FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22303383
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGT4848552.1739078775627894No Hit
ACCAGG4623672.0730801242125465No Hit
AACTCT3752381.6824263834773405No Hit
ATTCAT3383371.516976146623138No Hit
GGTAAT2892201.2967539498380134No Hit
TTCTAC2876981.289929872970392No Hit
ACTAGC2841831.2741699319784805No Hit
ATCCGG2824071.2662070144246726No Hit
CCGTCG2615641.1727548237861494No Hit
CGTTGC2472691.1086614080025436No Hit
CCATGA2335341.047078822078247No Hit
TAGAGC2218760.9948087247571366No Hit
AGTGCA2149910.9639389683618849No Hit
AATCGG2131960.9558908619378504No Hit
CGAATT2109180.9456771647601621No Hit
CGTACT1955360.8767100488746482No Hit
CAAGGA1916310.8592014942307182No Hit
TCTTCA1882060.8438450794661958No Hit
GCATAA1873990.8402267942939419No Hit
CCACTT1855620.8319903756304594No Hit
AACATA1849170.8290984376675054No Hit
AACTAG1845760.8275695216281763No Hit
GGTACC1835280.8228706828914698No Hit
CTATTA1809440.8112849965406593No Hit
CCTTAT1750380.7848047087744491No Hit
TACGAA1678930.7527692099445182No Hit
AGACGG1629560.7306335545598621No Hit
TGTGAT1595810.7155013210327779No Hit
CCTGCT1594180.7147704901987291No Hit
GCACAT1563400.7009698932220283No Hit
GATCCT1560620.6997234455418714No Hit
ACCTGC1548060.6940920128574217No Hit
GTCTCA1520620.6817889465468087No Hit
TTAGTA1490600.6683291050510141No Hit
CCTCGG1480550.6638230621785045No Hit
CAGGAC1466860.6576849798974442No Hit
GCTGTC1442430.6467314846362097No Hit
TTCTGT1433240.642611033492094No Hit
TACGGC1431600.6418757190332964No Hit
ACAGCC1411850.6330205601544842No Hit
GTACTT1400130.6277657519489308No Hit
AGGCTT1399030.6272725532265665No Hit
TGGCAT1396500.6261381961651289No Hit
GAGTCG1390210.6233179961981553No Hit
GCTCAA1388400.6225064601186286No Hit
TCGTGG1383680.6203901892372112No Hit
GTGGTG1373570.615857244616209No Hit
ATGGCC1369990.614252106956151No Hit
ATCCAC1358510.609104905744568No Hit
CTGGAG1355120.6075849569547365No Hit
GTTGGC1341650.601545514418149No Hit
GTTACA1334330.5982635011020526No Hit
AGTCAA1326780.5948783644167345No Hit
TATTCT1324100.5936767529840652No Hit
CATGAG1314350.5893052188540188No Hit
CACCTA1312210.5883457231577828No Hit
TAAGTC1287520.5772756536530804No Hit
GGACTA1285790.5764999865715439No Hit
CACATT1280580.574164018077437No Hit
AGGACT1276070.5721419033157437No Hit
TATTGG1260380.5651070960849303No Hit
TTCCAT1257980.5640310261452265No Hit
CATTAA1254520.5624796919821535No Hit
AAGCAT1251650.5611928916792578No Hit
ACTAAT1251200.5609911285655633No Hit
TCCTCC1237610.5548978825319908No Hit
AGCCTA1234860.5536648857260802No Hit
TCCAGA1234500.5535034752351247No Hit
GAAGAT1219420.546742169113986No Hit
GAGTGA1214770.54465728360581No Hit
CGTCAG1214700.5446258982325686No Hit
TACAGT1205870.5406668575794085No Hit
GACCAT1204840.5402050442302856No Hit
GAGCAC1197380.5368602601677064No Hit
GTAAGA1195440.5359904369664459No Hit
AACCTT1190150.5336185994743489No Hit
CAGTAG1178370.5283368895203028No Hit
CTGAGG1168500.5239115518932711No Hit
CGAGAG1166290.5229206708237939No Hit
GAGATA1165240.5224498902251734No Hit
GCTTCT1165080.5223781522291933No Hit
AGATGC1153290.5170919586503985No Hit
TATAAG1152860.5168991627862015No Hit
TGCATA1152460.5167198177962509No Hit
TATGGT1132660.5078422407936948No Hit
GACCGA1128140.5058156424072527No Hit
TCGGAC1110610.49795584822266653No Hit
ATTGCT1104420.49518048450318053No Hit
TGTGTG1102730.4944227519206391No Hit
AGTAGG1102590.49435998117415636No Hit
CATAAC1088320.4879618486576678No Hit
GGTGGT1085500.4866974664785158No Hit
ACCGCT1084150.48609217713743247No Hit
CTGTAT1075320.48213313648427236No Hit
AATTGC1074600.48181031550236125No Hit
AATGTA1067460.4786090074317425No Hit
GGAGGC1065690.47781540585121096No Hit
GACACG1065010.4775105193682949No Hit
CGACAA1045530.46877641835769934No Hit
CAAGCT1043990.46808594014638943No Hit
TTCACA1035670.46435556435541636No Hit
GGCGTT1027310.46060725406544833No Hit
ATCAGC1022570.45848201593453336No Hit
CGAAGC1016880.45593083345248564No Hit
TTCGTT996440.44676630446600857No Hit
ACACGA995150.4461879168734178No Hit
CCGACT987200.4426234351981491No Hit
GGCCGT973600.4365257055398277No Hit
CTATGC968750.43435114753667636No Hit
CTTAGC966180.43319885597624364No Hit
CTCCGC965910.43307779810802693No Hit
CGAGCC954460.42794404777069023No Hit
CAGTCC948610.4253211272926623No Hit
CGTGAA943210.4228999699283288No Hit
TCTCTG942140.4224202220802109No Hit
AACAAT940760.4218014818648812No Hit
CTAGTT940360.4216221368749306No Hit
GACTAA939540.4212544796455318No Hit
GGATTC939230.42111548727832004No Hit
ACGTAA926380.41535402947615613No Hit
ATTCGA925370.4149011833765308No Hit
ATGCCG925320.41487876525278694No Hit
GGATCG894230.4009391759088744No Hit
AGGTGT890700.3993564563725602No Hit
TGATAA888460.39835212442883666No Hit
GTACGC886810.3976123263452903No Hit
GGCCAG881900.3954108665936463No Hit
ACGAAC872940.39139353881875233No Hit
GTCGAG869520.38986013915467443No Hit
TAATGA864960.3878156062692373No Hit
AGGTCA864530.3876228104050404No Hit
TTGAAT863570.3871923824291588No Hit
ATTGAA861920.38645258434561247No Hit
TCATGT808180.36235758494574566No Hit
CGTCTC796190.3569817188719756No Hit
CTTCAC787740.3531930559592686No Hit
AGAGGT786980.3528523004783624No Hit
TCAAGC786800.35277159523288465No Hit
GAGCCA785500.3521887240155451No Hit
TACTCG782720.3509422763353882No Hit
GCCATA754340.3382177492983912No Hit
GATTCA749470.3360342240457423No Hit
TCCACG745160.3341017817790243No Hit
GCGGCT740600.3320572488935871No Hit
ATGTGA735630.32982888739345056No Hit
CTGTTC733210.3287438502042493No Hit
TGCGGA730320.327448082651856No Hit
GCCTGG721320.32341282037796687No Hit
GAACCG717960.3219063224623816No Hit
TCTCGC711650.31907715524591046No Hit
CAGCCT705640.3163824967719023No Hit
GCGTAT699560.3136564529246527No Hit
GACTTG693400.3108945400794131No Hit
ATACCT689630.3092042135491284No Hit
GTCTGC677950.3039673398425701No Hit
GAGAAG676980.3035324282419398No Hit
AAGCGC644030.28875888469475686No Hit
CTACCG636420.28534684626094614No Hit
CCAGCG635200.2847998440415967No Hit
TTAGCG628990.28201551307261324No Hit
ACGCTA623950.2797557661992353No Hit
GCGCAG611770.2742947112552387No Hit
CATACG609380.2732231249402837No Hit
AAGGCG590430.26472665604137274No Hit
CGCTTG583070.2614267082262812No Hit
AGAACG552740.24782787436327483No Hit
TGCGAC540200.24220540892832265No Hit
CCGGAA511110.22916254453416326No Hit
AGTTCC502460.22528420912648095No Hit
CTCGTC490110.21974693256175534No Hit
GGCAGG430400.19297520918687538No Hit
GAATAC411480.1844921911622107No Hit
CCTGAC390650.17515280081053175No Hit
TGACAC363720.16307839936210575No Hit
AGTTAG333490.14952440174658704No Hit
AAGAGG312790.14024329851664208No Hit
CAACGG276010.12375252669068186No Hit
TCAATG238220.1068089087650963No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)