Hit
GCCTGG266760.1177291201937261No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)